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Morphometric Description of Polistes tepidus from Ternate Island and Obi Island (Wallacea, Indonesia) Wahyu Agung Bintoro; Hari Nugroho; Purwatiningsih
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.19639

Abstract

This study aimed to descriptively compare the morphometric measurements of two Polistes tepidus specimens from Ternate Island and Obi Island preserved in the Museum Zoologicum Bogoriense. The research was conducted by measuring morphometric characters using a Leica Z6 APO microscope and L.A.S. 4.13 software. The observed characters included the head, thorax, wings, and metasoma, which were measured from three viewing angles: frontal, lateral, and dorsal. A total of 33 morphometric characters of the paper wasp were examined, and the mean measurements were analyzed using Microsoft Excel. The results showed that the morphometric measurements of P. tepidus from the two different locations, Ternate Island and Obi Island, exhibited size variation in several body characters. In general, P. tepidus individuals from Ternate Island had larger body sizes than those from Obi Island for most morphometric characters. Habitats with abundant food resources may allow individuals to develop larger body sizes. The morphometric differences between P. tepidus from Ternate and Obi Islands may also be explained by the effects of geographic isolation and the differing ecological conditions of the two islands. The islands of the Wallacea region are known for their high environmental heterogeneity, and insect populations therefore often exhibit morphological differences among regions. These findings highlight the need for broader sampling to further test geographic differentiation.
DHF Prevention Efforts in Sumbersari Jember: Assessment of Aedes aegypti Resistance Status to Deltamethrin Reni Puspitasari; Hidayatur Rohmah; Husnatun Nihayah; Purwatiningsih; Eva Tyas Utami; Susantin Fajariyah; Asmoro Lelono
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.19883

Abstract

This study aims to determine the resistance status of Ae. aegypti to the deltametrin insecticide in Sumbersari Jember Village. A total of 100 2nd generation female Ae. aegypti were tested using the CDC bottle bioassay with a deltamethrin dose of 10 μg/ml and a diagnostic time of 30 minutes. Molecular detection using AS-PCR was also performed to detect kdr mutations at position 1016 of the VGSC gene. Mortality data was calculated using Ms.Excel with a confidence level of 95%. The molecular wight of the PCR product is calculated using a gel analyzer application.  The results of the CDC bottle bioassay show that the Ae aegypti population is still tolerant to the insecticide deltamethrin (91,25% in mortality rate). However, molecular analysis confirms the presence of a mutation at position 1016 of the voltage-gated sodium channel (VGSC) gene (homozygous mutation), which is known to be the primary target of pyrethroid insecticides. These findings indicate that although phenotypic resistance has not yet fully developed, the mosquito population has shown early indications of target-site insensitivity to deltamethrin, which has the potential to develop into resistance under sustained selection pressure. Further use of deltamethrin insecticide may potentially increase phenotypic resistance, namely a decrease in mosquito mortality after exposure to deltamethrin insecticide. Therefore, an integrated vector control strategy and routine monitoring of mosquito resistance status in the region are required.
Efektivitas Sosialisasi Protokol Kesehatan dalam Upaya Penanganan Covid-19: Studi di Desa Ambulu Kabupaten Jember Yuliani Dewi; Purwatiningsih Purwatiningsih
ABDI UNISAP: Jurnal Pengabdian Kepada Masyarakat Vol. 3 No. 2 (2025): ABDI UNISAP: Jurnal Pengabdian Kepada Masyarakat
Publisher : UPT Publikasi dan Penerbitan Universitas San Pedro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59632/abdiunisap.v3i2.496

Abstract

Sejak Maret 2020, pandemi Covid-19 berdampak besar di Indonesia, terutama di Jawa Timur dan Jember. Kegiatan ini bertujuan mengedukasi warga Desa Ambulu, Jember, tentang protokol kesehatan untuk mengurangi penyebaran Covid-19. Inisiatif ini dilakukan pada Oktober 2021 di RW 03 Krajan Ambulu. Metode pelaksanaan mencakup sosialisasi interaktif dengan ceramah, demonstrasi, dan diskusi. Materi berfokus pada situasi pandemi di Indonesia khususnya Jawa Timur, serta edukasi penerapan protokol kesehatan. Peserta sangat antusias dan aktif berdiskusi tentang Covid-19 dan protokol kesehatan. Sebagian besar peserta sudah mengenal protokol kesehatan, seperti menjaga jarak, mencuci tangan, dan memakai masker. Namun, mencuci tangan setelah dari tempat umum jarang dilakukan, dan menjaga jarak di keramaian masih tidak konsisten. Banyak peserta paham pentingnya vaksinasi untuk mencegah Covid-19. Keberhasilan edukasi ini menunjukkan pentingnya keterlibatan masyarakat dalam kesehatan. Program selanjutnya bisa fokus memperkuat kebiasaan mencuci tangan dan menjaga jarak, terutama di area yang belum konsisten. Kampanye informasi tentang keamanan dan efektivitas vaksin juga bisa membantu meningkatkan vaksinasi dan perlindungan komunitas dari Covid-19.