Garuda - Garba Rujukan Digital

Spontaneous cholangiohepatitis in broiler chickens: immunohistochemical study of Ito cells Handharyani, E; Ochiai, K; Winarsih, W; Harlina, E; Estuninngsih, Salfina Nurdin
Indonesian Journal of Animal and Veterinary Sciences Vol 6, No 4 (2001)
Publisher : Indonesian Animal Sciences Society

The function of Ito cells is expanding from a fat-storing site to a center of extracellular matrix metabolism and mediator production in the liver. Immunohistochemical reactivities of Ito cells were examined in eight livers of broiler chickens affected with spontaneous cholangiohepatitis and six chicken livers with malformation of extrahepatic biliary tracts. The livers in both groups revealed severe diffuse fibrosis. Ito cells expressing HHF35 muscle actin and desmin actively proliferated in the fibrotic foci of the all livers. The immunoreactivities of Ito cells to antibodies were enhanced compared with those in normal livers. There were no immunohistochemical differences between the Ito cells of two groups. From these findings, it was suggested that Ito cells actively proliferate and show enhanced immunoreactivities in the livers affected with cholangiohepatitis andmalformation of extrahepatic biliary tracts. Â Key words : Cholangiohepatitis, immunohistochemistry, ito cell, broiler chicken

Immunohistochemical study of Ito cells of spontaneous cholangiohepatitis in broiler chickens Handharyani, E; Ochiai, K.; Winarsih, W.; Harlina, E.; Estuninngsih, S.
Indonesian Journal of Animal and Veterinary Sciences Vol 6, No 4 (2001)
Publisher : Indonesian Animal Sciences Society

The function of Ito cells is expanding from a fat-storing site to a center of extracellular matrix metabolism and mediator production in the liver. Immunohistochemical reactivities of Ito cells were examined in eight livers of broiler chickens affected with spontaneous cholangiohepatitis and six chicken livers with malformation of extrahepatic biliary tracts. The livers in both groups revealed severe diffuse fibrosis. Ito cells expressing HHF35 muscle actin and desmin actively proliferated in the fibrotic foci of the all livers. The immunoreactivities of Ito cells to antibodies were enhanced compared with those in normal livers. There were no immunohistochemical differences between the Ito cells of two groups. From these findings, it was suggested that Ito cells actively proliferate and show enhanced immunoreactivities in the livers affected with cholangiohepatitis and malformation of extrahepatic biliary tracts.Key words: Cholangiohepatitis, immunohistochemistry, ito cell. broiler chicken

Heat stress effect and given of hexane extract jaloh bark (Salix tetrasperma Roxb) to cortisol level, triiodothyronine and hematology profile of broiler chickens ., Sugito; Manalu, W; Astuti, D.A; Handharyani, E; ., Chairul
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 3 (2007)
Publisher : Indonesian Animal Sciences Society

Plasma concentration of cortisol and hematological profile on broiler chickens can be used as heat stress indicators. This study was conducted to investigate the effect of administration of n-hexane extract of jaloh bark (EHJ) on heat-stressed broiler chicken. Thirty broilers, aged 20 days (strain Cobb), were randomly divided into 5 groups. The first group was external control (tCP) i.e. chickens without heat stress and without EHJ administration. The second group was internal control (CP) representing chickens given heat stress without EHJ administration. The third, fourth, andÂ fifth groups were chickens given heat stress and EHJ administration at doses 5, 10, and 20 mg/kg BW, respectively symbolized as CP+EHJ5, CP+EHJ10, and CP+EHJ20. The experimental broilers were exposed to heat stress in constant temperature of 33 Â± 1oC for 4 hours daily for 5 and 10 days. The EHJ was given 1 hour before temperature in the cage was raised and was given in daily basis.Â The level of cortisol in feces were measured from feces collected 1-2 hours beforeÂ treatment, 3-4 hours after heat stress exposure, and 2-3 hours after heat stress termination. This research indicated that heat stress at temperature 33 Â± 1oC during 2 and 4 hours increased cortisolÂ excretion in feces (P<0.05) and increased ratio H : L (P<0.10). The EHJ at dose of 10 mg/kg BW relatively gave more protection from heat stress impact on broilers. Key Words: Cortisol, Triiodothyronine, Heat Stress, Salix, Hematology

Histopathology of liver and kidney on broiler chicken exposed to heat stress and fed extract of Jaloh (Salix tetrasperma Roxb) ., Sugito; Manalu, W; Handharyani, E; ., Chairul
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 1 (2007)
Publisher : Indonesian Animal Sciences Society

This research was conducted to study the effects of jaloh extract (Salix tetrasperma Roxb) on broiler chicken exposed to heat stress in constant temperature of 33 Â± 1oC for 4 hours per day for 10 days. Fifteen chickens aged 20 days old (strain Cobb) were divided randomly into 5 groups. The first group was external control (tCP) of the chickens received neither heat stress nor jaloh extract. The second group was internal control (CP) representing chickens given heat stress without jaloh extract. The third, fourth, and fifth groups consisted of chickens given heat stress and 10 mg/kg BB of hexane (CP+Hek), ethyl acetate (CP+EtOAc), and ethanol (CP+EtOH) fractions, respectively. Heat stress and jaloh extract were given every day. Extract of jaloh were given 1 hour before temperature in the cage was raised. On 10th day of the experiment, 3 chickens were killed and necropted. Liver and kidney tissues from each treatment were analyzed for histological condition. The results indicated that heat stress can induce injury degeneration, necrosis, and infiltration of inflammation cells of liver and kidney. It is concluded that the hexane fraction of jaloh extract could protect the detrimental effects on heat stress. Key Words: Heat Stress, Extract Salix, Liver, Broiler Kidney

Heat stress effect and given of hexane extract jaloh bark (Salix tetrasperma Roxb) to cortisol level, triiodothyronine and hematology profile of broiler chickens Sugito .; W Manalu; D.A Astuti; E Handharyani; Chairul .
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 3 (2007): SEPTEMBER 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Plasma concentration of cortisol and hematological profile on broiler chickens can be used as heat stress indicators. This study was conducted to investigate the effect of administration of n-hexane extract of jaloh bark (EHJ) on heat-stressed broiler chicken. Thirty broilers, aged 20 days (strain Cobb), were randomly divided into 5 groups. The first group was external control (tCP) i.e. chickens without heat stress and without EHJ administration. The second group was internal control (CP) representing chickens given heat stress without EHJ administration. The third, fourth, and fifth groups were chickens given heat stress and EHJ administration at doses 5, 10, and 20 mg/kg BW, respectively symbolized as CP+EHJ5, CP+EHJ10, and CP+EHJ20. The experimental broilers were exposed to heat stress in constant temperature of 33 ± 1oC for 4 hours daily for 5 and 10 days. The EHJ was given 1 hour before temperature in the cage was raised and was given in daily basis. The level of cortisol in feces were measured from feces collected 1-2 hours before treatment, 3-4 hours after heat stress exposure, and 2-3 hours after heat stress termination. This research indicated that heat stress at temperature 33 ± 1oC during 2 and 4 hours increased cortisol excretion in feces (P<0.05) and increased ratio H : L (P<0.10). The EHJ at dose of 10 mg/kg BW relatively gave more protection from heat stress impact on broilers. Key Words: Cortisol, Triiodothyronine, Heat Stress, Salix, Hematology

Immunohistochemical study of Ito cells of spontaneous cholangiohepatitis in broiler chickens E Handharyani; K. Ochiai; W. Winarsih; E. Harlina; S. Estuninngsih
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 4 (2001): DECEMBER 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The function of Ito cells is expanding from a fat-storing site to a center of extracellular matrix metabolism and mediator production in the liver. Immunohistochemical reactivities of Ito cells were examined in eight livers of broiler chickens affected with spontaneous cholangiohepatitis and six chicken livers with malformation of extrahepatic biliary tracts. The livers in both groups revealed severe diffuse fibrosis. Ito cells expressing HHF35 muscle actin and desmin actively proliferated in the fibrotic foci of the all livers. The immunoreactivities of Ito cells to antibodies were enhanced compared with those in normal livers. There were no immunohistochemical differences between the Ito cells of two groups. From these findings, it was suggested that Ito cells actively proliferate and show enhanced immunoreactivities in the livers affected with cholangiohepatitis and malformation of extrahepatic biliary tracts.Key words: Cholangiohepatitis, immunohistochemistry, ito cell. broiler chicken

Histopathology of liver and kidney on broiler chicken exposed to heat stress and fed extract of Jaloh (Salix tetrasperma Roxb) Sugito .; W Manalu; E Handharyani; Chairul .
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 1 (2007): MARCH 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

This research was conducted to study the effects of jaloh extract (Salix tetrasperma Roxb) on broiler chicken exposed to heat stress in constant temperature of 33 ± 1oC for 4 hours per day for 10 days. Fifteen chickens aged 20 days old (strain Cobb) were divided randomly into 5 groups. The first group was external control (tCP) of the chickens received neither heat stress nor jaloh extract. The second group was internal control (CP) representing chickens given heat stress without jaloh extract. The third, fourth, and fifth groups consisted of chickens given heat stress and 10 mg/kg BB of hexane (CP+Hek), ethyl acetate (CP+EtOAc), and ethanol (CP+EtOH) fractions, respectively. Heat stress and jaloh extract were given every day. Extract of jaloh were given 1 hour before temperature in the cage was raised. On 10th day of the experiment, 3 chickens were killed and necropted. Liver and kidney tissues from each treatment were analyzed for histological condition. The results indicated that heat stress can induce injury degeneration, necrosis, and infiltration of inflammation cells of liver and kidney. It is concluded that the hexane fraction of jaloh extract could protect the detrimental effects on heat stress. Key Words: Heat Stress, Extract Salix, Liver, Broiler Kidney

Spontaneous cholangiohepatitis in broiler chickens: immunohistochemical study of Ito cells E Handharyani; K Ochiai; W Winarsih; E Harlina; Salfina Nurdin Estuninngsih
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 4 (2001): DECEMBER 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The function of Ito cells is expanding from a fat-storing site to a center of extracellular matrix metabolism and mediator production in the liver. Immunohistochemical reactivities of Ito cells were examined in eight livers of broiler chickens affected with spontaneous cholangiohepatitis and six chicken livers with malformation of extrahepatic biliary tracts. The livers in both groups revealed severe diffuse fibrosis. Ito cells expressing HHF35 muscle actin and desmin actively proliferated in the fibrotic foci of the all livers. The immunoreactivities of Ito cells to antibodies were enhanced compared with those in normal livers. There were no immunohistochemical differences between the Ito cells of two groups. From these findings, it was suggested that Ito cells actively proliferate and show enhanced immunoreactivities in the livers affected with cholangiohepatitis andmalformation of extrahepatic biliary tracts. Key words : Cholangiohepatitis, immunohistochemistry, ito cell, broiler chicken

Immunomodulatory effect of tea mistletoe (Scurrula oortiana) extract on chicken embryos S Murtini; R Murwanti; F Satrija; E Handharyani
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 3 (2006): SEPTEMBER 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Tea mistletoe is one of medicinal herb which believed has an anticancer activity, it’s due to the capability of immunostimulator. The following research was carried out to determine the immunomodulatory effect of tea mistletoe (Scurrula oortiana) extract on chicken embryos. Twenty White Leghorn Specific Pathogen Free (SPF) 10 days old embryonated chicken eggs were divided into four groups of 5 eggs. The first group served as control and they were inoculated with aquabidestilate sterile. The second, third and fourth group was inoculated with 0.1 mg, 0.2 mg, and 0.4 mg S. oortiana extract/egg respectively. S. oortiana extract was inoculated via allantoic cavity. All experimental eggs were incubated at 37oC until day 21 and incubation was terminated before the embryos hatched. The embryos and the lymphoid organs (bursa of Fabricius, thymus and spleen) were weighed. Immunomodulatory effect of tea mistletoe extract was measured by counting the percentage of bursa of Fabricius active lymphoid follicle and the area of thymus medulla. The results showed tea mislestoe extract at the dose of 0.1mg, 0.2 mg and 0.4 mg have immunomodulatory effect on chicken embryos indicated by the increase of percentage of active lymphoid follicle of bursa Fabricius i.e. 68.8, 71.8 and 57.8% and increase area of thymus medulla i.e. 24.9 – 39.3% respectively compared to control group i.e. 22.6% of active lymphoid follicle of bursa Fabricius and 17.6% of thymus medulla area. It is concluded that S. oortiana extract at the dose of 0.1mg, 0.2 mg and 0.4 mg have immunomodulatory effect on chicken embryos.Key Words: Scurrula oortiana, Tea Mistletoe, Embryonated Chicken Egg, Immunomodulator

Title

Found 9 Documents
Search
Journal : Jurnal Ilmu Ternak Veteriner

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 9 Documents Search Journal : Jurnal Ilmu Ternak Veteriner

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 9 Documents
Search
Journal : Jurnal Ilmu Ternak Veteriner