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All Journal Pharmaciana: Jurnal Kefarmasian Indonesian Journal of Cancer Chemoprevention Majalah Obat Tradisional Acta Pharmaciae Indonesia : Acta Pharm Indo Molekul: Jurnal Ilmiah Kimia JKKI : Jurnal Kedokteran dan Kesehatan Indonesia
. Harwoko
Department Of Pharmacy, Faculty Of Health Sciences, Universitas Jenderal Soedirman, Purwokerto, Indonesia
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Health Professions Medicine & Pharmacology Public Health

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Molecular Networking Analysis and Antibacterial Potential of Ethyl Acetate Extracts of Sinomicrobium sp. PAP.21 using OSMAC Method Harwoko, Harwoko; Rahmawati, Anggun Tri; Riyanti, Riyanti
Majalah Obat Tradisional Vol 29, No 2 (2024)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.92311

Abstract

Challenges in drug discovery include biosynthetic gene clusters which remain silent under standard laboratory culture conditions. On the other hand, the rediscovery of the known compounds is inevitable. Accordingly, One Strain-MAny Compounds (OSMAC) approach and molecular networking analysis are currently applicable to discovering new bioactive compounds. Sinomicrobium sp. PAP.21 isolated from marine sediment collected in Cenderawasih Bay, West Papua, was added to the culture. Then, the bacterium was cultured in five different liquid media (RL1, A1BFe+C, NB, LB, and seawater) and incubated for 4, 5, and 7 days. The bacterial cultures were extracted using ethyl acetate (EtOAc) separately for each medium and incubation period, followed by LC-HRMS measurement. A total of 45 ethyl acetate extracts were assayed for in vitro antibacterial activity against Micrococcus luteus and Escherichia coli. Molecular networking analysis through GNPS indicated that three putative compounds possess antibacterial properties. EtOAc extracts from the A1BFe+C medium demonstrated antibacterial activity against M. luteus. However, none of them were active against E. coli. Collectively, Sinomicrobium sp. PAP.21 produced bioactive compounds exhibiting antibacterial potential, particularly against Gram-positive bacteria.
Co-Authors . Septiyaningsih A. Haris Budi Widodo Azhar, Ade Rizki Azhar, Ade Rizkia Choironi, Nur Amalia Esti Dyah Utami Farah Amiria, Farah Fareza, Muhamad Salman Georgios Daletos Hanif Nasiatul Baroroh HENY EKOWATI Jeanita, Rani Saskia Jeanita, Rani Saskia Jungho Lee Maliana, Dina Michael Feldbrügge Nuryanti Nuryanti Peter Proksch Rahmawati, Anggun Tri Rainer Kalscheuer Riyanti Riyanti Sunarto, Sunarto Warsinah Warsinah