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Phytochemical Screening and In Vitro Antibacterial Activity of Green Tea (Camellia Sinensis L) Extract Against Staphylococcus Epidermidis Hardian Bimanto; Yuyun Dwi Wahyuni; Diah Titik Mutiarawati; Lully Hanni Endarini
Health Notions Vol 4, No 8 (2020): August
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn40805

Abstract

This study aimed to determine phytochemical screening and ethanol extract activity of the green leaves against Staphylococcus epidermidis. The microbial activity of the ethanol extract was carried out using the diffusion and dilution method with concentrations used of 1.5%, 2.5%, 3.5%, 4.5%, and 5.5% accompanied by three repetitions. The results showed that the ethanol extract of green tea leaves contained alkaloids, saponins, steroids or triterpenoids, flavonoids, polyphenols, and tannins and had the largest inhibition zone diameter of 30mm at a concentration of 4.5%. KHM (Minimum Inhibitory Content) at a level of 4.5% and KBM (Minimum Kill Rate) at a concentration of 5.5%. Analysis of the data with the normality test then proceed with the Kruskal Wallis test obtained a value of P = 0.008. With the value P
Correlation of Fasting Blood Glucose Levels and HbA1c with Body Mass Index in Type 2 Diabetes Mellitus Patients Anik Handayati; Lully Hanni Endarini; Yuni Eka Cahyana
Health Notions Vol 6, No 4 (2022): April
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn60404

Abstract

Excess body mass index is at greater risk of developing diabetes mellitus than other diseases. Monitoring blood glucose includes through examination of fasting blood glucose levels and HbA1c. This study aims to find out the relationship of fasting blood glucose levels and HbA1c with Body Mass Index in type 2 diabetes mellitus patients. This study was an observational analytical study with a cross sectional design. The research was conducted at Prima Surabaya Main Clinic in January to June 2021. The sample was a type 2 diabetes mellitus patient who conducted fasting blood glucose examination, HbA1c and Body Mass Index measurement at Prima Surabaya Main Clinic as many as 40 people, were taken purposive sampling. The results of this study found that the average fasting blood glucose level of patients with type 2 diabetes mellitus was 172.6 mg / dL and HbA1c was 7.76%. The average Body Mass Index was 24.97. Pearson correlation test results obtained significant Body Mass Index with fasting blood glucose levels p = 0.150 and HbA1c with Body Mass Index p = 0.295, which means there is no relationship between fasting blood glucose levels and HbA1c with body mass index in type 2 diabetes mellitus patients.  Keywords: fasting blood glucose; HbA1c; Body Mass Index; auto analyzer
Rendemen dan Skrining Fitokimia Ekstrak Etanol Daun Teh Hijau (Camellia sinensis L) Lully Hanni Endarini
2-TRIK: TUNAS-TUNAS RISET KESEHATAN Vol 11, No 2 (2021): Mei 2021
Publisher : FORUM ILMIAH KESEHATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/2trik11210

Abstract

The purpose of this study was to determine the yield and phytochemical screening of the ethanol extract of green tea leaves (Camellia sinensis L). This type of research was a descriptive study, the sample was selected by random sampling technique. The yield of the extract produced from this study was 96.699% and the results of phytochemical screening showed that the extract contained alkaloid compounds, saponins, steroids or triterpenoids, flavonoids, polyphenols and tannins. Keywords: yield; camellia sinensis l; phytochemical screening ABSTRAK Tujuan penelitian ini adalah untuk mengetahui hasil rendemen dan skrining fitokimia ekstrak etanol daun teh hijau (Camellia sinensis L). Jenis penelitian yang digunakan adalah studi deskriptif, sampel dipilih dengan teknik random sampling. Hasil rendemen ekstrak yang dihasilkan dari penelitian ini adalah sebesar 96,699% dan hasil skrining fitokimia menunjukkan bahwa ekstrak mengandung senyawa alkaloid, saponin, steroid atau triterpenoid, flavonoid, polifenol dan tanin. Kata kunci: rendemen; camellia sinensis l; skrining fitokimia
Aktivitas Antibakteri Ekstrak Daun Teh Hijau (Camellia sinensis) Terhadap Pertumbuhan Bakteri Escherichia coli Lully Hanni Endarini; Diah Titik Mutiarawati
2-TRIK: TUNAS-TUNAS RISET KESEHATAN Vol 11, No 3 (2021): Agustus 2021
Publisher : FORUM ILMIAH KESEHATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/2trik11308

Abstract

Green tea leaves (C. Sinensis) can be used as a treatment for bacterial infections because they contain antibacterial active compounds. One of the bacteria that causes diarrhea is Escherichia Coli. Green tea (Camellia sinensis) is known as a plant that has antibacterial activity. The purpose of this study was to determine the optimization of the inhibitory power of green tea leaf extract (Camellia sinensis) against the growth of Escherichia coli bacteria. This research is an experimental laboratory conducted at the Bacteriology Laboratory, Department of Medical Laboratory Technology, Surabaya April 2021. This study uses the disk diffusion method Kirby Bauer, namely Mueller Hinton Agar (MHA) media which has been planted with Escherichia coli bacteria in disks that have been soaked in green tea leaf extract (Camellia sinensis) at concentrations of 25%, 50%, 75%, and 100% and then incubated for 24 hours in an incubator. From the results of the study, it can be concluded that green tea leaf extract (Camellia sinensis) has antibacterial activity. The optimal results in this study were Escherichia coli bacteria at a concentration of 25% with an average diameter of 10.25 mm This is because the main content of green tea leaves (Camellia sinensis) is catechins and alkaloids as strong antibacterials.Keywords: antibacterial; camellia sinensis; Escherichia coliABSTRAK Daun teh hijau (C.sinensis) dapat digunakan sebagai pengobatan infeksi bakteri karena mengandung senyawa aktif antibakteri. Salah satu bakteri penyebab diare adalah Escherichia Coli . Teh hijau (Camellia sinensis) dikenal sebagai tanaman yang mempunyai aktivitas antibakteri. Tujuan penelitian ini adalah untuk mengetahui optimalisasi daya hambat ekstrak daun teh hijau  (Camellia sinensis) terhadap pertumbuhan bakteri Escherichia coli.Penelitian ini bersifat eksperimental laboratorium yang dilakukan di Laboratorium Bakteriologi Jurusan Teknologi Laboratorium Medik Surabaya April 2021. Penelitian ini menggunakan disk diffusion metode Kirby Bauer, yaitu media Mueller Hinton Agar (MHA) yang telah ditanami biakan bakteri Escherichia coli  di dalam disk yang sudah di rendam ekstrak daun teh hijau (Camellia sinensis) konsentrasi 25%, 50%, 75%, dan 100% lalu diinkubasi selama 24 jam pada inkubator. Dari hasil penelitian dapat disimpulkan bahwa esktrak daun teh hijau (Camellia sinensis) mempunyai aktivitas antibakteri. Hasil optimal pada penilitian ini bakteri Escherichia coli pada konsentrasi 25% dengan rata-rata diameter 10,25 mm Hal tersebut dikarenakan kandungan utama daun teh hijau (Camellia sinensis) katekin dan alkaloid sebagai antibakteri yang kuatKata kunci: antibakteri; camellia sinensis; Escherichia coli
Efektivitas Berbagai Ekstrak Varietas Daun Sirih sebagai Repelen Daya Tolak terhadap Gigitan Nyamuk Aedes Aegypti Vemy Rahmany Kurniawan; Diah Titik Mutiarawati; Lully Hanni Endarini; Retno Sasongkowati
Malahayati Nursing Journal Vol 4, No 10 (2022): Volume 4 Nomor 10 2022
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/mnj.v4i10.7511

Abstract

ABSTRACT DHF is a disease caused by the dengue virus and is transmitted from person to person through the bite of the Aedes aegypti mosquito. Control efforts can be carried out using natural insecticides from plants, namely betel leaf. Betel leaf contains saponins, flavonoids, tannins, eugenol, kavicol, alkaloids and essential oils. This study aims to determine the effectiveness of green betel leaf extract (Piper betle L), yellow betel leaf (Piper betle) and red betel leaf (Piper crocatum) as repellant for Aedes aegypti mosquitoes. The research was carried out at the Entomology Laboratory of the East Java Provincial Health Office in April 2022. The data collection technique was carried out experimentally. Preparation of spray concentration 10%, 15%, 17%, 20% was sprayed on the back of the hand exposed to 25 female Aedes aegypti mosquitoes, the exposure was carried out for 5 minutes within 6 hours with every 1 hour the hand was reinserted. This research was conducted with 4 times of replication. The results showed that the highest percentage of repelling repellant occurred at a concentration of 20%, namely in green betel (Piper betle L.) by 95%, yellow betel (Piper betle) by 94%, and red betel (Piper crocatum) by 91%. The results of the study were analysis of alternative test data, namely Kruskall Wallis because the data were not homogeneously distributed, then continued with the Post Hoc test to find out which were the significant differences between the treatment groups. The value of the Kruskall Wallis test was obtained, the value of p < (0.05) was Sig. 0.000 which means there is a difference in repulsion to the Aedes aegypti mosquito. Keywords:  Piper betle L, Piper betle, Piper crocatum, Aedes aegypti, Repellant                                               ABSTRAK DBD adalah penyakit yang disebabkan virus dengue dan ditularkan dari orang ke orang melalui gigitan nyamuk Aedes aegypti. Upaya pengendalian dapat dilakukan menggunakan insektisida alami dari tanaman yaitu daun sirih. Daun sirih mengandung senyawa saponin, flavonoid, tanin, eugenol, kavicol, alkaloid dan minyak atsiri. Penelitian ini bertujuan mengetahui efektivitas ekstrak daun sirih hijau (Piper betle L), sirih kuning (Piper betle) dan sirih merah (Piper crocatum) sebagai repelen daya tolak nyamuk Aedes aegypti. Penelitian dilaksanakan di Laboratorium Entomologi Dinas Kesehatan Provinsi Jawa Timur pada bulan April 2022. Teknik pengumpulan data yang dilakukan secara eksperimental. Sediaan spray konsentrasi 10%,15%,17%,20% disemprotkan pada punggung tangan yang dipaparkan 25 ekor nyamuk betina Aedes aegypti,  pemaparan dilakukan selama 5 menit dalam waktu 6 jam dengan setiap 1 jam sekali tangan dimasukkan kembali. Penelitian ini dilakukan dengan 4 kali replikasi. Hasil penelitian didapatkan persentase repelen daya tolak tertinggi terjadi pada konsentrasi 20% yaitu pada sirih hijau (Piper betle L.) sebesar 95%, sirih kuning  (Piper betle) sebesar 94%, dan sirih merah (Piper crocatum) sebesar 91%. Hasil penelitian dilakukan analisa data uji alternatif yaitu Kruskall wallis karena data tidak berdistribusi homogen kemudian dilanjutkan dengan uji Post Hoc untuk mengetahui mana saja perbedaan yang signifikan antar kelompok perlakuan. Didapatkan nilai uji Kruskall wallis nilai p < (0,05) yaitu Sig. 0,000 yang mengartikan adanya perbedaan daya tolak terhadap nyamuk Aedes aegypti. Kata  Kunci:  Piper betle L, Piper betle, Piper crocatum, Aedes aegypti, Repelen
Correlation Air CO Level with HbCo Level, Hemoglobin, and Blood Pressure to Street Vendors in The Gading Fajar Sidoarjo Street Vendor Area Indira Ayuni Sri Pramasti; Ayu Puspitasari; Lully Hanni Endarini; Christ Kartika Rahayuningsih
Jurnal Teknokes Vol 15 No 4 (2022): December
Publisher : Jurusan Teknik Elektromedik, POLTEKKES KEMENKES Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/teknokes.v15i4.504

Abstract

Motor vehicles emit exhaust gas emissions that produce pollutants, one of the compounds contained is carbon monoxide (CO). CO may cause intoxication in humans if exposed for a long time especially street vendors because the work area is filled with vehicle fumes containing CO gas. High CO levels in the air indicate HbCO levels to increase. The increased of HbCO levels will cause abnormal hemoglobin levels and induce vasoconstriction which makes blood pressure increase. The purpose of this study was to prove the correlation between CO levels in the air with HbCO, hemoglobin, and blood pressure levels to street vendors in the Gading Fajar Sidoarjo Street Vendor Area. This research uses the Conway diffusion cell method and is an analytic observational study with a cross sectional design implemented at the Surabaya Health Laboratory Center and the Hematology Laboratory of Medical Laboratory Technology Department Poltekkes Kemenkes Surabaya in March 2022. Blood tests was carried out on 25 street vendors using random sampling technique. The results showed that average air CO level was 11.0 ppm and 13.3 ppm; mean HbCO levels 0.87%; mean hemoglobin levels 14.4 g/dL; mean systolic blood pressure levels 128 mmHg; and the mean of diastolic blood pressure levels 80 mmHg. The data were analyzed using SPSS. The outcome of normality test showed that the data was not normally distributed. Statistical tests was performed using Spearman correlation. The conclusion showed there was correlation between air CO level with HbCO level, hemoglobin level, and blood pressure level on street vendors in the Gading Fajar Sidoarjo Street Vendor Area. If CO levels in the air increase, then HbCO levels and blood pressure also increase. Street vendors are expected to always use Personal Protective Equipment (PPE) such as mouth masks when working to reduce the risk of exposure to CO gas and doing a healthy lifestyle as a preventive effort to avoid health problems.
Pertumbuhan Bakteri Bacillus Cereus dan Salmonella Typhi pada Ekstrak Mentimun (Cucumis Sativus) Lully Hanni Endarini; Diah Titik Mutiarawati; Alda Nugrahini
Jurnal Penelitian Kesehatan SUARA FORIKES Vol 13, No 3 (2022): Juli 2022
Publisher : FORIKES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/sf13317

Abstract

Enteropathy caused by ingestion of food contaminated with bacteria causes morbidity and mortality in many countries. Several bacteria, including Salmonella typhi and Bacillus cereus have been reported to cause food poisoning. Cucumis sativus is useful for humans as antibacterial, hepatoprotective, antioxidant, anthelmintic, antiulcer and anti-inflammatory. Antibacterial test of cucumber ethanol extract was carried out with agar disc-diffusion according to the Kirby Bauer method. The ready test bacterial suspension was then inoculated and put into an incubator at 37°C for 24 hours in an inverted position. After 24 hours, the antibacterial activity around the disc in the test dish was observed by looking at the clear zone around the disc. The results showed that the inhibition zone formed on Salmonella typhi bacteria with concentrations of 30%, 50% and 70% had an average of 11.4 mm, 15 mm and 18.5 mm. Meanwhile, Bacillus cereus bacteria have an average inhibition zone of 7 mm, 18.7 mm and 19.1 mm. The results of the one way ANOVA test obtained a sig value. p<0.05 which proves the presence of antibacterial effect in inhibiting the growth of Salmonella typhi and Bacillus cereus.Keywords: cucumber extract; Salmonella typhi; Bacillus cereus; antibacterial ABSTRAK Enteropati yang disebabkan oleh konsumsi makanan yang terkontaminasi bakteri menyebabkan morbiditas dan mortalitas di banyak negara. Beberapa bakteri, termasuk Salmonella typhi dan Bacillus cereus telah dilaporkan menyebabkan keracunan makanan. Cucumis sativus bermanfaat bagi manusia sebagai antibakteri, hepatoprotektif, antioksidan, anthelmintik, antiulkus dan antiinflamasi. Uji antibakteri ekstrak etanol mentimun dilakukan dengan agar disc-diffusion menurut metode Kirby Bauer. Suspensi bakteri uji yang telah siap kemudian diinokulasi dan dimasukan ke dalam inkubator dengan suhu 37OC selama 24 jam dengan posisi terbalik. Setelah 24 jam, aktivitas antibakteri disekitar cakram dalam cawan uji diamati dengan melihat adanya clear zone di sekitar cakram. Hasil penelitian menunjukkan zona hambat terbentuk pada bakteri Salmonella typhi dengan konsentrasi 30%, 50% dan 70% memiliki rerata 11,4 mm, 15 mm dan 18,5 mm. Sedangkan pada bakteri Bacillus cereus memiliki rerata zona hambat 7 mm, 18,7 mm dan 19,1 mm. Hasil uji one way ANOVA didapatkan nilai sig. p<0,05 yang membuktikan terdapatnya efek antibakteri dalam menghambat pertumbuhan Salmonella typhi dan Bacillus cereus.Kata kunci: ekstrak mentimun; Salmonella typhi; Bacillus cereus; antibakteri
Stabilitas Serum Kontrol Liofilisat Buatan Sendiri Setelah Rekonstitusi Terhadap Kadar Kolesterol dan Trigliserida yang Disimpan dalam Freezer Suhu (-2°C) sampai (-4°C) dan (-20°C) Nada Nabilah Wulandari; Anik Handayati; Lully Hanni Endarini
Jurnal Penelitian Kesehatan SUARA FORIKES Vol 14, No 1 (2023): Januari 2023
Publisher : FORIKES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/sf14114

Abstract

Lyophilisate control serum is a freeze-dried control serum. Control serum must be stable and can be examined for a long time in order to assess the performance of a laboratory, including the quality of tools and reagents. Cholesterol is a fat produced by the liver that causes heart disease in excess levels. Triglycerides are a form of fat storage in the body which functions as a source of energy. This study aimed to determine the stability of homemade lyophilisate control serum after reconstitution on cholesterol and triglyceride levels stored in the freezer at (-2°C) to (-4°C) and (-20°C). This study applied a one group post-test design. Samples were examined for 8 weeks with venous blood test material for lyophilisate control serum. The results of the analysis showed that cholesterol levels with storage for 8 weeks at temperatures (-2°C) to (-4°C) and (-20°C) were stable with an average within the range of ±2SD and CV between 1-6 %. Triglyceride levels with storage for 8 weeks at (-2°C) to (-4°C) were stable with an average within the range of ±2SD and a CV between 2-7%. R-square on the storage time of 8 weeks of lyophilisate control sera after reconstitution showed that storage time had a 16.35% effect on cholesterol stability at temperature (-2°C) to (-4°C), 0% on cholesterol stability at temperature (-20°C) ), 41.7% on triglyceride stability at temperature (-2°C) to (-4°C), and 32.52% on triglyceride stability at temperature (-20°C). The conclusion of this study is that the control sera of homemade lyophilisate after reconstitution with cholesterol and triglyceride parameters stored for 8 weeks is still stable and has a standard deviation within limits.Keywords: lyophilisate serum; reconstitution; cholesterol; triglycerides ABSTRAK Serum kontrol liofilisat merupakan serum kontrol bentuk beku kering. Serum kontrol harus stabil dan dapat diperiksa dalam jangka waktu yang lama agar dapat menilai kinerja suatu laboratorium, termasuk kualitas alat dan reagensia. Kolesterol merupakan lemak yang diproduksi oleh hati yang menyebabkan penyakit jantung dalam kadar berlebih. Trigliserida merupakan bentuk simpanan lemak dalam tubuh yang berfungsi sebagai sumber energi. Penelitian ini bertujuan untuk mengetahui stabilitas serum kontrol liofilisat buatan sendiri setelah rekonstitusi terhadap kadar kolesterol dan trigliserida yang disimpan dalam freezer suhu (-2°C) sampai (-4°C) dan (-20°C). Penelitian ini menerapkan one group post-test design. Sampel diperiksa selama 8 minggu dengan bahan uji darah vena untuk serum kontrol liofilisat. Hasil analisis menunjukkan bahwa kadar kolesterol dengan penyimpanan selama 8 minggu pada suhu (-2°C) sampai (-4°C) dan (-20°C) adalah stabil dengan rata-rata dalam batas rentang ±2SD dan CV antara 1-6%. Kadar trigliserida dengan penyimpanan selama 8 minggu pada suhu (-2°C) sampai (-4°C) adalah stabil dengan rata-rata dalam batas rentang ±2SD dan CV antara 2-7%. R-square pada lama penyimpanan 8 minggu serum kontrol liofilisat setelah rekonstitusi menunjukkan lama penyimpanan berpengaruh 16,35% terhadap stabilitas kolesterol suhu (-2°C) sampai (-4°C), 0% terhadap stabilitas kolesterol suhu (-20°C), 41,7% terhadap stabilitas trigliserida suhu (-2°C) sampai (-4°C), dan 32,52% terhadap stabilitas trigliserida suhu (-20°C). Kesimpulan dari penelitian ini adalah bahwa serum kontrol liofilisat buatan sendiri setelah rekonstitusi dengan parameter kolesterol dan trigliserida yang disimpan selama 8 minggu masih stabil dan mempunyai standar deviasi dalam batas.Kata kunci: serum liofilisat; rekontitusi; kolesterol; trigliserida
Peningkatan Kemandirian Siswa dalam Menerapkan Pola Hidup Bersih dan Sehat untuk Mencegah dan Mengurangi Protozoa Usus pada Anak SDN Ngingas Diah Titik Mutiarawati; Lully Hanni Endarini; Era Fitria Yunita; Anita Dwi Anggraini
ABDIKESMAS MULAWARMAN : Jurnal Pengabdian Kepada Masyarakat Vol.3 No.1 Mei (2023) : ABDIKESMAS MULAWARMAN
Publisher : Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Infeksi protozoa usus masih menjadi salah satu permasalahan di dunia, terutama di negara-negara berkembang dan beriklim tropis. Angka insidensi kasus protozoa usus di Indonesia mencapai 10-18%. Infeksi protozoa usus dapat menyerang anak-anak dan orang dewasa (Deza et al., 2018). Di Negara berkembang tingkat pendidikan masih rendah dan iklim tropis merupakan faktor risiko infeksi protozoa usus. Hasil penelitian Charisma dkk. (2020), menyebutkan bahwa pada pemeriksaan sampel feses menunjukkan sebanyak 20,8% positif adanya kista protozoa usus. Didukung oleh penelitian Simatupang et al., (2013) yang melaporkan sebanyak 40% kasus infeksi didominasi oleh protozoa usus daripada cacing usus (STH) pada anak usia SD di Kedung Cowek, Surabaya. Rendahnya pengetahuan masyarakat terkait penyakit parasit masih menjadi salah satu masalah. Dengan demikian, pemberian edukasi kebersihan personal pada mereka menjadi salah satu solusi untuk menurunkan angka infeksi parasit usus (Fransisca et al., 2015). Dengan demikian, peneliti akanmengidentifikasi keberadaan protozoa usus pada sampel feses yang bertujuan untuk mengetahui prevalensi protozoa usus dan gambaran kebersihan personal pada anak usia sekolah dasar di desa Ngingas Barat, kecamatan Krian kabupaten Sidoarjo. Dari hasil pretes dan postes terdapat terdapat peningkatan para siswa dalam menjawab soal dari 2.16 menjadi 3.66. Soal yang paling sulit dijawab oleh siswa adalah nomor 4 yaitu tentang protozoa. Setelah mendapat materi tentang protozoa usus, para siswa mampu menjawab soal nomor 4 pada postes. Peningkatan pengetahuan tentang protozoa usus sebagai penyebab diare diharapkan dapat mengubah sikap dan tindakan para siswa menjadi lebih baik lagi untuk mencegah terjadinya penyakit tersebut. Satu minggu kemudian setelah diadakan kegiatan penyuluhan para siswa dilakukan pemeriksaan Feses lengkap untuk diperiksa adanya infeksi protozoa. Hasil pemeriksaan laboratorium dinyatakan semua siswa negatif dari infeksi protozoa atau 0%.
ANALISIS KADAR TIMBAL DALAM URIN PETUGAS OPERATOR DAN NONOPERATOR DI KECAMATAN KADEMANGAN KOTA PROBOLINGGO Pratiwi Dewi Ika; Lestari Indah; Endarini Lully Hanni
Analis Kesehatan Sains Vol. 10 No. 1 (2021): Analis Kesehatan Sains
Publisher : Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.474 KB)

Abstract

Gas stations are a source of lead exposure that comes from vehicle gas emissions and petrol fuel vapors. Gas station officers, both operators and nonoperators, have a risk level of lead exposure that can enter the body and then accumulate and excreted through urine by 75-80%. Lead exposure can endanger the health of gas station officers. The purpose of this study was to analyze differences in lead levels in the urine of gas station operators and nonoperators in Kademangan District, Probolinggo City. This research is an observational study with a cross sectional method which was conducted at the Surabaya Health Laboratory Center in October 2020 - June 2021. The sample used was 15 operators and 15 nonoperators who were taken by purposive sampling. The results showed the average value of lead levels in the urine for operators 0.0007213 ppm, indoor nonoperators 0.0006386 ppm and outdoor nonoperators 0.0008263 ppm. The conclusion of this study was that there was no significant difference in lead levels in the urine of gas station operators and nonoperators.