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All Journal Jurnal Ilmu dan Teknologi Kelautan Tropis JOURNAL OF COASTAL DEVELOPMENT ILMU KELAUTAN: Indonesian Journal of Marine Sciences Management of Aquatic Resources Journal (Maquares) Journal of Marine Research Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Jurnal Kelautan Tropis Aquacultura Indonesiana

Ali Djunaedi

Department Of Marine Sciences, Faculty Of Fisheries And Marine Sciences, Diponegoro University, Semarang

Author-ID : 255223

Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Library & Information Science

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Pemberian Pelet dengan Ukuran Berbeda terhadap Pertumbuhan Kepiting Bakau (Scylla serrata ForsskÃ¥l, 1775) Aditya, Bagus Pitra; Sunaryo, Sunaryo; Djunaedi, Ali
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Mud crab (S. serrata ForsskÃ¥l, 1775) is one of the fisheries resources that has high economic value and potential to be cultivated. This study examines provision pellets of different sizes, adapted to the mangrove crab eating patterns. The purpose of this study is investigated the effect of different sizes pellets for the growth of mud crab (S. serrata ForsskÃ¥l, 1775). This research was conducted at the Laboratory of Coastal Area Development (LPWP), UNDIP, Faculty of Fisheries and Marine Science, University of Diponegoro, Jepara, for 35 days, in October-November 2011. The research method in this study was used experimental laboratory with completely randomized design (CRD) with 3 treatments and 10 replications in each treatment, ie: treatment A (Ã˜ pellets + 10 mm), treatment B (Ã˜ pellets + 5 mm) and treatment C (Ã˜ pellets + 1 mm). Initial weight of mud crab test is 50-55 g and have male gender. Growth of mud crab can be seen from the observation from SGR and supported by some other data, ie: the amount of feed intake, FCR, PER, the amount of energy consumption in feed and water quality parameters. These results indicate that administration of treatment different size pellets effect is very significant (p<0,01) on SGR, FCR, PER. SGR data suggest that on average in treatment A (5,42+0,64) reached the highest value, but not significantly different (p>0,05) with treatment B (5,17+0,52), whereâ€™s treatment C (4,02+0,51) reached the lowest value. FCR data show that, in treatment A (5,21+1,12) reached the lowest value, but not significantly different (p>0,05) with treatment B (5,42+1,15), while treatment C (7,16+1,55) reached the lowest value. PER data showed that, in treatment A (0,62+0,13) reached the highest value, but not significantly different (p>0,05) with treatment B (0,60+0,13), while treatment C (0,46+0,11) reached the lowest value.

Pemberian Enzim Papain pada Pakan Komersil Terhadap Pertumbuhan dan Efisiensi Pakan Ikan Kerapu Macan (Epinephelus fuscoguttatus) Fadli, Jauharul; Sunaryo, Sunaryo; Djunaedi, Ali
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Tiger grouper (E.fuscoguttatus) is one of the coral reef fishes, who has significant economical value. Relatively expensive value and highly demand of this species on the market prospective make a lot of fish has been developed by fishermen through aquaculture. This study aimed to determine the effect of the enzyme papain in commercial feed on the growth and feed efficiency of Tiger Grouper (E.fuscoguttatus). This study was conducted at the Center for Development of Brackish Water Aquaculture (BBPBAP) Jepara in June-August 2012. The method, that was used in this study was experimental with completely randomized design (CRD). This study used 4 treatments, each was replicated 3 times. Treatment was the use of enzyme papain in the commercial feed with a protein content of 46-48% as much as 3%in treatment A, 4% in treatment B, 5% in treatment C, and 0% as control in treatment D. The respon of treatment showed significant by different on growth. The and in grafik was reached by treatment C ( the used of enzyme papain 5% in the artificial feed), i.e : 3,24% day -1, and than followed by treatment B of 3,15% day -1, treatment A of 3,03 day -1, and treatment D as control of 2,92% day -1. The results were obtained in this study showed that the use of the enzyme papain in artificial diets with specific concentration had a positive by effect on growth and feed efficiency. Papain enzyme capable of hydrolyzing the protein in the feed is higher amino acid to be absorbed and utilized for growth.

Pengaruh Perbedaan Lama Perendaman dalam Larutan Pupuk Fosfat Terhadap Pertumbuhan Rumput Laut Gracilaria verrucosa (Hudson) Papenfuss Di Pertambakan Desa Wonorejo, Kaliwungu-Kendal Sari, Ayu Permana; Sunaryo, Sunaryo; Djunaedi, Ali
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Species G. verrucosa is naturally harvested, through harvesting and retrieval in nature. These activities led to excessive exploitation and dangerous continueties of seaweed population. There for it would require an effort to increase the production of G. verrucosa, including through aquaculture cultivation in area with good land management and proper use of fertilizer. One of the problems in the evironmental factors that affect production of G. verrucosa is limited nutrients. This problem can be solved by ensuring availability of nutrients. Added of inorganic elements for alternative to achieve an optimal level of production. The research design was Completely Randomized Design (CRD) with immersion time difference in G. verrucosa using SP 36 fertilizer. The immersion treatments were: A. control, B. 10 minutes, C. 20 minutes, D. 30 minutes, E. 40 minutes, F. 50 minutes, G. 60 minutes. This study used P (phospat) obtained from SP 36 fertilizer with concentration 0,2 g/L. Plantation was done by using a longline method of 72 ties and mantained for 35 days. The result show that the immersion period of G. verrucosa significantly effect (p<0,01), on the growth of G. verrucosa. This can be seen from the highest average specific growth rate of G. verrucosa in the treatment G (2,36 % per day) and the lowest average specific growth rate G. verrucosa in treatment A (1,14 % per day).

Uji Penggunaan Bacillus sp. sebagai Kandidat Probiotik Untuk Pemeliharaan Rajungan (Portunus sp.) Linggarjati, Kharisma Firdaus; Djunaedi, Ali; Subagiyo, Subagiyo
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Blue crab is one of the economically marine commodities and have high potential export. During this blue crab requirement derived from nature catches because in blue crab aquaculture not yet good branch out. To low survival rate of blue crab caused by bacterium vibrio disease, in addition to the content of nitrogen and phosphate can also affect the blue crab medium. This research as a purpose to test the effect of probiotic bacillus aplication to water quality and the total number of bacteria in the blue crab medium. the research was conducted with 2 stages, that is isolation and selection bacillus bacteria stages as probiotic and testing phase the effect of probiotic bacillus on water quality and the total number of bacteria. The result showed that probiotic bacillus with density 106 cfu/ml to reduce the content of ammonia (NH3), nitrite (NO2) and the total number of bacteria. While probiotics with density 105 cfu/ml to reduce the total number of bacteria in the blue crab medium, but have not been able to reduce the content of ammonia (NH3) and nitrite (NO2).

Metode Lepas Dasar dengan Net Bag pada Pertumbuhan Kappaphycus alvarezii (Doty) Wibowo, Ichsan Suryo; Santosa, Gunawan Widi; Djunaedi, Ali
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

ABSTRAK: Parameter keberhasilan dari kegiatan budidaya rumput laut diukur berdasarkan dari hasil produksi thallus yang dibudidayakan. Metode budidaya yang digunakan dalam kegiatan budidaya rumput laut merupakan salah satu faktor penting yang dijadikan sebagai acuan keberhasilan. Penelitian ini bertujuan untuk mengkaji pertumbuhan dari rumput laut K. alvarezii yang dibudidayakan dengan metode lepas dasar menggunakan net bag yang dimodifikasi. Materi yang digunakan dalam penelitian ini adalah bibit rumput laut K. alvarezii (F2) yang diperoleh dari pembudidaya di Perairan Pulau Kemujan, Kepulauan Karimunjawa. Metode penelitian yang digunakan adalah eksperimen dengan rancangan percobaan berpola Rancangan Acak Lengkap. Perlakuan yang diujikan adalah rumput laut K. alvarezii yang dibudidayakan dengan perlakuan A (satu lapis jaring), perlakuan B (dua lapis jaring), dan perlakuan C (tiga lapis jaring). Penanaman bibit dilakukan selama 42 hari sejak penanaman. Parameter pengamatan meliputi laju pertumbuhan spesifik dan parameter kualitas perairan. Hasil penelitian menunjukkan terdapat pengaruh sistem budidaya lepas dasar menggunakan net bag (P < 0,05) terhadap laju pertumbuhan spesifik K. alvarezii. Metode net bag dengan satu lapis jaring memiliki pertumbuhan terbaik pada 4,95 Â± 0,70 %g/hari.Â ABSTRACT: The success parameters of seaweed farming activities are measured based on the results of cultivated thallus. The cultivation method used in seaweed farming activities is one of the important factors used as a reference for success. This study aimed to assess the growth of K. alvarezii that was cultivated by employing off bottom method in a modified net bag system. The material used in this study was K. alvarezii (F2) seaweed seeds obtained from cultivators in Kemujan Island, Karimunjawa Islands. The research method used was an experiment with a completely randomized design patterned. The treatments tested were K. alvarezii which was cultivated by treatment A (single net), treatment B (double net), and treatment C (triple net). Planting of seeds was carried out for 42 days after planting. Observation parameters include specific growth rate and water quality parameters. ANOVA test results showed that there was a significant influence (P <0.05) on the absolute weight and specific growth rate of K. alvarezii. Results showed that single net bag method given the best growth rate at 4.95 Â± 0.70 %g/day.

Ekologi Fitoplankton : Ditinjau dari Aspek Komposisi, Kelimpahan, Distribusi, Struktur Komunitas dan Indeks Saprobitas Di Perairan Morosari, Demak Putri, Cinthya Ruhanto; Djunaedi, Ali; Subagyo, Subagyo
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Perairan Morosari yang terletak di Kecamatan Sayung, Kabupaten Demak merupakan tempat terjadinya percampuran antara air darat dan air laut. Disekitar perairan Morosari terdapat pemukiman penduduk, industri wisata dan aktifitas nelayan. Aktivitas masyarakat dan wisatawan tersebut akan menimbulkan perubahan yang mengarah pada peningkatan pencemaran yang berimbas kepada keberadaan fitoplankton. Tujuan dari penelitian ini adalah untuk mengetahui komposisi, kelimpahan, distribusi, struktur komunitas dan indeks saprobitas fitoplankton di perairan Morosari. Metode yang digunakan dalam penelitian ini adalah deskriptif eksploratif dan penentuan lokasi menggunakan metode purposive sampling. Penelitian ini terbagi atas 3 sub-area di 3 area yang berbeda. Pengambilan sampel zooplankton dengan cara aktif dengan menarik planktonet secara horizontal. Hasil penelitian diperoleh 24 genera fitoplankton dari 3 kelas di perairan Morosari. Kelimpahan fitoplankton bervariasi antara 12.556 â€“ 19.108 individu/m3. Distribusi fitoplankton tidak merata dan cenderung mengikuti arah arus. Indeks keanekaragaman menunjukkan nilai 1,70â€“2,25 yang termasuk dalam kategori sedang. Indeks keseragaman fitoplankton menunjukkan nilai 0,71â€“0,81 yang termasuk dalam kategori Tinggi. Indeks dominansi di perairan Morosari berkisar antara 0,18â€“0,29 dan termasuk ke dalam kriteria tidak ada jenis yang mendominasi. Indeks Saprobik menunjukkan nilai 0,6â€“1,3 yang termasuk dalam kategori Î²â€“Mesosaprobik.Â Morosari waters located in District Sayung, Demak Regency is waters area there is a mixture of land and sea water. Surrounding the Morosari waters there are residential, tourist industry and fishing activities. These activities will be affected changes that lead to increased pollution that affects the presence of phytoplankton. The purpose of this research was to know the composition, abundance, distribution, community structure and saprobity index of phytoplankton in Morosari waters, Sayung Subdistrict, Demak District. This research used deskriptive eksplorative method and determination location used purposive sampling method. The results obtained 24 genera phytoplankton of 3 classes. The abundance of phytoplankton ranges from 12.556-19.108 individu/m3. The distribution of phytoplankton is uneven and tends to follow the current direction. The phytoplankton Diversity Index indicated the values of 1,70â€“2,25 which were in the medium category. The phytoplankton evenness index indicated a value of 0,71 to 0,81 belonged to the High category. The dominance index in Morosari waters indicated no particular genus dominating with the values of 0,18-0,29. The Saprobik Index indicated the values of 0,6â€“1,3 which were into the category Î² â€“Mesosaprobic.

Pengaruh Konsentrasi KOH yang Berbeda Terhadap Kualitas Alginat Rumput Laut Coklat Sargassum duplicatum J. G. Agardh Anwar, Fauzi; Djunaedi, Ali; Santosa, Gunawan Widi
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Indonesia has plentiful resources and high biological diversity. One of them is brown seaweed Sargassum duplicatum which has potential as source for alginate. Alginate is often used in food and other industry, however extraction method is still becoming handycap to get high quality alginate. The objective of research was to know the effect of different concentration of soaking media KOH on quality of alginate. The method used was laboratory experiment. Design experiment applied was Completely Randomized Design (CRD) with three different concentrations of KOH, namely 0.3%, 0.5%, and 0.7%. Each treatment was repeated three times. The result showed that the highest yield was obtained at 0.3% KOH concentration at 33.63 Â± 2.11%. The highest viscosity was obtained at 0.7% KOH concentration at 23.33 Â± 2.08 cPs. The lowest water content was obtained at 0.7% KOH concentration at 14.71 Â± 0.32%. The ashes content was obtained at 0.7% KOH concentration at 27.82 Â± 0.88%.

Uji Aktivitas Antioksidan Ekstrak Kasar Keong Bakau (Telescopium telescopium) dengan Pelarut yang Berbeda terhadap Metode DPPH (Diphenyl Picril Hidrazil) Rahmayani, Ulfah; Pringgenies, Delianis; Djunaedi, Ali
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Mangrove snail (Telescopium telescopium) is one of the marine gastropod and some communities use as foodstuff. The purpose of study was to find out the antioxidant activity by crude extract of mangrove snails (T. telescopium) using DPPH method in different solvents. The method consists of extraction using gradient solvent (chloroform, ethyl acetate and methanol), phytochemical test and antioxidant activity test using DPPH method. DPPH test using a series of concentration of 100 ppm, 200 ppm, 400 ppm and 800 ppm with triplicate repetition. IC50 values were determined by calculating the regression analysis % inhibition against the concentration of crude extract. The crude extract of mangrove snails is contained three bioactive components in the form of alkaloids, steroids and flavonoids.The results showed that the IC50 value of chloroform, ethyl acetate and methanol extract were 47274.00 ppm, 4143.58 ppm and 2329.81 ppm, respectively. The IC50 values of all crude extract have a very weak antioxidant activity (IC50 > 200 ppm), with IC50 of BHT as positive control was 4.91 ppm.

Kandungan Total Lipid Chlorella Vulgaris Yang Dikultur Dalam Media Yang Diinjeksi CO2 Jawa, Ivend Umbu; Ridlo, Ali; Djunaedi, Ali
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Chlorella vulgaris biasa digunakan sebagai pakan alami dalam bidang budidaya. C.vulgaris sebagai produsen primer memerlukan CO2 dalam jumlah besar untuk proses fotosintesanya, sehingga berpotensi besar mampu menyerap dan mensintesis unsur karbon tersebut. CO2Â merupakan variabel pentingyangperludiperhatikan dalam budidayaC.vulgaris. Permasalahan yang timbul adalah CO2 tidak cukup disuplai melalui difusi sederhana dari udara karena konsentrasinya sangat rendah yaitu 0,03%. Tujuan dari penelitian ini adalah untuk mengetahuikandungan total lipidC.vulgaris yang dikultur dalam media yang di injeksi CO2. Perlakuan yang digunakan adalah injeksi CO2 selama 2 menit, 4 menit, 6 menit, 8 menit dan tanpa injeksi CO2 (kontrol). Kultur C.vulgarismenggunakan wadah erlenmeyer sebanyak 15 buah dengan volume 1 L yang diperkaya dengan pupuk Walne.Biota uji yang digunakan adalah C.vulgaris Â yang diperoleh dari koleksi kultur murni laboratorium mikroalga BBPBAP Jepara. Pengamatan yang diukur meliputi kandungan total lipid C.vulgaris, pH, CO2, suhu, salinitas, alkalinitas dan DO. C.vulgaris dipanen hari ke - 9 pada fase eksponensial akhir, selanjutnya dilakukan penimbangan biomassa dan dianalisis total lipidnya. Hasil penelitian menunjukan bahwa injeksi CO2 berpengaruh nyata (p<0,05)terhadap kadar total lipid C.vulgaris. Kadar total lipid tertinggi dihasilkan perlakuan 6 menit sebesar 63,47 %

Uji Pendahuluan Aktivitas Produk Biotransformasi Daun Mangrove Avicennia marina Dengan Isolat Jamur Terhadap Bakteri Patogen Klebsiella pneumonia dan Enterobacter aerogenes Sabiladiyni, Humairah Arifia; Trianto, Agus; Djunaedi, Ali
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

ABSTRAK : Penyebaran bakteri patogen Klebsiella pneumonia dan Enterobacter aerogenes di Indonesia pada tahun 2013 diketahui mencapai 67,81%. Sumber antibiotik berasal dari bahan kimia juga dari alam, salah satunya berasal dari jenis mangrove A. marina yang mengandung senyawa flavonoid, steroid, fenol, dan tannin. Ekstrak daun A. marina kurang efektif sehingga perlu dilakukan proses biotransformasi dengan harapan akan terbentuk senyawa baru yang lebih berpotensi. Penelitian ini dilakukan untuk mengetahui apakah ekstrak jamur dapat menghambat pertumbuhan bakteri dan penambahan daun mangrove pada media jamur akan mengubah senyawa yang terkandung pada ekstrak daun mangrove. Sampel daun mangrove Avicennia marina diambil di Desa Tugurejo, Semarang dan isolat jamur yang digunakan adalah Fusarium incarnatum, isolat C12 dan C14. Bakteri patogen yang akan diuji adalah bakteri patogen Klebsiella pneumonia dan Enterobacter aerogenes. Kultur jamur dilakukan dengan menambahkan 200 gr dan 400 gr daun mangrove dalam 1000 ml air laut sebagai campuran media. Aktivitas antibakteri dengan menggunakan metode difusi agar. Ekstraksi daun mangrove dan jamur simbion dengan pelarut metanol. Hasil ekstraksi kemudian diuji aktivitas antibakteri dengan konsentrasi 250 Î¼g/disk, 500 Î¼g/disk, dan 1000 Î¼g/disk. Deteksi kelas senyawa antibakteri menggunakan KLT. Visualisasi dengan sinar UV dan reagen vanillin asam sulfat. Ekstrak C14 dengan media daun mangrove 400 gr menunjukan zona hambat terbesar 12,3 mm pada konsentrasi 500 Î¼g/disk terhadap bakteri Enterobacter aerogenes dan termasuk golongan kuat. Hasil KLT menunjukkan ekstrak jamur C14 memiliki kandungan yang hampir sama dengan ekstrak mangrove, tetapi setelah disemprotkan vanillin asam sulfat terdapat senyawa lain yang berbeda dari ekstrak daun mangrove.Â ABSTRACT : The emerge of pathogenic bacteria Klebsiella pneumonia and Enterobacter aerogenes in Indonesia are now reached 67,81% in 2013. One of the natural source is mangrove A. marina that contained bioactive compound such as flavonoid, steroid, fenol,and tannin. Mangrove leaves extract is often not effective, so biotransformation process is necessary to change the previous substrate become the new potential compound. The aims of this research to know whether fungal extract can inhibit the bacterial growth and the addition of mangrove leaves on fungal media will change a compound contained on mangrove leaves extract. Mangrove leaves are collected from Desa Tugurejo, Semarang and fungal isolation using Fusarium incarnatum, C12 and C14. K. pneumonia and E. aerogenes were the pathogenic bacteria assayed. The addition of mangrove leaves around 200 gr and 400 gr for fungi culture. Antibacterial activity by agar diffusion method. Extraction of mangrove leaves and fungal symbiont were attached with methanol solvent. Then, this crude extract were assayed for antibacterial activity by the consecutive extract concentrations of 250, 500, and 1000 Î¼g/disk. Detection of class antibacterial compound was carried out using TLC method and visualization with UV light and vanillin sulfuric acid. The largest antibacterial activity in extract C14 about 12,3 mm with consentration 500 Î¼g/disk that inhibits Enterobacter aerogenes and belong to strong group. The results of TLC showed that extract C14 have almost the same compound with mangrove leaves extract, but after sprayed with vanillin sulfuric acid, there are several compound which different with mangrove leaves extract.

Co-Authors A. Santoso Adi Santoso ADI SANTOSO Adi Suryanto Adi Suryanto Agung Sudaryono Agus Sabdono Agus Trianto Ali Ridlo Ambariyanto Ambariyanto Amelia, Aninda Putri Anggelina, Amelia Cahya Anggit Puji Muswantoro Apriliani, Seka Indah Ayu Permana Sari Ayuning Smita Rukmi Bagus Pitra Aditya Bagus Pitra Aditya Bambang Sulardiono Bambang Yulianto Br Ginting, Feny Amelia Chrisna Adhi Suryono Delianis Pringgenies Delianis Pringgenies Dyah Rahmawati Endang Supriyantini Endarwantti, Vionita Ervia Yudiati Farizan Adiya Pratama, Farizan Adiya Fauzi Anwar Gunawan Widi Santosa Irwani Irwani Ivend Umbu Jawa Ivend Umbu Jawa, Ivend Umbu Jauharul Fadli Kharisma Firdaus Linggarjati Lestari, Raisha Fahmida Dwi Niâ€™amillah Niâ€™amillah Norma Afiati Nur Taufiq S.P.J. Panatar, Jakfar Shodiq Putri, Cinthya Ruhanto Raden Ario Rafsanjani, Muhammad Eka Darmawan Reza Hafiz Pranajaya Ria Azizah Tri Nuraini Richardus Mahatmada Indrajati Ristiyanto, Ardi Rizqina, Cahya Rudhi Pribadi Sabiladiyni, Humairah Arifia Safitri, Shofi Firda Sarjito - Sarjito . Setyati , Wilis Ari Shafiya, Radila Widya Siti Aminah Sri Redjeki Subagiyo Subagiyo Subagyo Subagyo Sunaryo Sunaryo Sunaryo Sunaryo Turnip, Sarah Pebriyani Ulfah Rahmayani W. Widiatmoko Wibowo, Ichsan Suryo Zaenal Arifin

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