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All Journal Indonesian Journal of Cancer Chemoprevention Chimica et Natura Acta Acta Pharmaciae Indonesia : Acta Pharm Indo Jurnal Ilmiah Kesehatan Masyarakat : Media Komunikasi Komunitas Kesehatan Masyarakat Pharmacy Reports Molekul: Jurnal Ilmiah Kimia
Nur Amalia Choironi
Fakultas Farmasi, Universitas Gadjah Mada, Yogyakarta.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Eksplorasi Fungi Endofit Umbi Lapis Bawang Merah (Allium cepa) sebagai Antifungi dan Antikolesterol CHOIRONI, NUR AMALIA; SOETOMO, SUNARTO; BAROROH, HANIF NASIATUL
Acta Pharmaciae Indonesia Vol 6 No 1 (2018): Acta Pharmaciae indonesia Vol.6 No.1 Tahun 2018
Publisher : Pharmacy Department, Faculty of Health Sciences, Jenderal Soedirman University, Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (295.746 KB)

Abstract

Fungi endofit berpotensi sebagai alternatif pengobatan karena menghasilkan senyawa yang sama dengan tumbuhan inangnya. Allium cepa memiliki manfaat sebagai antifungi dan antikolesterol. Tujuan penelitian ini adalah isolasi, identifikasi, serta melakukan uji antifungi dan antikolesterol. Isolat yang diperoleh diidentifikasi secara makroskopik dan mikroskopik. Metode uji antifungi terhadap Candida albicans adalah metode difusi agar dan metode uji antikolesterol menggunakan rancangan acak lengkap (pre and posttest control design). Hasil isolasi fungi endofit diperoleh isolat FEAC 1 dan FEAC 2. Hasil penelitian menunjukkan bahwa FEAC 2 memiliki zona hambat sebesar 7,389 mm dan menurunkan kolesterol total secara signifikan serta meningkatkan kolesterol HDL.
Evaluation of natural compounds as VEGFR-2 inhibitors for breast cancer therapy: insights from molecular docking and drug-likeness analysis Aprilia, Vika; Sarmoko; Fareza, Muhamad Salman; Baroroh, Hanif Nasiatul; Choironi, Nur Amalia
Pharmacy Reports Vol. 4 No. 2 (2024): Pharmacy Reports
Publisher : Indonesian Young Scientist Group and UPN Veteran Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51511/pr.82

Abstract

Breast cancer remains one of the most common cancers worldwide, with VEGFR-2 (KDR) playing a key role in tumor angiogenesis. Inhibiting VEGFR-2 is a promising therapeutic strategy. Natural compounds are increasingly studied for their potential to inhibit VEGFR-2. This study aims to assess the binding affinity of 11 natural compounds (andrographolide, alpha-mangostin, pinostrobin, pinocembrin, ethyl-p-methoxycinnamate (EPMS), xanthorrhizol, galangin, gamma-mangostin, curcumin, cinnamaldehyde, and alashanoid B) to the VEGFR-2 protein through molecular docking and Lipinski's rule analysis, identifying promising candidates for breast cancer treatment. Molecular docking simulations were performed for 11 compounds and sunitinib as a control, with binding energies and interactions analyzed. The compounds were also evaluated for drug-likeness using Lipinski’s rule of five. Curcumin showed the highest binding affinity to VEGFR-2 with a binding energy of -9.9 kcal/mol, surpassing sunitinib (-9.4 kcal/mol). Key interactions were observed with active site residues Cys919 and Asp1046. All tested compounds met the criteria for oral bioavailability per Lipinski’s rules. Curcumin demonstrates potential as a VEGFR-2 inhibitor due to its favorable binding affinity and drug-like properties. Enhancing curcumin’s bioavailability is recommended for effective therapeutic application.
Bioinformatics Analysis of Rho GTP-ase Activating Protein 35 (ARHGAP35) in Breast Cancer Migration Febrian, Dicky Rizky; Setyono, Joko; Fareza, Muhamad Salman; Choironi, Nur Amalia; Fadlan, Arif; Sarmoko, Sarmoko
Indonesian Journal of Cancer Chemoprevention Vol 12, No 3 (2021)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev12iss3pp161-169

Abstract

Breast cancer is a second deadly cancer after lung cancer worldwide. Progression of cancer is driven by mutated cancer drive gene such as ARHGAP35. This study aims to analyze the role of ARHGAP35 in the growth and development of breast cancer cells. ARHGAP35 expression level was analyzed using Oncomine (p-value<1E-4; gene rank top 10%). Overall survival (OS) and disease-free survival (DFS) were evaluated by using GEPIA (median cutoff; HR displayed with 95% CI). STRING was used for analyzing the protein-protein interaction network, while WEBGESTALT for KEGG pathway and gene ontology (GO) of ARHGAP35 and associated proteins and cBioPortal for gene mutation. ARHGAP35 was overexpressed in several types of breast cancer, namely invasive ductal breast carcinoma (IDC), invasive ductal and lobular breast carcinoma (IDLC), invasive lobular breast carcinoma (ILC), male breast carcinoma, and mixed ductal and lobular carcinoma (MDLC). High expression of ARHGAP35 had significantly lower OS (p=0.045) compared to low expression of ARHGAP35 and the difference in DFS was not significant (p=0.98). ARHGAP35 interacted with RHOA, RHOB, RHOC, RHOD, RASA1, RND1, RAC1, CDC42, FYN and SRC. KEGG pathway and GO analysis showed that these proteins are highly involved in actin-based processes through adherent junction, axon guidance, focal adhesion, regulation of actin cytoskeleton, and tight junction. Mutation rate analysis showed 34 missense, 29 truncating, 3 fusion, and 1 in frame on ARHGAP35. Taken together, ARHGAP35 may involve in the growth and development of breast cancer through regulation of actin cytoskeleton pathway.Keywords: ARHGAP35, breast cancer, KEGG pathway, mutation rate, actin cytoskeleton.
Perbedaan Pengetahuan dan Sikap Masyarakat Kabupaten Banyumas tentang Diabetes Melitus Tipe 2 Sebelum dan Setelah Edukasi Parmasari, Damairia Hayu; Fareza, Muhammad Salman; Kusumawardani, Lita Heni; Hapsari, Pramesti Widya; Choironi, Nur Amalia; Aryani, Aisyah Apriliciciliana
Jurnal Ilmiah Kesehatan Masyarakat : Media Komunikasi Komunitas Kesehatan Masyarakat Vol 15 No 4 (2023): JIKM Vol. 15, Edisi 4, November 2023
Publisher : Public Health Undergraduate Program, Faculty of Health Science, Universitas Pembangunan Nasional Veteran Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52022/jikm.v15i4.475

Abstract

Latar Belakang: Diabetes melitus (DM) perlu dicegah karena tergolong penyakit silent killer. Sebagian kasus adalah DM Tipe 2. Berdasarkan hasil penelitian sebelumnya menunjukkan masyarakat di Banyumas sudah mempunyai tingkat pengetahuan cukup baik, tetapi belum mempunyai sikap yang cukup baik terhadap pencegahan DM Tipe 2. Perlu dilakukan edukasi kepada masyarakat serta deteksi dini DM melalui pemeriksaan gula darah. Tujuan penelitian ini untuk mengetahui perbedaan pengetahuan dan sikap masyarakat di Desa X, Kabupaten Banyumas terhadap DM Tipe 2 sebelum dan sesudah edukasi. Metode: Desain penelitian menggunakan quasi experiment dilakukan selama bulan Agustus 2023. Kuesioner digunakan untuk menilai pengetahuan dan sikap. Responden menjawab pertanyaan kuesioner pre-test, kemudian diberikan edukasi dan menjawab kuesioner post-test. Edukasi DM Tipe 2 dilakukan melalui penyampaian materi secara lisan dan tulisan melalui paparan menggunakan media slide Power Point dan modul. Sampel adalah masyarakat Desa X, Kabupaten Banyumas sebanyak 30 orang. Analisis perbedaan pengetahuan dan sikap menggunakan uji Wilcoxon.Hasil: Hasil uji Wilcoxon memperlihatkan bahwa tidak ada perbedaan signifikan pengetahuan dan sikap masyarakat Desa X tentang DM Tipe 2 sebelum dan sesudah edukasi dengan nilai p=0,07 dan p=0,948. Kesimpulan: Tidak ada perbedaan signifikan pengetahuan dan sikap masyarakat Desa X, Kabupaten Banyumas sebelum dan sesudah edukasi DM Tipe 2. Kata Kunci: Edukasi, Intervensi, Masyarakat, Pengetahuan, Sikap Introduction: Diabetes mellitus (DM) needs to be prevented because it is a silent killer disease. Some cases are Type 2 DM. Based on the results of previous research, it shows that the people in Banyumas already have a fairly good level of knowledge, but do not yet have a good enough attitude towards preventing Type 2 DM. Necessary to provide education to the community and early detection of DM through blood sugar checks. The study aimed to determine the differences in knowledge and attitudes of Village X community, Banyumas Regency towards Type 2 DM before and after education. Method: The research design used a quasi-experiment during August 2023. A questionnaire was used to measured knowledge and attitude. Respondents answered the pre-test, then were given education and answered the post-test. Type 2 DM education was carried out through the delivery of material orally and in writing through presentations using PowerPoint slides and modules. The sample were 30 people from Village X, Banyumas Regency. Analysis of differences in knowledge and attitudes using the Wilcoxon test. Results: The Wilcoxon test results show that there is no significant difference in the knowledge and attitudes of the people of Village X regarding Type 2 DM before and after education with a p-value of 0.07 and 0.948.Conclusion: There were no significant difference in the knowledge and attitudes of the people of Village X, Banyumas Regency pre and post Type 2 DM education.Keywords: Education, Intervention, Society, Knowledge, Attitude
Synthesis and Cytotoxic Activity of Methoxylated Chalcones in Breast Cancer MCF-7 and Prostate Cancer DU-145 Cell Lines Fareza, Muhamad Salman; Samudra, Genta Hafied Naga; Asrada, Syahdan; Fischellya, Dafi; Wijaya, Triyadi Hendra; Choironi, Nur Amalia; Wasito, Hendri; Suhesti, Tuti Sri; Mustikaningtyas, Ika; Rehana, Rehana; Setiyabudi, Lulu; Sarmoko, Sarmoko
Molekul Vol 20 No 3 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.3.13612

Abstract

Chalcones, a class of naturally occurring compounds, exhibit a broad spectrum of biological activities, including anticancer properties. In this study, a series of methoxylated chalcones were synthesized via Claisen-Schmidt condensation and evaluated for cytotoxic activity against breast cancer MCF-7 and prostate cancer DU-145 cell lines. The synthetic route involved Claisen-Schmidt condensation, leading to various methoxy-substituted chalcone derivatives. The structures of the synthesized chalcones were confirmed through NMR and mass spectrometry. Cytotoxicity was assessed using the PrestoBlue assay, with 4-bromochalcone (compound 2) displaying the highest cytotoxic activity against MCF-7 cancer cell lines (IC50 = 26.99 µM). These results indicate that methoxylated chalcones hold promise as potential lead compounds for the development of new anticancer agents targeting breast and prostate cancer.
In Silico Approach of Flavonol in Hibiscus sabdariffa as Proteasome Inhibitors Targeting the Ubiquitin-Proteasome Pathway Choironi, Nur Amalia; Prameswari, Syakhsiyatunnisa Galuh; Baroroh, Hanif Nasiatul
Chimica et Natura Acta Vol 13, No 3 (2025)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v13.n3.63571

Abstract

Multiple myeloma is a blood cancer characterized by the abnormal proliferation of B cells that accumulate in the bone marrow. The proliferation of these cells depends on the role of the proteasome through the ubiquitin-proteasome pathway to degrade proteins that regulate the cell cycle, apoptosis, and stress response. The proteasome is the main target of proteasome inhibitors for the treatment of multiple myeloma. Flavonoid compounds in Hibiscus sabdariffa flowers, such as isoquercitrin, quercitrin, quercetagetin, isorhamnetin, and astragalin, have the potential to act as proteasome inhibitors. The insilico study aims to determine the potential of these compounds as proteasome inhibitors based on the results of molecular docking and molecular dynamics simulation. Molecular docking was performed using AutoDock Vina, PyMol, and BIOVIA Discovery Studio. The best compounds from the docking results were then tested for interaction stability through molecular dynamics using YASARA software. Quercetagetin, isoquercitrin, and isohamnetin, based on the results of molecular docking, have the lowest binding affinity of -6.0, -5.7, and -5.5 kcal/mol, respectively. The three compounds bind to the active site of the 20S proteasome, namely Thr1, Ala20, Thr21, and Ala49. The RMSD values in the molecular dynamics of quercetagetin (1.290 Å), isorhamnetin (1.839 Å), and carfilzomib (1.843 Å). The binding affinity of quercetagetin (-422.623 kJ/mol), isorhamnetin (-438.388 kJ/mol), and carfilzomib (664.956 kJ/mol). Molecular docking and molecular dynamics findings indicate that isorhamnetin binds to the amino acid residues Thr1 and Ala49, which are the active sites of the 20S proteasome.
Co-Authors Aisyah Apriliciciliana Aryani Andayana Puspitasari Aprilia, Vika Asrada, Syahdan Fadlan, Arif Fareza, Muhamad Salman Fareza, Muhammad Salman Febrian, Dicky Rizky Fischellya, Dafi Hanif Nasiatul Baroroh Hapsari, Pramesti Widya HENDRI WASITO Joko Setyono kusumawardani, lita heni Mustikaningtyas, Ika Parmasari, Damairia Hayu Prameswari, Syakhsiyatunnisa Galuh Rehana, Rehana Samudra, Genta Hafied Naga Sarmoko Sarmoko Setiyabudi, Lulu SOETOMO, SUNARTO Tatang Irianti Triyadi Hendra Wijaya Tuti Sri Suhesti