Garuda - Garba Rujukan Digital

Article Per Year (5 Year)

p-Index From 2020 - 2025

P-Index

This Author published in this journals

All Journal Medical Journal of Indonesia Pharmaceutical Sciences and Research (PSR)

HANS-JOACHIM FREISLEBEN

Medical Research Unit, Faculty of Medicine, University of Indonesia

Author-ID : 267773

Medicine & Pharmacology

Published : 16 Documents Claim Missing Document

Claim Missing Document

Articles

Title

1 2

20 years German-Indonesian Medical Association (GIMA) / Deutsch-Indonesische Gesellschaft für Medizin (DIGM e.V.) Bundschu, Hans-Dieter; Freisleben, Hans-Joachim
Medical Journal of Indonesia Vol 25, No 2 (2016): June
Publisher : Faculty of Medicine Universitas Indonesia

[no abstract available]

International collaboration for implementation of equal access to family-based primary healthcare in Indonesia Haier, Jörg; Schäfers, Jürgen; Freisleben, Hans-Joachim
Medical Journal of Indonesia Vol 26, No 2 (2017): June
Publisher : Faculty of Medicine Universitas Indonesia

[No abstract available]

Reconsidering markers of oxidative stress Freisleben, Hans-Joachim
Medical Journal of Indonesia Vol 22, No 4 (2013): November
Publisher : Faculty of Medicine Universitas Indonesia

[no abstract available]

The preventive effect of Mangifera foetida L. leaf extract administered simultaneously to excess iron on markers of iron overload in Spraque-Dawley rats Fajri, Purnama; Estuningtyas, Ari; Louisa, Melva; Freisleben, Hans-Joachim
Medical Journal of Indonesia Vol 26, No 4 (2017): December
Publisher : Faculty of Medicine Universitas Indonesia

Background: Recently, there is no agent available for the prevention of iron overload (IO) in thalassemia patients. Previous studies showed that Mangifera foetida L. leaf extracts reduced the levels of iron in IO in vitro and in vivo models. The present study aimed to determine the efficacy of Mangifera foetida L. leaf extract in the prevention of IO induced in rats.Methods: Thirty male Sprague-Dawley rats were divided into 5 groups: control (untreated), IO, 3 treatment groups with leaf extract equivalent to 50, 100, and 200 mg of mangiferin per kg BW. Fe-dextran (15 mg) was administered intraperitoneally twice a week for 4 weeks to all groups except control (IO, DSM50, DSM100, and DSM200). Urine and blood samples were taken before and after treatments. After 4 weeks of treatment, rats were terminated, and samples of spleen, liver, and heart were taken. Ferritin and mangiferin levels and SOD activities were determined in plasma. Iron levels were measured in plasma, urine, and spleen.Results: Experimental IO increased plasma Fe content 4.23 times and plasma ferritin levels 6.9 times vs normal. Mangifera foetida L. leaf extract DSM50 resulted in the highest blood levels of 212 ng mangiferin per mL and moderately, although not significant, prevented increased plasma ferritin levels and IO in organs and protected against oxidative stress.Conclusion: Aqueous Mangifera foetida L. leaf extract may be useful to prevent IO and oxidative stress in thalassemia patients.

Stable archaeal tetraether lipid liposomes for photodynamic application: transfer of carboxyfluorescein to cultured T84 tumor cells Oertl, Anton; Antonopoulos, Emmanouil; Freisleben, Seruni U.; Freisleben, Hans-Joachim
Medical Journal of Indonesia Vol 25, No 4 (2016): December
Publisher : Faculty of Medicine Universitas Indonesia

Background: Archaeal membranes have phytanyl ether lipids instead of common fatty acid-glycerol esters in bacterial and eukaryotic cells. Sulfolobus and Thermoplasma species have unique membrane-spanning tetraether lipids (TEL), which form stable liposomes. Recently, we cultured Thermoplasma species from the Indonesian volcano Tangkuban Perahu and isolated TEL. The purpose of this in vitro study is to investigate the transfer of fluorescent dye from stable TEL liposomes to cultured colon carcinoma cells.Methods: TEL was extracted from cultured cells with chloroform-methanol (1:1), then it was fractionated and purified via diethylaminoethyl-cellulose-acetate columns and activated charcoal for the formation of stable liposomes. For the fluorescence exchange assay, TEL liposomes were loaded with water-soluble carboxyfluorescein (CF). Staining experiments were conducted with various cell cultures, and T84 colon carcinoma cells were chosen for the main experiments. Liposome stability was tested by light scattering and electron microscopic size determinations as well as by unspecific CF release at low pH (6.0–7.4) and increased temperature (4–50°C/70°C).Results: TEL liposomes exhibit high stability and extremely low proton permeability at low pH. CF staining of cultured T84 colon carcinoma cells appeares more intensive from TEL liposomes than from dipalmitoylphosphatidylcholine liposomes.Conclusion: The results of this in vitro study demonstrate CF staining of colon carcinoma cells and high stability of TEL liposomes at low pH, matching the condition in the gastro-intestinal (GI) route and in the urogentital (UG) tract. For this reason, in vivo studies on liposomal fluorescent photosensitizers for topical application of photodynamic cancer therapy in the GI and UG tracts should be carried out.

Co-Authors A.A. Ketut Agung Cahyawan W Alida Harahap Ani R. Prijanti Antonopoulos, Emmanouil Ari Estuningtyas, Ari Bethy S. Hernowo Budi Kurniawan Bundschu, Hans-Dieter David Tandian Fajri, Purnama Frans Ferdinal Freisleben, Seruni U. Haier, Jörg Isnani A.S. Suryono Jenny Hidayat Krista Kuebel-Thiel Melva Louisa Mohamad Sadikin Mohammad A. Aulia Na P. Bo Nia Yuliatri Oertl, Anton Pustika A. Wahidiyat Rahmawati Ridwan Renindra A. Aman Rondang R. Soegianto Rostika Flora Schäfers, Jürgen Septelia I. Wanandi Seruni K.U. Freisleben Soehardjo Poertadji Sri Handayani Syaiful Ichwan Tiwuk Susantiningsih

Title Search

Found 15 Documents Search Journal : Medical Journal of Indonesia

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 15 Documents
Search
Journal : Medical Journal of Indonesia