Anita Dwi Anggraini
Department Of Medical Laboratory Technology, Poltekkes Kemenkes Surabaya, Surabaya, Indonesia

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POTENSI METABOLIT SEKUNDER ANTIFUNGI AKTINOMISETES YANG DIISOLASI DARI TANAH MANGROVE WONOREJO SURABAYA TERHADAP Trichophyton rubrum Febrianti Fitria; Sasongkowati Retno; Anggraini Anita Dwi
Analis Kesehatan Sains Vol. 10 No. 1 (2021): Analis Kesehatan Sains
Publisher : Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Surabaya

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Abstract

Actinomycetes are gram-positive bacteria that have aerobic properties. Actinomycetes have the ability to produce antibiotic compounds. Actinomycetes are the second largest organisms that live in soil with a population of 1-10 million per gram of soil. Actinomycetes also make up 10-50% of the microbes in the soil. The purpose of this study was to determine the antifungal activity of actinomycetes isolates isolated from the soil of the Mangrove Wonorejo forest in Surabaya against the fungus Trichophyton rubrum. This type of research is an experimental laboratory with quantitative analysis. Actinomycetes isolates were taken from 3 different soil locations, pretreatment by heat treatment at 90°C for 15 minutes. Actinomycetes were isolated on casein starch agar medium which had been added with 0.002% Nystatin. Selection of antimicrobial compounds was based on the disc diffusion method which was characterized by the formation of a zone of inhibition around the disc. The results showed that from 3 locations of soil sampling, 8 actinomycetes isolates were obtained, but only 6 isolates were able to inhibit the fungus Trichophyton rubrum. showed that isolates C3-2, B3-1, C4-1, were categorized as having very strong inhibitory power. While isolates A5-1, A3-2 were categorized as having inhibitory power and isolates A4-1 and A4-2 were categorized as having moderate inhibitory power against the fungus Trichophyton rubrum.
HUBUNGAN KEJADIAN INFEKSI Soil transmitted helminths (STH) dengan NILAI EOSINOFIL dan HEMOGLOBIN DARAH pada MASYARAKAT di KAMPUNG 1001 MALAM SURABAYA Okvitasari Arin; Sasongkowati Retno; Anggraini Anita Dwi
Analis Kesehatan Sains Vol. 10 No. 2 (2021): Analis Kesehatan Sains
Publisher : Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Surabaya

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Abstract

Soil worm infection transmitted Helminths (STH) is a group of parasitic nematodes that cause infection in the human gut which is transmitted through contaminated soil eggs or larvae. STH often infects people with poor environmental sanitation and lack of public awareness of the importance of maintaining personal and environmental hygiene. An increase in the value of eosinophils and a decrease in the value of blood hemoglobin are markers of infection by the STH parasite. The purpose of this study was to determine the relationship between the incidence of soil-transmitted helminths (STH) infection with the value of eosinophils and blood hemoglobin in the 1001 night village community, Surabaya. This type of research is an analytical observational study and the samples in this study were blood and feces taken from the people of 1001 Malam Surabaya village as many as 25 people with a random sampling technique using the native method for stool examination and using a hematology analyzer for blood examination. Based on the results of research conducted on the 1001 night village community, it was known that the prevalence of worm infection was Ascaris lumbricoides. The prevalence of STH infection with eosinophilia was 15 samples. A significant relationship after being tested statistically with the Spearman Rank Correlation test was found between STH infection and the eosinophil value of 0.034. Then on the relationship between hemoglobin values and STH infection, a significant value was obtained, namely 0.00. There is a significant relationship between STH infection with the value of eosinophils and blood hemoglobin.
Expression of Immunoglobulin G / Immunoglobulin M Typhoid On Neutrophil-Lymphocyte Ratio In Patients At Haji Hospital, Surabaya Nur Zerlinda; Evy Diah Woelansari; Anita Dwi Anggraini
International Journal of Advanced Health Science and Technology Vol. 3 No. 4 (2023): August
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ijahst.v3i4.289

Abstract

Typhoid fever is one of the major bacterial infections worldwide caused by the bacterium Salmonella enterica serovars typhi in humans. The examination used is the rapid Typhoid test. This rapid typhoid test is used to detect antibodies to Salmonella typhi. The purpose of the study was to determine the results of the Neutrophil - Lymphocyte Ratio with various criteria results from the Immunoglobulin G / Immunoglobulin M Test in typhoid fever patients. This type of study is descriptive observational with selective sampling taken. The samples in this study were 28 samples conducted in April 2023 on typhoid fever patients at the Hajj Hospital in East Java Province. This study used the Rapid Typhoid Test Immunochromatography method with positive Widal criteria and supporting examination using Neutrophil–Lymphocyte Ratio (NLR) with Hematology Analyzer tool Fluorescent Flow Cytometry method. Typhoid and NLR rapid test results are processed descriptively in tables and based on percentages. The results obtained from the rapid test were positive IgM of 29%, positive IgG of 11%, positive IgG and IgM of 3%, and negative IgG and IgM of 57%. The average NLR was 4,43% with a median of 2,85. IgG / IgM examination of NLR showed no significant association in typhoid fever patients. It is hoped that this research will be further developed using Salmonella culture examination.