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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Veteriner Microbiology Indonesia The Journal of Experimental Life Sciences (JELS) Journal of Innovation and Applied Technology Jurnal Biodjati JFMR (Journal of Fisheries and Marine Research) Berkala Penelitian Hayati
Yoga Dwi Jatmiko
Department Of Biology Faculty Of Mathematics And Natural Sciences Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Veterinary

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Characterization of Probiotics Isolated from Intestine of Mackerel Fish (Rastrelliger sp.) from Lembata Regency of East Nusa Tenggara Helena Daten; Tri Ardyati; Yoga Dwi Jatmiko
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.04

Abstract

The research aimed to isolate, characterize, and analyze the ability of lactic acid bacteria (LAB) potential as probiotics to produce hydrolase enzyme. The LAB was isolated using MRS agar by the spread plate method. The LAB characterization includes antimicrobial activity, tolerance to low pH, bile salt, salinity, autoaggregation properties, and ability to produce hydrolytic enzymes. The isolate which has the highest ability to inhibit Aeromonas hydrophila is KBP 3.3, while the isolate which inhibits the highest Streptococcus agalactiae is KBP 1.1.1. The KBP 3.3 and KBP 1.1.1 were able to survive at pH 1 for 24 hours with a survival rate of 93.6% and 98.3%. The KBP 3.3 and KBP 1.1.1 are tolerant to 7.5% bile salt concentrations for 24 hours of 99.46% and 99.11%. The KBP 3.3 is tolerant to 0.5 % salinity for 24 hours with the highest survival rate of 113.38%, while KBP 1.1.1 is 94%. The KBP 3.3 and 1.1.1 have autoaggregation properties of 92.18% and 87.84%. The KBP 3.3 produced the highest lipase enzyme, while KBP 1.1.1 produced the protease enzyme.Keywords: hydrolytic enzyme, lactic acid bacteria, mackerel, probiotic
Antimicrobial Activity of Combination Bacteriocin and Asam Sunti Extract (Averrhoa bilimbi L. fermented) Against Multidrug Resistant Escherichia coli in Lettuces (Lactuca sativa) Angie Via Resty Kimbal; Yoga Dwi Jatmiko; Tri Ardyati
The Journal of Experimental Life Science Vol. 11 No. 2 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.02.06

Abstract

The ready-to-eat vegetables are often associated with the presence of multidrug-resistant (MDR) bacteria. This study aimed to evaluate the potency of bacteriocin, Asam Sunti extract, and their combination against MDR E. coli in lettuce. Their antimicrobial activity was assessed using the disk diffusion method and bacterial enumeration after direct application in pre-inoculated lettuce with MDR E. coli. The bacteriocin was produced by Lactobacillus plantarum BP102 at optimum production time or during the stationary phase at 18 h. These bacteriocins were able to inhibit five MDR E. coli isolates, while Asam Sunti extract and the combination of bacteriocin and Asam Sunti extract were only able to inhibit three MDR E. coli (LL1.2, LL3.11, and LL3.12) and (LL1.2, LL1.3, and LL3.11), respectively. In direct application to pre-inoculated fresh lettuce, higher inhibition of MDR E. coli was observed after applying the combination of bacteriocin and Asam Sunti extract with a ratio of 1:1 and 1:2, compared to bacteriocin alone. However, the inhibitory activity of this combination treatment was not significantly different (p>0.05) with the Asam Sunti extract alone. The highest rate of decrease in total bacteria in lettuces was 97% occurred in isolate LL1.2 with bacteriocin treatment alone, and isolate LL3.11 with combination treatment of bacteriocin and Asam Sunti extract (1:2). While on MCA media, the best reduction rate of 94% occurred in isolate LL1.2 with treatment using bacteriocin only, Asam Sunti extract only, and their combination (1:2). The inhibition of MDR E. coli in fresh lettuces by bacteriocin, Asam Sunti extract, and their combination was strain-dependent which was indicated by various inhibition results in all treatments.Key words: Asam Sunti extract, Bacteriocin, multidrug resistant.
Detection of Plantaricin-Encoding Gene and Its Partial Purification in Lactobacillus plantarum BP102 Elsa Mega Suryani; Yoga Dwi Jatmiko; Irfan Mustafa
Jurnal Biodjati Vol 8, No 2 (2023): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i2.27851

Abstract

Lactobacillus plantarum BP102 isolated from garlic bulb tissue has probiotic properties, including producing bacteriocin called plantaricin. This study aimed to detect the gene encoding bacteriocin produced by Lactobacillus plantarum BP102, and to evaluate the bacteriocin activity at each stage of partial purification. After the end of the log phase of L. plantarum BP102 was determined, and the bacteriocin-encoding genes were checked by PCR technique. Partial purification of bacteriocin was elucidated including pH-neutralized cell-free-supernatant (CFS), precipitation using 80% of ammonium sulfate, and dialysis (cut-off 10 kDa), then the bacteriocin activity in every partial purification stage was evaluated. The molecular weight of plantaricin was estimated using SDS-PAGE analysis. Lactobacillus plantarum BP102 harbored the gene encoding plantaricin (pln) biosynthesis, namely plnEF and plnK genes. The activity of crude bacteriocin was inactivated by the presence of proteinase-K enzyme. The protein concentration was gradually decreased along with the purification process. The bacteriocin activity was demonstrated at each step of the purification process (CFS, precipitation, and dialysis) against Bacillus cereus by 9.23 ± 0.20 mm, 7.86 ± 0.15 mm, and 7.6 ± 0.10 mm, respectively; while, Escherichia coli by 10.3 ± 0.55 mm, 7.4 ± 0.1 mm, and 6.86 ± 0.45, respectively. The molecular weight of partially purified bacteriocin BP102 was found to be approximately 15.9 kDa. The overlaid part of the gel showed a slight inhibition against E. coli due to a low protein concentration. This bacteriocin purification process should be further optimized to improve the bacteriocin activity that could be useful for food preservation.
Profil Resistansi Antibiotik dari Enterobacteriaceae yang Diisolasi dari Kucing (Felis catus) Furzania Mumtaza; Yoga Dwi Jatmiko; Herjuno Ari Nugroho; Sugiyono Saputra
Jurnal Veteriner Vol 24 No 3 (2023)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2023.24.3.335

Abstract

Kucing (Felis catus) merupakan hewan peliharaan populer yang memiliki interaksi dekat dengan manusia, sehingga membuka peluang penularan patogen. Penelitian ini bertujuan untuk mengidentifikasi keberadaan bakteri indikator dan menyelidiki profil resistansi antimikrob (antimicrobial resistance) (AMR). Tujuh sampel ulas/swab rektum diambil dari kucing dengan menggunakan amies-collecting swab. Uji presumtif Escherichia coli menunjukkan tujuh isolat (87,5%) teridentifikasi sebagai E. coli, yang ditunjukkan dengan koloni berwarna hitam dengan pendaran hijau-metalik pada eosin methylene blue agar (EMBA). Sebanyak delapan koloni bakteri berhasil diisolasi dan diuji resistansinya menggunakan metode difusi cakram. Tiga isolat resistan terhadap sefalosporin generasi ketiga (ceftriaxone), yang mengindikasikan bahwa kucing berpotensi menjadi sumber AMR. Berdasarkan analisis gen 16S rRNA dan konstruksi pohon filogenetik pada isolat terpilih, enam isolat diidentifikasi sebagai E. coli (S2, S4, S6, S10, dan KR6) dan satu isolat lainnya sebagai Enterobacter cloacae (S8). Penelitian ini menunjukkan bahwa kucing berpotensi sebagai pembawa bakteri yang resistan terhadap antibiotik.
Co-Authors Abdul Aziz Jaziri, Abdul Aziz Aditya Ragil Suharto Affan Gaffar Agung Putra Purwanto Alifia Issabella Mulyawati Angie Via Resty Kimbal Asep Awaludin Prihanto Dayu Nirwana Putri Eko Widodo Elsa Mega Suryani Endang Arisoesilaningsih Eufrasia Reneilda Arianti Lengur Furzania Mumtaza Helena Daten Herjuno Ari Nugroho Hidayatun Muyasyaroh Irfan Mustafa MARY D BARTON MIGUEL DE BARROS LOPES Rahmi Nurdiani Siska Aditya Sri Widyarti Sugiyono Saputra Tri Ardyati Wiwik Handayani