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All Journal HAYATI Journal of Biosciences Jurnal Presipitasi : Media Komunikasi dan Pengembangan Teknik Lingkungan BIOTROPIA - The Southeast Asian Journal of Tropical Biology AGRIVITA, Journal of Agricultural Science Jurnal Biodjati BIOEDUSCIENCE Marine Research in Indonesia Jurnal Kelautan Nasional Makara Journal of Science
Dian Hendrayanti
Departemen Biologi, Gedung E, FMIPA Universitas Indonesia, Kampus UI Depok, Depok 16424
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Veterinary

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Journal : Makara Journal of Science

 1 
Phylogeny of Indonesian Nostoc (Cyanobacteria) Isolated from Paddy Fields as Inferred from Partial Sequence of 16S rRNA Gene Hendrayanti, Dian; Kusmadji, Lestari Rahayu; Yuliana, Pratiwi; Amanina, Mardhotillah Asma
Makara Journal of Science Vol. 16, No. 3
Publisher : UI Scholars Hub

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In order to collect Indonesian Nostoc, isolation of soil microflora from several paddy fields in West Java, Bali, and South Celebes was carried out. Fast-growing isolates of Nostoc were selected to describe and perform molecular identification using partial sequences of 16S rRNA. The results showed that partial sequences of 16S rRNA could not resolve the phylogeny of the isolates. However, it supported the morphological studies that recognize isolates as different species of Nostoc. Potential use of Nostoc as a nitrogen source for paddy growth was carried out using six strains as single inoculums. A total biomass of 2 g (fresh weight) for each strain was inoculated, respectively, into the pot planted with three paddy plants. This experiment was conducted in the green house for 115 days. Statistical analyses (ANOVA; α = 0.05) showed that of six strains tested in this study, only strain GIA13a had influence on the augmentation of root length and the total number of filled grains.
BIODIVERSITAS CYANOBACTERIA DARI BEBERAPA SITU/DANAU DI KAWASAN JAKARTA-DEPOK-BOGOR, INDONESIA Prihantini, Nining Betawati; Wardhana, Wardhana; Hendrayanti, Dian; Widyawan, Arya; Ariyani, Yuni; Rianto, Ronny
Makara Journal of Science Vol. 12, No. 1
Publisher : UI Scholars Hub

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Biodiversity of Cyanobacteria from several ponds/lakes of Jakarta-Depok-Bogor, Indonesia. Research about the exploration on Cyanobacteria diversity from several ponds/lakes of Jakarta-Depok-Bogor area was done. Five research locations were Sunter 2 Lake of North Jakarta, Babakan Pond of South Jakarta, Ulin-Salam dan Agathis Pond at UI Campus of Depok, dan Lido Lake of Bogor. Samples were taken by purposive sampling, and samples analysis using subsample methode. The sampling times were on August to September 2006. Three types of samples which were taken were planktonic samples, epifitic samples, and waters bank soil samples. The results showed that several toxic potential Cyanobacteria were found in several waters, even they were dominant at Sunter 2 Lake dan Agathis Pond (UI Campuss). Based on similarity levels of Cyanobacteria, the five of waters could be classied into 3 group, i.e. Sunter 2 Lake and Agathis Pond, Babakan dan Ulin-Salam Pond, dan Lido Lake. Lido Lake waters was very unique on distribution and diversity of Cyanobacteria. Based on diversity Index (H’) of Cyanobacteria, several waters condition could be catagories as moderate waters, i.e. Babakan, Situ Ulin-Salam, and Situ Agathis Pond. The epifitic and waters bank soil Cyanobacteria which were found were also found as planktonik species except Oscillatoria sp. 3. At least 26 isolate of Cyanobacteria were acquired such as Miicrocystis, Oscillatoria, Gloeocapsa, Merismopedia, Planktothrix, dan Arthrospira.
Characteristics and Potential Indigenous Bacteria Isolated from Shallot Farming in Brebes Regency as Diazinon Pesticide Remediation Agents Purbalisa, Wahyu; Hendrayanti, Dian; Yusuf, Wahida Annisa
Makara Journal of Science Vol. 29, No. 3
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Diazinon is an organophosphate pesticide that is widely used by shallot farmers in Brebes Regency. Due to its toxicity, diazinon residue in agricultural lands as well as plants’ cultivation is harmful to humans and the environment. Using indigenous soil bacteria as a remediation agent is an alternative solution to overcome diazinon contamination. Firstly, this study aimed to characterize isolates from diazinon-contaminated shallot farming. The second objective was to test the diazinon tolerance of the isolates in various diazinon concentrations. Isolation was carried out using nutrient agar (NA) media containing diazinon concentrations of 5, 25, 50, and 100 ppm. The characterization of isolates was based on morphology and physical chemistry, including gram staining, catalase-oxidase, and carbohydrate fermentation tests. A hypersensitive test was conducted to ensure the non-pathogenic properties of the isolates. Diazinon degradation was tested using gas chromatography. This study successfully obtained 10 isolates, six of which were Gram-positive bacteria. The colonial shapes were various, including circular, filamentous, and irregular. All isolates were neutrophilic bacteria, positively reacted to catalase tests, and could use glucose in carbohydrate fermentation tests. The hypersensitive test demonstrated that all isolates were non-pathogenic. All isolates adapted to various concentrations of diazinon up to 50 ppm. The highest diazinon degradation (82.2%) was performed by Gram positive-filamentous strain KD1-D100.1. The application of indigenous isolates in this research will be studied further by testing the single and consortium inoculation for soil remediation.
Co-Authors Agustin, Zenitha Lintang Amanina, Mardhotillah Asma Andi Salamah Anggi Septiani Ariyani, Yuni Ariyani, Yuni Arya Widyawan Arya Widyawan Arya Widyawan, Arya Atria, Mega DWI ANDREAS SANTOSA Fadilah, Nurrahmi Feni Andriani Hamim Hamim Hanupurti, Dhiti Aliya Humaida, Milka Iman Rusmana Khoiriyah, Istatik KRISTIAWAN, ONIE Kusmadji, Lestari Rahayu Lestari Rahayu Kusmadji Mardhotillah Asma Amanina Nining Betawati Prihantini Nirwawan, Rino NUNIK SRI ARIYANTI Pratiwi Yuliana Prihantini, Nining Betawati Prihantini, Nining Betawati Purba, Eliza Pricilia Purbalisa, Wahyu Putrika, Afiatry Riani Widiarti Rianto, Ronny Rianto, Ronny Ronny Rianto Sani, Lalu M. Iqbal Tsabitudinillah, Sarah Wahida Annisa Yusuf Wahyu Purbalisa Wardhana wardhana Wardhana, Wishnu Winarni, Nurul Laksmi Wisnu Wardhana Yasman Yasman Yuliana, Pratiwi Yuni Ariyani Yusuf, Wahida Annisa