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All Journal Buletin Peternakan Jurnal Ilmu Ternak Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) JURNAL ILMIAH PETERNAKAN TERPADU Buletin Peternakan Tropis (Bulletin of Tropical Animal Science) Jurnal Kedokteran Hewan
Sigit Bintara
Faculty Of Animal Science, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Journal : Buletin Peternakan

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Pengaruh Pencucian Sperma dengan Lama Waktu Sentrifugasi yang Berbeda Terhadap Kualitas Sperma Kambing Bligon (Effect of Sperm Washing with Different Centrifugation Duration on Sperm Quality of Bligon Buck) Sigit Bintara
Buletin Peternakan Vol 34, No 2 (2010): Buletin Peternakan Vol. 34 (2) Juni 2010
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v34i2.91

Abstract

The objective of the experiment was to examine the effect of sperm washing using SpermRinse with different centrifugation duration (15, 20, and 25 minutes) on the quality of Bligon spermatozoa. Sperm was collected usingartificial vagina from six bucks of 2 (two) year olds. Data were collected for motility, viability, and abnormality of the spermatozoa. Characteristics of the fresh sperm were analyzed based on mean and deviation standard, and followed by Duncan’s New Multiple Range Test (DMRT). The results showed that washing Bligon buck sperm using SpermRinse with different centrifugation times had very significant effect (P≤0.01) on the motility, viability, and abnormality ofspermatozoa. It is concluded that washing Bligon buck sperm using SpermRinse with centrifugation at 1.500 rpm (r = 5.5 cm) could increased sperm quality, of which 20 minutes was the best centrifugation time.(Key word: Bligon Buck, Sperm, SpermRinse, Sperm quality)
Stimulasi Laser sebagai Alternatif untuk Induksi Estrus pada Kambing Bligon (Laser Stimulation as an Alternative for Estrus Induction on Bligon Goats) Sigit Bintara
Buletin Peternakan Vol 34, No 1 (2010): Buletin Peternakan Vol. 34 (1) Februari 2010
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v34i1.102

Abstract

Reproduction is a very important factor in maintaining livestock productivity. For better reproductivity, estrus induction is one of very important ways to be performed. Estrus induction is usually performed with hormonal method,such as application of PGF2α, but the disadvantage is that it is relatively expensive. For this reason, it is necessary to promote technological breakthrough to lower cost of estrus induction. The aim of this study was to know whether laserstimulation on the points of reproductive acupuncture could be performed for estrus induction on Bligon goats, as it is usually performed with hormonal method. Fourteen female Bligon goats aged 2 years old were randomly divided into two groups, each group consisted of 7 goats. Group I was stimulated with laser and group II was injected with 1.25 mg per head of PGF2α. Laser stimulation was conducted for 10 seconds on each point of acupuncture on day 1, 2, and 3, and was repeated on day 11, 12, and 13. PGF2α injection was performed on day 1 and was repeated on day 11. The observed variables were the percentage of estrus, onset of estrus, estrus duration and progesterone level when estrus occurred after the second estrus induction. The results of the study showed that laser stimulation for estrus induction did not indicate significant difference compared to PGF2α injection, in terms of the percentage of goats undergoingestrus, onset of estrus and the estrus duration. It was concluded that laser stimulation could be used for estrus induction on Bligon goats.(Key words: Laser, Estrus induction, Bligon goats)
PRE WEANING GROWTH OF BALI CALVES AT BALAI PEMBIBITAN TERNAK UNGGUL SAPI BALI Luis Tavares; Endang Baliarti; Sigit Bintara
Buletin Peternakan Vol 36, No 3 (2012): Buletin Peternakan Vol. 36 (3) Oktober 2012
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v36i3.1629

Abstract

Balai Pembibitan Ternak Unggul is a breeding center established by the Government of Indonesia (GOI) to perform preservation, breeding, breeding stock production and development, and also the distribution of breeding stock of Excellent Bali cattle at national level. In the production of excellent breeding stock through the performance test and progeny test. Pre-weaning growth is one of the important indicators for the population growth. The study was aimed to identify the pre-weaning calf growth rate of Bali cattle in 2010-2011, born from performance-test-passing-dams that were collectively reared with progeny-test-passing sires. Materials used were 84 pre-weaning Bali calves. This was a survey method-based descriptive analysis research. The results showed that in terms of the performance of pre-weaning growth, the birth weights of male and female calves were 18.37±1.65 kg and 18.27±1.29 kg, respectively. The weaning weights of male and female calves were 93.53±21.00 kg and 87.66±12.04 kg, respectively, with weaning time of 205 days. The average of daily weight gain (ADG) were 0.37±0.10 kg/head/day (male) and 0,34±0,06 kg/head/day (female). It was concluded that the pre-weaning calf growth rate of Bali cattle at the BPTU Bali in 2010-2011 were moderately high since they were born from the dams and the sires passing the performance test and progeny test.(Keywords: Pre-weaning growth, Bali cattle)
PENGARUH DOSIS SPERMA YANG DIENCERKAN DENGAN NaCl FISIOLOGIS TERHADAP FERTILITAS TELUR PADA INSEMINASI BUATAN AYAM KAMPUNG Widya Asmarawati; Kustono (Kustono); Diah Tri Widayati; Sigit Bintara; Ismaya (Ismaya)
Buletin Peternakan Vol 37, No 1 (2013): Buletin Peternakan Vol. 37 (1) Februari 2013
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v37i1.1952

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh dosis sperma untuk Inseminasi Buatan (IB) pada ayam kampung terhadap fertilitas telurnya. Dosis IB ini berguna untuk mengetahui perbandingan jantan dan betina yang paling efisien dalam suatu pemeliharaan. Sperma ditampung dan dicampur dari 5 ekor ayam Pelung jantan yang berumur sekitar 52 minggu. Sperma yang telah diketahui konsentrasinya kemudian dibagi menjadi 3 bagian dan masing-masing diencerkan dengan NaCl fisiologis sehingga diperoleh dosis akhir yaitu 25x106/0,1 ml (dosis 1); 75x106/0,1 ml (dosis 2); dan 150x106/0,1 ml (dosis 3). Sembilan ekor ayam kampung betina diinseminasi untuk mengetahui pengaruh dosis terhadap fertilitas telur. Ayam betina yang digunakan adalah ayam kampung yang berumur sekitar 30 minggu. Hasil penelitian menunjukkan bahwa perlakuan dosis sperma tidak berpengaruh pada fertilitas telur ayam kampung. Fertilitas telur untuk masing-masing perlakuan dosis 1, 2, dan 3 adalah 44,13±20,84; 66,67±23,57; dan 58,75±14,36%. Kesimpulan dari penelitian ini adalah dosis sperma tidak berpengaruh pada fertilitas telur ayam kampung.(Kata kunci: Ayam kampung, Dosis sperma, Inseminasi buatan)
PENGARUH ARAS KUNING TELUR ITIK ALABIO (Anas platyrhynchos) DALAM PENGENCER TRIS FRUKTOSA TERHADAP MOTILITAS, VIABILITAS, DAN ABNORMALITAS SPERMA KAMBING BLIGON SEBELUM DAN SESUDAH KRIOPRESERVASI Prajaningrum Luluk Miftahul Umami; Sigit Bintara; Ismaya (Ismaya)
Buletin Peternakan Vol 39, No 3 (2015): BULETIN PETERNAKAN VOL. 39 (3) OKTOBER 2015
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v39i3.7978

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh aras kuning telur itik Alabio (Anas platyrhynchos) yang diencerkan dengan tris fruktosa terhadap persentase motilitas, viabilitas, dan abnormalitas spermatozoa kambing Bligon setelah pengenceran, ekuilibrasi, prefreezing, dan post-thawing. Kualitas sperma diamati setelah pengenceran, setelah ekuilibrasi, preefreezing, dan post-thawing. Sperma seekor kambing Bligon ditampung dengan metode vagina buatan dua kali seminggu. Sperma diencerkan dengan pengencer tris fruktosa dengan aras kuning telur itik 10%, 20%, dan 30%. Data kualitas spermasegar dianalisis dengan mean dan standar deviasi, sedangkan motilitas, viabilitas, dan abnormalitas spermatozoa setelah pengenceran, ekuilibrasi, prefeezing, dan post-thawing dianalisis variansi pola searah. Hasil analisis menunjukkan bahwa penggunaan kuning telur itik dengan aras 10% memberikanmotilitas dan viabilitas sperma setelah pengenceran, setelah ekuilibrasi, setelah prefreezing , serta setelah post-thawing yang lebih baik jika dibandingkan dengan penggunaan kuning telur itik dengan aras 20% maupun 30% (P<0,05). Selain itu, abnormalitas sperma yang mendapatkan kuning telur itik dengan aras 10% juga lebih rendah (P<0,05) jika dibandingkan dengan penggunaan aras 20% maupun 30%. Dapat disimpulkan bahwa penggunaan kuning telur itik memberikan hasil yang terbaik terhadap kualitas spermatozoa kambing Bligon, jika dilihat dari variable: motilitas, viabilitas, abnormalitas spermatozoasetelah pengenceran (masing-masing: 74,00; 97,60 dan 0,60%), ekuilibrasi (masing-masing: 72,20; 94,00 dan 1,90%), prefreezing (masing-masing: 56,80; 77,70 dan 5,40%) dan post-thawing (masing-masing: 43,60; 62,60 dan 8,50%).(Kata kunci: Kambing Bligon, Kuning telur itik, Pengenceran, Sperma, Tris fruktosa)
PRODUKSI DAN KOMPOSISI SUSU KAMBING PERANAKAN ETTAWA DI DATARAN TINGGI DAN DATARAN RENDAH DAERAH ISTIMEWA YOGYAKARTA Rian Rosartio; Yuni Suranindyah; Sigit Bintara; Ismaya (Ismaya)
Buletin Peternakan Vol 39, No 3 (2015): BULETIN PETERNAKAN VOL. 39 (3) OKTOBER 2015
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v39i3.7986

Abstract

THE PROPORTION OF X AND Y SPERM, VIABILITY AND MOTILITY OF RAM SPERMATOZOA AFTER SEPARATED WITH WHITE EGG ALBUMIN Moh Takdir; Ismaya ismaya; Sigit Bintara
Buletin Peternakan Vol 41, No 1 (2017): BULETIN PETERNAKAN VOL. 41 (1) FEBRUARI 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v41i1.9130

Abstract

ABSTRACT The aim of this research was to determine the proportion, viability and motility of X and Y ram spermatozoa separated with egg white albumin. Sperm samples derived from Garut ram, which was collected by using an artificial vagina. Observations were made on spermatozoa fraction above and below each medium fraction treatment. There are treatment egg white albumin as separation medium, each medium consisting of fractions top and bottom fraction with different concentration: 1) P0 = sperma before separation (control); 2) P1 = 10% above fraction + 30% lower fraction; P2 = 25% + 45%; P3 = 25% + 75%. Data proportion of X and Y, viability and motility were analyzed statistically by Completely Randomized Design patern in the direction followed by Duncan’s Multiple Range Test for data with a real difference. Separation with egg white albumin affect significantly increased the proportion of spermatozoa X and Y (P≤0.05), but tends to decrease the viability and motility of spermatozoa.The proportion of spermatozoa X and Y was highest in treatment P3,76.76% of spermatozoa X (fraction above 25%) and 79.81% spermatozoa Y (75% lower fraction), with an average viability obtained respectively 68,9% (fraction above) and 59,7% (bottom fraction), motility 77,5% (fraction above) dan 84,0% (bottom fraction). It was concluded that the egg white albumin is very effective in changing the proportions of X and Y ram sperm with the quality of spermatozoa after separation feasible for applications insemination or processed into frozen semen.  (Keywords: Garut ram, White egg albumin, Spermatozoa X and Y) 
Supplementation of Follicle Stimulating Hormon Into In vitro Maturation Medium to Increase Oocytes Maturation and 4 Cell Stadium Embryo Development of Bligon Goat Yanuar Achadri; Sigit Bintara; Diah Tri Widayati
Buletin Peternakan Vol 42, No 2 (2018): BULETIN PETERNAKAN VOL. 42 (2) MAY 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v42i2.13609

Abstract

The study was carried out to investigate the effect of follicle stimulating hormon (FSH) into in vitro maturation medium to increase oocytes maturation and 4 cell stadium embryo development of Bligon goat. Goat ovaries were obtained from a slaughterhouse and transported to the laboratory in a flask of NaCl at temperature of 31 – 34°C. Oocytes were aspirated from 2 – 6 mm of follicles into a 3 mL syringe (23G needle) that contained Dulbecco’s Phosphate-Buffered Saline. Oocytes were divided into three groups, i.e tissue culture medium (TCM) with FSH supplementation 0, 50, and 100 IU/mL. Oocytes were put into those medium and incubated on 39°C, 5% CO2, and 95% humidity for 24 hours. Matured oocytes were fertilized with capacitated frozen thawed-semen and incubated on 39°C, 5% CO2, and 95% humidity for 5 hours. Fertilized oocytes were washed for 3 times in TCM and incubated in the same condition for embryo culture. The data of FSH supplementation and embryo development were analyzed using randomized completely one way classification. The results showed that the percentages of mature oocytes from FSH supplementation 0, 50, and 100 IU/mL were 70,48±23,22, 78,48±15,80, and 80,29±12,86%, respectively. Cleavage rate of the two cells stage were 36,00±14,22, 44,00±33,94, and 57,45±31,78%, respectively, and for the 4 cells stage were 27,33±22,04, 35,33±40,73, and 39,45±20,38%. It is concluded that supplementation of FSH in the maturation medium could not increase the percentages of in vitro maturation and embryo development.
REPRODUCTIVE PERFORMANCES OF SUMBA ONGOLE COWS INSEMINATED WITH FROZEN BELGIAN BLUE SEMEN Riyan Nugroho Aji; Panjono Panjono; Ali Agus; Budi Prasetyo Widyobroto; Tety Hartatik; I Gede Suparta Budisatria; Ismaya Ismaya; Sigit Bintara
Buletin Peternakan Vol 41, No 4 (2017): BULETIN PETERNAKAN VOL. 41 (4) NOVEMBER 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v41i4.22052

Abstract

The purpose of this study was to observe the reproductive performances of Sumba Ongole (SO) cows inseminated with frozen Belgian Blue (BB) semen. This research was expected to be used as a reference in the cow’s maintenance, especially in the implementation of Artificial Insemination (AI), as well as for subsequent studies. Parameter of this study includes the signs of estrus symptoms, S/C ratio, CR, NRR, and pregnancy time. The results showed a large value of S/C for cow SO 2.60±1.81, great value for cattle CR SO was 40%, the value of NRR was 60%, and the pregnancy time SO was 273.80±4.08. It is concluded that reproductive performance of SO cows are good, using SO cows to cross with BB cow is possible to do. 
SRY Gene Marker Differences in Native and Crossbreed Cattle Tety Hartatik; Dwi Ahmad Priyadi; Ali Agus; Sigit Bintara; I Gede Suparta Budisatria; Panjono Panjono; Budi Prasetyo Widyobroto; Yudi Adinata
Buletin Peternakan Vol 42, No 3 (2018): BULETIN PETERNAKAN VOL. 42 (3) AUGUST 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v42i3.29940

Abstract

This study focused on the promoter region of the SRY gene with 1,281 bp DNA fragments, including 5'UTR, CAAT signal, and TATA box. Genomic samples of 19 cattle were obtained from Wagyu-BX (n = 2), BX (n = 5), Simmental (n = 2), Limousin (n = 2), Ongole (n = 2), Madura (n = 2), Bali (n = 2), Nellore (n = 1), and Hereford (n = 1). Two flanking primers (forward and reverse) were used for polymerase chain reaction (PCR). The PCR products were then sequenced by using a two-way primer. The obtained sequences were aligned with clustalW software to determine the differences in the nucleotide base arrangement which compiled the promoter region of the SRY gene. The cattle crossbreeding was done as an effort to improve the genetic variations and qualities. The SRY gene is a marker gene inherited from the male side (bull), so the SRY gene is expected to be used as a marker to monitor the crossbreeding. The monitoring of the crossbreed cattle is an initial effort to increase the genetic variations and enhance the genetic qualities without threatening the germplasm purity. The results of this study showed that the overall sample is monomorphic, except for Bali and Nellore cattle. Further research is needed by expanding the analysis area of the SRY gene and increasing the number of samples.
Co-Authors Achadiah Rachmawati Agustina, Rona Ajat Sudrajat Aji, Riyan Nugroho Aji, Riyan Nugroho Akhmad Fathoni Ali Agus Ali Agus Ali Agus Andriyani Astuti Budi Prasetyo Widyobroto Diah Tri Widayati Diah Tri Widayati Diahanvika Tri Sarvinda Diatmono, Dio Fico Felsidan Dwi Astuti Dyah Maharani Eka Rizky Vury Rahayu Endang Baliarti Fatimah Az Zahra Chairunissa Heru Sasongko I Gede Suparta Budisatria I Gede Suparta Budisatria I Gede Suparta Budisatria I Gede Suparta Budisatria I Gede Suparta Budisatria I Gede Suparta Budisatria Ikhwan Firhamsah Ismaya (Ismaya) Ismaya Ismaya Ismaya Ismaya Ismaya Ismaya Ismaya Ismaya Ismaya Ismaya Kumala, Seraphina Kustantinah Kustantinah Kustono (Kustono) Labib Abdillah, Labib Luis Tavares Moh Takdir Nurul Hidayat Padmawati, Fransisca Gani Prajaningrum Luluk Miftahul Umami Priyadi, Dwi Ahmad Raden Febrianto Chsristi Rian Rosartio Riyan Nugroho Aji Sastranegara, Arina Dinar Nurfadilla Seraphina Kumala Sitaresmi, Pradita Iustitia Sri Harimurti Tety Hartatik Tri Anggraeni Kusumastuti Widya Asmarawati Yanuar Achadri Yudi Adinata Yuni Suranindyah