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All Journal HAYATI Journal of Biosciences Bioma : Berkala Ilmiah Biologi Indonesian Journal of Biotechnology Berkala Bioteknologi Makara Journal of Science Jurnal Bioteknologi & Biosains Indonesia
IS HELIANTI
Center for Bioindustrial Technology, Laboratorium of Bioindustrial Technology, LAPTIAB BPPT Puspiptek -Serpong
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Immunology & microbiology Materials Science & Nanotechnology

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INCREASING RECOMBINANT PENICILLIN G ACYLASE PRODUCTION: GENETIC, PROTEIN ENGINEERING, AND PRODUCTIVITY IMPROVEMENT Helianti, Is
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1775

Abstract

B-lactam derived antibiotics are the most used globally for treatment against different infections caused by pathogenic bacteria and comprises 65% of the world antibiotics. Recently, penicillin G acylase (PGA) is used as biocatalyst for those B-lactam antibiotics production by which 6-aminopenicillanic acid (6-APA) or 7-aminodeacetoxycephalosporanic acid (7-ADCA) as the building blocks is produced. Commercialized PGA from native microbial resources are still limited to E. coli. Therefore, genetic engineering approach such as cloning and expression in other microbial hosts were assessed to enhance bacterial strains that produce PGA. However, such improvement could increase immature precursors accumulation and lowering the enzyme yield, activity, or stability. This review focus on the review of PGA recombinant produced by several microbial host, their expression levels, and improvement achieved by some modification such as replacement of signal peptide and promoter continued to protein engineering to utilize the enzymes in synthetizing amoxicillin rather than to hydrolyses Penicillin G.
Co-Authors Abinawanto Abinawanto Achnafani, Dini AGUSTIN KRISNA WARDANI Ahmad Wibisana, Ahmad Ajrullah, Mauludy Jutta Amarila Malik Anto Budiharjo Dini, Muthia Rahmah Fatiningtyas, Fairuz Andini Feronika Heppy Sriherfyna Furgeva, Natasha Gunawan, Ellen Fenix Hermin Pancasakti Kusumaningrum I Gede Eka Perdana Putra, I Gede Eka Perdana K, Hermin Pancasakti KHOMAINI HASAN Maria Ulfah Masdalifah, Masdalifah Mochamad Nurcholis Mulyawati, Lina Napitupulu, Riswanto Nataniel, Jocelyn NIKNIK NURHAYATI Pramesti, Nadya Shafa Prawiro, Sumarno Reto Rejeki Siti Ferniah Sabbathini, Gabriela Christy Safirah, Dearesty Tri Yudani MR Wulandari, Sri Rezeki