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All Journal Pharmaciana: Jurnal Kefarmasian Indonesian Journal of Cancer Chemoprevention Majalah Obat Tradisional Acta Pharmaciae Indonesia : Acta Pharm Indo Molekul: Jurnal Ilmiah Kimia JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Journal of Fisheries & Marine
. Harwoko
Department Of Pharmacy, Faculty Of Health Sciences, Universitas Jenderal Soedirman, Purwokerto, Indonesia
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Health Professions Medicine & Pharmacology Public Health

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Molecular Networking Analysis and Antibacterial Potential of Ethyl Acetate Extracts of Sinomicrobium sp. PAP.21 using OSMAC Method Harwoko, Harwoko; Rahmawati, Anggun Tri; Riyanti, Riyanti
Majalah Obat Tradisional Vol 29, No 2 (2024)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.92311

Abstract

Challenges in drug discovery include biosynthetic gene clusters which remain silent under standard laboratory culture conditions. On the other hand, the rediscovery of the known compounds is inevitable. Accordingly, One Strain-MAny Compounds (OSMAC) approach and molecular networking analysis are currently applicable to discovering new bioactive compounds. Sinomicrobium sp. PAP.21 isolated from marine sediment collected in Cenderawasih Bay, West Papua, was added to the culture. Then, the bacterium was cultured in five different liquid media (RL1, A1BFe+C, NB, LB, and seawater) and incubated for 4, 5, and 7 days. The bacterial cultures were extracted using ethyl acetate (EtOAc) separately for each medium and incubation period, followed by LC-HRMS measurement. A total of 45 ethyl acetate extracts were assayed for in vitro antibacterial activity against Micrococcus luteus and Escherichia coli. Molecular networking analysis through GNPS indicated that three putative compounds possess antibacterial properties. EtOAc extracts from the A1BFe+C medium demonstrated antibacterial activity against M. luteus. However, none of them were active against E. coli. Collectively, Sinomicrobium sp. PAP.21 produced bioactive compounds exhibiting antibacterial potential, particularly against Gram-positive bacteria.
Optimization of Secondary Metabolite Production in the Sponge Associated Bacterium Roseivirga sp. PAP.19 Using the OSMAC Approach Pradani, Andin Syamsi; Harwoko, Harwoko; Meinita, Maria Dyah Nur; Riviani, Riviani; Mettal, Ute; Riyanti
Jurnal Ilmiah Perikanan dan Kelautan 2025: IN PRESS ISSUE (JUST ACCEPTED MANUSCRIPT, 2025)
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

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Graphical Abstract  Highlight Research Compounds produced by Roseivirga PAP.19 bacteria have potential as antimicrobials. The OSMAC approach was used successfully to activate BGCs of Roseivirga PAP.19. The use of medium in bacterial culture affects the secondary metabolites produced by Roseivirga PAP.19 bacteria. Bacteria cultured in A1Bfe+C medium produced more secondary metabolites compared to the other medium. The different incubation times during culture affect the amount of secondary metabolite compounds produced.   Abstract Marine sponge-associated bacteria are a rich source of bioactive metabolites. The biochemical interaction between bacteria and sponges enables symbiotic bacteria to produce metabolites similar to those of their host. Metabolite extracts from symbiotic bacteria contain bioactive compounds with cytotoxic, antiviral, and antimicrobial properties. There is an urgent need for new antimicrobial agents due to the increasing resistance to existing drugs. This study aims to optimize the medium and incubation time of secondary metabolites produced by the symbiotic bacteria of the sponge Roseivirga sp. PAP.19. To stimulate biosynthesis of silent gene clusters, the One Strain Many Compounds (OSMAC) approach was used. Bacteria were cultured in various media and at different incubation times. This has the potential to increase the production of secondary metabolites. Secondary metabolites were analyzed using LC-HRMS. The potential of these metabolites as antimicrobial agents was subsequently evaluated. Results showed six compounds with antimicrobial potential. Notably, the A1BFe+C medium produced more bioactive compounds than the other medium, and the highest compound production occurred on day 4. These findings suggest that optimizing culture conditions using the OSMAC approach can enhance the yield of potentially valuable antimicrobial secondary metabolites from symbiotic bacteria, which may contribute to the discovery of new antimicrobial agents.
Co-Authors . Septiyaningsih A. Haris Budi Widodo Azhar, Ade Rizki Azhar, Ade Rizkia Choironi, Nur Amalia Esti Dyah Utami Farah Amiria, Farah Fareza, Muhamad Salman Georgios Daletos Hanif Nasiatul Baroroh Heny Ekowati Jeanita, Rani Saskia Jeanita, Rani Saskia Jungho Lee Maliana, Dina Meinita, Maria Dyah Nur Mettal, Ute Michael Feldbrügge Nuryanti Nuryanti Peter Proksch Pradani, Andin Syamsi Rahmawati, Anggun Tri Rainer Kalscheuer Riviani Riviani, Riviani Riyanti Riyanti Riyanti Sunarto, Sunarto Warsinah Warsinah