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All Journal Jurnal Akuakultur Rawa Indonesia JURNAL ILMIAH PLATAX ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Jurnal Sumberdaya Akuatik Indopasifik Omni-Akuatika Jurnal Ilmu Kelautan Kepulauan Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) JURNAL PERIKANAN TROPIS Jurnal Abdi Insani Jurnal Pengabdian kepada Masyarakat Nusantara Juvenil: Jurnal Ilmiah Kelautan dan Perikanan Journal of Fisheries & Marine
Muhammad Aris
Program Studi Budidaya Perairan. Universitas Khairun, Ternate
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Social Sciences

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KAJIAN FILOGENETIK IKAN TUNA (Thunnus spp) SEBAGAI DATA PENGELOLAAN DI PERAIRAN SEKITAR KEPULAUAN MALUKU, INDONESIA Nebuchadnezzar Akbar; Muhammad Aris; Muhammad Irfan; Irmalita Tahir; Abdurrachman Baksir
Jurnal Kelautan Vol 11, No 2 (2018)
Publisher : Department of Marine Sciences, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.679 KB) | DOI: 10.21107/jk.v11i2.3459

Abstract

Ikan tuna (Thunnus Spp) adalah ikan pelagis yang memilili kemampuan migrasi dan nilai komersial. Kondisi oseanografie Maluku Utara dan Ambon mendukung kelimpahan stok populasi sumberdaya. Pengetahuan filogenetik dapat membantu menunjukan hubungan evolusioner dari suatu organisme yang disimpulkan dari data morfologi dan molekuler. Tujuan penelitian untuk mengetahui filogenetik ikan tuna di perairan Maluku Utara dan Ambon. Penelitian ini menggunakan metode PCR-Sequencing. Analisis molekuler menggunakan Polymerase Chain Reaction (PCR) dengan primer CRK-CRE, elektroforesis. Rekonstruksi pohon filogenetik menggunakan metode Neighbor joining dan model evolusi Kimura 2-parameter dilakukan dengan aplikasi MEGA5. Hasil penelitian filogenetik menemukan terdapat empat empat clade spesies ikan tuna yang saling berbeda (tuna mata besar ; tuna sirip kuing ; tuna alalunga ; cakalang ). Jarak genetik tuna mata besar (T.obesus)  dengan tuna sirip kuning (T.albacares)  adalah 0.09, tuna mata besar (T.obesus)  dengan tuna alalunga (T.albacore) adalah 0.19, tuna sirip kuning (T.albacares)  dengan tuna alalunga (T.albacore),  sebesar 0.21,  tuna mata besar (T.obesus)  dengan tuna alalunga (T.albacore)  cakalang (K.pelamis) adalah 0.34, cakalang (K.pelamis) dengan tuna alalunga (T.albacore) adalah  0.39 dan tuna sirip kuning (T.albacares)  dengan Cakalang (K.pelamis) adalah 0.34. Semua hasil menunjukan perbedaan genetik yang signifikan, genetik spesies tuna berasal dari satu kelompok dan filogeografi memiliki batas distribusi yang nyata antar satu dengan yang lain.Kata Kunci :     Thunnus, Polymerase Chain Reaction (PCR), Pohon Filogenetik, primer CRK-CRE, , Neighbor joining, Kimura 2-parameter, jarak genetik, MEGA5, filogeografi.  ABSTRACTTuna (Thunnus Spp) is a pelagic fish that has migration capabilities and commercial value. The condition of North Maluku and Ambon oceanographies supports the abundance of resource population stocks. Phylogenetic knowledge can help show the evolutionary relationship of an organism inferred from morphological and molecular data. The aim of the study was to determine phylogenetic of tuna in the waters of North Maluku and Ambon. This study used the PCR-Sequencing method. Molecular analysis uses a polymerase chain reaction (PCR) with CRK-CRE primer, electrophoresis. Reconstruction of the phylogenetic tree using the Neighbor joining method and the Kimura 2-parameter evolution model was carried out with the MEGA5 application. The results of phylogenetic research found that there were four four different clades of tuna species (bigeye tuna; kuing fin tuna; alalunga tuna; cakalang). The genetic distance of big eye tuna (T.obesus) with yellow fin tuna (T.albacares) is 0.09, bigeye tuna (T.obesus) with tuna alalunga (T.albacore) is 0.19, yellow fin tuna (T.albacares) with tuna alalunga (T.albacore), for 0.21, big eye tuna (T.obesus) with alalunga tuna (T.albacore) cakalang (K.pelamis) is 0.34, cakalang (K.pelamis) with alalunga tuna (T.albacore) is 0.39 and yellow fin tuna (T.albacares) with Cakalang (K. pelamis) are 0.34. All results show significant genetic differences, genetic tuna species come from one group and filogeography has a real distribution boundary between one another.Keywords: Thunnus, Polymerase Chain Reaction (PCR), Phylogenetic Tree, CRK-CRE primer, Neighbor joining, Kimura 2-parameter, genetic distance, MEGA5, filogeography.
BAKTERI PATOGEN PADA KEPITING KELAPA (Birgus latro) Muhammad Aris; Sudirto Malan
JURNAL PERIKANAN TROPIS Vol 8, No 1 (2021)
Publisher : Universitas Teuku Umar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35308/jpt.v8i1.2555

Abstract

Coconut crab (Birgus latro) is a fishery resource with high economic value. Coconut Crab is only found in eastern Indonesia, covering Sulawesi, Maluku and North Maluku. One of the habitat of Coconut Crab in North Maluku is Moor Island, Central Halmahera. The coastal typology of this island is predominantly rocky with many gaps and small caves, as well as several steep slopes with dominant vegetation conditions of coconut, beach pandanus and other coastal plants. Walnut Crab is often used as a consumption ingredient because it has delicious taste and high nutritions. However, the use of crustaceans for consumption can also be dangerous. This is because crustaceans also contain several pathogenic bacteria. This study aimed to identify pathogenic bacteria isolated from Coconut Crab which is the largest crustacean in the world. 5 samples of Coconut Crab were taken from Moor Island. Isolated samples marked KK1, KK2, KK3, KK4 and KK5. Pure bacterial isolates were obtained from Coconut Crab samples, evaluated for colony type and identified based on biochemical characterization. The types of pathogenic bacteria identified in samples KK1, KK2, KK3, KK4 and KK5 were Escherichia coli, Pseudomonas sp. and Staphylococcus sp.
Population Genetics and Phylogeography of Bigeye Tuna in Moluccas Seas, Indonesia Nebuchadnezzar Akbar; M. Irfan; Muhammad Aris
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 23, No 4 (2018): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (515.071 KB) | DOI: 10.14710/ik.ijms.23.4.145-155

Abstract

The bigeye tuna (Thunnus obesus) is a migratory fish which can be found in the Atlantic, Indian and Pacific oceans. This fish has a commercial value and has been exploited worldwide including in Indonesia. The exploitation might affect the genetic diversity and population structure. The fact that the population stock resource is abundant and following fishing activities are increasing, study on population genetic and phylogeography canbe used as information to determine the status of the fish population based on genetic data. The study was conducted to investigate population genetic, and phylogeography of bigeye tuna in the North Moluccas and South Mollucas Seas, Indonesia. A total of 60 tissue bigeye tuna samples were collected from two study sites. The samples were amplified using mitochondrial DNA control region. Within population genetic diversity was revealed of 0.985 and 1.00 in North Moluccas and in south Moluccas, respectively, while between populations was 0.989. The genetic distance within population of North Moluccas (0.029) and South Mollucas (0.24) was very low, and all population was 0.027. The genetic distance between population of North Mollucas and South Mollucas was 0.025, South Mollucas and all population was 0.023, and all population with Norht Mollucas was 0.027. The genetic distance of North Mollucas and Pacific Ocean was 0.029, South Mollucas and Pacific Ocean was 0.023, North Mollucas, South Mollucas and Indian Ocean was 0.32. The Fst value between populations (0.990) showed that the two populations were not genetically different. A similar result showed from the phylogenetic trees analysis which individual of bigeye tuna was randomly clustred between North Moluccas and South Mollucas population, indicating that they were genetically close and from the same population. The population bigeye tuna from the North Mollucas and the South Mollucas exhibits no apparent phylogeographic distribution.
Phylogenetic of tuna fish (Thunnus spp.) in North Mollucas Sea, Indonesia Nebuchadnezzar Akbar; Muhammad Aris; Muhammad Irfan; Abdurrachman Baksir; Surahman Surahman; Hawis H. Madduppa; Raismin Kotta
Jurnal Iktiologi Indonesia Vol 18 No 1 (2018): February 2018
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v18i1.370

Abstract

The tuna fish (Thunnus spp.) is highly migratory and commercial tuna fishery. The fish tuna abudance supported ocea-nography and geography condition in North Mallucas Sea. The fishery targets catch increase on fish tuna provided a view of the need for assessment of phylogenetic tuna. The study was conducted to infer the phylogenetic in North Mollucas Sea. The research method was PCR-Sequensing. Moleculer analysis included extraction, Polymerase Chain Reaction (PCR), electrophoresis and DNA sequencing in control region mtDNA locus. Phylogenetic reconstructed with Neigbor joining with Kimura 2-parameter model using MEGA5. The result showed that four clade (bigeye, yellowfin, alalunga and skipjack). Genetic distance between bigeye with yellowfin was (0.084), bigeye with alalunga (0.163), ye-llowfin with alalunga (0.174), bigeye with skipjack (0.294), skipjack with alalunga (0.312) and yellowfin with skipjack (0.297). The overall result showed significant genetic different. That information explain about one populations species tuna. The tuna phylogeography unlimitedin geographic distributions. Abstrak Ikan tuna (Thunnus spp.) adalah ikan pelagis yang memiliki kemampuan ruaya dan nilai komersial. Kondisi oseanogra-fis dan letak geografis mendukung kelimpahan stok sumber daya ikan tuna di Perairan Maluku Utara. Aktifitas penang-kapan yang meningkat memberikan pandangan perlu adanya pengkajian filogenetik ikan tuna. Penelitian ini bertujuan untuk memperoleh informasi filogenetik ikan tuna di perairan Maluku Utara. Metode yang digunakan adalah metode PCR-Sekuensing pada lokus mtDNA control region. Analisis molekuler meliputi ekstraksi, Polymerase Chain Reaction (PCR), elektroforesis dan sekuensing DNA. Rekonstruksi pohon filogenetik dengan metode Neighbor joining dengan model evolusi Kimura 2-parameter dilakukan menggunakan aplikasi MEGA5. Hasil penelitian menemukan empat clade spesies ikan tuna yang berbeda (tuna mata besar, sirip kuning, alalunga, dan cakalang). Jarak genetik tuna mata besar (Thunnus obesus) dengan sirip kuning (Thunnus albacares) adalah 0,084; tuna mata besar dengan tuna alalunga (Thunnus albacore) adalah 0,163; tuna sirip kuning dengan tuna alalunga sebesar 0,174; tuna mata besar dengan caka-lang (Katsuwonus pelamis) adalah 0,294; cakalang dengan tuna alalunga adalah 0,312; dan tuna sirip kuning dengan cakalang adalah 0,297. Semua hasil menunjukkan perbedaan genetik signifikan. Namun dapat dijelaskan bahwa spesies tuna berasal dari satu keturunan. Filogeografi tuna tidak memiliki batas distribusi yang nyata spesies.
Pemanfaatan Bahan Lokal Biji Pala (Myristica sp.) Sebagai Bahan Anestesi Pada Kegiatan Budidaya Ikan Hias Blue Devil (Chrysiptera cyanea) Muhammad Aris; Fatma Muchdar
Jurnal Sumberdaya Akuatik Indopasifik Vol 5 No 2 (2021): Mei
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46252/jsai-fpik-unipa.2021.Vol.5.No.2.109

Abstract

Blue Devil (Chrysiptera cyanea) is one of the very popular seawater ornamental fish commodities that is often sought after in international trade because of its attractive morphology. The trading ornamental fish commodities are very different from the consumption fish commodity because the trade is alive. Handling and transportation play a critical role in this system. However, the problem of delivering these goods is high mortality happened before the fish arrive at their destination. In this study, the delivery of ornamental fish using anesthetic ingredients. The anesthetic ingredients are MS-222, benzocaine, 2-phenoxyethanol, and quinaldine sulfate. The application of this material is not recommended because it will harm fish that can reduce fish immunity. The application of natural anesthetic ingredients can be used as an alternative environment. Nutmeg (Myristica sp.) is one of the local natural ingredients that have anesthetic potential. Nutmeg (Myristica sp.) contains essential oils that are useful as adaptive substances. This study aims to determine the potential of local Nutmeg (Myristica sp.) Seeds as an anesthetic material for Blue Devil (Chrysiptera cyanea) by lethal concentration / LC-50 testing. LC-50 testing in this study is an initial step to determine the appropriate anesthetic dose for Blue Devil (Chrysiptera cyanea). Making extracts from nutmeg seeds (Myristica sp.) Aims to obtain essential oils. The procedure for obtaining nutmeg (Myristica sp.) Essential oils are carried out by steam distillation. The results showed that the seeds of the Nutmeg plant (Myristica sp.) Were very influential for Blue Devil (Chrysiptera cyanea) with a very significant dose of 10ml, but not recommended for use. For development as an anesthetic material, then use a dose <2 ml.
Genetic Population Structure of Yellowfin Tuna (Thunnus albacares) as Based Data of Fish Conservation in North Mallucas Sea Nebuchadnezzar Akbar; Muhammad Aris
Journal Omni-Akuatika Vol 14, No 3 (2018): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (532.686 KB) | DOI: 10.20884/1.oa.2018.14.3.457

Abstract

Tuna is a migratory species with high economic value. Utilization of tuna is increasing and growing every year. Fishing intensity of tuna in the Maluku Sea is high and can disrupt the population. The existence of the population will be threatened if not managed properly. The latest genetic information approach is necessary to prevent the population decline. The purpose of this research was to know the genetic structure of yellowfin tuna in North Maluku waters, Indonesi. Sampling was conducted in May-July 2016 in Morotai Island, Obi and Sanana, while secondary data was gathered in Ternate Island, Bacan and Ambon. A total of 72 samples were collected and analyzed. The result of the study found that the base length (bp) of the control region of mtDNA was found to be 512 bp (base pairs). the genetic distance in the nearest population is at Sanana and Obi (0.025). The results of genetic distance analysis between population found genetic similarity between Morotai-Sanana (0,021), Obi-Sanana (0,025), Obi-Morotai (0,026) and Ambon-Sanana (0,026), while the furthest genetic distance was found in Ternate-Bacan (0,040) and Ternate-Obi (0,042). The pairwise comparison test (Fst) shows a few genetic differentiation between yellowfin tuna populations. The value (Fst) of the yellowfin tuna population shows a strong gene flow between populations. The haplotype distribution shows a relationship between haplotypes in both yellowfin tuna, thus failing to show clade between different geographic locations. Unsustainable use can harm the population through genetic quality. Several approaches should be taken to support the life cycle of yellowfin tuna. The overall result shows that there has not been any change of genetic structure of yellowfin tuna in North Maluku Sea. Keywords : Haplotype, genetic distance, North Maluku, yellowfin tuna, pairwise comparison test
Growth and survival of nener Bandeng (Chanos chanos) with different stocking densities Muhammad Aris; Aras Syazili; Ardi Buton
Jurnal Ilmu Kelautan Kepulauan Vol 4, No 1 (2021): Jurnal Ilmu Kelautan Kepulauan
Publisher : Fakultas Perikanan dan Kelautan. Universitas Khairun

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33387/jikk.v4i1.3356

Abstract

SOSIALISASI DAN PELATIHAN TEKNOLOGI BUDIDAYA IKAN NILA SISTEM BIOFLOK PADA KELOMPOK USAHA BERSAMA Waode Munaeni; Muhammad Aris; Ismi Musdalifah Darsan; Rusmawati Labenua; Disnawati Disnawati
Jurnal Abdi Insani Vol 9 No 4 (2022): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v9i4.797

Abstract

Intensive cultivation of tilapia at high densities causes high waste, which can result in low water quality. This will also make it easier for fish to get sick. Another problem in tilapia cultivation is the high use of feed. These problems cause low productivity in tilapia aquaculture. One solution is to cultivate tilapia using the biofloc system. The biofloc system has been shown to increase fish growth, improve water quality, and the floc that forms can be used as a feed source, reducing the need for artificial feed. The purpose of this community service activity is to provide insight regarding tilapia farming using biofloc technology and training on how to make biofloc for tilapia cultivators in fish farming groups in Lake Ngade. Activities are carried out at Fitu Village, near Lake Ngade, from June to August 2022. Activities include: 1) socialization with the aim of broadening tilapia cultivators' insights using biofloc technology; 2) hands-on training on how to make floc in buckets, flock measurement, stocking tilapia, rearing, and harvesting. The results of the socialization activities were able to pique the participants' interest and increase their knowledge, particularly those of the tilapia cultivators in Lake Ngade, about the biofloc material provided. It can be seen from the interaction between participants and resource persons regarding the material provided during socialization activities. During the training activities, cultivators follow all stages, starting from how to make flocks, measuring flocks, stocking tilapia, rearing them, and harvesting them. During rearing, the practices given are 1) feed management; 2) flock volume measurement and flock management; and 3) measurement of several water quality parameters such as pH, temperature, hardness, nitrite, and nitrate. The conclusion from this community service activity is that the socialization and training carried out can increase the participants' insights and skills, especially for tilapia cultivators using biofloc technology, starting from the method of making flocks, measuring flocks, stocking tilapia, maintenance, including feeding and measuring quality water, and harvest.
Pelatihan Budidaya Rumput Laut Sistem Jaring Kantong di Desa Tuada Kabupaten Halmahera Barat Muhammad Aris; Tamrin; Sudirto Malan; Agustinus Herlis Maholle; Waode Munaeni
Jurnal Pengabdian kepada Masyarakat Nusantara Vol. 3 No. 2.1 Desember (2022): SPECIAL ISSUE
Publisher : Cv. Utility Project Solution

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (765.918 KB)

Abstract

Rumput laut Kappaphycus alvarezii merupakan komoditas akuakultur yang memiliki nilai ekonomis tinggi. Pengembangan budidaya rumput laut di Maluku Utara saat ini mulai berkembang. Pembudidaya rumput laut yang terdapat di Kabupaten Halmahera Barat, Provinsi Maluku Utara didorong menjadi kampung budidaya rumput laut oleh Kementerian Kelautan dan Perikanan (KKP). Kegiatan ini bertujuan untuk memberikan pelatihan dan pendampingan manajemen budidaya rumput laut sistem teknologi jaring kantong kepada pembudidaya. Kegiatan ini dilakukan mulai bulan September sampai November 2022, bertempat di Desa Tuada, Kabupaten Halmahera Barat, Maluku Utara. Kegiatan ini dimulai dari sosialisi inovasi kemudian dilanjutkan dengan pelatihan secara langsung kepada pembudidaya, mulai dari persiapan hingga pemeliharaan. Secara rinci, kegiatan yang dilakukan meliputi : 1) sosialisasi inovasi; 2) persiapan alat dan bahan; 3) pembuatan jaring kantong; 4) pemasangan tali; 5) penyediaan bibit rumput laut; 6) pemasukan bibit dalam kantong; 7) penempatan di lokasi budididaya dan 8) pemeliharaan. Dengan dilakukannya kegiatan ini, diharapkan pembudidaya memiliki wawasan dan keterampilan untuk meningkatkan produksi rumput laut di Kabupaten Halmahera Barat.
Health Condition of Tridacna sp. in the waters of Obi Island, Indonesia Tamrin Tamrin; Muhammad Aris
Jurnal Ilmiah PLATAX Vol. 8 No. 2 (2020): ISSUE JULY-DECEMBER 2020
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.8.2.2020.30672

Abstract

Tridacna sp. is one of the protected heritage in Indonesia, because its population has declined dramatically. Tridacna sp. is the largest type of shellfish in water. Like a clam, Tridacna sp. can be used as a bioindicator of the aquatic environment because it can accumulate more heavy metals than other aquatic organisms due to its nature as a filter feeder. This study aims to determine the health condition of Tridacna sp. in the waters of Obi Island with a histopathological analysis approach. The histopathological analysis aims to see the level of tissue damage due to the accumulation of heavy metals. Observation of water quality was also observed in this study. Water quality parameters observed in-situ are temperature, brightness, salinity, pH, and dissolved oxygen. While the water quality parameters observed ex-situ are nitrate, orthophosphate, ammonia, iron (Fe), and nickel (Ni). Observations show that the water temperature is at 27.99 ℃. Water brightness is at 13 m. Salinity is at 32.13 mg / l. The acidity of the waters is 8.64. Ammonia is at 0.4 mg / L. Nitrate is at 0.009 mg / L. Orthopedics are at 0.016 mg / L. Dissolved oxygen waters are at 3.77 mg / L. The iron (Fe) level of water is at 0.6 mg / L. The level of nickel (Ni) waters is at 0.06 mg / L. This study shows the health condition of Tridacna sp. the histological approach shows that the condition of the network has changed. Symptoms of this change indicate the condition of the Tridacna sp. degeneration and cell necrosis. This change is thought to be influenced by heavy metals. Heavy metal content in liquids exceeds the quality standard threshold.Keywords:  Tridacna sp.; Heavy metal; Water quality; histopathological.
Co-Authors . Tamrin Abdurrachman Baksir Abdurrachman Baksir Agustinus Herlis Maholle Aras Syazili Ardi Buton Disnawati Disnawati Hawis H Madduppa Irmalita Tahir Ismi Musdalifah Darsan M. Irfan Masrida Ode Ane Muchdar, Fatma Nebuchadnezzar Akbar Raismin Kotta Rusmawati Labenua Rusmawati Labenua Samadan, Gamal M Sudirto Malan Sudirto Malan Surahman Surahman Taufiq Abdullah WAODE MUNAENI