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Journal : AQUATIC SCIENCE

The size variation of rotifer Brachionus rotundiformis cultivated with different feed at 40 ppt salinity Rimper, Joice R.T.S.L; Harikedua, Silvana D; Warouw, Veibe
AQUATIC SCIENCE & MANAGEMENT Vol 7, No 1 (2019): APRIL
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.7.1.2019.25043

Abstract

Title (Bahasa Indonesia): Variasi ukuran rotifer Brachionus rotundiformisyang diberi pakan berbeda pada salinitas 40 ppt RotiferBrachionus rotundiformisis a group of zooplankton which is used by fish larvae for feeding to initiate their growth. This zooplankton is widely favored by marine fauna larvae because of its small size can fits well with various larval mouth; thus, it is easily preyed by larvae. This study aimed to determine the variation of rotifer B. rotundiformismorphometry if cultured with different feed at 40 ppt. The use of 40 ppt salinity is expected to provide a variable morphometric size because B. rotundiformishas a polymorphism property. Microalgae used as feed for rotifer B. rotundiformiswere Prochloronsp. and Nanochloropsis oculata. Microalgae were cultured with Hirata medium. In the early stages, B. rotundiformiswas cultured at optimum temperature (28 ºC) and salinity 20 ppt, then it was cultured at salinity 40 ppt. Salinity adaptation was done by raising the salinity of the medium by 2 ppt every two days in a 10 ml reaction tube containing 10 individuals. After adaptation, B. rotundiformiswas transferred in a 1000 ml container with a density of 50 individuals. For the morphometric aspect, the total length, the length of the lorica, the width of the lorica and the anterior width were measured. The result showed the morphometric of rotiferB. rotundiformisfed with microalgae Prochloronsp. at 40 ppt salinity was smaller than that of the rotifer fed with N. oculata. Based on that finding it can be concluded that B. rotundiformis fed with Prochloronsp. at a salinity of 40 ppt has the potential to be developed as feed for fish larvae. Further investigations on how to accelerate the cultivation of microalgae Prochloronsp. as feeding for B. rotundiformisare needed.RotiferBrachionus rotundiformismerupakan golongan zooplankton yang digunakan sebagai makanan bagi larva ikan. Zooplankton ini banyak disukai oleh larva fauna laut, karena ukurannya kecil yang cocok dengan berbagai bukaan mulut larva. Penelitian ini bertujuan untuk mengetahui variasi morfometri rotifer B. rotundiformis, jika dikultur pada salinitas yang tinggi (40 ppt) dengan pemberian pakan berbeda. Penggunaan salinitas 40 ppt diharapkan bisa memberikan ukuran morfometrik yang bervariasi, karena rotifer jenis ini memiliki sifat polimorfisme. Alga mikro yang digunakan sebagai pakan adalah Prochloronsp. Dan Nanochloropsis oculata.Alga mikro tersebut dikultur dalam media Hirata. Pada tahap awal, B. rotundiformisdikultur pada suhu optimum (28 ºC) dengan salinitas 20 ppt; kemudian, dikultur pada salinitas 40 ppt. Adaptasi salinitas dilakukan dengan menaikkan salinitas medium sebanyak 2 ppt setiap dua hari dalam tabung reaksi berukuran 10 ml, yang berisi 10 individu. Setelah diadaptasikan, rotifer dipindahkan ke wadah berukuran 1000 ml dengan kepadatan sebanyak 50 individu dan dikultur pada salinitas 40 ppt. Aspek morfometri berupa panjang total, panjang lorica, lebar lorica, dan lebar anterior diukur. Hasil penelitian menunjukkan, bahwa panjang total rotifer B. rotundiformis, yang diberi pakan Prochloronsp. berukuran lebih kecil dibandingkan dengan rotifer yang diberi pakan N. oculata. Berdasarkan hasil penelitian dapat disimpulkan, bahwa B. rotundiformisyang diberi pakan Prochloronsp. pada salinitas 40 ppt memiliki potensi untuk dikembangkan sebagai bahan pakan bagi larva ikan. Penelitian lebih lanjut tentang cara mempercepat budidaya microalgae Prochloronsp. sebagai makan untuk B. rotundiformis diperlukan.
First record of semi-aquatic bugs (Hemiptera: Veliidae) from Tasik Ria coastal waters, North Sulawesi, Indonesia Warouw, Veibe; Rimper, Joice R.T.S.L; Harikedua, Silvana Dinaintang; Manembu, Indri Shelovita; Rumampuk, Natalie Detty
AQUATIC SCIENCE & MANAGEMENT Vol. 13 No. 2 (2025): OCTOBER
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.v13i2.66637

Abstract

Semi-aquatic bugs of the family Veliidae (Hemiptera: Heteroptera: Gerromorpha) are primarily associated with freshwater habitats; their occurrence in coastal environments remains poorly documented, particularly in tropical regions. This study reports the first record of Veliidae from the coastal waters of Tasik Ria Beach, North Sulawesi, Indonesia, thereby expanding the known ecological distribution of this family. Specimens were collected from surface-water microhabitats using a sweep net, and key environmental parameters were measured in situ. Morphological identification was based on stereomicroscopic examination of diagnostic characters. Molecular confirmation was obtained through DNA barcoding of the mitochondrial cytochrome c oxidase subunit I (COI) gene, with sequences compared against the GenBank and BOLD databases. Both morphological and molecular analyses confirmed the specimens' taxonomic placement within Veliidae. Their presence in a coastal habitat indicates greater ecological plasticity and habitat tolerance than previously recognized. This finding establishes important baseline data for future research on the distribution, bioecology, and adaptive strategies of semi-aquatic insects in tropical coastal ecosystems.