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All Journal JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Journal Of Vocational Health Studies Jurnal Media Analis Kesehatan Manuju : Malahayati Nursing Journal Journal of Health Sciences 2-TRIK: TUNAS-TUNAS RISET KESEHATAN INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Poltekita : Jurnal Ilmu Kesehatan Jurnal Ilmiah Tatengkorang JOURNAL OF COMMUNITY MEDICINE AND PUBLIC HEALTH RESEARCH Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) CENDEKIA : Jurnal Ilmu Pengetahuan HEALTHY: Jurnal Inovasi Riset Ilmu Kesehatan Jurnal Pengabdian Kepada Masyarakat Mulawarman Gema Lingkungan Kesehatan Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat Frontiers in Community Service and Empowerment Vitamin: Jurnal Ilmu Kesehatan Umum IPSSJ Jurnal Teknokes
Sasongkowati, Retno
Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Surabaya
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The Effect of Sugar Apple (Annona squamosa L.) Seeds Solution as an Insecticide Against Mortality of Aedes aegypti Mosquito with Liquid Electric Method Amalinda Mega Novasari; Retno Sasongkowati
JURNAL KESEHATAN LINGKUNGAN Vol. 9 No. 2 (2017): JURNAL KESEHATAN LINGKUNGAN
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkl.v9i2.2017.200-208

Abstract

Dengue hemorrhagic fever is a public health problem in Indonesia that the number of DHF cases has been increased. Morbidity number of DHF per 100,000 population at 2011 was 26.67 became 34.3 in 2012. The control of DHF has been attempted, but a lot of synthetic insecticide used has been negatively affected to environment. So, it need the insecticide alternative. The aim of study was to identify the effect of solvent sugar apple (Annona squamosa L.) seeds to the death of Aedes aegypti mosquitoes by electric liquid method. Type of this research was experimental with cross sectional study design as well as the post test only control group design. The study was conducted from February to July 2013 in the Laboratory of Entomology of the Provincial Health Office of East Java. The population of this research was the Aedes aegypti with 560 sample of mosquitoes were divided into five treatments and four repetitions for each treatment. The collection of quantitative data drawn from primary data through observation by calculating the death of Aedes aegypti of each the sugar apple seeds concentration of 10%, 20%, 30%, 40% and 50% for 24 hours. The results showed that the concentration of 50% solvent of sugar apple seeds provide the greatest effect in causing the death of Aedes aegypti mosquitoes as many as 36 died after exposure. It can be concluded that there are effected by giving solvent of sugar apple (Annona squamosa L.) seeds to the death of Aedes aegypti mosquitoes by electric liquid method.
EFEKTIVITAS EKSTRAK BIJI SIRSAK TERHADAP MORTALITAS Pediculus humanus var. Capitis SECARA IN VITRO Ahdiah Imroatul Muflihah; Retno Sasongkowati
Jurnal Media Analis Kesehatan Vol 12, No 1 (2021): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Poltekkes Kemenkes Pakassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/mak.v12i1.2002

Abstract

Pediculosis  capitis  adalah  gangguan  yang  disebabkan  oleh  infestasi  Pediculus humanus  capitis. Biji sirsak dapat berpotensi sebagai pedikulisida. Kandungan senyawa aktif dalam biji sirsak ini adalah Acetogenin, Annonain ,Flavonoid, dan Squamosin. Eksperimen ini bertujuan untuk mengetahui efektivitas ekstrak biji sirsak sebagai pedikulisida. Penelitian ini bersifat eksperimental yang dilakukan pada bulan maret-juni 2017 di Laboratorium Parasitology Poltekkes Kemenkes Surabaya. Penelitian ini menggunakan sampel berupa kutu kepala sebanyak 160 ekor dengan masing masing kelompok 5 ekor kutu kepala yang diberikan perlakuan dengan menyemprotkan ekstrak biji sirsak dengan konsentrasi 10%, 20%, 40%, 60%, 80%, dan 100%. Hasil penelitian ini menunjukkan bahwa konsentrasi paling efektif ialah 100% dalam waktu 10 menit, dan konsentrasi minimum adalah 10% dan 20% dalam waktu 50 menit.
Deteksi Gen Penyandi Resistensi Insektisida Karbamat (Ace-1) pada Nyamuk Aedes Aegypti Metode Pcr Revi Zakiyatul Maftukhah; Retno Sasongkowati; Wisnu Istanto; Anita Dwi Anggraini
Malahayati Nursing Journal Vol 4, No 10 (2022): Volume 4 Nomor 10 2022
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/mnj.v4i10.7571

Abstract

ABSTRACT Dengue Hemorrhagic Fever (DHF) is a high endemic problems, especially in the tropics. Effort to prepare for the surge in cases, it is necessary to develop in molecular level, detection of the Ace-1 gene can be used as an important indication of the resistance Aedes aegypti to carbamate insecticides. Purpose to detect the presence or absence of the Ace-1 gene of Aedes aegypti against carbamate insecticides using the PCR method. Methods descriptive quantitative analytic method of observational data. Treatments in experiment consisted of four test bottles and one control bottle. The results that appear on the detection of Ace-1 gene using RT-PCR are CT values. Results showed that from  4 existing samples, 2 of them were positive with a value, sample 1 (A01) had a CT value of 1.00, sample 4 (A04) had a CT value of 4.42 while other 2 were negative which was indicated by the emergence of N/A which means Not Available or not available primary gene in target gene. Conclusion percentage obtained was 50% of samples with Ace-1 gene detected and 50% of other samples not detecting Ace-1 gene. Keywords: Aedes aegypti, Ace-1, CT value  ABSTRAK Demam Berdarah Dengue (DBD) menjadi salah satu masalah endemis yang cukup tinggi khususnya di daerah tropis. Sebagai upaya kesiapsiagaan terhadap lonjakan kasus yang terjadi perlu dikembangkan ke tingkat molekuler yaitu deteksi gen Ace-1 yang dapat dijadikan sebagai indikasi penting dari resistensi nyamuk Aedes aegypti terhadap jenis insektisida golongan karbamat. Tujuan untuk mendeteksi ada tidaknya gen Ace-1 nyamuk Aedes aegypti terhadap insektisida karbamat menggunakan metode PCR. Metode Penelitian deskriptif kuantitatif metode analitik data observasi. Banyaknya perlakuan dari penelitian ini terdiri dari empat botol uji dan satu botol kontrol. Hasil yang muncul pada deteksi gen Ace-1 menggunakan RT-PCR berupa nilai CT. Hasil menunjukkan dari 4 sampel yang ada diperoleh hasil 2 diantaranya positif dengan nilai yaitu pada sampel 1 (A01) mempunyai nilai CT sebesar 1,00, pada sampel 4 (A04) mempunyai nilai CT sebesar 4,42 sedangkan 2 lainnya negatif yang ditandai dengan munculnya N/A yang berarti Not Available atau tidak tersedia gen primer pada gen target. Kesimpulan presentase yang diperoleh sebesar 50% sampel yang terdeteksi adanya gen Ace-1 dan 50% sampel lainnya tidak terdeteksi adanya gen Ace-. Kata kunci: Aedes aegypti, Ace-1, nilai CT
Uji Efektivitas Ekstrak Daun Sirih Hijau (Piper Betle L), Daun Sirih Merah (Piper Crocatum) dan Daun Sirih Kuning (Piper Betle) terhadap Mortalitas Larva Nyamuk Aedes Aegypti Heni Nuril Arfiyanti; Diah Titik Mutiarawati; Retno Sasongkowati; Wisnu Isntanto
Malahayati Nursing Journal Vol 4, No 10 (2022): Volume 4 Nomor 10 2022
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/mnj.v4i10.7510

Abstract

ABSTRACT The  Aedes  aegypti  mosquito  is  the  main  vector  of  DHF  (Dengue Hemorrhagic Fever). In the city of Surabaya in 2019, there were 277 cases of dengue fever. Control using chemical insecticides can create resistance for larvae so that other methods can be used, namely herbal insecticides on green betel  leaves,  red  betel  leaves  and  yellow  betel  leaves  which  contain compounds such as essential oils, alkaloids, flavonoids, saponins and tannins which are toxic and can affect the digestive system. and nervous system in Aedes aegypti larvae. The purpose of this study was to determine the effectiveness of green betel leaf extract (Piper betle L), red betel leaf (Piper crocatum) and yellow betel leaf (Piper betle) as natural insecticides against Aedes  aegypti  larvae.  This  research  was  conducted  at  the  Entomology Laboratory of the East Java Provincial Health Office in May 2022. The data collection technique was experimental. In this study there were 5 treatment groups  with  concentrations  of  0.1%,  0.5%  and 1%  and there  were  positive control groups (abate) and negative control groups, each treatment had 5 replications. Each test contained 20 instar III Aedes aegypti larvae. With the duration of exposure for 60, 120, 180, 240, 300, 360, 420, 480, 540, 1440. The results of this study indicate that the greater the concentration and duration of exposure,  the more  mortality of mosquito larvae. The results of the data analysis of this study showed that they were not normally distributed and were not homogeneous, so the Kruskal-Wallis test gave a p value (0.05) indicating the effect of the extract on the concentration. Then proceed to the Post-Hoc test showing different results at each concentration, if p (0.05) then there is no difference, if p (0.05) then there is a difference in each extract concentration on larval mortality. Keywords: Aedes Aegypti, Piper Betle L, Piper Crocatum, Piper Betle, Larva       Mortality  ABSTRAK Nyamuk Aedes aegypti merupakan vektor utama dari penyakit DBD (Demam Berdarah Dengue). Pada kota Surabaya tahun 2019 kasus DBD  dengan jumlah 277 kasus. Pengendalian menggunakan insektisida kimia dapat menjadikan resistensi untuk larva sehingga dapat menggunakan cara lain yaitu insektisida herbal pada daun sirih hijau, sirih merah dan sirih kuning yang memiliki kandungan senyawa seperti minyak atsiri, alkaloid, flavonoid, saponin dan tanin yang bersifat sebagai racun dapat mempengaruhi sistem pencernaan dan sistem saraf pada larva Aedes aegypti. Tujuan penelitian ini untuk mengetahui efektivitas ekstrak daun sirih hijau (Piper betle L), sirih merah (Piper crocatum) dan sirih kuning (Piper betle) sebagai insektisida alami terhadap  larva  Aedes  aegypti.  Penelitian  ini  dilaksanakan  di  LaboratoriumEntomologi Dinas Kesehatan Provinsi Jawa Timur pada bulan Mei 2022. Teknik pengumpulan data secara eksperimental. Penelitian ini terdapat 5 kelompok perlakuan  konsentrasi  0,1%,  0,5%  dan  1%  serta  terdapat  kelompok  kontrol positif (abate) dan kontrol negatif, setiap perlakuan terdapat 5 kali replikasi. Setiap uji berisi 20 larva Aedes aegypti instar III. Dengan lama pemaparan selama 60, 120, 180, 240, 300, 360, 420, 480, 540, 1440. Hasil penelitian ini menunjukkan bahwa semakin besar  konsentrasi dan lama pemaparan maka semakin banyak mortalitas larva nyamuk. Hasil analisa data penelitian ini menunjukkan tidak berdistribusi normal dan tidak homogen sehingga dilakukan uji kruskal-wallis memberikan nilai p (0,05) menunjukkan adanya pengaruh ekstrak pada konsentrasi. Kemudian dilanjutkan ke uji Post-Hoc menunjukkan hasil berbeda – beda pada setiap konsentrasi, apabila p (0,05) maka tidak ada perbedaan, apabila p (0,05) maka adanya perbedaan pada setiap konsentrasi ekstrak terhadap mortalitas larva. Kata Kunci: Aedes Aegypti, Piper Betle L, Piper Crocatum, Piper Betle,       Mortalitas Larva
Efektivitas Berbagai Ekstrak Varietas Daun Sirih sebagai Repelen Daya Tolak terhadap Gigitan Nyamuk Aedes Aegypti Vemy Rahmany Kurniawan; Diah Titik Mutiarawati; Lully Hanni Endarini; Retno Sasongkowati
Malahayati Nursing Journal Vol 4, No 10 (2022): Volume 4 Nomor 10 2022
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/mnj.v4i10.7511

Abstract

ABSTRACT DHF is a disease caused by the dengue virus and is transmitted from person to person through the bite of the Aedes aegypti mosquito. Control efforts can be carried out using natural insecticides from plants, namely betel leaf. Betel leaf contains saponins, flavonoids, tannins, eugenol, kavicol, alkaloids and essential oils. This study aims to determine the effectiveness of green betel leaf extract (Piper betle L), yellow betel leaf (Piper betle) and red betel leaf (Piper crocatum) as repellant for Aedes aegypti mosquitoes. The research was carried out at the Entomology Laboratory of the East Java Provincial Health Office in April 2022. The data collection technique was carried out experimentally. Preparation of spray concentration 10%, 15%, 17%, 20% was sprayed on the back of the hand exposed to 25 female Aedes aegypti mosquitoes, the exposure was carried out for 5 minutes within 6 hours with every 1 hour the hand was reinserted. This research was conducted with 4 times of replication. The results showed that the highest percentage of repelling repellant occurred at a concentration of 20%, namely in green betel (Piper betle L.) by 95%, yellow betel (Piper betle) by 94%, and red betel (Piper crocatum) by 91%. The results of the study were analysis of alternative test data, namely Kruskall Wallis because the data were not homogeneously distributed, then continued with the Post Hoc test to find out which were the significant differences between the treatment groups. The value of the Kruskall Wallis test was obtained, the value of p (0.05) was Sig. 0.000 which means there is a difference in repulsion to the Aedes aegypti mosquito. Keywords:  Piper betle L, Piper betle, Piper crocatum, Aedes aegypti, Repellant                                               ABSTRAK DBD adalah penyakit yang disebabkan virus dengue dan ditularkan dari orang ke orang melalui gigitan nyamuk Aedes aegypti. Upaya pengendalian dapat dilakukan menggunakan insektisida alami dari tanaman yaitu daun sirih. Daun sirih mengandung senyawa saponin, flavonoid, tanin, eugenol, kavicol, alkaloid dan minyak atsiri. Penelitian ini bertujuan mengetahui efektivitas ekstrak daun sirih hijau (Piper betle L), sirih kuning (Piper betle) dan sirih merah (Piper crocatum) sebagai repelen daya tolak nyamuk Aedes aegypti. Penelitian dilaksanakan di Laboratorium Entomologi Dinas Kesehatan Provinsi Jawa Timur pada bulan April 2022. Teknik pengumpulan data yang dilakukan secara eksperimental. Sediaan spray konsentrasi 10%,15%,17%,20% disemprotkan pada punggung tangan yang dipaparkan 25 ekor nyamuk betina Aedes aegypti,  pemaparan dilakukan selama 5 menit dalam waktu 6 jam dengan setiap 1 jam sekali tangan dimasukkan kembali. Penelitian ini dilakukan dengan 4 kali replikasi. Hasil penelitian didapatkan persentase repelen daya tolak tertinggi terjadi pada konsentrasi 20% yaitu pada sirih hijau (Piper betle L.) sebesar 95%, sirih kuning  (Piper betle) sebesar 94%, dan sirih merah (Piper crocatum) sebesar 91%. Hasil penelitian dilakukan analisa data uji alternatif yaitu Kruskall wallis karena data tidak berdistribusi homogen kemudian dilanjutkan dengan uji Post Hoc untuk mengetahui mana saja perbedaan yang signifikan antar kelompok perlakuan. Didapatkan nilai uji Kruskall wallis nilai p (0,05) yaitu Sig. 0,000 yang mengartikan adanya perbedaan daya tolak terhadap nyamuk Aedes aegypti. Kata  Kunci:  Piper betle L, Piper betle, Piper crocatum, Aedes aegypti, Repelen
STUDI IN SILICO SIFAT FARMAKOKINETIK, TOKSISITAS, DAN AKTIVITAS IMUNOMODULATOR BRAZILEIN KAYU SECANG TERHADAP ENZIM 3-CHYMOTRYPSIN-LIKE CYSTEINE PROTEASE CORONAVIRUS Dwi Krihariyani Dwi; Retno Sasongkowati; Edy Haryanto
Journal of Indonesian Medical Laboratory and Science Vol 1 No 1: Oktober 2020
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v1i1.14

Abstract

The 3-chymotrypsin-like cysteine ??protease (3CLpro) is an enzyme that control replication of the coronavirus life cycle. The 3CLpro is a biological target that will interact with drugs. Brazilein is a major compound found in secang wood that used as an immunomodulator. This study aims to predict the immunomodulatory activity of the brazilein against the 3CLpro using in silico method, with curcumin as comparative compound. In silico test was used to predict immunomodulatory activity by docking using the Molegro Virtual Docke. Receptors used are 3CLpro, PDB code: 6M2N, with ligand 3WL_401[C]. pkCSM online program used to predict pharmacokinetic properties (ADME) and toxicity from brazilein and curcumin. Data was analyzed by comparing the docking bond energy between brazilein, ligands and curcumin at the target receptor. The lower the ligand bond energy, the more stable the bond is formed. The results of the in silico test showed that the ligand bond energy = -83,1153kcal/mol, brazilein = -82.0583kcal/mol, and curcumin = -115.852kcal/mol. Brazilein has lower potential as an immunomodulator than ligands and curcumin. The results of the in silico test using the pkCSM online program showed that the brazilein compound had good pharmacokinetic properties and low toxicity.
Deteksi Jamur Candida albicans Pada Urine Penderita Infeksi Saluran Kemih Menggunakan Metode RT-PCR Retno Sasongkowati; Anita Dwi Anggraini; Dellanis Arina Putri
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 2 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i2.14409

Abstract

                                                        ABSTRAKCandida albicans merupakan anggota flora normal yang terdapat pada selaput mukosa,  saluran pencernaan, saluran pernafasan, uretra, vagina, kulit dan kuku tangan dan kaki. Candida albicans dapat tumbuh pada berbagai macam pH, tetapi umumnya pertumbuhan akan lebih baik pada pH antara 4,5-6,5. Candida albicans dapat tumbuh dalam perbenihan pada suhu 28ºC – 37ºC. Jenis penelitian yang digunakan merupakan penelitian deskriptif kuantitatif. Penelitian ini bertujuan untuk untuk mengetahui adanya jamur Candida albicans pada urine penderita infeksi saluran kemih menggunakan metode Real Time PCR. Populasi dalam penelitian penderita infeksi saluran kemih. Infeksi saluran kemih merupakan infeksi keadaan klinis akibat berkembangbiaknya mikroorganisme yang menyebabkan inflamasi pada saluran kemih. Penelitian dilaksanakan di Laboratorium Parasitologi dan Laboratorium Biologi Molekuler pada bulan April 2022 hingga Mei 2022. Pada penelitian ini dilakukan beberapa tahapan pemeriksaan seperti isolasi kultur pada media Sabaroud Dextrose Agar, pemeriksaan makroskopis dan mikroskopis, pembuatan suspensi sel, uji ekstraksi, uji kemurnian DNA, optimasi dan pemeriksaan Real Time PCR dengan menunjukkan hasil berupa CT. Hasil penelitian menunjukkan pada pemeriksaan Real Time PCR ini didapatkan sejumlah 2 sampel dengan persentase 22,2% pada kode sampel S7 dengan nilai CT 28,50 dan kode sampel S9 dengan nilai CT 23,19 dan hasil negatif sejumlah 7 sampel dengan persentase 77,8%. Adapun faktor penyebab yang dapat mengakibatkan keberadaan jamur Candida albicans pada urine penderita infeksi saluran kemih seperti personal hygiene, penderita diabetes mellitus, alat kontrasepsi dan penggunaan antiseptik. 
Utilization of Green Beans Vima 1 and Local as an Alternative Media Substitute Sabouraud Dextrose Agar (SDA) in the Growth of Trichophyton Rubrum Widyanur; Retno Sasongkowati; Suliati
Jurnal Ilmiah Kesehatan Vol 16 No 01 (2023): Jurnal Ilmiah Kesehatan (Journal of Health Science) 
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/jhs.v16i01.3181

Abstract

Green beans are cultivated plants and crops with nutrients from vegetable protein. Varieties of mung beans vary, including Vima 1 and local. The fungus Trichophyton rubrum generates dermatophytosis. This research explored green beans and local Vima 1 as an alternative medium in the growth of Trichophyton rubrum fungus. The type of research used by laboratory experiments was carried out at the STIKes Hutama Abdi Husada Tulungagung Microbiology Laboratory in March - April 2022. SDA media and alternative media green beans Vima 1 and local variations of the mass of 6 grams and 7 grams inoculated Trichophyton rubrum fungus using a single dot method with repetition as much as five times. The results showed that Trichophyton rubrum fungus grows optimally on alternative media green beans Vima 1 variation of 7 grams of mass that grows colonies on the fifth day with an average final diameter of 14 mm. While alternative media green beans Vima 1 variation mass 6 grams 11 mm, SDA media 11.65 mm, alternative media local green beans variation mass 6 grams 7.5 mm, and variation mass 7 grams 11.5 mm. The result of independent statistical tests was a p-value < 0.00, which shows significant differences in each type of media. This study result concluded that green beans Vima 1 and local could be used as an alternative medium to replace the SDA media in the growth of Trichophyton rubrum fungus.
Pendampingan dan Peningkatan Pengetahuan Manfaat Infusa Bawang Putih pada Masyarakat dalam Mencegah dan Mengatasi Hipertensi pada Masyarakat di Desa Ketetang Kecamatan Kwanyar Kabupaten Bangkalan Edy Haryanto; Sri Sulami Endah Astuti; Retno Sasongkowati; Anita Dwi Anggraini
ABDIKESMAS MULAWARMAN : Jurnal Pengabdian Kepada Masyarakat Vol.3 No.1 Mei (2023) : ABDIKESMAS MULAWARMAN
Publisher : Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Hipertensi merupakan penyakit degeneratif yang menjadi masalah serius saat ini. Bahaya hipertensi yang tidak dapat dikendalikan dapat menimbulkan komplikasi yang berbahaya, seperti penyakit jantung koroner, stroke, ginjal dan gangguan penglihatan. Beberapa faktor yang berperan dalam terjadinya hipertensi meliputi faktor mayor yaitu faktor risiko yang tidak dapat dikendalikan dan faktor minor yaitu faktor risiko yang masih dapat dikendalikan. Permasalahan yang terjadi pada masyarakat adalah kurangnya pengetahuan tentang pengendalian penyakit hipertensi serta faktor-faktor yang mempengaruhi kejadian hipertensi dan ketidaktahuan masyarakat dalam memanfaatkan bahan herbal yang ada disekitar untuk dimanfaatkan untuk menurunkan hipertensi yaitu contohnya bawang putih. Kegiatan pengabdian masyarakat yang dilakukan pada warga di Desa Ketetang dusun kwalas berhasil dilaksanakan dan diikuti sebanyak 31 orang yang mengikuti pemeriksaan tekanan darah, kegiatan pelatihan dan penyuluhan pembuatan dan pemanfaatan infusa bawang. Masalah kesehatan yang dikeluhkan oleh warga mampu diatasi oleh tim pengabdian masyarakat dengan memberikan edusi pelatihan dan penyuluhan pembuatan infusa bawang putih sebagai terapi dari herbal untuk menurunkan tekanan darah. Selama kegiatan berlangsung peserta sangat antusias, hal ini dapat dilihat pada keaktifan peserta mengikuti dari awal sampai akhir.
DETEKSI E. coli PENGHASIL Extended Spectrum Beta-Lactamase (ESBL) MENGGUNAKAN METODE PCR PADA SAMPEL AIR BERSIH Lella Rahmawati; Retno Sasongkowati; Anita Anggraini; Deddy Adam
Gema Lingkungan Kesehatan Vol. 20 No. 2 (2022): GEMA Lingkungan Kesehatan
Publisher : Poltekkes Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (111.135 KB) | DOI: 10.36568/gelinkes.v20i2.35

Abstract

Escherichia coli is a normal flora in the human body that plays an important role in the digestive process. The presence of Escherichia coli ESBL (Extended Spectrum Beta-Lactamase) bacteria in clean water samples indicates environmental pollution originating from feces. The aquatic environment is the most influential ecosystem in human life. These contaminated ecosystems provide optimal conditions for antibiotic-resistant bacteria from various sources to mix and transfer their resistance genes to clinically important bacteria for the development of human pathogens with novel resistance mechanisms. This study aims to determine the percentage of ESBL-producing E. coli bacteria in clean water samples. This study uses a descriptive observational design. The total sampling is 195 clean water samples. After going through screening in the form of membrane filter tests, KIA, and IMVIC, 25 isolates were found which were declared as E. coli. Analysis of the ESBL-producing E. coli gene using conventional PCR. Analysis of the Molecular Weight value in the sample using the GelAnalyzer 19.1 application. Based on these calculations, it was found that samples of E. coli isolates containing the ESBL gene were 24% of the 25 E. coli isolates. This percentage indicates that there is a large enough contamination in clean water. The results of this study indicate a potential risk to public health. The participation of health workers, government and the community in maintaining sanitary hygiene is needed to avoid greater contamination of antibiotic-resistant bacteria.
Co-Authors ., Pestariati AHDIAH IMROATUL MUFLIHAH Amalinda Mega Novasari Amelia, Noer Anggraini, Anita Dwi Anita Anggraini Anita Dwi Anggraini Anita Dwi Anggraini Ariana, Novi Astuti Setiyani Bambang Hadi Sugito Briansky, Sabira Alvayogi Choirudin, Nadia Pramesthy Choirudin Cholidah, Ema Zahiroh Nur Christiyaningsih, Juliana Darjati Deddy Adam Dedi Kurniawan Dellanis Arina Putri Demes Nurmayanti Edy Haryanto Edy Haryanto Endah Astuti, Sri Sulami Endarini, Lully Hani Endarini, Lully Hanni Ersanto, Novi Evi Yunita N Evy Diah Woelansari Hadi Suryono Haqqi, Faishol Heni Nuril Arfiyanti Indah Lestari, Indah Ira Puspita Sari Isfentiani, Dina Isnaini, Dian Istanto, Wisnu Juliana Christianingsih Juliana Christyaningsih Kartika Rahayuningsih, Christ Khambali, Khambali Kiaonarni OW Krihariyani, Dwi Kristiningsih, Elly Kusumaningrum, Debby Kuswanto, Muhammad Lella Rahmawati Lembunai Tat Alberta, Lembunai Tat Luthfi Rusyadi M. Zakarahman Makruf, Anang Mamik Mamik Maudita, Nauril Minarti Minarti Mitra Putri Mandiri Museyaroh, Museyaroh Mutiarawati, Diah Titik N. S. Widodo Novita, Devy Nur Hatijah Pengge, Nuning Marina Prisma Anjarlena Purnomowati, Anik Puspita Dewi, Teresia Retna Puspitasari, Erlita Revi Zakiyatul Maftukhah Rusmania, Liya Safrudin, Samsul Saktiyani, Fisari Salis Irawati Siagian, Hotmaida Siti Alfiah Sugihani Suhariyadi Suhariyadi Suliati Suliati Suliati Suliati Suliati Sumasto, Hery Toni Watoyani Trisnania, Iid Dani Vemy Rahmany Kurniawan Widyanur Wisnu Isntanto Wisnu Istanto Wisnu Istanto Yusianti Silviani