Article Per Year (5 Year)
p-Index From 2020 - 2025
1.151
P-Index
This Author published in this journals
All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Biotropika Research Journal of Life Science Jurnal Penelitian Pendidikan IPA (JPPIPA) Biopendix: Jurnal Biologi, Pendidikan dan Terapan
Nunung Harijati
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics

Published : 14 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : Jurnal Penelitian Pendidikan IPA (JPPIPA)

 1 
The Effect of Basal Medium on Callus Induction and Plant Regeneration in Anther Culture of Rice (Oryza sativa L.) Iriani, Yoanita Fadlilah; Prayantini, Dian Catur; Nahampun, Hartinio Natalia; Mastuti, Retno; Harijati, Nunung
Jurnal Penelitian Pendidikan IPA Vol 11 No 8 (2025): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i8.11884

Abstract

Anther culture is an effective tissue culture technique for rapidly producing doubled haploid (DH) plants, significantly shortening the breeding cycle by 2-3 years compared to conventional methods. This study aimed to evaluate the effects of callus induction basal medium (B5 Gamborg and N6 Chu) and subsequent plant regeneration in both Japonica and Indica rice genotypes. The experiment was conducted using a completely randomized design (CRD) with two treatment factors. The first factor was genotype (G), comprising Nipponbare (G1), RCKJ 05 (G2), RCKJ 10 (G3), RCKJ 15 (G4), RCKJ 25 (G5). The second factor was the basal medium (M), consisting of N6 Chu medium (M1) and B5 Gamborg medium (M2). Each treatment combination (genotype x medium) was replicated three times, with one Petri dish (containing 125 anthers) per replicate. Parameters observed included callus induction frequency (CIF), green plantlet regeneration (RGP), and albino plantlet regeneration (RAP). The results demonstrated that both genotype and the basal medium for callus induction significantly influenced callus induction frequency and green plantlet regeneration.
Co-Authors Budi Waluyo Chaireni Martasari Dian Flowrenzhy Estri Laras Arumingtyas Febriyanti, Vera Flowrenzhy, Dian Hermalina Sinay Iriani, Yoanita Fadlilah Nahampun, Hartinio Natalia Nurul Chairiyah Prayantini, Dian Catur Retno Mastuti Retno Mastuti Rodiyati Azrianingsih Rodiyati Azrianingsih Savitri Nurlaila Serafinah Indriyani Sri Widyarti Wahyu Widoretno Wahyu Widoretno Yasminatul Khoiroh Yasminatul Khoiroh