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Penggunaan Serbuk Infus Bekatul Sebagai Bahan Baku Bekatul Dextrosa Agar Untuk Pertumbuhan Jamur Mujahidah - Basarang; Mardiah Mardiah; Andi Fatmawati
Jurnal Ilmu Alam dan Lingkungan Vol. 11 No. 1 (2020): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (475.64 KB) | DOI: 10.20956/jal.v11i1.9310

Abstract

Rice bran is a waste of rice processing that can be used as a growth media of fungi because it contains high carbohydrates and high protein. Rice bran infusion is made in powder form using freeze-drying techniques to maintain the nutritional content of rice bran. This study aims to determine the level of carbohydrate and protein media made from bran infusion powder and determine the dose of bran infusion powder in the preparation of dextrose bran media. In this study using the Luff Schoorl method to measure the levels of carbohydrate media and the Kjeldhal method to measure the protein content of bran media. Candida albicans, Mallasezia furfur, Aspergillus fumigatus were inoculated in each medium. From this study, the highest levels of carbohydrate (9.11%) and protein (1.64%) were obtained in media using 200 g of rice bran infusion (iBDA200) and the lowest levels carbohydrate (0.44%) and protein (0.08%) wer obtained in media using 2 g of rice bran infusion powder (SBDA2). Growth of M. furfur, C. albicans and A. fumigatus the most fertile on iBDA200 media. This study shows that carbohydrate and protein content are higher in media made from bran infusion. 10 g of rice bran infusion powder (SDA10) best grows fungi but has no significant correlation with fungi growth.
Deteksi Kelompok Enterobacteriaceae pada Tanah di Lingkungan Tempat Pembuangan Akhir Sampah Tamangapa Kecamatan Manggala Makassar Tuty Widyanti; Andi Fatmawati
Jurnal Ilmu Alam dan Lingkungan Vol. 13 No. 1 (2022): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jal.v13i1.20453

Abstract

Garbage is one of the problems faced, especially in big cities throughout developed and developing countries which can lead to environmental pollution such as soil pollution. Soil that contains many sources of pollutants from waste allows bacteria to live in that place, both pathogenic and non-pathogenic bacteria such as Coliform bacteria, where their presence is an indicator of biological environmental pollution. This does not rule out the possibility of finding other pathogenic bacteria such as bacteria from the Enterobacteriaceae group such as Salmonella, Shigella, Escherichia coli, Klebsiella, Enterobacter, Serratia, Proteus, etc. which cause infectious diseases in the digestive tract. This study used soil samples taken in 5 environmental areas of the Tamangapa Final Disposal Site in the District, Manggala Makassar randomly. The purpose of the study was to detect groups of Enterobactericeae in the soil in the Tamangapa Final Disposal Site in the district, Manggala Makassar. This study uses an identification method based on biochemical tests automatically using the VITEK 2TM Compact, bioMérieux, SA tool. Based on the research that has been carried out, the results obtained are that there are types of bacteria of the Enterobacteriaceae group detected in the environmental soil of the Tamangapa Final Disposal Site in the District, Manggala Makassar, namely Enterobacter cloacae, Klebsiella pneumonia, and Escherichia coli which are classified as species that cause pathogenic diseases of the digestive tract
Analisis Mikroflora Candida albicans pada Perokok dan Potensi Daya Hambat Ekstrak Daun Pacar Kuku Lawsonia sp. Terhadap Isolat Candida albicans Andi Fatmawati; Tuty Widyanti; Anita
Jurnal Ilmu Alam dan Lingkungan Vol. 13 No. 1 (2022): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jal.v13i1.20456

Abstract

Salah satu mikroflora normal pada mulut yang dipengaruhi oleh keberadaan asap rokok adalah Candida albicans, jamur ini dapat berubah menjadi patogen jika terjadi perubahaan dalam diri host. Perubahan yang terjadi pada host tersebut dapat bersifat lokal maupun sistemik yang dapat menyebabkan kandidiasis oral. Daun pacar kuku (Lawsonia inermis) merupakan tanaman obat yang mengandung senyawa aktif yang berkhasiat yaitu senyawa lawsone (2-hydroxy-1, 4-napthoquinone). Selain itu daun pacar kuku juga mengandung alkaloid, glikosida, flavonoid, fenol, saponin, tannin, dan minyak atsiri. Salah satu khasiatnya adalah sebagai antijamur. Penelitian ini bertujuan mengetahui kolonisasi Candida albicans dari swab mukosa perokok dan kemampuan penghambatan ekstrak daun pacar kuku terhadap isolat Candida albicans. Penelitian ini bersifat ekperimental laboratorium dengan menggunakan teknik isolasi jamur secara swab dan metode uji daya ekstrak daun pacar dengan konsentrasi 15%, 25%, 50%, 75% dan 100%. Hasilnya menunjukkan bahwa 90% swab mukosa mengandung kolonisasi Candida albicans dan uji ekstrak daun pacar kuku memperlihatkan terbentuknya zona bening tertinggi pada konsentrasi 100% dengan diameter sebesar 15.4 mm. Hal ini menunjukkan bahwa ekstrak daun pacar kuku memiliki potensi inhibitor terhadap isolat Candida albicans dari swab mukosa perokok.
IDENTIFIKASI RHODAMIN B PADA JAJANAN AGAR-AGAR YANG DIPERJUALBELIKAN DI PASAR SUNGGUMINASA KABUPATEN GOWA Darmawaty Rauf; Rahmawati Rahmawati; Andi Fatmawati; Ayu Amelia Sain
Jurnal Medika Vol 7 No 1 (2022): Juni 2022
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Muhammadiyah Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53861/jmed.v7i1.289

Abstract

Jajanan agar-agar merupakan makanan berbentuk gel yang diolah dari rumput laut dan memiliki berbagai aneka rasa dan warna sehingga banyak produsen menyalahgunakan zat pewarna berbahaya sebagai pewarna makanan salah satunya adalah Rhodamin B yang biasanya digunakan untuk produksi makanan, kosmetik dan obat-obatan. Menurut Peraturan Menteri Kesehatan RI No.239/MenKes/Per/V/85 dinyatakan sebagai zat pewarna berbahaya dan dilarang digunakan pada produk pangan karena dapat menyebabkan iritasi pada saluran pencernaan dan jika mengkonsumsi secara terus menerus akan menyebabkan kerusakan organ tubuh dan mengakibatkan kematian. Penelitian ini bertujuan untuk mengidentifikasi Rhodamin B pada jajanan agar-agar yang diperjualbelikan di pasar Sungguminasa Kabupaten Gowa. Jenis penelitian observasi laboratorik dengan teknik pengambilan sampel secara purposive sampling yang menggunakan 10 sampel jajanan agar-agar yang diperjualbelikan di pasar Sungguminasa Kabupaten Gowa. Metode penelitian yang digunakan yaitu kromatografi lapis tipis (KLT), dari 10 sampel yang diuji menunjukkan hasil negatif atau tidak adanya bercak seperti pada kontrol positif. Dengan demikian dapat disimpulkan bahwa tidak terdapat Rhodamin B pada jajananan agar-agar yang diperjualbelikan di pasar Sungguminasa Kabupaten Gowa.
UJI EFEKTIVITAS PERASAN TEH (Camellia sinensis L.) CELUP TERHADAP Staphylococcusaureus Waode Rustiah; Andi Fatmawati; Dewi Arisanti; Muawanah Muawanah; Nuraedah Hasima
Jurnal Medika: Media Ilmiah Analis Kesehatan Vol 7 No 2 (2022): Desember 2022
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Muhammadiyah Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53861/jmed.v7i2.293

Abstract

Camellia sinensis L. atau yang lebih dikenal daun teh hijau sudah lama digunakan sebagai obat herbal dan juga bisa dijadikan olahan produk makanan maupun minuman. Kandungan polifenol, katekin, dan tanin yang memiliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengetahui perasan teh celup (Camellia sinensis L.) dalam menghambat pertumbuhan bakteri Staphylococcus aureus. Penelitian ini dilaksanakan secara eksperimen laboratorik dengan variasi konsentrasi, yaitu 25%, 50%, dan 75% serta larutan pembanding kontrol positif (antibiotic tetracycline) dan larutan pembanding kontrol negatif (aquabides), dengan masa inkubasi selama 24 jam pada suhu 370C. Sampel dalam penilitian ini adalah perasan teh hijau celup (Camellia sinensis L.) menggunakan metode difusi agar berlapis. Berdasarkan hasil penelitian pada konsentrasi 25%, 50% dan 75% tidak memiliki zona hambat. Pada kontrol negatif memiliki zona hambat 0 mm sedangkan kontrol positif memiliki zona hambat 20,2 mm. Hasil penelitian ini menunjukkan bahwa perasan teh celup tidak efektif menghambat pertumbuhan bakteri Staphylococcus aureus
Identifikasi Dan Hitung Jumlah Bakteri Pada Telapak Tangan Penjual Gorengan Anita Anita; Muawanah Muawanah; Andi Fatmawati; Rahmawati Rahmawati; Faisal Faisal
Lontara Journal of Health Science and Technology Vol 4 No 1 (2023): Ilmu dan Teknologi Kesehatan
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Muhammadiyah Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53861/lontarariset.v4i1.368

Abstract

The palm is the part of the body that is most often used daily to carry out various activities, which makes hands very easily contaminated by bacteria. This causes the palm of the hand can act as a medium for transmission of various types of diseases. This study aims to identify and count the number of bacteria on the palms of fried food sellers. This research technique was carried out by laboratory observation with the object of research being the palms of 10 fried food sellers in Rappocini District, Makassar City. Based on the results of research that has been carried out from 10 samples, it has been identified microscopically the form of gram-negative bacilli is red and clustered and macroscopic identification shows the shape of bacterial colonies is round, jagged with small to large sizes, while the results of calculating the number of bacteria for all samples obtained the number of bacteria in large numbers (TBUD). It can be concluded that the palms of fried food sellers in Rappocini District, Makassar City are positive for gram-negative bacilli with an amount that cannot be counted (TBUD).
GAMBARAN KADAR GULA DARAH SEWAKTU DAN TINGKAT PENGETAHUAN MASYARAKAT DUSUN PIMPINGA DESA BATURAPPE KECAMATAN BIRINGBULU KABUPATEN GOWA Rahmawati Rahmawati; Andi Fatmawati; Nurhidayat Nurhidayat; Atri Rahmi
Lontara Abdimas : Jurnal Pengabdian Kepada Masyarakat Vol 4 No 1 (2023): Juni
Publisher : Lembaga Penelitian Dan Pengabdian Masyarakat Politeknik Kesehatan Muhammadiyah Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53861/lomas.v4i1.354

Abstract

Blood sugar is a medical term that can determine the level of glucose in the blood. Health tests such as blood sugar checks are markers or tools to identify metabolism in the body. Glucose levels usually rise after eating and usually fall before eating. This service activity aims to find out the description of current blood sugar levels (GDS) and the level of knowledge of the people of Pimpinga Hamlet, Baturappe Village, Biringbulu District, Gowa Regency. Examination of GDS with the POCT method using a kit (Gluco-test) or glucometer. The number of participants was around 56 people and there were more women than men. Before carrying out the GDS examination, interviews were conducted with participants to explore medical history or hereditary diseases. The examination results showed that there were 42 participants (75%) who had normal blood sugar levels, and 14 participants (25%) who had blood sugar levels above normal values (> 200 mg/dl). Thus it can be concluded that the normal blood sugar levels (GDS) of the community members are generally normal because they have paid attention to the pattern of healthy food intake by consuming vegetables.
PEMERIKSAAN KADAR BAHAN KIMIA OBAT (BKO) NATRIUM DIKLOFENAK PADA JAMU PEGAL LINU Waode Rustiah; Hasnah Hasnah; Andi Fatmawati; Muawanah Muawanah; Yustikasari Masulili
Jurnal Medika Vol 8 No 1 (2023): Juni 2023
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Muhammadiyah Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53861/jmed.v8i1.370

Abstract

Natrium diklofenak adalah derivat asam fenilasetat yang secara spesifik dikembangkan sebagai agen antiinflamasi. Natrium diklofenak merupakan anggota grup arilalkanoat, obat golongan non-steroidal anti-inflamatory drugs (NSAID) yang digunakan oleh masyarakat sebagai salah satu terapi artritis rematoid, osteoartritis, ankilosa, spondilitis dan inflamasi oftalmik. Natrium diklofenak biasanya banyak ditemukan dalam produk jamu kemasan. Jamu merupakan obat tradisional yang berperan penting dalam pengobatan penduduk di negara berkembang. Dalam jamu, obat ini bekerja dengan cara menghentikan produksi zat penyebab rasa sakit. Penelitian ini bertujuan untuk mengetahui ada atau tidaknya bahan kimia obat Natrium diklofenak dalam jamu pegal linu yang diperjualbelikan di pasar tradisional kota Makassar. Teknik pengambilan sampel menggunakan cara random sampling. Sebanyak 10 sampel dilakukan secara kualitatif dengan metode kromatografi lapis tipis (KLT) dan kuantitatif menggunakan spektrofotometeri UV-Vis. Hasil penelitian menunjukkan bahwa dari 10 sampel yang diteliti semuanya teridentifikasi positif natrium diklofenak yang ditunjukkan dengan nilai Rf sampel jamu dengan standar natrium diklofenak yang sama yaitu 0,85, karena selisih Rf sampel terhadap standar ≤ 0,2. Selanjutnya, dilakukan uji kuantitatif dengan spektrofotometer UV-Vis, diperolah kadar rata-rata 13,956 ppm. Dari hasil penelitian ini disimpulkan bahwa produk jamu tersebut tidak layak untuk mendapatkan izin edar.
Uji Aktivitas Ekstrak Perasan Daun Kedondong (Spondias dulcis) Sebagai Anti-Candida albicans pada Penderita Diabetes Melitus Anita Anita; Andi Fatmawati; Tuty Widyanti; Rahmawati Rahmawati; Putri Ahmad
Lontara Journal of Health Science and Technology Vol 4 No 2 (2023): Ilmu dan Teknologi Kesehatan
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Muhammadiyah Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53861/lontarariset.v4i2.418

Abstract

Diabetes is a disease characterized by high blood glucose levels which can stimulate the growth of the Candida albicans. Indonesia is the second highest country in the world with biodiversity which allows the creation of new compounds that can be used as medicine. One type of plant that has medicinal properties is kedondong leaves (Spondias dulcis) which can be used as herbal medicine, and can also act as an anti-fungal, because it has anti-fungal substances such as flavonoids, alkaloids, saponins and tannins. This compound can prevent fungal growth. The aim of this research was to determine the inhibitory ability of kedondong (Spondias dulcis) leaf extract in inhibiting the growth of Candida albicans using the Kirby Bauer method. The type of research used in this research is laboratory experiments. The results of the inhibition test in this study were the concentration inhibition zone results of 25%, 50%, 75%, 100%, the results were 0 mm, indicating that these 4 concentrations could not inhibit the growth of Candida albicans. Meanwhile, the positive control showed that there was a clear zone with an average of 28.8 mm, which indicated that antibiotics inhibited the growth of Candida albicans, while the negative control showed that no clear zone was formed. Therefore, the results of this study can be concluded that squeezed kedondong (Spondias dulcis) leaf extract cannot inhibit the growth of Candida albicans isolated from diabetes mellitus sufferers
Studi Literatur Identifikasi Jamur Pada Kuku dan Sela Kaki Penderita Diabetes Melitus Anita Anita; Nurul Ni’ma Aziz; Andi Fatmawati; Novalin Moiyo
Lontara Journal of Health Science and Technology Vol 1 No 2 (2020): Ilmu dan Teknologi Kesehatan
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Muhammadiyah Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53861/lontarariset.v1i2.75

Abstract

Diabetes mellitus is a disease characterized by hyperglycemia (increased blood sugar levels) that occurs due to abnormalities in insulin secretion, insulin action or both. High blood glucose levels can cause an increase in glucose levels in the skin of diabetes mellitus patients, thereby facilitating the appearance of skin manifestations in the form of dermatitis, bacterial infections, fungal infections and others. The condition of epithelial and mucosal cells of diabetes mellitus sufferers has increased adhesion to several groups of fungi on epithelial cells, namely mold and yeast. This type of research is a literature study that summarizes as many as 10 journals that are relevant to these studies. From the literature study, it turned out that 225 samples were obtained with details of 65 samples (28.9%) tested positive for Trichophyton rubrum, Trichophyton mentagrophytes, Epidermophyton floccosum and 160 samples (71.1%) tested negative. Based on the data from the literature study, it can be concluded that people with diabetes mellitus are at risk of being infected with the fungi that cause tinea pedis and tinea ungium, because the condition of the epithelial and mucosal cells has increased so that it makes it easier to develop fungal infections.