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All Journal THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Klinikal Sains (Jurnal Analis Kesehatan) Cendekia Journal of Pharmacy Indonesian Journal of Global Health research Jurnal Farmasetis Prosiding Seminar Nasional Pengabdian Kepada Masyarakat: Peduli Masyarakat KESMAS UWIGAMA: Jurnal Kesehatan Masyarakat
Dwi Haryatmi
Sekolah Tinggi Ilmu Kesehatan Nasional
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Social Sciences Other

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GAMBARAN KEJADIAN MALARIA DI KABUPATEN ACEH BARAT SETELAH STATUS ELIMINASI TAHUN 2018 Dwi Haryatmi; Wuryawan Wuryawan
Klinikal Sains : Jurnal Analis Kesehatan Vol. 14 No. 1 (2026): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v14i1.8252

Abstract

After achieving malaria elimination status in 2018, West Aceh Regency faced challenges in the form of imported malaria cases and the emergence of zoonotic cases due to interactions with forests. This descriptive study with a secondary data analysis design (Retrospective Cohort Study) aims to analyze temporal trends, demographic characteristics (people), regional distribution (places), and types of malaria parasites for the period 2019–2025. Total sampling was conducted on all malaria cases at the West Aceh Regency Health Office with an accumulation of 61 cases, where trend analysis was calculated using simple linear regression. The results showed that all samples were positive for malaria where there was a temporal spike in 2025 of 19 cases. Infection cases were absolutely dominated by men (98.2%), all of whom were in the productive age group (100%), worked in the military sector (80.32%), were forest encroachers, or illegal gold miners, with the majority being imported cases (96.72%). From the characteristics of the parasite, the threat of an etiological shift from human malaria (P. falciparum and P. vivax) to zoonotic malaria of long-tailed macaques, Plasmodium knowlesi, was found. In conclusion, the macro-elimination status of the region remains maintained with an Annual Parasite Incidence (API) value of <1 per 1,000 population (API = 0.087) but there is a vulnerability to post-elimination malaria in West Aceh triggered by occupational risks, high geographic mobility of productive-age men, and the threat of hidden transmission of P. knowlesi zoonosis due to the impact of forest deforestation
DNA Quality Testing of Isolated Stored Giemsa Blood Smear Samples using Geneius™ Micro gDNA Extraction Kit as An Alternative Sample for Malaria Molecular Examination Dewi Saroh; Dwi Haryatmi; Rendyansah Agung Widodo
Indonesian Journal of Global Health Research Vol 7 No 1 (2025): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v7i1.4844

Abstract

The gold standard for malaria examination is microscopic examination. However, microscopic examination has many shortcomings, namely the difficulty of distinguishing between Plasmodium sp. species when mixed infections occur. Other malaria examinations for screening can be done with RDT. However, it also has shortcomings, namely the minimum number of parasites that can be detected is 100 parasites/ul. Molecular examination is the most sensitive and accurate examination in the diagnosis of malaria. The initial stage of molecular examination is the DNA isolation stage. This DNA isolation is very important in determining the success of molecular examination. Isolation of Plasmodium sp. DNA using blood smear samples is still rarely done and there is no standard procedure for isolating DNA from blood smears. The purpose of this study was to determine whether stored Giemsa blood smears can be used as samples in DNA isolation in malaria cases. The method used in isolation is using the Geneius™ Micro gDNA Kit. The results of the study were that stored Giemsa blood smear samples of Plasmodium vivax for 3 months could be isolated using the Geneius™ Micro gDNA Kit and could be used as DNA templates for amplification with target gene primers.
Comparative Analysis of Staining Time Using Giemsa 10% on the Results of Malaria Blood Preparations M. Richo Realdy Wirawan; Dwi Haryatmi
Indonesian Journal of Global Health Research Vol 7 No 4 (2025): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v7i4.6347

Abstract

Malaria is a parasitic infectious disease that remains a significant public health problem in Indonesia, particularly in endemic areas. Rapid and accurate diagnosis of malaria is essential to prevent serious complications. The 10% Giemsa staining method is a rapid technique for microscopic examination of malaria, but the staining time standard needs to be validated for each batch of staining solution used in local laboratories. This study aims to analyze the comparison of malaria blood smear staining results using 10% Giemsa with different staining durations of 10 minutes, 20 minutes, and 30 minutes at Natar Medika Hospital. This research employed a cross-sectional approach with observational analysis of thin blood smears from 15 malaria-positive blood samples. The blood smear evaluation used a scoring system: a score of 0 was given for poor staining results (unclear background, unstained or non-blue cytoplasm, and unstained or non-red nucleus), and a score of 1 for good staining results (clear background, blue cytoplasm, and red nucleus). The results showed that staining for 20 minutes produced the best quality thin blood smears, characterized by a clear background, blue cytoplasm, and red nucleus. Statistical analysis using the Kruskal-Wallis test indicated significant differences between staining durations (p < 0.05). Therefore, staining for 20 minutes using 10% Giemsa is recommended to obtain optimal microscopic results in malaria diagnosis at Natar Medika Hospital.
Sensitivity and Specificity of Malaria Rapid Diagnostic Test (RDT) Siti Retno Yuniatun; Dwi Haryatmi
Indonesian Journal of Global Health Research Vol 7 No 4 (2025): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v7i4.6362

Abstract

Malaria remains a significant public health issue in many regions, and early diagnosis is crucial for effective treatment. Rapid Diagnostic Tests (RDT) are commonly used for malaria detection due to their speed and ease of use, but their diagnostic accuracy, particularly in regions with varying levels of parasitemia, remains a subject of ongoing research. This study aimed to evaluate the performance of Rapid Diagnostic Tests (RDT) for malaria diagnosis at Puskesmas Hanura, Teluk Pandan District, Pesawaran Regency, by assessing the sensitivity and specificity of the test. Methods: This analytical observational study employed a cross-sectional approach, analyzing blood samples from malaria suspect patients collected between December 2024 and February 2025, with a total sample size of 50 samples. The diagnostic performance of RDT was compared to the gold standard of microscopy using thick and thin blood smears. Sensitivity and specificity were calculated based on the results of both methods. The sensitivity of the RDT was found to be 93.5%, indicating that the test was highly effective in detecting true positive malaria cases, particularly in individuals with high parasitemia. The specificity was 100%, demonstrating that the RDT accurately identified malaria-free individuals with no false positive results. However, the study also identified two false negative cases, suggesting that the RDT's sensitivity could be reduced in cases of low parasitemia. The Rapid Diagnostic Test demonstrated excellent sensitivity and specificity in detecting malaria at Puskesmas Hanura, with no false positive results and a high rate of true positive detection. However, the test showed some limitations in detecting low parasitemia, emphasizing the need for confirmatory diagnostic techniques, such as microscopy or molecular methods, especially in areas with low transmission or mild infections.
Co-Authors A.Dicha Putri Maharani Adhi Kumoro Setya Andya Wulansari Dewi Saroh Fitria Diniah Janah Sayekti Fitria Diniah Jannah Sayekti Indah Tri Susilowati livana ph M. Richo Realdy Wirawan Muhammad Taufiq Qurrahman Muhammad Taufiq Qurrohman Rendyansah Agung Widodo Siti Retno Yuniatun Tri Harningsih Wuryawan Wuryawan Yoannda R Sharone