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All Journal Window of Health : Jurnal Kesehatan INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) International Journal of Advanced Health Science and Technology Jurnal Pengabdian Kepada Masyarakat Mulawarman Analis Kesehatan Sains (e-Journal)
Edy Haryanto
Department Of Medical Laboratory Technology, Health Polytechnic Ministry Of Health Surabaya, Indonesia
Biochemistry, Genetics & Molecular Biology Chemistry Electrical & Electronics Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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In Silico Analysis of Antiviral Activity and Pharmacokinetic Prediction of Brazilein Sappan Wood (Caesalpinia sappan L.) Against SARS-CoV-2 Spike Glycoproteins Dwi Krihariyani; Edy Haryanto; Retno Sasongkowati
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i1.1854

Abstract

Brazilein is one of the secondary sappan wood metabolites which can be used empirically as an antivirus. The SARS-CoV-2 spike (S) glycoproteins play significant roles in attaching and entering the virus into the host cell. This study aims to predict the antiviral activity and pharmacokinetic properties of brazilein of the sappan wood against the in-silico SARS-CoV-2 S glycoproteins with vitamin C as the reference compound. Molegro Virtual Docker 5.5 was used to predict antiviral activity by docking process. SARS-CoV-2 S glycoprotein with NAG ligand available in Protein Data Bank (PDB) (PDB ID: 7C01) was the receptor used. The pkCSM online tool was used to predict the pharmacokinetic properties and toxicity of brazilein. Data were analyzed on the target receptors by comparing the docking bond energies between NAG, brazilein, and vitamin C. The smaller the ligands’ bond energy to the target receptor, the more stable the bonds are. The bond energy of NAG, brazilein, and vitamin C was -59.2864 kcal/mol, -65.8911 kcal/mol, and -53.9093 kcal/mol, respectively. These results suggested that brazilein has a greater capacity as an antivirus compared to NAG and vitamin C. In silico test using the pkCSM online tool demonstrated that brazilein had strong pharmacokinetic properties and relatively low toxicity.
Diagnostic test of Rapid antigen SARS Cov-2 against RT-PCR on suspected symptomatic COVID-19 patients at Rato Ebu Hospital Bangkalan Anik Handayati; Edy Haryanto
International Journal of Advanced Health Science and Technology Vol. 2 No. 3 (2022): June
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ijahst.v2i3.3

Abstract

COVID-19 is caused by the SARS-CoV-2 virus. Two tests to detect the SARS-CoV-2 virus are the rapid antigen test and RT-PCR. The gold standard for testing for COVID-19 is RT-PCR. The high number of Covid-19 sufferers in Madura plus the RT-PCR examination takes a long time. Rapid antigen examination is one of the Covid-19 screening solutions that should be tested because it offers fast examination times. This study aims to determine the sensitivity and specificity of the SARS COV-2 rapid antigen test to RT PCR for the diagnosis of COVID 19. This type of research is an analytical study with a cross-sectional design. The study was conducted at Rato Ebu Hospital Bangkalan from July-September 2021. The sample of this study was suspected symptomatic COVID-19 patients who were examined using the SARS-CoV-2 rapid antigen and RT-PCR using purposive sampling of as many as 60 people. Diagnostic test method by measuring the sensitivity and specificity of rapid antigen to RT-PCR. Based on the results of the study, it can be concluded that the sensitivity of the SARS Cov-2 Rapid Antigen to RT-PCR is 82.97% and the specificity of the SARS Cov-2 Rapid Antigen to RT-PCR is 100%.
Relationship of Albumin Concentrations with Sodium, Potassium, in CAPD (Continuous Ambulatory Peritoneal Dialysis) Patients at PHC Hospital Surabaya Dian Anggraini; Edy Haryanto; Syamsul Arifin; Ayu Puspitasari
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 4 No 2 (2022): Enhanced knowledge of laboratory medicine's role in healthcare
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v4i2.3252

Abstract

Kidney failure is caused by a permanent decline in kidney function and requires renal replacement therapy, one of which is Continuous Ambulatory Peritoneal Dialysis (CAPD). This study aimed to determine the relationship between albumin concentrations and sodium and potassium concentrations in CAPD patients. The study was carried out between January to May 2022. Cross-sectional research methodology is used in this study. It included 60 chronic kidney failure patients who had CAPD as therapy at PHC Surabaya Hospital. Then, patients were divided into three groups with an age interval of 40-50 years, 51-60 years, and 61-70 years in. The results of grouping CAPD patients according to the characteristics based on the age of CAPD patients aged 61-70 years had low albumin concentrations and low sodium concentrations A total of 12 people (66.67%), patients with large CAPD aged 51-60 years, had low albumin and potassium concentrations, namely 11 people (61.11%). CAPD patients aged 51-60 years mostly had low albumin and sodium concentrations in 13 people (72.22%). the conclusion is that in patients with renal failure on CAPD therapy, there is a decrease in albumin and sodium-potassium concentrations due to peritonitis (inflammation) which is associated with a measurable excess of fluid (overhydration) which causes more dilute sodium, causing hyponatremia and hypokalemia.
STUDI IN SILICO SIFAT FARMAKOKINETIK, TOKSISITAS, DAN AKTIVITAS IMUNOMODULATOR BRAZILEIN KAYU SECANG TERHADAP ENZIM 3-CHYMOTRYPSIN-LIKE CYSTEINE PROTEASE CORONAVIRUS Dwi Krihariyani Dwi; Retno Sasongkowati; Edy Haryanto
Journal of Indonesian Medical Laboratory and Science Vol 1 No 1: Oktober 2020
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v1i1.14

Abstract

The 3-chymotrypsin-like cysteine ??protease (3CLpro) is an enzyme that control replication of the coronavirus life cycle. The 3CLpro is a biological target that will interact with drugs. Brazilein is a major compound found in secang wood that used as an immunomodulator. This study aims to predict the immunomodulatory activity of the brazilein against the 3CLpro using in silico method, with curcumin as comparative compound. In silico test was used to predict immunomodulatory activity by docking using the Molegro Virtual Docke. Receptors used are 3CLpro, PDB code: 6M2N, with ligand 3WL_401[C]. pkCSM online program used to predict pharmacokinetic properties (ADME) and toxicity from brazilein and curcumin. Data was analyzed by comparing the docking bond energy between brazilein, ligands and curcumin at the target receptor. The lower the ligand bond energy, the more stable the bond is formed. The results of the in silico test showed that the ligand bond energy = -83,1153kcal/mol, brazilein = -82.0583kcal/mol, and curcumin = -115.852kcal/mol. Brazilein has lower potential as an immunomodulator than ligands and curcumin. The results of the in silico test using the pkCSM online program showed that the brazilein compound had good pharmacokinetic properties and low toxicity.
Pendampingan dan Peningkatan Pengetahuan Manfaat Infusa Bawang Putih pada Masyarakat dalam Mencegah dan Mengatasi Hipertensi pada Masyarakat di Desa Ketetang Kecamatan Kwanyar Kabupaten Bangkalan Edy Haryanto; Sri Sulami Endah Astuti; Retno Sasongkowati; Anita Dwi Anggraini
ABDIKESMAS MULAWARMAN : Jurnal Pengabdian Kepada Masyarakat Vol.3 No.1 Mei (2023) : ABDIKESMAS MULAWARMAN
Publisher : Universitas Mulawarman

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Abstract

Hipertensi merupakan penyakit degeneratif yang menjadi masalah serius saat ini. Bahaya hipertensi yang tidak dapat dikendalikan dapat menimbulkan komplikasi yang berbahaya, seperti penyakit jantung koroner, stroke, ginjal dan gangguan penglihatan. Beberapa faktor yang berperan dalam terjadinya hipertensi meliputi faktor mayor yaitu faktor risiko yang tidak dapat dikendalikan dan faktor minor yaitu faktor risiko yang masih dapat dikendalikan. Permasalahan yang terjadi pada masyarakat adalah kurangnya pengetahuan tentang pengendalian penyakit hipertensi serta faktor-faktor yang mempengaruhi kejadian hipertensi dan ketidaktahuan masyarakat dalam memanfaatkan bahan herbal yang ada disekitar untuk dimanfaatkan untuk menurunkan hipertensi yaitu contohnya bawang putih. Kegiatan pengabdian masyarakat yang dilakukan pada warga di Desa Ketetang dusun kwalas berhasil dilaksanakan dan diikuti sebanyak 31 orang yang mengikuti pemeriksaan tekanan darah, kegiatan pelatihan dan penyuluhan pembuatan dan pemanfaatan infusa bawang. Masalah kesehatan yang dikeluhkan oleh warga mampu diatasi oleh tim pengabdian masyarakat dengan memberikan edusi pelatihan dan penyuluhan pembuatan infusa bawang putih sebagai terapi dari herbal untuk menurunkan tekanan darah. Selama kegiatan berlangsung peserta sangat antusias, hal ini dapat dilihat pada keaktifan peserta mengikuti dari awal sampai akhir.
Pendampingan dan Pemanfaatan Infusa Jahe untuk Meningkatkan Imunitas Tubuh pada Para Petani yang Terinfeksi Kecacingan di Desa Ketetang Kecamatan Kwanyar Kabupaten Bangkalan Sri Sulami Endah Astuti; Edy Haryanto; Syamsul Arifin; Anita Dwi Anggraini
ABDIKESMAS MULAWARMAN : Jurnal Pengabdian Kepada Masyarakat Vol.3 No.1 Mei (2023) : ABDIKESMAS MULAWARMAN
Publisher : Universitas Mulawarman

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Abstract

Pekerjaan yang sangat erat kaitannya dengan infeksi STH yang berhubungan dengan tanah yaitu petani. Dusun Ketetang merupakan desa terpencil yang mayoritas penduduknya bekerja sebagai petani. Petani yang dalam kesehariannya bekerja tidak menggunakan alat pelindung diri seperti sarung tangan dan alas kaki, yang secara langsung kontak dengan tanah, selain itu para petani juga mengonsumsi makanan tanpa mencuci tangan dengan bersih terlebih dahulu. Petani dapat terinfeksi cacing baik melalui oral yaitu melalui makanan dan minuman yang tercemar dan melalui penetrasi kulit dengan adanya kontak langsung dengan kotoran hewan yang digunakan sebagai pupuk tanaman . Penelitian angka kecacingan tahun 2019 yang dilakukan oleh Lisma mahasiswa D3 Jurusan Analis Kesehatan menunjukkan hasil yaitu terd apat 40 responden (80%) yang infeksi Soil Transmitted Helminths dan 10 responden (20%) yang tidak terinfeksi Soil Transmitted Helminths pada petani di Koalas Bangkalan Madura. Ang ka kecacingan yang tinggi ini juga diikuti oleh penurunan kadar hemoglobin yang merupakan dampak akibat infeksi kecacingan. Hasil ini menunjukkan angka kecacingan yang tinggi disertai anemia sehingga masyarakat di Desa Ketetang masih memerlukan edukasi tentang pentingnya Pola Hidup Bersih dan sehat dan penggunaan Alat Pelindung diri untuk mencegah penyakit kecacingan STH, serta mengetahui dan mengkonsumsi jenis makanan sehat yang mampu meningkatkan kadar hemoglobin. Masalah ini terjadi karena kurangnya para petani dalam menjaga sistem imun tubuh sehingga mudah terinfeksi parasit, serta kurangnya pengetahuan tentang pemanfaatan herbal khususnya jahe merah dalam menjaga kesehatan diri oleh para petani. Solusi yang ditawarkan adalah menggerakkan para petani untuk mengkonsumsu infusa jahe dan mengedukasi pentingnya menjaga sistem imun, pembuatan infusa jahe yang benar sehingga nilai gizi dan manfaatnya tidak hilang.
PERBEDAAN KADAR KOLESTEROL HDL DAN KADAR KOLESTEROL TOTAL PADA PEROKOK AKTIF DENGAN PEROKOK PASIF YANG MENDERITA DIABETES MELITUS DI PUSKESMAS TANJUNGANOM KABUPATEN NGANJUK Sadgassusi Hera Samudra; Edy Haryanto; Syamsul Arifin; Wisnu Istanto
Analis Kesehatan Sains Vol. 10 No. 1 (2021): Analis Kesehatan Sains
Publisher : Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36568/anakes.v10i1.9

Abstract

ABSTRACTCigarettes contain various harmful substances that can endanger health. One of them is nicotine which causeslipid metabolism disorders, including a decrease in HDL cholesterol levels and an increase in total cholesterollevels. Smoking habits in people with comorbidities such as diabetes mellitus can exacerbate existingdyslipidemia conditions. If not managed properly, it can cause complications that lead to increased mortality inpeople with diabetes mellitus. This study aims to determine the differences in HDL cholesterol and totalcholesterol levels in active smokers and passive smokers patients with diabetes mellitus at PuskesmasTanjunganom, Nganjuk Regency. This research uses observational study with a cross sectional approach whichwas conducted in January-May 2021 on 30 patients with diabetes mellitus at Puskesmas Tanjunganom, NganjukRegency, consisting of 15 active smokers and 15 passive smokers. Checking total cholesterol levels was carriedout at Puskesmas Tanjunganom, Nganjuk Regency. While the examination of HDL cholesterol levels wascarried out at UPT Labkesda, Nganjuk Regency. Based on the independent sample t-test statistical test, it wasfound that the p-value of difference HDL cholesterol levels in active smokers and passive smokers with diabetesmellitus was 0,000 and the difference total cholesterol levels in active smokers and passive smokers withdiabetes mellitus was 0.012. So it can be concluded that statistically there are differences in HDL cholesteroland total cholesterol levels in active smokers and passive smokers who suffer from diabetes mellitus atPuskesmas Tanjunganom, Nganjuk Regency.Keywords: Diabetes Mellitus; Cholesterol Levels; Active Smoker; Passive SmokerABSTRAK Rokok mengandung berbagai zat berbahaya yang dapat menggangu kesehatan. Salah satunya nikotin yangmengakibatkan gangguan metabolisme lipid antara lain penurunan kadar kolesterol HDL dan peningkatan kadarkolesterol total. Kebiasaan merokok pada orang dengan penyakit penyerta seperti diabetes melitus dapatmemperburuk kondisi dislipidemia yang sudah ada. Apabila tidak dikelola dengan baik dapat mengakibatkankomplikasi yang menyebabkan peningkatan mortalitas penderita diabetes melitus. Penelitian ini bertujuan untukmengetahui perbedaan kadar kolesterol HDL dan kolesterol total pada perokok aktif dengan perokok pasif yangmenderita diabetes melitus di Puskesmas Tanjunganom Kabupaten Nganjuk. Penelitian ini merupakan penelitanobservasional dengan pendekatan cross sectional yang dilakukan pada bulan Januari-Mei 2021 terhadap 30pasien penderita diabetes melitus di Puskesmas Tanjunganom Kabupaten Nganjuk yang terdiri atas 15 orangperokok aktif dan 15 orang perokok pasif. Pemeriksaan kadar kolesterol total dilakukan di LaboratoriumPuskesmas Tanjunganom Kabupaten Nganjuk. Sedangkan pemeriksaan kadar kolesterol HDL dilakukan di UPTLabkesda Kabupaten Nganjuk. Berdasarkan uji statistik independent sampel t-test diperoleh p-value perbedaankadar kolesterol HDL pada perokok aktif dengan perokok pasif penderita diabetes melitus sebesar 0,000 sertaperbedaan kadar kolesterol total pada perokok aktif dengan perokok pasif penderita diabetes melitus sebesar0,012. Sehingga dapat disimpulkan bahwa secara statistik terdapat perbedaan kadar kolesterol HDL dankolesterol total pada perokok aktif dengan perokok pasif yang menderita diabetes melitus di PuskesmasTanjunganom Kabupaten Nganjuk.Kata Kunci : diabetes melitus; kadar kolesterol; perokok aktif; perokok pasif
PERBEDAAN KADAR ASAM URAT PADA LANSIA MENGGUNAKAN METODE POCT (Point Of Care Testing) DENGAN METODE ENZIMATIK KOLORIMETRI DI PUSKESMAS BANGUNSARI KABUPATEN MADIUN Ayu Pramita Desty Ratna; Haryanto Edy; Arifin Syamsul
Analis Kesehatan Sains Vol. 10 No. 2 (2021): Analis Kesehatan Sains
Publisher : Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (477.721 KB)

Abstract

The role of Laboratory examination as a diagnostic of disease is very important, including in determining the amount of uric acid contained in the body. The POCT (Point Of Care Testing) method and the Enzymatic Colorimetric method using a Photometer are several ways that can be done to determine the amount of uric acid content. This study aims to determine the differences in the results of uric acid examination in the elderly using the POCT method with the Enzymatic Colorimetric method at the Bangunsari Health Center, Madiun Regency.This type of research is an analytic observational study with a cross sectional approach. The research population is the elderly who seek treatment at the Bangunsari Health Center, Madiun Regency. The study was carried out in January-May 2021. The research sample consisted of 30 people who were taken by purposive sampling. Research data in the form of primary data obtained directly from the results of laboratory examinations. The data analysis technique used the normality test with the Kolmogorov Smirnov Test and the Independent-Sample T Test.The results showed that the data on uric acid levels in the elderly at the Bangunsari Public Health Center, Dolopo District, Madiun Regency, which was measured using the POCT (Point Of Care Testing) method showed that the mean and standard deviation values ​​were 5,650±1,283 while those measured using the Enzymatic Colorimetric method were 6,340±1,332 indicating that there was an average difference The average results of measuring uric acid levels in the elderly are between those using the POCT (Point Of Care Testing) method and those using the Enzymatic Colorimetric method. Based on the results of the Independent Sample T-test, it is known that the value of Sig. (2-tailed) of 0.046 <0.05, it can be concluded that H0 is rejected and Hi is accepted. That is, there is a significant difference in uric acid levels using the POCT method with the Enzymatic Colorimetric method.
Peanut Sucrosa Agar (PSA) Sebagai Media Modifikasi Candida albicans pada Urine Penderita Diabetes Melitus Retno Sasongkowati; Edy Haryanto; Evy Diah Woelansari
Window of Health : Jurnal Kesehatan Vol 5 No 2 (April 2022)
Publisher : Fakultas Kesehatan Masyarakat Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/woh.v5i02.19

Abstract

Media standar untuk menumbuhkan jamur adalah media Potato Dextrose Agar ( PDA) dan Sabaroud Dextrose Agar (SDA) namun media ini sulit didapat dan harganya relatif mahal, sehingga diperlukan alternatif media pembiakan jamur Candida albicans dengan pemanfaatan bahan alam. Media alternatif dengan pemanfaatan bahan alam yaitu menggunakan kacang tanah (Arachis hypogaea) varietas Talam-1. Penelitian bertujuan untuk mengetahui pengembangan Peanut Sucrose Agar (PSA) sebagai media modifikasi untuk identifikasi Candida albicans pada urin penderita diabetes melitus. Jenis penelitian ini adalah eksperimental laboratorium dengan rancangan penelitian eksploratoris. Sampel yang digunakan adalah penderita diabetes mellitus yang diambil secara acak. Metode pemeriksaan pemeriksaan glukosa darah puasa, dan pemeriksaan urin rutin. Sedimen urin dibiakan ke media PSA. Hasil pertumbuhan koloni Candida albicans pada media Peanut Sucrosa Agar (PSA) pada Responden Diabetes Melitus yang kadar gula darahnya lebih dari 126 mg/dL dan pemeriksaan lekosit positif didapat hasil yang pertumbuhan lebih dari 150 koloni sebanyak 64.7% dan pada media Potato Dextrosa Agar (PDA) sebesar 70.5%. Sedangkan pada media PSA yang kurang dari 150 koloni sebesar 35.3% dan media PDA sebesar 29.5%.Tumbuhnya jamur Candida albicans pada media Peanut Sucrosa Agar (PSA) menunjukkan mampu sebagai media modifikasi dengan terlihat jumlah koloni yang tumbuh lebih dari 150 koloni sebesar 64.7%. Berdasarkan uji statistik T-Test Paired menghasilkan nilai sig p = 0.077 pada α = 0.05 artinya tidak ada perbedaan pertumbuhan jamur Candida albicans pada media PSA dan media PDA, sehingga disimpulkan bahwa media Peanut Sucrose Agar dapat dipergunakan sebagai Agar media modifikasi untuk pertumbuhan Candida albicans dan disarankan untuk dilakukan penelitian dengan menggunakan jenis jamur yang berbeda.
Co-Authors Anita Dwi Anggraini Arifin Syamsul Ayu Pramita Desty Ratna Ayu Puspitasari Dian Anggraini Endah Astuti, Sri Sulami Evy Diah Woelansari Handayati, Anik Krihariyani, Dwi Retno Sasongkowati Retno Sasongkowati Retno Sasongkowati Sadgassusi Hera Samudra Syamsul Arifin Syamsul Arifin Syamsul Arifin Wisnu Istanto