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All Journal Jurnal Kedokteran Gigi Universitas Padjadjaran Kesmas Indonesia: Jurnal Ilmiah Kesehatan Masyarakat Journal of Indonesian Dental Association Indonesian Journal of Dentistry Biomedika
A. Haris Budi Widodo
Jurusan Farmasi, Universitas Jenderal Soedirman, Purwokerto, Jawa Tengah, Indonesia
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Medicine & Pharmacology Public Health

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STUDI TENTANG POLA HIDUP TIDAK SEHAT DALAM RANGKA PENINGKATAN TARAF KESEHATAN MASYARAKAT DI DESA KARANGGINTUNG KECAMATAN SUMBANG KABUPATEN BANYUMAS TAHUN 2009 Widodo, A. Haris Budi; Kusnandar, Kusnandar
Kesmas Indonesia: Jurnal Ilmiah Kesehatan Masyarakat Vol 3 No 1 (2010): Jurnal Kesmas Indonesia
Publisher : Jurusan Kesehatan Masyarakat dan Fakultas Ilmu-Ilmu Kesehatan Universitas Jenderal Soedirman

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Abstract

ABSTRACT Government continue to try to improve level health of society through various developed program. Behavior of society represent one of the level determinant health of society. This Research aim to know various indisposed behavior which done by Countryside society of Karanggintung District Sumbang of Sub-Province of Banyumas. This type research is research of observasional with method survey and this research have the character of analytic descriptive, that is research elaborating and diging how a phenomenon happened. Population at this research is all citizen residing in or residing in Countryside of Karanggintung  District Sumbang of Sub-Province of Banyumas counted 1.080 family head occupying house counted 973. Because research location do not too wide of, hence all population made as research sampel. Thereby this research is research of population. Data collecting done by observation or perception at research location. From result of research concluded as indisposed behavior which still done by Countryside society of Karanggintung District Sumbang of Sub-Province of Banyumas, covering: (1) take a bath, cleaning clothes and clean appliances/ housewares of household in river, (2) defecating, throwing away garbage and throw away family waste to river, (3) still many under the way house and look after livestock close to house, even include livestock into house, and (4) still many lawn or location which do not maintained (to be growed by crop no advantage, bamboo trees, big trees don't maintained, etc.), less grave location maintained, dirty gangways and growed by many grass, and also dirty street shoulders because grass and garbage) Keyword: Indisposed life pattern, Public health. Kesmasindo Volume 3, Nomor 1, Januari 2010, hlm. 66-75        
KEWASPADAAN UNIVERSAL PELAYANAN KESEHATAN GIGI DI PUSKESMAS SE-KABUPATEN BANYUMAS Handayani, Fani Tuti; Widodo, A. Haris Budi
Kesmas Indonesia: Jurnal Ilmiah Kesehatan Masyarakat Vol 3 No 1 (2010): Jurnal Kesmas Indonesia
Publisher : Jurusan Kesehatan Masyarakat dan Fakultas Ilmu-Ilmu Kesehatan Universitas Jenderal Soedirman

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ABSTRACT In the past last years, universal precaution have been introduced by The Ministry of Health, Republic of Indonesia. The sosialization of  universal precaution were function as an efforts on blood and body fluids infection control. Dental treatment procedures may cause any blood and body fluids that enable become an infecting agent. This research purpose was to determine the application of universal precaution on dental treatment prosedures in the public health center (Puskesmas) in the regency of Banyumas as an efforts on blood and body fluids infection control. This is a descriptive survey research. Sample in this research were total of the population constitude 39 public health center in the regency of Banyumas. The method used to collect data was interview with questionnaire sheets. An univariate analysis was used to analyze all data by SPSS computer program version 16 for windows. The result of this research shows 25,6% of dental polyclinic at public health center in the regency of Banyumas were categorized  as good in management of the needle or sharp applience perfectly. 38,5 % of dental polyclinic at public health center in the regency of Banyumas decontaminate all health appliances. 59 % of dental polyclinic at public health center in the regency of Banyumas were categorized as good in hand washing to prevent the cross infection. The application of perfect protection for blood exposure was 0%. 87,2% of  dental polyclinic at public health center in the regency of Banyumas do waste disposal properly and safety. Key Words :   Universal precaution, dental health, public health center. Kesmasindo Volume 3, Nomor 1, Januari 2010, hlm. 47-55
Potensi larutan teh hijau celup sebagai alternatif Hank’s Balanced Salt Solution mempertahankan viabilitas sel ligamen periodontal gigi avulsi: studi in vitro Zahratuljannah, Reshaina Dewi Azizah; Widodo, A. Haris Budi; Triani, Maulina; Ichsyani, Meylida; Rochmawati, Mutia
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 36, No 2 (2024): Agustus 2024
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v36i2.55391

Abstract

ABSTRAKPendahuluan: Avulsi gigi terjadi ketika gigi terlepas sepenuhnya dari soket alveolar akibat trauma. Perawatan awal yang dilakukan pada gigi avulsi/replantasi, yaitu menanamkan kembali gigi ke dalam soket dengan segera. Prognosis kesuksesan replantasi gigi sangat bergantung pada viabilitas sel ligamen periodontal sehingga memerlukan media penyimpanan yang sesuai dan manajemen waktu yang tepat. Penelitian bertujuan menganalisis potensi larutan teh hijau celup sebagai alternatif penggunaan Hank’s Balanced Salt Solution (HBSS) pada viabilitas sel ligamen periodontal pada gigi. Metode: Penelitian dilakukan secara eksperimental laboratoris in vitro menggunakan sampel sel ligamen periodontal pada 32 gigi insisivus maksila tikus Wistar. Sampel dibagi menjadi 8 kelompok, yaitu kelompok yang direndam dalam larutan teh hijau celup dan kelompok kontrol positif pada HBSS selama 1, 3, 6, dan 24 jam. Metode analisis penghitungan persentase viabilitas sel ligamen periodontal dengan pewarnaan eksklusi trypan blue dibawah mikroskop dengan perbesaran 100x. Analisis statistik menggunakan one way ANOVA untuk membandingkan persentase antar waktu pada teh hijau celup dan independent t-test untuk membandingkan teh hijau celup dan HBSS pada setiap waktu. Hasil: Hasil one way ANOVA p>0,05 yang menandakan bahwa tidak terdapat perbedaan signifikan antar perendaman dalam larutan teh hijau celup. Hasil independent t-test pada jam ke 1 dan 3 p>0,05 yang menandakan tidak terdapat perbedaan signifikan dan pada jam ke 6 dan 24 p<0,05 menandakan adanya perbedaan signifikan antara perendaman dalam larutan teh hijau celup dan larutan kontrol positif HBSS. Simpulan: Larutan teh hijau celup dapat menjadi alternatif HBSS dalam mempertahankan viabilitas sel ligamen periodontal gigi avulsi.Potential of green tea bag solution as an alternative to Hank’s Balanced Salt Solution in maintaining periodontal ligament cell viability in Wistar rat avulsed teeth: in vitro study Introduction: Tooth avulsion occurs when the tooth is completely separated from the alveolar socket due to trauma. The initial treatment for an avulsed tooth is replantation, namely immediately implanting the tooth back into the socket. The prognosis for successful tooth replantation is highly dependent on the viability of periodontal ligament cells. Use of appropriate storage media and proper time management are critical to preserving periodontal ligament cells and the likelihood of successful replantation. This study aims to analyze potential of a green tea bag solution as an alternative to using Hank’s Balanced Salt Solution (HBSS) on periodontal ligament cell viability in avulsed teeth. Methods: The research was carried out experimentally in vitro using periodontal ligament cell samples from 32 maxillary incisors of Wistar rats. The samples were divided into 8 groups, namely the group soaked in green tea bag solution and the positive control group in HBSS for 1, 3, 6, and 24 hours. The analytical method is to calculate the percentage of periodontal ligament cell viability using trypan blue exclusion staining under a microscope with 100x magnification. Statistical analysis used one way ANOVA to compare percentages between times of bagged green tea and independent t-test to compare bagged green tea and HBSS at each time. Results: The results of one way ANOVA were p>0.05 which indicated that there was no significant difference between immersion in green tea bag solution. The results of the independent t-test at the 1st and 3rd hours were p>0.05 which indicated there was no significant difference and at the 6th and 24th hours p<0.05 which indicated there was a significant difference between immersion in the green tea bag solution and HBSS as the positive control solution. Conclusion: Green tea bag solution can be an alternative to HBSS in maintaining periodontal ligament cell viability in avulsed teeth.
EFFECT OF KECOMBRANG ETHANOL EXTRACT (ETLINGERA ELATIOR) ON FUSOBACTERIUM NUCLEATUM BIOFILM DEGRAGATION Djati, Fanni Kusuma; Ichsyani, Meylida; Widodo, A. Haris Budi; Dewi, Aisha Tiara
Biomedika Vol 17, No 1 (2025): Biomedika August 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v17i2.12008

Abstract

Fusobacterium nucleatum is a Gram-negative anaerobic bacterium that plays a crucial role in periodontal disease progression through biofilm formation. Kecombrang (Etlingera elatior) is a traditional medicinal plant containing bioactive compounds with antimicrobial properties. This study aimed to evaluate the effect of kecombrang extract on F. nucleatum biofilm degradation. This experimental study used ethanolic extracts from three parts of kecombrang (leaves, stems, and flowers) at concentrations of 1.56, 3.12, 6.25, 12.50, 25, and 50 mg/mL. F. nucleatum biofilm was formed and treated with various extract concentrations. Chlorhexidine 0.2% was used as positive control and DMSO 1% as negative control. Biofilm degradation percentage was measured using crystal violet assay at 490 nm wavelength. Data were analyzed using Two-Way ANOVA to evaluate the main effects of plant parts and extract concentrations, followed by Tukey’s HSD post-hoc test. All kecombrang extracts showed dose-dependent biofilm degradation activity. Flower extract demonstrated the highest efficacy with degradation percentages ranging from 36.37% to 86.22% at concentrations 1.56-25 mg/mL. Leaf extract showed degradation of 38.2—82.08%, while the stem extract achieved 34.00-80.22% degradation at the same concentration range. The MBEC50 values were 2.62 mg/mL for flower extract, 3.68 mg/mL for leaf extract, and 4.99 mg/mL for stem extract. Kecombrang extracts possess significant biofilm degradation activity against F. nucleatum, with flower extract showing the most promising results comparable to chlorhexidine positive control
IRON OVERLOAD, COMPROMISED SALIVARY FUNCTION, AND DENTAL CARIES RISK IN BETA-THALASSEMIA MAJOR: A CROSS-SECTIONAL Ramadhani, Amilia; Pramuditya, Hafizh Zufar; Nurcahyani, Putri Silvia; Taqwim, Ali; Basalamah, Muhammad; Widodo, A. Haris Budi; Rujito, Lantip
Indonesian Journal of Dentistry Vol 5, No 2 (2025): August 2025
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/ijd.v5i2.17807

Abstract

Background: β-thalassemia major patients exhibit higher dental caries prevalence due to systemic complications including iron overload from regular blood transfusions. This study aimed to assess dental caries status, salivary pH, and buffer capacity in β-thalassemia major patients and investigate correlations with ferritin blood concentration.Method: A cross-sectional analytical study was conducted on 24 β-thalassemia major patients aged 12-17 years. Salivary function was assessed by salivary pH and buffer capacity. Dental caries status was evaluated using the DMF-T index. Ferritin blood concentrations were correlated with salivary function and dental caries using Pearson analysis.Outcome: Mean salivary pH was 5.74±0.63 (acidic), buffer capacity was 3.98±0.21, DMF-T score was 6.17±1.97, and ferritin level was 5830.54±2823.91 ng/mL. Ferritin levels showed moderate negative correlations with salivary pH (r=-0.718, p<0.001) and buffer capacity (r=-0.737, p<0.001), and positive correlation with DMF-T scores (r=0.696, p<0.001). Strong negative correlations were found between DMF-T scores and salivary pH (r=-0.915, p<0.001) and buffer capacity (r=-0.913, p<0.001).Conclusion: Iron overload significantly compromises salivary parameters, creating an acidogenic oral environment that promotes dental caries development in β-thalassemia major patients, emphasizing the need for integrated oral health monitoring in thalassemia management protocols.
The Activities of Torch Ginger Flower (Etlingera elatior) Ethanol Extract on Degradation of Porphyromonas gingivalis Biofilm as Periodontal Pathogen Putri, Devi Anisya; Widodo, A. Haris Budi; Ichsyani, Meylida; Naufalin, Rifda; -, Oedjijono
Journal of Indonesian Dental Association Vol 6 No 1 (2023): April
Publisher : Indonesian Dental Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jida.v6i1.882

Abstract

Introduction: Porphyromonas gingivalis is bacteria that can form biofilms as the main cause of periodontitis. Mouthwash therapy in long term can cause mucositis and even oral cancer. Antibacterial potential of torch ginger flower (Etlingera elatior) can be developed as an alternative adjuvant therapy for periodontitis. Objective: Aims of this research was to determine the effect of torch ginger flower ethanol extract against degradation of P. gingivalis biofilm. Methods: This research used ethanolic extract of torch ginger flower with concentrations 1.56 mg/mL, 3.125 mg/mL, 6.25 mg/mL, 12.5 mg/mL, 25 mg/mL, and 50 mg/mL. Chlorhexidine gluconate 0.2% was used as positive control and DMSO 1% was used as negative control. Measurement of P. gingivalis biofilm degradation used microtiter plate assay with crystal violet 1% staining which reads its optical density at wavelength of 450 nm. Data were analyzed by one way ANOVA and Post hoc LSD. Results: The percentage of P. gingivalis biofilm degradation with torch ginger flower ethanol extract sequentially were 12.47%, 30.56%, 57.12%, 71.36%, and 74.83%. The analysis showed that there was a significant difference (p<0,05) between treatment groups torch ginger flower ethanol extract, as well as between torch ginger flower ethanol extract with DMSO 1% and chlorhexidine gluconate 0.2%. Optimum concentration of ethanol extract of torch ginger flower on P. gingivalis biofilm degradation was 25 mg/mL and showed no significant difference with chlorhexidine gluconate 0,2% (p>0,05). Conclusion: Conclusion of this research is torch ginger flower (Etlingera elatior) ethanol extract has P. gingivalis biofilm degradation activity.biofilm
Co-Authors -, Oedjijono . Harwoko Ali Taqwim Amilia Ramadhani, Amilia Basalamah, Muhammad Dewi, Aisha Tiara Fani Tuti Handayani, Fani Tuti Fanni Kusuma Djati Farah Amiria, Farah Ichsyani, Meylida kusnandar kusnandar Lantip Rujito Mutia Rochmawati, Mutia Nurcahyani, Putri Silvia Pramuditya, Hafizh Zufar Putri, Devi Anisya Rifda Naufalin Triani, Maulina Zahratuljannah, Reshaina Dewi Azizah