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All Journal International Journal of Biosciences and Biotechnology Jurnal Ilmu Dasar IPTEK Journal of Proceedings Series BERKALA SAINSTEK Biogenesis: Jurnal Ilmiah Biologi Biotropika Pelita Perkebunan Journal of Mathematical and Fundamental Sciences Jurnal Biodjati BIOSFER : Jurnal Biologi dan Pendidikan Biologi JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Journal of Natural Sciences
Kahar Muzakhar
Department Of Biology, Faculty Of Mathematics And Natural Science, University Of Jember, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Mathematics Physics Public Health Other

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Preferensi Oviposisi Hypothenemus hampei Ferr. Coleoptera:Scolitidae) terhadap Beberapa Jenis Biji Kopi Purwatiningsih, Purwatiningsih; Rosyanda Fisel Kosaya; Kahar Muzakhar
Biosfer : Jurnal Biologi dan Pendidikan Biologi Vol. 10 No. 1 (2025): BIOSFER: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP Unpas,

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23969/biosfer.v10i1.23546

Abstract

Kemampuan serangga Hypothenemus hampei dalam menentukan tempat bertelur dan pemilihan makan adalah hal yang sangat penting untuk kesuksesan hidup dan keturunannya. Setelah menemukan tanaman, selanjutnya serangga melakukan penafsiran terhadap kesesuaian tanaman sebagai inang. Penafsiran serangga ini tidak terlepas dari kombinasi faktor visual dan kimia. Oleh karena itu dilakukan penelitian terkait preferensi oviposisi H. hampei pada beberapa jenis biji kopi yaitu Coffea robusta, C. arabika dan C. excelsa. Tujuan penelitian ini adalah untuk mengetahui jenis biji kopi manakah yang lebih disukai H. hampei untuk meletakkan telurnya. Penelitian ini merupakan  penelitian eksperimental yang menggunakan Rancangan Acak Lengkap (RAL) dengan faktor tunggal yang terdiri dari 3 jenis biji kopi perlakuan dengan masing -masing 10 ulangan setiap perlakuan yaitu A (biji kopi Arabika), R (biji kopi Robusta) Dan E (biji kopi Excelsa). Uji penelitian dilakukan dengan metode choice dan  nonchoice. Penelitian ini menggunakan 10 serangga betina H. hampei. Pengamatan jumlah telur dilakukan pada hari ke-tujuh. Analisis data menggunakan uji analisis statistik  ANOVA (Analysis of Varian) (α  = 5%) dan uji lanjut Uji LSD (α  = 95%)  dengan menggunakan program SPSS 16.0 for Windows Evaluation Version. Hasil analisis  varian  menunjukkan bahwa preferensi oviposisi pada tiga jenis biji kopi terdapat perbedaan. Preferensi oviposisi terbesar ditunjukkan pada biji kopi robusta (C. robusta) yaitu dengan jumlah presentase preferensi sebesar {96,42%} (choice) dan {88,73%} (nonchoice). Hal ini diduga karena Biji kopi Robusta memiliki tekstur biji yang lebih lunak dan lebih tipis daripada biji kopi Arabika dan Excelsa. Biji kopi Robusta juga memiliki kandungan senyawa kimia berupa alkaloid yang salah satu contohnya adalah kafein. Kandungan Kafein pada biji kopi Robusta lebih  tinggi dibandingkan dengan biji kopi Arabika dan biji kopi Excelsa setelah dilakukan uji kandungan kafein.
Activity of an A-L-Rhamnosidase Produced by Aspergillus niger During Solid State Fermentation of Coffee Pulp Wastes Muzakhar, Kahar; Winarsa, Rudju
Jurnal Biodjati Vol 4 No 1 (2019): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i1.4411

Abstract

 An α-L-Rhamnosidase released by Aspergillus niger during solid-state fermentation (SSF) using coffee pulp (CP) wastes media has been investigated. The activity of α-L-Rhamnosidase based on reducing sugar production against 2% CP alkali extract substrate in 50 mM acetate buffer pH 5. The maximum activity of α-L-Rham-nosidase was obtained in sixth-day SSF with reducing sugar pro-duction of 13 μg/mL. The enzyme is actively hydrolyzed 0.1% p-ni-trophenyl-α-L-rhamnopyranoside (PNP-Rha) to 95% from initial concentration. Purification using DEAE-Toyopearl 650M increased hydrolysis activity ten times against the substrate, reaching 134 μg/mL of reducing sugar. Optimum enzyme activity at pH 4.5 and 50°C, while stable at pH and temperature in a pH range of 3.5-7 and below 50°C. 
An Extracellular Pectinase from ISH16 Bacteria Isolated Induced by Coffee Pulp Waste Substrate Muzakhar, Kahar; Elida, Farah Salma; Putrasetya, Ramdhan; Siswoyo, Siswoyo; Winarsa, Rudju; Wiyono, Hidayat Teguh
Jurnal Biodjati Vol 7 No 2 (2022): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i2.20279

Abstract

An α-1,4-glycosidic bonds galactoses pectin, mainly composed of a D-galacturonic acid chain, are important biomaterial widely used in industries. Utilizing this material, a bioprocess, including the biocatalysis pectinase, is often needed. Pectinase production was optimized in 7 days SSF at 37°C, and the pectinase activities were daily measured by the method of Somogy-Nelson. The optimum pectinase production was 0.166 U/ml on the fourth day SSF. Purification using open column ion exchange chromatography DEAE cellulose DE-52 resulted in 1030.9 folds of pectinase purity with a yield of 25.9%. The enzyme was at optimal activity at pH six and attended stable in the pH range of 5.5-8, while optimal activity at a temperature of 50°C and was stable in the range of 30-45°C. The pectinase activity increased by 120% with the addition of 10 mM Mg2+, and 95% retained when 10 mM Ca2+ was added. The presence of 10 mM Na+, K+, and Fe2+ resulted in a slight effect of activity at 85%, 83%, and 78%. However, it was strongly inhibited by 10 mM Al3+ and retained 25%. Based on the results above, the microbial utilization of coffee pulp waste by ISH16 bacteria pectinolytic is one opportunity to produce valuable pectinase with low-cost production, so comprehensive examination in large-scale production is needed too. In this paper, all research detail steps were described.
Co-Authors AA Sudharmawan, AA Agus Subekti Ahmad Bukhari Saragih Alya, Fathia Anisa Azhari, Fitri Dewi, Rosita Fitrah Elida, Farah Salma Esti Utarti Farah Salma Elida Gatot Kusiyanto Hidayat Teguh Wiyono Kartika Senjarini Khafiyya, Nida An Khofiya, Zunairoh Nidaan Khofiya, Zunairoh Nidaan Kusumah, Maulana S. Nuryatmaja Gora Pawana Purwatiningsih Purwatiningsih Purwatiningsih Purwatiningsih Purwatiningsih Purwatiningsih Putrasetya, Ramdhan Ramdhan Putrasetya Reza Billa Afifuddin Rosita Fitrah Dewi Rosyanda Fisel Kosaya S Siswanto Sattya Arimurti Sattya Arimurti Siswanto Siswanto Siswoyo Siswoyo Siswoyo Siswoyo Siswoyo Siswoyo Sururin, Fahma Wardah Sutoyo S Sutoyo Sutoyo Ummi Wasilah Winarsa, Rudju