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All Journal International Journal of Biosciences and Biotechnology Jurnal Ilmu Dasar IPTEK Journal of Proceedings Series BERKALA SAINSTEK Biogenesis: Jurnal Ilmiah Biologi Biotropika Pelita Perkebunan Journal of Mathematical and Fundamental Sciences Jurnal Biodjati BIOSFER : Jurnal Biologi dan Pendidikan Biologi JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Journal of Natural Sciences
Kahar Muzakhar
Department Of Biology, Faculty Of Mathematics And Natural Science, University Of Jember, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Mathematics Physics Public Health Other

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Preliminary Investigation: Stearidonic Acid Production by Genetically Modified Saccharomyces cerreviseae Using Linseed Oil as A Fatty Acid Source Kahar Muzakhar
Jurnal ILMU DASAR Vol 9 No 1 (2008)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (352.305 KB)

Abstract

Stearidonic acid (SDA); 18:4(n-3), an ω-3 Polyunsaturated Fatty Acid (PUFA), could be produced by Genetically Modified Saccharomyces cerreviseae (GMY) using Linseed Oil (LO) as fatty acid source. In nature, S. cerrevisae accumulates only very small amount lipid and contains until mono unsaturated fatty acids. But this GMY strain beside has capability to accumulate lipid, it also contains Δ6 desaturase gene which leads to convert α-Linolenic Acid (ALA); 18:3(n-3) to be SDA. Gas Chromatograph analysis of transmethylated LO sample showed that main component of fatty acid was ALA (about 57%), therefore LO can be used as a cheap source for other PUFA production. In order to provide ALA as a source for the yeast, enzymatic hydrolysis of LO using lipase was done. The lipase from Rhizomucor miehei (L4277-SIGMA) showed the highest activity among 5 lipases when 1 g/L LO in the medium for yeast (containing 6.7g/L yeast nitrogen base, 20 g/L glucose and 2.5g/L tergitol NP-40) was hydrolyzed. For SDA production, 1g/L LO in medium was aseptically hydrolyzed by lipase in 50 unit/mL for 18 hours at 30 oC and 140 rpm. The pre-culture of GMY (1% V/V) was then inoculated into the treated medium and 2.59 g/L dried cells were obtained after 5 days cultivation. ALA was accumulated in the cells at 0.06g/L (11% of total ALA in LO), and only 25% (0.015g/L) of accumulated ALA were converted to SDA. These results suggested that LO can be used as a source for PUFA production. In order to improve the productivity of SDA using GMY, hydrolysis of LO as well as cultivation condition and genetically improvement of the yeast must be highly considerated.
Phosphate Solubilizing Bacteria Adaptive to Vinasse Kahar Muzakhar; Sutoyo Sutoyo; Ahmad Bukhari Saragih
Journal of Mathematical and Fundamental Sciences Vol. 47 No. 2 (2015)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2015.47.2.8

Abstract

Microorganisms identified as phosphate solubilizing bacteria (PSB) adaptive to vinasse were successfully screened from sugarcane soil from an agriculatural estate in Jatiroto. By conducting a screening on Pikovskaya's agar medium (PAM), we found that five different isolates were detected as PSB (pvk-5a, pvk-5b, pvk-6b, pvk-7a, and pvk-8a). Of the five isolates only three could be grown and were found to be adaptive to vinasse based medium without any nutrients added (pvk-5a, pvk-5b and pvk-7a). The three isolates were  characterized as coccus and Gram negative with no endospores detected. We suggest that these three isolates can be used as biofertilizer agent to  support organic farming.
An Extracellular Pectinase from ISH16 Bacteria Isolated Induced by Coffee Pulp Waste Substrate Kahar Muzakhar; Farah Salma Elida; Ramdhan Putrasetya; Siswoyo Siswoyo; Rudju Winarsa; Hidayat Teguh Wiyono
Jurnal Biodjati Vol 7, No 2 (2022): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i2.20279

Abstract

An α-1,4-glycosidic bonds galactoses pectin, mainly composed of a D-galacturonic acid chain, are important biomaterial widely used in industries. Utilizing this material, a bioprocess, including the biocatalysis pectinase, is often needed. Pectinase production was optimized in 7 days SSF at 37°C, and the pectinase activities were daily measured by the method of Somogy-Nelson. The optimum pectinase production was 0.166 U/ml on the fourth day SSF. Purification using open column ion exchange chromatography DEAE cellulose DE-52 resulted in 1030.9 folds of pectinase purity with a yield of 25.9%. The enzyme was at optimal activity at pH six and attended stable in the pH range of 5.5-8, while optimal activity at a temperature of 50°C and was stable in the range of 30-45°C. The pectinase activity increased by 120% with the addition of 10 mM Mg2+, and 95% retained when 10 mM Ca2+ was added. The presence of 10 mM Na+, K+, and Fe2+ resulted in a slight effect of activity at 85%, 83%, and 78%. However, it was strongly inhibited by 10 mM Al3+ and retained 25%. Based on the results above, the microbial utilization of coffee pulp waste by ISH16 bacteria pectinolytic is one opportunity to produce valuable pectinase with low-cost production, so comprehensive examination in large-scale production is needed too. In this paper, all research detail steps were described.
Characterization and Identification of Caffeine-Degrading Bacteria KAJ 36 Sattya Arimurti; Reza Billa Afifuddin; Siswanto Siswanto; Kahar Muzakhar
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 36 No 2 (2020)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v36i2.439

Abstract

Caffeine could be utilized by caffeine-degrading bacteria as a source of carbon and nitrogen. These bacteria have the potential as an agent of decaffeinating coffee. The objective of this research was to characterize and identify the caffeinedegrading bacteria KAJ 36 that was isolated from the pulp waste of Coffea arabica. The isolate-characterization was performed based on its growth and caffeine degradation activity.in a medium M9 added with 10 g/L caffeine for 7 days. The isolate identification was conducted using 16S rRNA sequence analysis and biochemical analysis. The result indicated that caffein-degrading bacteria were able to grow and degrade the caffein up to 86% activity on M9 medium added 10 g/L caffeine for 7 days incubation. Based on 16S rRNA, the isolated KAJ 36 had sequences of 99% homology with Pseudomonas monteilii. Biochemical analysis showed that this isolation on the specific medium was a gram-negative, oxidative, positive enzymatic activity (oxidase, catalase, urease) and reduce nitrate. Our results showed that the isolated KAJ 36 was capable of producing fluorescent pigment. This bacterium was unable to produce indole or utilize sucrose and lactose. Based on the cultural characteristics, we found that the isolated KAJ 36 could be grown in a medium with pH 5, 7, and 9, and in the environment with up to 37oC. This evidence suggested that P. monteilii KAJ 36 can be used as a potential degradation agent of caffeine and recommend for future research in the bacterial based on the degradation of caffeine-contained coffee.
The relationship between environmental factors and the diversity and abundance of flower-visitor insects on chili pepper in dry season Sururin, Fahma Wardah; Muzakhar, Kahar; Khafiyya, Nida An; Purwatiningsih, Purwatiningsih
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 1 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i1.42864

Abstract

Approximately 80% of flowering plants are highly dependent on insect pollination, including the chili pepper (Capsicum frutescens). Environmental conditions serve as parameters for insects in their activities. Therefore, this study was conducted to determine the diversity and abundance of flower-visiting insects in C. frutescens and their environmental factors. Field observations of flower-visiting insects were conducted during the dry season utilizing aerial capture methods. The data was analyzed using diversity, abundance, evenness, and dominance indices equations. Multiple linear regression analysis was also conducted to determine the abiotic and biotic factors (environmental factors) that influence the abundance of flower-visiting insects. The analysis results showed that a moderate trend in the diversity of insect and as many as 33% of the total recorded insect individuals belong to the Vespidae family. The Allorhynchium argentatum showed the highest species abundance (27% individuals). Based on the multiple regression analysis results, environmental factors had a non-significant native influence on the abundance of flower-visiting insects. Environmental conditions during the dry season have a weak influence on insect interactions with plants.
SKRINING BAKTERI SELULOLITIK ASAL VERMICOMPOSTING TANDAN KOSONG KELAPA SAWIT Azizah, Siti Nur; Muzakhar, Kahar; Arimurti, Sattya
BERKALA SAINSTEK Vol 2 No 1 (2014)
Publisher : Universitas Jember

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Abstract

Biomassa Tandan Kosong Kelapa Sawit (TKKS) dihasilkan dalam jumlah melimpah selama pemanenan, sehingga harus didekomposisi dalam waktu singkat. Melalui vermicomposting, TKKS dikonversi menjadi kompos yang berlangsung selama 2-3 bulan, sehingga untuk mempercepat proses dekomposisi, penelitian ini perlu dilakukan. Lima puluh satu isolat bakteri selulolitik berhasil diisolasi dari vermicomposting limbah TKKS. Hasil uji pada media CMC (Carboxymethyl Cellulose) plate, empat isolat memiliki aktivitas selulolitik tertinggi, yaitu isolat 20, 40a, 40b dan 49 dengan indeks aktivitas sebesar 11,90; 10,97; 11,29, dan 11,24. Selama hidrolisis menggunakan substrat CMC dan TKKS, isolat 20 mampu memproduksi gula reduksi tertinggi yaitu sebesar 12,27 μg/mL dan 49,31 μg/mL, sedangkan isolat 40a, 40b, dan 49 sebesar 3,48 μg/mL, 6,28 μg/mL dan 3,10 μg/mL di substrat CMC dan sebesar 24,83 μg/mL, 11,21 μg/mL dan 8,25 μg/mL di substrat TKKS. Keempat isolat bakteri termasuk bakteri Gram negatif dengan bentuk sel batang. Kata Kunci: bakteri selulolitik, gula reduksi, vermicomposting TKKS.
Growth of Lactobacillus casei FNCC0900 in Media Based Umbi Porang Plant (Amorphophallus muelleri BI.) Azhari, Fitri; Winarsa, Rudju; Siswanto, Siswanto; Muzakhar, Kahar; Utarti, Esti; Sutoyo, Sutoyo; Arimurti, Sattya
BERKALA SAINSTEK Vol 9 No 2 (2021)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v9i2.19034

Abstract

Porang tuber (Amorphophallus muellerii BI.) Is a type of tuber that has a high enough glucomannan content of 67%. Glucomannan is very difficult to digest by humans directly so it takes the role of probiotics. L. casei bacteria FNCC0900 as a probiotic agent capable of utilizing glucomannan as a carbon source for growth. The purpose of this study was to determine the growth pattern and changes in environmental factors, namely the pH value of the probiotic bacteria L. casei FNCC0900 growth medium. The parameters in this study consisted of the highest cell density, generation time and pH value changes in Glucose Yeast Peptone Liquid Media, Porang Boiled Water Media and Porang Flour Liquid Media using the drop plate method which had 4 repeated calculations. Porang Boiled Water Liquid Media has a faster log phase period with a higher cell density than Porang Flour Liquid Media, but the shortest generation time is found in Porang Flour Liquid Media with the highest number of generations. L. casei FNCC0900 bacteria are more able to reduce the pH of Glucose Yeast Peptone Liquid Media compared to porang tuber-based media, so in this case L. casei FNCC0900 can be stated to be able to grow on porang tuber-based media with growth patterns, generation time, cell density and pH value. which varies.
Hidrolis Kulit Buah Kopi Oleh Kapang Pestalotiopsis sp. VM 9 Serta Pemanfaatan Hidrolisatnya Sebagai Medium Produksi Protein Sel Tunggal Saccharomyces cerevisiae Khofiya, Zunairoh Nidaan; Winarsa, Rudju; Muzakhar, Kahar
BERKALA SAINSTEK Vol 7 No 1 (2019)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v7i1.9682

Abstract

Hidrolisis limbah kulit buah kopi oleh enzim ekstraseluler Pestalotiopsis sp. VM 9 telah dilakukan. Hasil hidrolisis menunjukkan bahwa dalam hidrolisat mengandung gula reduksi 392,35μg/ml setelah masa inkubasi hari ke-6. Analisis lanjutan menunjukkan bahwa hidrolisat dapat digunakan sebagai medium pertumbuhan Protein Sel Tunggal (PST) S. cerevisiae dengan tingkat pertumbuhan hingga mencapai kepadatan sel 8,5x10 6 sel/ml selama 72 jam kultivasi. Selama pertumbuhannya S. cerevisiae terbukti mengkonsumsi sumber karbon dari gula reduksi sebanyak 211,91 μg/ml.
Efektivitas Pestisida Berbahan Nimba Terhadap Mortalitas Sitophilus zeamais (Coleoptera: Curculionidae) di Penyimpanan Biji-bijian Purwatiningsih, Purwatiningsih; Alya, Fathia Anisa; Winarsa, Rudju; Muzakhar, Kahar
Journal of Natural Sciences Vol 5, No 3 (2024): Journal of Natural Sciences November
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v5i3.677

Abstract

Sitophilus zeamais is known as a warehouse pest insect that can cause damage to post-harvest crops by 0.5-2%. Effective control by considering food safety is the use of neem-based pesticides. This study aims to determine the effect of neem-based pesticides on S. zeamais mortality. The study was designed with a Completely Randomized Design (CRD) consisting of 6 treatments and 3 replications, with a spraying method. Measurement of feed weight before and after pesticide application was also observed. The results showed that neem-based pesticides had a significant effect on insects (p 0.05). Insect mortality at concentration of 0.5% showed the highest mortality with an average of 24%. Neem-based pesticide application also affected the amount of feed weight consumed by S. zeamais. At concentrations of 0.125%, 0.25% and 0.5%, the weight of feed consumed showed a decrease compared to concentrations of 1%, 2% and control. It can be concluded that pesticides containing active ingredients of neem at a concentration of 0.5% have the potential effect to control the activity of S. zeamais in post-harvest products based on the insect mortality and damage activity, especially on rice seeds.
Purification and Characterization of Cellulase of Mold Isolated from Vermicomposting Process of Palm Oil Empty Fruit Bunches Dewi, Rosita Fitrah; Muzakhar, Kahar
Jurnal Biodjati Vol 3 No 1 (2018): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v3i1.2292

Abstract

In previous investigation of vermicomposting process of palm oil empty fruit bunches, five cellulase-producing microorganisms had been isolated. An isolated VTM1 identified as Aspergillus sp. as a cellulase producing mold, and proven produced extracellular cellulase in solid state fermentation of palm oil empty fruit bunches. The obtaining crude cellulase was purified to homogeneity by ammonium sulfate precipitation, gel filtration on Sephadex G-100 chromatography, and finally purified on ion exchange chromatography on DEAE Cellulofine A-500. The yield and purification fold were 12.89% and 107.50, respectively. The purified cellulase was easily released glucose when 5% CMC applied as substrate, as shown by TLC analysis. The purified cellulase had an optimal pH and temperature at 4.0 and 45°C, and was stable at pH 3-7 and 30-50°C, respectively.
Co-Authors AA Sudharmawan, AA Agus Subekti Ahmad Bukhari Saragih Alya, Fathia Anisa Azhari, Fitri Dewi, Rosita Fitrah Esti Utarti Farah Salma Elida Gatot Kusiyanto Hidayat Teguh Wiyono Kartika Senjarini Khafiyya, Nida An Khofiya, Zunairoh Nidaan Khofiya, Zunairoh Nidaan Kusumah, Maulana S. Nuryatmaja Gora Pawana Purwatiningsih Purwatiningsih Purwatiningsih Purwatiningsih Purwatiningsih Purwatiningsih Ramdhan Putrasetya Reza Billa Afifuddin Rosita Fitrah Dewi Rosyanda Fisel Kosaya S Siswanto Sattya Arimurti Sattya Arimurti Siswanto Siswanto Siswoyo Siswoyo Siswoyo Siswoyo Sururin, Fahma Wardah Sutoyo S Sutoyo Sutoyo Ummi Wasilah Winarsa, Rudju