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PENGHAMBATAN AKTIFASI NFκ κκ κB OLEH CAPE (CAFFEIC ACID PHENETHYL ESTER), KOMPONEN AKTIF MADU LEBAH (HONEYBEE HIVES),PADA HUVEC’S (HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS) YANG DIPAPAR LDL TEROKSIDASI Rohman, Mohammad Saifur; Rastini, Endah Kusuma; Sarbini, Dwi; A W, Titi; Widodo, Widodo; Sargowo, Djanggan
Jurnal Kedokteran Brawijaya Vol 22, No 1 (2006)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (948.806 KB) | DOI: 10.21776/ub.jkb.2006.022.01.1

Abstract

The high prevalence of cardiovascular morbidity and  mortality caused by complication of atherosclerosisneeds an optimal effort to prevent the atherosclerosis progression and complication. Atherogenesis is a chronicinflammatory process which could be prevented via the inhibitionof NF?B, a known key transcription factor involved in inflammatory process. An active component of honeybee hives, CAPE (Caffeic Acid Phenethyl Ester), is believed to inhibit inflammatory process via the inhibition of NF?B activation. However, it remains uncertain whetherCAPE inhibits NF?B activation in endothelial cells. This study therefore was aimed to examine the molecular mechanism of CAPE mediated NF?B inactivation in OxLDL-treated HUVEC?s. Immunohistochemistry using p50 antibody was applied to detectthe migration of NF?B (p50-p65 complex) from inactive form in cytoplasmto active form in nucleus.
ANALISIS URUTAN NUKLEOTIDA DAN EKSPRESI DARI MUSCLE A-KINASE ANCHORING PROTEIN (mAKAP) MENUNJUKKAN KEMUNGKINAN FUNGSI mAKAP PADA DIFERENSIASI KARDIMIOSIT: PERBANDINGAN EKSPRESI mAKAP PADA JANTUNG MDX DAN KONTROL Rohman, Mohammad Saifur
Jurnal Kedokteran Brawijaya Vol 20, No 1 (2004)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.174 KB) | DOI: 10.21776/ub.jkb.2004.020.01.1

Abstract

Muscle A-Kinase Anchoring Protein (mAKAP) is an A-kinase anchoring protein (AKAP) which targets cAMP-dependent protein kinase(PKA) to the nuclear envelope. mAKAP not only binds to PKA, but also to the ryanodine receptor (RyR2) and the rolipram-inhibitedcAMP-specific phosphodiesterase (PDE4D3). Amino acid sequence analysis revealed that mAKAP possesses LXCXE andFYDYSYL, the consensus-binding domain of pRB and CBP/p300, respectively. pRB and CBP/p300 are known as a key componentsin cardiomyocyte differentiation processes. Northern blot analysis revealed that mAKAP was expressed in a 13 day old rat heart andits expression increased by 15 days of age when cardiomyocytes reveal a terminal differentiation phenotype. In culturedcardiomyocytes mAKAP was expressed in differentiated but not undifferentiated. Accordingly, mAKAP may play a role in the terminaldifferentiation process through pRB-CBP/p300 functions. Furthermore, we observed mAKAP expression in old mdx heart, a mousemodel of Duchenne muscular dystrophy, compared to control mice. In the control heart, mAKAP transcripts were detected at 6-, 20-,64- and 76-weeks of age. However, mAKAP expression significantly appeared only in 64- and 76-week old mdx hearts. DelayedmAKAP expression in mdx may contribute to impaired function of pRB, CBP/p300, cAMP and Ca2+ complex.Key words: mAKAP, domain architecture, terminal differentiation, pRB, CBP/p300, mdx.
Development of an Experimental Model of Metabolic Syndrome in Sprague Dawley Rat Mohammad Saifur Rohman; Mifetika Lukitasari; Dwi Adi Nugroho; Widodo Nashi; Nur Ida Panca Nugraheini; Teguh Wahyu Sardjono
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (739.041 KB) | DOI: 10.21776/ub.rjls.2017.004.01.10

Abstract

The study was designed to establish an experimental models of metabolic syndrome that adequately mimic metabolic syndrome criteria as determined by NCEP ATP III.Eighteen Male Sprague dawley rats, 2 - 3 months old were used in the study. Combination of high fat and high sucrose (HFHS) diet for eight weeks and streptozotocin (STZ) injection in the second week was administered to induce metabolic syndrome. The body weight and biochemical parameters (blood glucose, triglyceride, HDL-cholesterol) were measured every 2 weeks. The rats with blood glucose (>126mg/dL), triglyceride (TG) (>150mg/dL), high systolic blood pressure (≥140 mmHg), and reduced HDL levels (<40 mg/dL) confirmed presence NCEP-ATP III criteria of metabolic syndrome. The adiponectin level was analyzed by ELISA methods.Fasting blood glucose, triglyceride, and systolic blood pressure increased significantly (p<0.05) in HFHS group compared to that of NC group. Moreover, after 8 week a significant lower HDL level was observed in HFHS group compared to that of NC group. In addition, HFHS group showed a significantly lower adiponectin level compared to that of NC group.The combination of low doses of STZ (30mg/kg) and HFHS administration for 8 weeks could induce metabolic syndrome mimicking human criteria of metabolic syndrome.
Physalis minima Leaves Extract Induces Re-Endothelialization in Deoxycorticosterone Acetate-Salt-Induced Endothelial Dysfunction in Rats Dian Nugrahenny; Nur Permatasari; Mohammad Saifur Rohman
Research Journal of Life Science Vol 4, No 3 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (484.898 KB) | DOI: 10.21776/ub.rjls.2017.004.03.6

Abstract

The administration of deoxy-corticosterone acetate (DOCA)-salt can induce oxidative stress leading to decrease the bioavailability of nitric oxide (NO), increase senescence of circulating endothelial progenitor cells (EPCs), thus contributing to endothelial dysfunction. This study was aimed to investigate the effects of Physalis minima L. leaves extract on serum NO levels, circulating EPCs number, and histopathology of tail artery endothelial cells in DOCA-salt-induced endothelial dysfunction in rats. Twenty-five male Wistar rats were randomly divided into five groups: rats without any treatment (normal), rats treated with DOCA (10 mg/kgBW s.c. twice weekly) and given 0.9% NaCl to drink ad libitum for 6 weeks, and DOCA-salt-induced rats orally supplemented with P. minima leaves extract at doses of 500, 1500, or 2500 mg/kgBW for 4 weeks. Serum NO levels were measured by colorimetry. The number of circulating EPCs (CD34+/CD133+ cells) was determined by flow cytometry. The tail artery sections were histologically processed with hematoxylin-eosin staining. DOCA-salt-induced rats showed significantly (p<0.05) decrease in serum NO levels and circulating EPCs number compared to the normal. There was also more detached tail artery endothelial cells in DOCA-salt-induced rats. P. minima leaves extract at a dose of 500 mg/kgBW significantly (p<0.05) increased serum NO level and circulating EPCs number, and also induced an optimal re-endothelialization in DOCA-salt-induced rats. P. minima leave extract dose-dependently increases NO bioavailability contributing to enhanced EPCs mobilization, thereby promoting re-endothelialization in DOCA-salt-induced endothelial dysfunction in rats.
Patogenesis dan Terapi Sindroma Metabolik Mohammad Saifur Rohman
Jurnal Kardiologi Indonesia Vol. 28, No. 2 Maret 2007
Publisher : The Indonesian Heart Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30701/ijc.v28i2.261

Abstract

Arus globalisasi tidak hanya membawa dampak positif di segala bidang seperti informasi dan teknologi, namun sangat berpengaruh pada pola hidup terutama pola aktivitas dan makan. Produk dari teknologi dan informasi sebagian telah terbukti bermanfaat untuk mempermudah manusia (tidak banyak keluar tenaga), seperti mobil dan sarana transportasi lainnya, eleva-tor/lift, remote kontrol, traktor dan teknologi pertanian lainnya. Namun disisi lain, kemudahan-kemudahan tersebut mengakibatkan pola hidup sed-entary. Disamping itu, makanan tinggi kalori dan cepat saji kini mudah didapat di setiap tempat, amat membantu disela kegiatan rutin yang padat. Dengan demikian terciptalah asupan kalori yang tinggi dengan pemakaian energi yang rendah, lalu sisanya tersimpan dalam bentuk lemak. Sehingga mudah dipahami bahwa, saat ini sudah terjadi epidemi global overweight dan obesitas.
Kulit Manggis Menghambat Aktivasi Nuclear Factor ?B (NF-?B) dan Menurunkan Ekspresi ICAM-1 pada Tikus dengan Diet Tinggi Kolesterol H Hendarto; Mohammad Saifur Rohman; Djanggan Sargowo
Jurnal Kardiologi Indonesia Vol. 34, No. 1 Januari - Maret 2013
Publisher : The Indonesian Heart Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30701/ijc.v34i1.302

Abstract

Background: Atherosclerosis is widely viewed as an inflammatory disease with hypercholesterolemia being a dominant underlying risk factor. This study aimed to determine the effect of mangosteen pericarp in inhibition of NF-?B activation and ICAM-1 expression in rat fed with high cholesterol.Methods and Results: Various doses of crude extract mangosteen pericarp were administered to the high fat diet wistar rats and the activity of NF-?B measured by immunohistochemistry to assess nuclear NF-?B expression and the ICAM-1 expression. The high fat diet resulted significant increased serum LDL levels. Increased nuclear NF- ?B activation and ICAM-1 expression were also observed in high fat diet rats in concurrence with increased serum LDL. The inhibitory effect on NF- ?B activity and ICAM-1 expression was observed when 400 mg of mangosteen pericarp crude extract was administered and even showed a higher inhibitory effect in 800 mg of mangosteen pericap treated rats. The 800 mg extract treatment resulted in decreased ICAM-1 expression similar to those of non high fat rats.Conclusion: The administration of 800 mg mangosteen pericarp crude extract significantly inhibited NF-?B activation and reversed the expression of ICAM -1 to the normal level in high cholesterol diet rats.
Kadar Gula Darah Lebih Tinggi pada Pasien Hipertensi dengan Delesi Intron 16 Gen Ace Lowry Yunita; Mohammad Saifur Rohman; Bagus H Kuncahyo; Mifetika Lukitasari; Arina Madjidi; Maulidiyatun Nuril Faizah; Nashi Widodo
Jurnal Kardiologi Indonesia Vol. 37, No. 1 Januari - Maret 2016
Publisher : The Indonesian Heart Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30701/ijc.v37i1.551

Abstract

Background Renin Angiotensin Aldosteron System plays major role in hypertension pathophysiology. Has been proposed that half of individuals with essential hypertension are considered insulin resistant. Angiotensin Converting Enzyme gene polymorphism taking the control of ACE level in human plasma. There might be a correlation between ACE gene polymorphism and plasma glucose level.Objective This study sought to investigate ACE gene polymorphism insertion/deletion in hypertensive patients and its correlation with plasma glucose level.Method A total of 100 hypertensive patients at Saiful Anwar cardiovascular outpatients clinic who received ACEi at least 8 weeks were included. ACE genotype were determined by polymerase chain reaction method, and plasma glucose level was measured by enzymatic colorimetric assay method.Result The II, ID, and DD genotype were observed in 48%, 29% and 23% of participant, respectively. The fasting and 2 hours post prandial plasma glucose were significantly higher in DD genotype patients. The insertion/deletion polymorphisms were not related significantly to age, sex, cough, and controlled blood pressure of those patients.Conclusion This study showed that 23% of hypertensive patients had DD genotype with higher plasma glucose level as compare to those of II and ID genotype.
Hemodynamic Congestion at Hospital Discharge Predicts Rehospitalization during Short Term Follow Up in Acute Heart Failure Patients Yoga Waranugraha; Mohammad Saifur Rohman; Setyasih Anjarwani
Jurnal Kardiologi Indonesia Vol 40 No 3 (2019): Indonesian Journal of Cardiology: July-September 2019
Publisher : The Indonesian Heart Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30701/ijc.v40i3.805

Abstract

Background: Hemodynamic congestion is an increase in left ventricular diastolic pressure (LVEDP) without clinical symptoms and signs of congestion. Current acute heart failure (AHF) treatment goals only focused on improving clinical congestion. The purpose of this study was to investigate whether hemodynamic congestion measured by NT-proBNP level and ePCWP at hospital discharge could predict short term clinical outcomes in AHF patients. Method: This prospective cohort study was conducted at dr. Saiful Anwar General Hospital Malang from January to July 2018. All patients got AHF treatment according to the 2016 ESC guidelines for heart failure. All patients were discharged without symptoms and signs of clinical congestion. Hemodynamic congestion at hospital discharge was defined as failure of treatment during hospitalization to achieve a reduction in NT-proBNP level >30% and/or ePCWP at hospital discharge >16 mmHg. NT-proBNP level and ePCWP were measured at 0-12 hours after hospital admisssion and at hospital discharge. ePCWP was measured using echocardiography. The clinical outcomes assessed were AHF rehospitalization and cardiovascular mortality within 30 days after hospitral discharge. Subgroup analysis was performed to determine therapeutic regimens that are effective in improving hemodynamic congestion. Result: A total of 33 AHF patients were included in this study. 48% patients were discharged with hemodynamic congestion and 52% patients discharged without hemodynamic congestion. Patients with hemodynamic congestion at hospital discharge showed a higher rehospitalization within 30 days (8 [50%] vs 1 [5.9%]; P = 0.007). Mortality within 30 days in both groups did not show a significant difference (2 [12.5%] vs 0 [0%]; P = 0.277). Treatment regiment of optimal dose of ACEi/ARB, β-blockers, and diuretics was associated with improvement of hemodynamic congestion (P = 0.026; r = 0.454), a decrease in NT-proBNP> 66% (P = 0.02; r = 0,574), and achievement of ePCWP <16 (P = 0,013; r = 0,493) at hospital discharge in HFrEF patients. Conclusion: This study showed that hemodynamic congestion assessed with NT-proBNP level and ePCWP at hospital discharge increased 30 day rehospitalization in AHF patients. In HFrEF, improvements in hemodynamic congestion can be achieved by giving the treatment regiment of optimal dose of ACEi/ARB, β-blockers, and diuretics. Keyword: Acute heart failure, hemodynamic congestion, NT-proBNP, ePCWP
The Effect of Bisoprolol on Heart Failure Preserved Ejection Fraction/HFpEF Patient’s Quality of Life Ayu Asri Devi Adityawati; Anna Fuji Rahimah; Mohammad Saifur Rohman; Setyasih Anjarwani; Djanggan Sargowo
Heart Science Journal Vol 2, No 4 (2021): Management of Thrombosis in Covid-19 Patient with Cardiovascular Disease
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.hsj.2021.002.04.6

Abstract

Global Publich Health Burden of Heart Failure reported the growing prevalence of heart failure from 26 million people affected in 2017 to 64.3 million affected in 2020 worldwide with half of the case classified as heart failure preserved ejection fraction/HFpEF. It is well known that someone who have been diagnosed with heart failure will have a poorer quality of life/QoL. β-blocker is a heart rate lowering agent with a potency to improve the patient’s clinical outcome. One clinical outcome that should always be evaluated and become the main goal of the therapy is QoL of the patient. This study aimed to observe and evaluate the effect of Bisoprolol, a type of β-blocker, in the improvement of HFpEF patient’s QoL. This study is a retrospective cohort following HFpEF patients who received β-blocker and HFpEF patients who did not received β-blocker in a span of 6 months. The study participant selected by purposive sampling method. Our study found that from all HFpEF patients who received β-blocker 86 patients had a good QoL, 16 patients had a Mod QoL, and 2 patients had a poor QoL (p=0.000). Mean physical score in the patient who did not received β-blocker was 13.84±11.01 while the mean score of the patient who received β-blocker was 5.94±4.95 (p=0.000). Mean emotional score in the patient who did not received β-blocker was 6.02±6.61 while the mean score of patient who received β-blocker was 1.83±2.16 (p=0.000). We conclude that the use of Bisoprolol could improve the HFpEF patient’s QoL evaluated by total score, physical score, and emotional score.   
Correlation of Ischemic Time with Diastolic Dysfunction and Correlation of Diastolic Dysfunction with 6 Minute Walk Test Distance in STEMI Patients Receiving Percutaneus Coronary Intervention Imelda Krisnasari; Anna Fuji Rahimah; Mohammad Saifur Rohman; Setyasih Anjarwani; Indra Prasetya
Heart Science Journal Vol 3, No 2 (2022): Improving ST-Elevation Myocardial Infraction Patients Prognosis
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.hsj.2022.003.02.5

Abstract

Background: Minimizing the time between ischemia and reperfusion in STEMI patients is critical for salvaging ischemic myocardium and limiting residual injury. One of the methods for determining the impact of ischemic time on the myocardium and correlating the findings to the outcomes is by using echocardiography.Objectives: To evaluate the correlation of ischemic time to diastolic dysfunction and also the correlation of diastolic dysfunction to functional capacity by 6-minute walk distance in STEMI patients. Methods: The study was a retrospective cohort, with all STEMI patients who underwent PCI at RSUD Dr. Saiful Anwar Malang between January 2018 and November 2021 being eligible. All patients underwent PCI, echocardiography, and a 6-minute walk test before being discharged. We defined significant diastolic dysfunction as grade 2 or 3 diastolic dysfunction, according to the 2016 American Society of Echocardiography (ASE) and European Association of Cardiovascular Imaging (EACVI) guidelines. The distance of 6-minute walk test was divided into three categories: less than 300m, 300-400m, and more than 400m.Results: From total 258 patients, 92 patients (35.7%) had significant diastolic dysfunction. The significant diastolic dysfunction was correlated with ischemic time > 12 hours (r = 0.51, p = 0.000), Killip class (r= 0.46, p = 0.000), culprit artery (r= 0.203, p=0,001), and peak toponin I levels (r=0.35, p=0.000). We identified that the ischemic time (odds ratio / OR 6.78; 95% Confidence Interval / CI 3.27 – 14.09; p  = 0.000), Killip class (odds ratio 4.62, 95% CI 2.53 to 8.48, p = 0.000), and infarct size by peak troponin I levels (OR 1.12, 95% CI 1.06 to 1.18, p  = 0.000) as independent predictors of significant diastolic dysfunction. There was inversely correlation of diastolic dysfunction with 6-minute walk test distance. (r = -0,422 dan p=0,000), with E/e’ as independent predictors of level of 6 minute walk distance (OR -1.126, 95% CI -1.78 to -0.48, p = 0.001).Conclusion: Ischemic time is one of the independent predictors of significant diastolic dysfunction. LV diastolic dysfunction was inversely correlated with 6-minute walk test distance in STEMI patient.
Co-Authors Adhika Prastya Wikananda Adi, Andi Wahjono Aditha Satria Maulana Alfata, Fandy Hazzy Anita Surya Santoso Anjarwani, Setyasih Anna Fuji Rahimah Anna Fuji Rahimah Anna Fuji Rahimah Anna Fuji Rahimah Anna Fuji Rahimah Anna Fuji Rahimah Anna Fuji Rahimah Anna Fuji Rahimah Annisa Hasanah Ardhani Galih Prakoso Ardhino, Ardhino Ardian Rizal Ardian Rizal Ardian Rizal Arief Wibisono Arif Wicaksono Arina Madjidi Ashari, Yordan Wicaksono Astiawati, Tri Aulanni'am Aulanni'am Ayu Asri Devi Ayu Asri Devi Adityawati Aziz, Indra Jabbar Bagus H Kuncahyo Bahar, Mokhamad Aswin Bambang Kusbandono Bambang Rahardjo Bayu Aji Bayu Lestari Budi Satrijo Budi Satrijo Budi Satrijo Caesario, Fahreza Cholid Tri Tjahjono Cholid Tri Tjahjono Cholid Tri Tjahjono Candra Citra Tarannita Dadang Hendrawan Dea Arie Kurniawan Dedy Irawan Dhani, Fauzan Kurniawan Dian Nugrahenny Djanggan Sargowo Djanggan Sargowo Djanggan Sargowo Djanggan Sargowo Djanggan Sargowo Djanggan Sargowo Djanggan Sargowo Dwi Adi Nugroho Dwi Adi Nugroho Dwi Adi Nugroho Dwi Sarbini Dwigustiningrum, Nur Kaputrin Endah Kusuma Rastini Evit Ruspiono Fahmi Rusnanta Fahmy Rusnanta Fahmy Rusnanta Fajar, Jonny Karunia Fandy Hazzy Alfatta Faris Wahyu Nugroho Firdaus, Achmad Jauhar Fitranti Suciati Laitupa Ghazyarda Aqilah Setya H Hendarto Harumsari, Stefani Haryati, Lina Hendrawati Hendrawati Heny Martini Hidayat Sujuti Hikmawan Wahyu Sulistomo Husnul Khotimah Ika Setyo Rini Ikhwan Handirosiyanto Imelda Krisnasari Imelda Krisnasari Indra Prasetya Indra Prasetya Indra Prasetya Inggita Kusumastuty Irma Kamelia Pratiwi Kahadi, Cik Krishna Ari Nugraha Kurnianingsih, Novi Laitupa, Fitranti Suciati Lenny Kartika Lestari, Puspa Liemena Harold Adrian Lowry Yunita Martini, Heny Maulidiyatun Nuril Faizah Mifetika Lukitasari Mifetika Lukitasari Mifetika Lukitasari Mifetika Lukitasari Millisani, Hayla Iqda Mohammad Aris Widodo Muchammad Dzikrul Haq Karimullah Muhamad Bayu Aji Muhammad Rizki Fadlan Muhammad Rizki Fadlan Muhammad Rizki Fadlan Nashi Widodo Nashi Widodo Noverike, Nikhen Novi Kurnianingsih Novi Kurnianingsih Novi Kurnianingsih Nugraha, Krishna Ari Nugraha, Yudha Tria Nugrahanti Prasetyorini Nur Ida Panca Nugraheini Nur Ida Panca Nugrahini Nur Permatasari Oktafin Srywati Pamuna Olivia Handayani Olivia Handayani Pamuna, Oktafin Srywati Pande Made Dwijayasa Pawik Supriadi Pawik Supriadi Putri Annisa Kamila Putri Annisa Kamila Putri, Valerinna Yogibuana Swastika Rahimah, Anna Fuji Rahmi, Koyuki Atifa Ratih Kusuma Wardani Ratna Pancasari Ratna Pancasari Renny Suwarniaty Rislan Faiz Muhammad Rizal , Ardian Rizal, Ardian Rochmawati, Icmi Dian Salvatore Di Somma Sasmojo Widito Satrijo, Budi Seprian Widasmara Setiawan, Dion Setyasih Anjarwani Setyasih Anjarwani Setyowati, Danti Utami Suprayoga, Imam Mi'raj Suryanto Taufieq Ridlo Makhmud Taufiq Nur Budaya Teguh Aryanugraha Teguh Wahyu Sardjono Titi A W Tonny Adriyanto Veny Kurniawati Veny Kurniawati Wella Karolina Widito, Sasmojo Widodo Nashi Widodo Widodo Wikananda, Adhika Prastya Wira Kimahesa Anggoro Wira Kimahesa Anggoro Yoga Waranugraha Yoga Waranugraha Yoga Waranugraha Yogibuana, Valerinna Yoseph Budi Utomo Yusuf Arifin