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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences Bioma : Berkala Ilmiah Biologi JURNAL ANATOMI FISIOLOGI Jurnal Akademika Biologi Waste Technology NICHE Journal of Tropical Biology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Indonesian Journal of Limnology
Riche Hariyati
Departemen Biologi - FSM Universitas Diponegoro
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Earth & Planetary Sciences Education Environmental Science Health Professions Medicine & Pharmacology Public Health Other

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KEANEKARAGAMAN JENIS IKAN TANGKAP DI SEKITAR KARAMBA DAERAH NGASINAN WADUK KEDUNGOMBO JAWA TENGAH Anang Yunianto; Sapto Punomo Putro; Riche Hariyati
Jurnal Akademika Biologi Vol. 1 No. 1 Oktober 2012
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (94.114 KB)

Abstract

Nowadays the fish farming activity in the Kedungombo reservoir tends to increase gradually. The knowledge of diversity and abundance of  fish species would be usefull to preserve the ecosystem of the reservoir. The improving management of water resources reservoirs is also needed to increase the economy of local community. The purpose of this study is to determine the diversity and abundance of fish found in the area around Kedungombo reservoir. The study is explorative. Sampling was done randomly at 5 stations. Data analysis was done to determine the similiaritas index (Di), diversity index (H '), evenness index (e), relative abundance. Hutcheson t test was carried out to determine differences in species diversity at July and August with five-point observation stations.  Based on the results of the study, during the months of July to August, there are 8 species, namely: Silver barb (Barbonymus gonionotus), tilapia (Oreochromis niloticus), Mujair (Oreochromis mossambicus), catfish (Pangasius sutchi), Betutu (Oxyeleotris marmorata), Red Devil (Amphilophus labiatus), Redoson (Trichogaster trichopterus) and Gurame (Osphronemus goramy). The level of diversity and evenness of fish are considerably low. Silver barb fish dominated in relative abundance. Hutcheson t test results showed no difference in fish species diversity, indicated by t count <t table at the 95% confidence level. Water quality, light brightness and sediments were considered as a suitable habitat for fish. This study implies that other studies at the other regions are needed to assess the diversity of tropical fish
STRUKTUR KOMUNITAS PLANKTON DI KAWASAN WANA WISATA CURUG SEMIRANG KECAMATAN UNGARAN BARAT, SEMARANG Delliana Ratna Sari; Jafron Wasiq Hidayat; Riche Hariyati
Jurnal Akademika Biologi Vol. 6 No. 2 April 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Objective of the study was to know the structure of plankton community and to know the influential physical and chemical factors in the structure of plankton community in Semirang. This research was conducted on 6 stations with different water body condition. Results showed that the number of plankton species obtained were 12 species in the Bacillariophyta Division. Plankton species that widely found were Achnanthes sp, Cymbella tropica and Cocconeis pediculus. The relative abundance index categorized into the dominant species category. The plankton diversity index were mostly included in the moderate category. Similarity index belongs to the category of even distribution, none dominance of a kind. The similarity index is mostly included in the high category. Physical and chemical factors in these waters have good water quality for plankton life.  Keywords: plankton community structure, freshwater, ecotourism waterfall Semirang 
Community structure of algae epiphytic in different leaf litter of mangroves Riche Hariyati; Sri Widodo Agung Suedy; Rully Rahadian
NICHE Journal of Tropical Biology Vol. 1, No. 2, Year 2018
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.685 KB) | DOI: 10.14710/niche.1.2.30-34

Abstract

Mangrove is a plant that has the morphological and physiological adaptations to habitats which influenced by a tide. This plant can grow and develop in an environment of high salinity and its soil conditions are less stable and aerobic. Litter mangrove is the main source for the availability of detritus and is the largest component constituent of a food web in a mangrove ecosystem environment. Algae epiphytic microalgae is a group that lives attached to the plant, including the mangrove litter. The study aims to assess the abundance and diversity of epiphytic algae on different types of mangrove leaf litter. The observed mangroves leaf litter were Avicennia alba, Sonneratia sp. and Rhizophora sp. Epiphytic algae were brushed on mangrove leaf litter covering an area of 6.25 cm2 and diluted with distilled water. Samples were observed under a microscope with a magnification of 400 x. Water quality parameters were measured in situ and the organic ingredients, total N and P were analyzed. The results showed that epiphytic algae on each type of mangrove leaf litter were not different. Rhizophora sp is the most abundant and the richest species compared with other types of litter that are equal to 622 individuals / cm2 and 26 species of epiphytic algae. While the abundance of Avicennia alba and Sonneratia sp were 459 ind/cm2 and 321 ind /cm2, in the coastal mangrove areas of Morodemak was found 28 species of epiphytic algae. The common epiphytic algae species found in all types of mangrove litter were Cymbella sp., Coscinodiscus sp., and Synedra ulna. In general, the group of Bacillariophyta (diatoms) is the dominant species in some mangrove leaf. 
Pengaruh Salinitas dan Limbah Cair Tahu pada Konsentrasi yang Berbeda terhadap Pertumbuhan Mikroalga Botryococcus braunii Kutzing Savira Amelia Putri; Riche Hariyati; Tri Retnaningsih Soeprobowati
Buletin Anatomi dan Fisiologi Volume 6, Nomor 2, Tahun 2021
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.6.2.2021.161-166

Abstract

Pertumbuhan mikroalga Botryococcus braunii Kutzing dipengaruhi oleh faktor lingkungan berupa salinitas, suhu, pH, oksigen terlarut (DO), intensitas cahaya dan nutrisi pada media kultivasi. Penelitian ini bertujuan untuk mengkaji pengaruh salinitas dan limbah cair tahu terhadap pertumbuhan mikroalga B. braunii. Perlakuan yang dilakukan dalam penelitian ini yaitu konsentrasi limbah cair tahu 10% dengan salinitas 15‰ (L10S15); limbah cair tahu 10% dengan salinitas 20‰ (L10S20); limbah cair tahu 10% dengan salinitas 25‰ (L10S25); limbah cair tahu 20% dengan salinitas 15‰ (L20S15); limbah cair tahu 20% dengan salinitas 20‰ (L20S20); limbah cair tahu 20% dengan salinitas 25‰ (L20S25); limbah cair tahu 30% dengan salinitas 15‰ (L30S15); limbah cair tahu 30% dengan salinitas 20‰ (L30S20); dan limbah cair tahu 30% dengan salinitas 25‰ (L30S25). Pengamatan yang dilakukan meliputi kepadatan sel dan faktor lingkungan berupa salinitas, suhu, pH, oksigen terlarut, serta kadar N dan P total. Data yang diperoleh diolah menggunakan metode analisis sidik ragam (ANOVA) yang dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan kepadatan sel tertinggi didapatkan pada perlakuan L10S25 dengan rata-rata kepadatan sel 313x104sel/ml. Secara umum, semakin tinggi limbah cair tahu yang diberikan maka kepadatan sel mikroalga akan semakin rendah.
The Comparison of Different Diatom Digestive Method using HCl-H2O2 and HCl -KMnO4 in Telaga Pengilon Dieng Christopher Hardian Widyanta; Tri Retnaningsih Soeprobowati; Jumari Jumari; Riche Hariyati
Indonesian Journal of Limnology Vol. 1 No. 1 (2020): Indonesian Journal of Limnology
Publisher : Indonesian Society of Limnology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.792 KB) | DOI: 10.51264/inajl.v1i1.5

Abstract

Identification diatom has been applied as an important key to tracing paleoenvironmental conditions. The knowledge for diatom extraction from sediment is continuing, but the diatom digestive methods in the tropical area is still limited. Telaga Pengilon, located in Dieng Indonesia, is rich in organic material in the sediment, the sample also associates with epiphytic and benthic diatom. Therefore, extracting pure diatom frustules free of organic matter from the sediments is essential for this application. To find the appropriate method for extracting diatoms in Telaga Pengilon, this research compares HCl with H2O2 and HCl with KMNO4 as the digestive reagent. Based on the statistical result (HCl, KMnO4) less time consumed than (HCl, H2O2) during the digestive process (p<0.05) and produced the same amount of residue (p> 0.05). For Pinnularia and Frustulia, the majority of diatoms could be extracted using both methods. Under microscopy, the structure of diatoms remained almost perfect after digestion with H202, the striae clearly visible and the residue digested correctly. Another method using HCl, KMnO4 fragmentation was found for some Pinnularia, and for Frustulia, the striae are clearly visible. This study demonstrated that different diatoms have different resistance for reagents and some of the locations need different reagents because different materials are contained in the sediment and need help to remove material organically.  As far as the HCl and H2O2 are appropriate digestion methods in a tropical area such as Telaga Pengilon, HCl, KMnO4 can be a substitute for the less time-consuming process.
Utilizing Environmental DNA to Identify Eukaryotic Diversity in Mangrove Sediments at Demak, Central Java, Indonesia Cahyani, Ni Kadek Dita; Jumari, Jumari; Hariyati, Riche; Rahadian, Rully; Rahim, Aulia; Zega, Enita Setiawati; Kholifah, Rizqi Widya Nur; Barber, Paul Hendry; Putro, Sapto Purnomo; Soeprobowati, Tri Retnaningsih
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 29, No 4 (2024): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.29.4.543-556

Abstract

The mangrove ecosystem, found along tropical and subtropical coasts, adapts to extreme conditions like rapid tidal changes, high salinity, anthropogenic influences, and anoxic environments. Mangrove sediments host diverse organisms, particularly invertebrates and bacteria, which significantly influence sediment structure and biochemical processes by enhancing permeability and water flow. Modern molecular approaches, notably Next-Generation Sequencing (NGS), are increasingly used to identify macro and microorganism communities in sediments. NGS, a powerful tool for DNA and RNA sequencing, allows for parallel sequencing of numerous DNA fragments, providing comprehensive insights into genome structure, genetic variants, gene expression, and epigenetic modifications. Its efficiency and cost-effectiveness make NGS vital for both basic biological research and clinical diagnostics. Recent NGS studies on mangrove sediments have focused on bacterial, archaeal, and fungal diversity. The study examines eukaryotic diversity in mangrove sediments at two locations, targeting the Cytochrome Oxidase Subunit I (COI) gene, a universal marker for eukaryotes. Results indicate distinct taxa at each site with minimal overlap, demonstrating eDNA's potential for assessing both macro and microorganism diversity in mangrove sediments. This preliminary study underscores the utility of molecular techniques in biodiversity research and also dynamic ecosystem changes in the mangrove sediment ecosystem. The high influence of the environment around the mangrove ecosystem will affect the quality of the mangrove itself. eDNA here provides a fast method for recording possible changes to be able to carry out better management in the future.
Co-Authors Ahmad Yusuf Afandi Anang Yunianto Barber, Paul Hendry Christopher Hardian Widyanta Delliana Ratna Sari Delliana Ratna Sari Erry - Wiryani Erry Wiryani Erry Wiryani Fatmawati Munir Fitriyanto, Eko Bambang Hermawan Setiyo Budi Hartanto Indah Riasih Umami Jafron Wasiq Hidayat Jumari Jumari Jumari Jumari, Jumari Kenanga Sari Kholifah, Rizqi Widya Nur Maya Evita, Isnaini Nurul Muhammad Sindhunata Prambodo, Muhammad Sindhunata Ni Kadek Dita Cahyani, Ni Kadek Dita Prahmawaty, Raden Faradhiva Rahim, Aulia Reka Hafizhah, Reka Rully Rahadian Rully Rahadian Sapto P Putro Sapto punomo Putro Savira Amelia Putri Siti Chotidjah Sri Purwanti Sri Utami Sri Widodo Agung Suedy Syarif Prasetyo Tri Retnaningsih Soeprobowati Tri Retnaningsih Soeprobowati Tri Retnaningsih Soeprobowati Tri Retnaningsih Suprobowati Trian Septa Wijaya Yunita Kus Astuti Zega, Enita Setiawati