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Histologi Jaringan Hepar Mencit (Mus Musculus) yang Difiksasi dengan Larutan Carnoy dengan Variasi Waktu 4 Jam, 8 Jam dan 12 Jam Arin Dwi Afrida; Djoko Priyatno
Jaringan Laboratorium Medis Vol 3, No 1 (2021): May 2021
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v3i1.7920

Abstract

Histopathological examination is a routine examination process for each tissue in the anatomic pathology laboratory. The stages in making histology preparations are fixation stages. The type of solution that can be used as a fixative solution other than 10% NBF is Carnoy's solution. Carnoy's solution is a fixative solution with a relatively fast fixation process of about 1-4 hours. The advantages of Carnoy's solution can lyse erythrocytes and dissolve lipids, have the ability to maintain cell nuclei, and retain glycogen. The purpose of this study was to determine the histology of the liver tissue preparations of mice (Mus Musculus) which were fixed using Carnoy's solution with time variations of 4 hours, 8 hours, and 12 hours. This is an observational research with descriptive research design criteria. The results of the histology of the liver tissue preparations of mice (Mus Musculus) which were fixed using the 10% NBF solution group obtained 100% good preparations. In the Carnoy solution group with a time of 4 hours, it gave 2.2% results of poor preparations and 97.78% of good preparations. While the treatment group with Carnoy's solution with a time of 8 hours obtained as many as 11.11% of preparations that were not good and 88.89% of good preparations. and in the Carnoy solution group with a time of 12 hours, there were 44.44% of the poor preparations and 55.56% of the good preparations.
Studi Literatur Gambaran C-Reactive Protein Pada Penderita Hipertensi Umi Fadilah; Djoko Priyatno
Jaringan Laboratorium Medis Vol 2, No 2 (2020): November 2020
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v2i2.7696

Abstract

Hypertension is called the silent killer, often known after complications occur. Based on the results of Riskesdas in 2018, the prevalence of hypertension in Indonesia at age ≥18 years increased to 34.1% in 2018. Endothelial injury caused by hypertension triggers inflammation or chronic inflammation of the arterial wall. Evidence of an inflammatory process is the discovery of C-Reactive Protein (CRP) which is a marker of inflammation from non-bacterial infections.The purpose of this study to knowing the description of C-Reactive Protein in patients with hypertension through research by Fadhliani (2016), Dolly (2018), Harahap (2019). This paper is a summary of  research result, with the criteria for systematic study research through a systematic review approach. In patients with hypertension according to research Fadhliani (2016) obtained as much as 23% C-Reactive Protein positive and 77% C-Reactive Protein negative. In the Harahap study (2019) of 30 people with hypertension, 12 people (40%) had positive C-Reactive Protein and 18 people (60%) had negative C-Reactive Protein. Whereas in the study of Dolly (2018) the average level of hs-CRP in hypertensive patients is 4, 85 mg / L. According to Dolly's research (2018) in patients with hypertension for 1 year have higher levels of hs-CRP compared to patients with hypertension for a period of ≤1 years. Examination of C-Reactive Protein (CRP) in patients with hypertension in the Harahap study (2019) showed positive CRP results dominated by subjects with age ≥ 50 years as many as 8 people (27%).
SKRINING DAN PENDAMPINGAN PENCEGAHAN PENYAKIT TIDAK MENULAR DI MASYARAKAT Nina Indriyawati; Widodo Widodo; Melyana Nurul Widyawati; Djoko Priyatno; Marichatul Jannah
Jurnal LINK Vol 14, No 1 (2018): MEI 2018
Publisher : Pusat Penelitian dan Pengabdian kepada Masyarakat, Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.22 KB) | DOI: 10.31983/link.v14i1.3287

Abstract

Increasing the incidence of PTM, it is necessary for education and assistance to the community to carry out early detection or screening of PTM, especially in groups at risk. The target of this community service activity is risky and elderly people as much as 150 people in Demak District, Gubug Sub-district Grobogan District, and District Pudak Payung. evaluation is done by providing a questionnaire about the level of knowledge and monitoring of laboratory results, and blood pressure. Evaluation will be measured from the level of knowledge before and after health education. Increased PTM can be suppressed through the control of risk factors, namely reduction of cigarette, alcohol, sugar and salt consumption, increased consumption of fruits and vegetables, increase physical activity through exercise, prevent obesity, stress control with recreational activities and check blood pressure, regular. Efforts to prevent PTM can be done by the community independently through Posbindu activities.