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All Journal HAYATI Journal of Biosciences Buletin Peternakan
Sitaresmi, Pradita Iustitia
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Veterinary Other

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Genetic External Characteristics of Tukong: Indonesian Rumpless Indigenous Chicken Tribudi, Yuli Arif; Nurgiartiningsih, Veronica Margaretha Ani; Ulfah, Maria; Sari, Aprilianna Putri Zahara Nafsina Luvita; Hariyono, Dwi Nur Happy; Sitaresmi, Pradita Iustitia; Crooijmans, Richard PMA; Natsir, Muhammad Halim; Gusmalawati, Dwi; Ibrahim, Alek
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1147-1156

Abstract

Tukong chicken, also known as rumpless chicken due to the absence of a tailbone, is one of the indigenous chicken breeds in Indonesia currently at risk of extinction. Due to its scarcity, genetic information on the Tukong chicken remains limited, and its potential has not been fully explored. Therefore, this study aimed to determine the genetic external characteristics of the Tukong chickens. Data were collected from 205 chickens (52 males; 153 females) raised in Landak and Bengkayang Regency, West Kalimantan. The varieties are based on feather color, plumage color, feather pattern, feather flicker, comb shape, and shank color. The data were used to analyze the rate of the original gene, the frequency of autosomal genes, sex-linked genes, feather pattern, introgression rate, gene frequency, and expected heterozygosity. The majority of the Tukong chickens had colored feathers (88.78%), columbian feather type (47.80%), plain feather pattern (61.46%), silver feather flicker (54.63%), yellow-white shank (62.44%), and pea comb (77.07%), which were controlled by ii, ee, bb, SS, IdId, and P_ genes, respectively. The introgression rate from exotic breeds into Tukong chicken is 53.2%, with the original gene at 46.8%, while the average expected heterozygosity was 0.416±0.025, indicating a high level of genetic diversity of the traits. This observed variation in qualitative morphological characteristics might benefit both the conservation and utilization of the Tukong chicken.
Various Saccharides Addition of Extender Ram Efficiency to Improve Cryopreservation in Semen Etawah Crossbreed Bucks with Seminal Plasma Replacement nurlatifah, aeni; Sitaresmi, Pradita Iustitia; Hafid, Anita; Mahari, Desiana Ade; Lupitasari, Florentina Bety Indah; Anwar, Rahma Isartina; Santoso, Santoso; Rizal, Muhammad; Herdis, Herdis
Buletin Peternakan Vol 48, No 2 (2024): BULETIN PETERNAKAN VOL. 48 (2) MAY 2024
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v48i2.89390

Abstract

Phospholipase in buck seminal plasma will trigger a coagulation incident resulting in the demise of the spermatozoa during liquid semen processing using milk or egg yolk substrates diluent. A plasma replacement process is performed to avoid this possibility. The cryopreservation process leads to sperm cell damage due to the freezing process, therefore cryoprotectant agents such as saccharides are required as protective agents. This study aimed to investigate the effect of various types of saccharides on the quality of frozen semen of etawah crossbreed (PE) bucks during cryopreservation and thawing with plasma replacement using Priangan ram semen plasma. Semen was collected using an artificial vagina once a week. Fresh PE bucks semen centrifuged at 3,000 RPM for 30 min. The supernatant (seminal plasma) was removed and replaced in equal volume with Priangan ram seminal plasma. Semen was divided into four treatments: goat semen with sheep semen plasma in Tris diluent (control); control with 0.6% dextrose added (monosaccharides); control with 0.6% sucrose added (disaccharides); control with 0.6% raffinose added (trisaccharides). Semen quality including percentages of motile spermatozoa, live spermatozoa, and intact plasma membrane (IPM) were evaluated after diluted, equilibrated, and thawing, respectively. Results of this study showed that viability and IPM of monosaccharides, disaccharides, and trisaccharides were significantly (P<0.05) higher than control (54.40 and 51.40; 55.00 and 53.60; 55.60 and 52.20 vs 48.40 and 52.20, respectively). The motility of disaccharides was significantly (P<0.05) higher compared to control and other diluent (47.00 vs 41.00, respectively). In conclusion, different types of saccharides were effective in maintaining the quality of etawah crossbreed buck frozen semen. The addition of disaccharides seems more effective compared to monosaccharides and trisaccharides in tris extender on the quality of etawah crossbreed buck frozen semen.
Physicochemical and Sensory Properties of Goat Meat Sausages Prepared With Different Levels of Goat Liver Triyannanto, Endy; Rusman, Rusman; Cahyowati, Meireni; Kayihura, Joseph; Sitaresmi, Pradita Iustitia
Buletin Peternakan Vol 48, No 4 (2024): BULETIN PETERNAKAN VOL. 48 (4) NOVEMBER 2024
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v48i4.97221

Abstract

Anemia, a common issue among children and teenagers in Indonesia, is characterized by hemoglobin levels below normal. Iron is essential for producing hemoglobin in red blood cells, which helps prevent anemia. Innovation to provide a popular goat liver-based product that is convenient to consume and easily marketable underpins the development of this goat liver-enhanced sausage. The aim of this research was to determine the effect of partially substituting goat meat with different levels of goat liver on physicochemical and sensory properties of goat meat sausages. Sausages containing 100:0, 75:25, and 50:50 goat meat to goat liver ratios and goat meat (%, w/w) , and then added with other ingredients (tapioca flour, skim milk, garlic, pepper, coriander, salt, STPP, and ice water) were prepared and cooked for 30 minutes. The sausages were then analyzed for physical properties, including pH, texture and water holding capacity (WHC), iron content, and sensory quality. Data were analyzed using analysis of variance for a completely randomized design, and then if there were significant differences between means, they were tested using Duncan’s New Multiple Range Test. The results showed that substitution of goat liver significantly (p0.05). In conclusion, substituting up to 50% of goat meat with goat liver will improve the iron content of goat meat sausages with minimal impact on the sensory and proximate composition of the product.
The Influence of Parity on Blood Metabolite Profiles and Reproductive Performance in Saanen-Etawah Crossbred Does Diatmono, Dio Fico Felsidan; Paramita, Stefani Winda; Padmawati, Fransisca Gani; Sitaresmi, Pradita Iustitia; Kumala, Seraphina; Andi, Megawati; Widyobroto, Budi Prasetyo; Suranindyah, Yustina Yuni; Freitas, Joana da Costa; Widayati, Diah Tri
Buletin Peternakan Vol 49, No 4 (2025): BULETIN PETERNAKAN VOL. 49 (4) November 2025
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v49i4.110480

Abstract

This study aimed to observe the effect of parity on the blood metabolite profiles and reproductive performance of Saanen-Etawah Crossbred does, maintained in smallholder farm. A total of 40 non-pregnant lactating does were divided into two groups: primiparous (n=16) and multiparous (n=24). The blood metabolite profiles, including total protein, glucose, cholesterol, and blood urea nitrogen (BUN), were assessed. Blood samples were collected from the jugular vein during the follicular phase, two hours prior morning feeding. Analysis of blood metabolites was carried out using photometric atomic absorption spectroscopy (AAS)-flame procedure. Furthermore, reproductive performance was observed through observing the characteristics of estrus and the length of the estrus cycle. The results indicated total protein in blood were significantly higher (p<0.05) in multiparous does (7.04±0.87 g/dL) compared to primiparous does (5.47±1.40 g/dL). In contrast, primiparous does exhibited significantly higher (p<0.05) blood cholesterol levels (122.33±30.55 mg/dL) than multiparous does (107.14±13.45 mg/dL). Multiparous does displayed typical estrus characteristics, including vulvar reddening, swelling, and vaginal mucus production, alongside a significantly shorter estrus cycle length. Parity was positively correlated with total protein levels (r=0.577), vulvar reddening (r=0.355), vulvar swelling (r=0.343), and vaginal mucus production (r=0.450), while negatively correlated with cholesterol levels (r=-0.330) and estrus cycle length (r=-0.532). These findings indicate that parity influences total protein and cholesterol levels, as well as several key estrus characteristics. Further investigation into optimized feed regimens and reproductive hormone fluctuations is warranted to enhance the reproductive efficiency of Saanen-Etawah Crossbred does.
Co-Authors Alek Ibrahim Andi, Megawati Anwar, Rahma Isartina Budi Prasetyo Widyobroto Cahyowati, Meireni Crooijmans, Richard PMA Diah Tri Widayati Diatmono, Dio Fico Felsidan Dwi Gusmalawati Freitas, Joana da Costa Hafid, Anita Hariyono, Dwi Nur Happy herdis herdis Kayihura, Joseph Kumala, Seraphina Lupitasari, Florentina Bety Indah Mahari, Desiana Ade Maria Ulfah Muhammad Halim Natsir MUHAMMAD RIZAL Nurgiartiningsih, Veronica Margaretha Ani nurlatifah, aeni Padmawati, Fransisca Gani Paramita, Stefani Winda Rusman Rusman SANTOSO SANTOSO Sari, Aprilianna Putri Zahara Nafsina Luvita Triyannanto, Endy Yuli Arif Tribudi Yustina Yuni Surandiyah