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All Journal Jurnal Kedokteran Brawijaya Majalah Kedokteran Bandung Jurnal Kebidanan Midwiferia Jurnal Informasi Kesehatan Indonesia (JIKI) Journal of Issues in Midwifery Heart Science Journal
Pande Made Dwijayasa
Laboratorium Obstetri dan Ginekologi Rumah Sakit Umum dr. Saiful Anwar Malang
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Pengaruh Ekstrak Air Daun Kelor Terhadap Kadar Leptin dan Malondialdehyde Lemak Visceral Tikus Wistar yang Dipapar Depo Medroxyprogesterone Acetate Hastuti, Nur Aini Retno; Winarsih, Sri; Dwijayasa, Pande Made
Journal of Issues in Midwifery Vol 2, No 1 (2018)
Publisher : Journal of Issues in Midwifery

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (309.347 KB) | DOI: 10.21776/ub.JOIM.2018.002.01.4

Abstract

Penggunaan Depo Medroxyprogesterone dikaitkan dengan peningkatan adipogenesis dan oksidatif stress. Daun kelor (Moringa oleifera) adalah antioksidan alami dengan kandungan polifenol. Penelitian ini bertujuan untuk membuktikan ekstrak air daun kelor mempengaruhi kadar leptin dan malondialdehyde (MDA) lemak visceral tikus wistar yang dipapar Depo Medroxyprogesterone Acetate (DMPA). 25 tikus Wistar betina yang dibagi dalam kelompok kontrol negatif (0,2 ml aquades i.m dan 1 cc aquades/oral), kontrol positif (DMPA 2,7mg i.m dan 1 ccaquades/oral), perlakuan DMPA 2,7 mg dengan ekstrak air daun kelor 100 mg/kgBB, 150 mg/kgBB, dan 200 mg/kgBB. Penelitian dilakukan selama empat minggu dan dilakukan pengambilan jaringan lemak visceral. Kadar leptin diukur dengan metode ELISA dan kadar MDA dilakukan dengan metode TBARs menggunakan spektrofotometer. Tikus yang dipapar DMPA (kontrol positif) memiliki rerata kadar leptin dan MDA yang tertinggi dibanding dengan kelompok tikus yang tidak dipapar DMPA (kontrol negatif) ataupun tikus yang mendapat ekstrak air daun kelor (kelompok perlakuan). Uji ANOVA diperoleh p= 0,027  (leptin) dan p=0,001 (MDA) sehingga dapat diintepretasikan terdapat perbedaan yang signifikan pada kadar leptin dan MDA setelah pemberian ekstrak air daun kelor dalam berbagai dosis. Ekstrak air daun kelor menurunkan kadar leptin dan DMPA. Hasil uji Pearson Product Moment p=0,010 (leptin) dan p=0.000 (MDA). Hasil dari penelitian ini menyimpulkan ekstrak air daun kelor mampu menurunkan kadar leptin dan MDA lemak visceral tikus wistar yang dipapar Depo Medroxyprogesterone Acetate
Pengaruh Extra Virgin Olive Oil Terhadap Folikulogenesis dan Kadar Malondialdehyde Ovarium Pada Tikus yang dipapar Rhodamin B Rohmawati, Huda; Sujuti, Hidayat; Dwijayasa, Pande Made; Yudanto, Hendy Setyo
Jurnal Informasi Kesehatan Indonesia (JIKI) Vol 4 No 2 (2018): Jurnal Informasi Kesehatan Indonesia
Publisher : Politeknik Kesehatan Kemenkes Malang (State Health Polytechnic of Malang)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (531.243 KB) | DOI: 10.31290/jiki.v(4)i(2)y(2018).page:120-127

Abstract

Rhodamin B merupakan bahan pewarna tekstil disalahgunakan sebagai pewarna makanan. Rhodamin B bersifat toksik penyebab stres oksidatif, dan meningkatkan kadar MDA. Peningkatan kadar MDA menyebabkan gangguan folikulogenesis sehingga folikel tidak berkembang dan atresia. Hal tersebut berkontribusi pada kejadian infertil. Paparan rhodamin B berpengaruh pada folikulogenesis dan kadar MDA ovarium. Tujuan dari penelitian ini adalah membuktikan pengaruh EVOO pada Folikulogenesis dan kadar Malondialdehyde ovarium tikus betina galur wistar yang dipapar rhodamin B. Metode penelitian menggunakan True Experimental Post Test Only Control Group menggunakan 25 ekor tikus dibagi menjadi 2 kelompok kontrol dan 3 kelompok perlakuan dengan pemberian EVOO peroral dosis 1,5 ml/KgBB, 3 ml/KgBB, 4,5 ml/KgBB serta dipapar rhodamin B 18 mg/KgBB selama 36 hari. Kemudian dilakukan pengambilan ovarium untuk menghitung jumlah folikel dengan pewarnaan HE dan pengukuran kadar MDA dengan Spectrofotometri. Pemberian EVOO terhadap jumlah folikel primer, sekunder dan kadar MDA menunjukkan korelasi yang bermakna (p=0,000<α), dengan koefisien korelasi folikel primer 0,735, folikel sekunder 0,699, menunjukkan ada hubungan yang positif dan kuat. Pada kadar MDA didapatkan -0,808 sehingga ada hubungan yang negatif dan kuat. Kesimpulan: Pemberian EVOO dengan dosis 1,5 ml/KgBB, 3 ml/KgBB, 4,5 ml/KgBB berpengaruh terhadap folikulogenesis dan penurunan kadar MDA ovarium pada tikus betina yang dipapar rhodamin B.
Pengaruh Vitamin C dan E terhadap Histologi Tuba Fallopii pada Tikus yang Dipapar MSG Umami, Riza; Dwijayasa, Pande Made; Winarsih, Sri
Jurnal Kedokteran Brawijaya Vol 28, No 2 (2014)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2014.028.02.1

Abstract

Monosodium glutamat (MSG) adalah garam natrium dari asam glutamat yang digunakan sebagai penyedap rasa. MSG berpengaruh pada motilitas tuba yaitu pada lapisan otot polos dan jumlah sel epitel sekretorik tuba fallopii. Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi vitamin C dan E terhadap jumlah sel epitel sekretorik dan tebal lapisan otot polos tuba fallopii pada tikus yang dipapar MSG selama 42 hari. Desain penelitian yang digunakan adalah eksperimental laboratorik dengan menggunakan 25 ekor tikus betina dewasa yang dibagi menjadi 5 kelompok yaitu K(-) adalah kontrol negatif; K(+) MSG 0,7mg/g BB; PI mendapat MSG 0,7mg/gBB +vitamin C 0,2mg/gBB+vitamin E 0,04 IU/gBB; PII mendapat MSG 0,7mg/gBB +vitamin C 0,4mg/gBB+vitamin E 0,04 IU/gBB; PIII mendapat MSG 0,7mg/gBB+vitamin C 0,8mg/gBB+vitamin E 0,04 IU/gBB. Pada hari ke-43 tikus yang diterminasi adalah pada fase proestrus lalu dilakukan pemeriksaan histopatologi tuba fallopii dengan pengecatan HE yang diamati dibawah mikroskop Dot Slide. Uji one way ANOVA menunjukkan hasil bahwa kombinasi vitamin C dan E dapat meningkatkan jumlah sel epitel sekretorik dan tebal lapisan otot polos (masing-masing p=0,000 dan p=0,042). Diduga hal tersebut disebabkan oleh menurunnya kadar estrogen pada siklus reproduksi. Kesimpulan dalam penelitian ini adalah kombinasi dosis vitamin C dan vitamin E meningkatkan jumlah sel epitel sekretorik dan tebal lapisan otot polos tuba fallopii tikus yang dipapar MSG, meskipun tidak bermakna antar kelompok dosis.Kata Kunci: Jumlah sel epitel sekretorik, tebal lapisan otot polos, tuba fallopii, MSG, vitamin C, vitamin E
Pengaruh Susu Kedelai terhadap Ekspresi Reseptor Estrogen-Β Uterus pada Masa Reproduksi Awal Rattus Norvegicus Betina: Effect of Soy Milk on Uterine Estrogen-Β Receptor Expression in the Early Reproductive Period of Female Rattus Norvegicus Dinastiti, Vide Bahtera; Nurdiana, Nurdiana; Dwijayasa, Pande Made
Jurnal Kebidanan Midwiferia Vol. 5 No. 1 (2019): April
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/mid.v5i1.2619

Abstract

Receptors estrogen can also stimulated by other, namely by fitoestrogen. Fitoestrogen are the group of non steroid plants that have composition resembling the natural estrogen in the body. One of it is isoflavon, that can be binding with receptor estrogen, which affinity for RE-β is greater than RE-α and can make potential effect because receptors estrogen will blocked by isoflavon and cannot be occupied by estrogen.This compound can harm the endocrine system of the body because of the possible bad effects for reproductive function. Isoflavonoid is mostly found in soy. Soybean can be processed to soybean milk. The purpose of this study is demonstrate that administration of soy milk can affect the expression of RE-β uterus at the early reproductive period of female Rattus norvegicus. The method used is an experimental design post test control group design. The sample in this study is 24 Rattus norvegicus females. Then divided into four groups: one control group and three treatment groups were given soy milk each dose of 2.5 mg/kg/weight; 5mg/kg/weight; 10mg/kg/weight. The expression of RE-β uterus were measured by immunohistochemistry. Based on the analysis using ANOVA, was obtained p-value of 0.000, smaller than α = 0:05 (p <0.05). There is a significant influence on the administration of soy milk increase expression of RE-β and by LSD test obtained the results of differences from application of soy milk with different doses.
Differences in Elastin and Collagen Levels in the Levator Ani Muscle of Primiparous and Multiparous Normal Postpartum Women Rada, Annisa Astika; Rahajeng, Rahajeng; Dwijayasa, Pande Made; Gunawan, Didik Agus
Majalah Kedokteran Bandung Vol 56, No 4 (2024)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v56.3694

Abstract

The levator ani, comprising the pubococcygeus, puborectalis, and iliococcygeus muscles, is crucial for supporting pelvic organs, maintaining continence, and ensuring pelvic stability. Weaknesses in these muscles or ligaments can lead to pelvic organ prolapse (POP), a condition where the pelvic organs descend and protrude through the vaginal introitus. Collagen and elastin, the key constituents of the extracellular matrix in the levator ani muscle, play a significant role in maintaining its structural integrity and can be influenced by parity. This study aimed to determine the differences in elastin and collagen levels of the levator ani muscle of primiparous and multiparous patients. This was an observational analytical study on 18 postpartum female patients consisting of 9 primiparous and 9 multiparous in January-March 2023. Samples were levator ani muscle biopsies from perineal lacerations of at least grade II from the inpatient Obgyn Department of the Faculty of Medicine, Universitas Brawijaya. Examination was done using the immunohistochemical method. Results showed that the percentage of histological area secreting elastin and collagen was higher in the primiparous group than multiparous, thus the levels were higher (p<0.001; p=0.001; p<0.05). In conclusion, elastin and collagen levels were lower in multiparous women compared to primiparous women. Future studies can evaluate factors affecting the decline in elastin and collagen in multiparous women using a larger sample size.
Idiopathic Pulmonary Arterial Hypertension Newly Diagnosed in Pregnancy with Anemia and Threatened Preterm Labor Harumsari, Stefani; Rahardjo, Bambang; Prasetyorini, Nugrahanti; Dwijayasa, Pande Made; Yogibuana, Valerinna; Rohman, Mohammad Saifur
Heart Science Journal Vol. 3 No. 4 (2022): Prevention, Screening dan Rehabilitation : The Back Bone of Quality Care Improv
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.hsj.2022.003.04.7

Abstract

Background: Pulmonary hypertension (PH) is a rare cardiovascular disorder that leads to right heart failure (RHF). Although most PH occurs secondary to congenital heart disease (CHD), PH can occur primarily due to pulmonary arterial vasculature abnormalities, known as Idiopathic pulmonary arterial hypertension (IPAH). In addition, the physiologic changes during pregnancy can potentially lead to worsening PAH and confer a poor prognosis. Therefore, when the mother refuses termination, a multidisciplinary team should manage the pregnancy and delivery to improve maternal and fetal outcomes.Objectives: This case report aimed to describe the importance of early diagnosis and treatment in PAH.Case reports: We reported a case of a 24-year-old woman with idiopathic pulmonary arterial hypertension (IPAH) that was newly diagnosed at 25 weeks of pregnancy and previously misdiagnosed with patent ductus arteriosus (PDA). This pregnancy was complicated with anemia and threatened preterm labor. Sildenafil was used as a vasodilator to reduce the symptoms of PAH. Unfortunately, the pregnancy was terminated at 29 weeks because of PPROM after considering giving lung maturation and neuroprotectant to the fetus. The patient was discharged without complication, but the baby died after eight days of intensive care due to HMD II, which led to respiratory failure.Conclusion: PAH in pregnancy is a life-threatening condition if untreated. Continuous treatments can help control the symptoms and avoid further complications for both mother and baby.
Co-Authors A.A. Ketut Agung Cahyawan W Bambang Rahardjo Dinastiti, Vide Bahtera Gunawan, Didik Agus Harumsari, Stefani Hastuti, Nur Aini Retno Hidayat Sujuti Huda Rohmawati Mohammad Saifur Rohman Nina Mardiana (F01108057) Nugrahanti Prasetyorini Nurdiana Nurdiana Rada, Annisa Astika Rahajeng Rahajeng Sri Winarsih Yogibuana, Valerinna Yudanto, Hendy Setyo