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Journal : Natural B

Design of Cell Construction for Immunosensor Based Quartz Crystal Microbalance (QCM) Farida Wahyuni; Setyawan Purnomo Sakti; Unggul P. Juswono; Fenny Irawati; Nur Chabibah
Natural B, Journal of Health and Environmental Sciences Vol 1, No 4 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.764 KB) | DOI: 10.21776/ub.natural-b.2012.001.04.2

Abstract

The biosensor is a sensor device that combines biological compounds with a transducer. One type of biosensor that uses mass change detection techniques are QCM (Quartz Crystal Microbalance). QCM is a sensor that works with the principle of quartz crystal frequency shift due to mass deposition on the surface of the crystal. QCM can be used to detect the reaction between the molecules, so that the QCM can serve as biosensors that can be used for the diagnosis of a disease. In the development of QCM immunosensor for there are many problems, one of which is the construction of the cell. Construction cells can be used as a reaction between biomolecules. Construction of cells made of white Teflon. To keep QCM sensors are not experiencing physical stress that can lead to rupture due to pressure from the Teflon o-ring is used as an insulating silicon. The results of this study indicate that the construction of the cells created can be used as a medium for immobilization of Bovine Serum Albumin observations (BSA) on the surface of the sensor.
Determination of Optimum Anti Protein Specific Concentration of 208 kDa Milk Cow on QCM Sensor Nur Chabibah; Setyawan P. Sakti; Masdiana Ch. Padaga; Johan A. E. Noor; Fenny Irawati; Farida Wahyuni
Natural B, Journal of Health and Environmental Sciences Vol 2, No 3 (2014)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (488.922 KB) | DOI: 10.21776/ub.natural-b.2014.002.03.8

Abstract

The purpose of the research is to detect optimum concentration of immobilization of anti protein cow’s milk with 208 kDa molecular weight’s (anti PSS 208 kDa) by utilizing QCm (Quartz Crystal Microbalance) sensor. The purpose of research is to detect optimum concentration of immobilization of specific anti protein cow’s milk with 208 kDa molecular weight’s (anti PSS 208 kDa) by utilizing QCM (Quartz Crystal Microbalance) sensor. QCM sensor had been coated by polystiren solution of 5% which is heated until 200 ◦C. During measurement, QCM sensor place on data accuisition and QCM-Box measurement. 30 µL Anti PSS 208 kDa with concentration varied between 200 mg/L up to 1400 mg/L was dropped to QCM sensors. Frequency changes were recorded as a function of concentration of anti PSS 208 kDa. The frequency changes due to presence of anti PSS 208 kDa deposited on the surface of QCM sensor were not spontaneous, but takes time to reach steady state. As the concentration of anti PSS 208 kDa increased, so is the frequency change until it reaches saturation point at 1000 mg/L. The mass percentage of anti PSS 208 kDa deposited on QCM sensor surface is decreased with increasing anti PSS 208 kDa concentration. Therefore optimum immobilization concentration of anti PSS 208 kDa is define on frequency changes at first saturation point, that is at 1000 mg/L concentration namely 267 ± 5 Hz