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All Journal Majalah Kedokteran Bandung Althea Medical Journal Jurnal Farmasi Klinik Indonesia Journal of Medicine and Health Indonesian Journal of Obstetrics & Gynecology Science Majalah Patologi Indonesia
Bethy S. Hernowo
Departement of Anatomical Pathology, Faculty of Medicine, Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital Bandung
Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Ekspresi Bcl-2 dan Caspase-3 Pascapaparan Hipoksia Hipobarik Intermiten Hidayat, Achmad; Wiradisastra, Kahdar; Hernowo, Bethy S.; Achmad, Tri Hanggono
Majalah Kedokteran Bandung Vol 43, No 4
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Hipoksia hipobarik intermiten sering dialami oleh awak pesawat, karena selama di dalam kabin pesawat bernapas dengan tekanan udara yang lebih rendah. Tubuh akan beradaptasi dengan cara mengikat oksigen lebih banyak dan juga mengurangi dampak hipoksia. Fungsi mitokondria akan terganggu pada hipoksia, yaitu permiabilitas membran luar mitokondria karena protein Bcl-2 menurun. Jika hipoksia berlanjut akan terjadi kebocoran membran mitokondria, pelepasan sitokrom-c, dan proses apoptosis berlangsung. Penelitian ini bertujuan menganalisis protein Bcl-2 sebagai antiapoptosis dan caspase-3 sebagai indikator apoptosis akibat paparan hipoksia hipobarik intermiten. Dilakukan penelitian eksperimental pada tikus jantan Spraque Dawley periode Januari–April 2010 dengan melakukan paparan hipoksia hipobarik intermiten satu sampai empat kali dengan interval satu minggu. Jantung tikus dijadikan spesimen untuk dilakukan pemeriksaan ekspresi protein dengan pulasan imunohistokimia di Departemen Patologi Anatomi RS Dr. Hasan Sadikin Bandung dan western blot di Bagian Biomolekuler FK Universitas Indonesia Jakarta. Ekspresi protein Bcl-2 meningkat sesuai dengan frekuensi paparan hipoksia hipobarik intermiten, sebaliknya ekspresi protein caspase-3 menurun (rs=-0,448, p=0,013). Dari penelitian ini dapat disimpulkan bahwa terjadi penurunan tingkat apoptosis akibat paparan hipoksia hipobarik intermiten, hal ini disebabkan mekanisme adaptasi natural yang ditandai dengan menurunnya apoptosis sel dan secara tidak langsung akan memberi efek kardioprotektif. [MKB. 2011;43(4):166–70].Kata kunci: Apoptosis, Bcl-2, caspase-3, hipoksia hipobarik intermitenBcl-2 and Caspase-3 Expression Post Exposure of Intermittent Hypobaric HypoxiaIntermittent hypobaric hypoxia often suffered by cabin crew due to the fact that they are breathing lower pressured air inside the plane cabin. Human body will adapt by binding more oxygen and reducing hypoxia effect. Mitochondria function will be irritated by hypoxia which affect, outer mithochondrial membrane permeability due to decrease of Bcl-2 protein. Later on if hypoxia continues mitochondrial membrane will leaked cytocrome-c will released and apoptotic pathway will occur. The purpose of this study was to analyze Bcl-2 protein as antiapoptosis and caspase-3 as apoptosis indicator of intermittent hypobaric hypoxia exposure. Experimental study >was subjected to Spraque Dawley male mice during January–April 2010 by exposing them to several intermittent hypobaric hypoxias (one to four treatment) in an interval of one week. Protein expression on mice heart cell were detected by immunohistochemistry in the Department of Pathology Anatomy Padjadjaran University-RS Dr. Hasan Sadikin Bandung and western blot methods in Department Biomolecullar Indonesia University Jakarta. Bcl-2 protein expressions increased according with the frequency of intermittent hypobaric hypoxia exposures while a reverse trend was found for caspase-3 protein expressions (rs=-0.448, p=0.013). From the study it can be concluded that apoptosis will be decreased as a result of intermittent hypobaric hypoxia exposures, which occurred from natural adaptation mechanism indicated by decrease of cell apoptosis and cardio protective effect will be emerged. [MKB. 2011;43(4):166–70].Key words: Apoptosis, Bcl-2, caspase-3, intermittent hypobaric hypoxia DOI: http://dx.doi.org/10.15395/mkb.v43n4.64
Gambaran Klinikopatologi Limfoma Sel B Besar Difus Tidak Tertentu di Rumah Sakit Umum Pusat Dr. Hasan Sadikin Bandung Tahun 2018-2023 Afiati; Hernowo, Bethy S.; Aminah, Hermin; Oehadian, Amaylia
Majalah Patologi Indonesia Vol. 34 No. 3, September 2025
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v34i3.675

Abstract

Introduction Diffuse large B-cell lymphoma, not otherwise specified (DLBCL, NOS), is the most common group of non-Hodgkin malignant lymphoma globally, representing 25%-40% of adult lymphoma cases. According to the cell of origin(COO), DLBCL NOS is classified into DLBCL germinal center B-cell like(GCB) and DLBCL non-germinal center B-cell like(non-GCB). Since the COO affects the prognosis of DLBCL NOS, this examination is important. Hans algorithm is the most frequently used to distinguish the GCB from non-GCB. This study aims to describe clinicopathological characteristics of DLBCL NOS at Dr. Hasan Sadikin General Hospital Bandung, 2018-2023. Methods The subjects of this retrospective descriptive study were DLBCL GCB and non-GCB patients based on Hans algorithm by IHC examination of CD10, BCL6, and MUM1 who received R-CHOP therapy at Dr. Hasan Sadikin General Hospital from 2018 to 2023. All data contained age, gender, B-symptoms, primary tumor location, stage, total International Prognostic Index (IPI) score, and immunochemotherapy status.  Results A total of 55 patients diagnosed with DLBCL NOS were collected in this study. 50 patients(90.9%) were classified as DLBCL non-GCB and 5 patients(9.1%) were classified as DLBCL GCB. The average age was 62 years, predominantly males(52.7%), extranodal disease(54.5%), no B symptoms(76.4%), and early stage(83.7%). 52 patients(94.6%) had a total IPI score of 0-1, 3 patients(5.4%) had a total IPI score of 2. 21 patients(38.2%) had a response, 13 patients(23.6%) had non-response, and 21 patients(38.2%) are still ongoing to R-CHOP therapy. Conclusion DLBCL NOS at Dr. Hasan Sadikin General Hospital from 2018-2023 mainly occurred in men with an average 62 years old and extranodal disease without B-symptoms. DLBCL non-GCB was predominant than GCB. Both DLBCL Non-GCB and GCB were mostly diagnosed at early stage, IPI low-risk group, and had response status to R-CHOP therapy similar to those are still ongoing to R-CHOP therapy.
Relationship Between Histopathology Grading (Nottingham System) and Expression of HER2/Subtype Luminal B in Breast Carcinoma with Lymph Node Metastases Irmaya, Dita; Hernowo, Bethy S.; Dewayani, Birgitta M.
Majalah Patologi Indonesia Vol. 34 No. 3, September 2025
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v34i3.650

Abstract

Background Molecular subtypes of breast carcinoma, histological grade, and lymph node msetastases are significant in determining the therapy and prognosis of the patient. The luminal B subtype comprises 15%-20% of breast cancers and has a more aggressive phenotype, higher histological grade, proliferative index, and a worse prognosis. This study aimed to evaluate the relationship between histopathological grading and expression of  HER2 with lymph node metastases in luminal B subtype breast carcinoma. Methods We analyzed 279 invasive breast carcinoma luminal-B subtypes from the anatomic pathology laboratory of Dr. Hasan Sadikin Bandung between January 2017 and March 2023. Histological grade using the Nottingham system, expression of HER2 using immunohistochemistry examination, and lymph node metastases status were obtained from anatomic pathology records. The association between histological grade and expression of HER2 with lymph node metastases was examined with chi-square tests. Result: In the current study, we included 279 subjects, dominated by patients whose age at diagnosis was less than 50 years old. The Chi-square analysis showed no statistically significant difference in tumor size between patients with metastasis and those without metastasis,P = 0.74. The Chi-square analysis showed a significant relationship between histopathological grade and lymph node metastases, p < 0.01 and expression HER2 status and lymph node metastasis  p < 0.05 Conclusion This study found that patients present with larger tumors classified as T3 and moderate to poor grade III and grade II histological grades. The statistical analysis showed no significant difference in the age of diagnosis between patients with metastasis and those without. However, the Chi-square analysis revealed a significant correlation between the histopathological grade and HER2 expression with lymph node metastasis.
Uncommon Metastatic Spread of Endometrial Carcinoma to the Posterior Thoracic Wall and Genital Tract in a Young Adult: A Case Report Mawardinata, Phindo; Suardi, Dodi; Harsono, Ali Budi; Homenta, Christian; Sinaga, Ferry Iskandar Kharisma; Susilo, Sulaeman Andrianto; Hernowo, Bethy S.; Harahap, Reza Rinaldy; Lubis, Munawar Adhar
Indonesian Journal of Obstetrics & Gynecology Science Volume 9 Number 1 March 2026
Publisher : Dep/SMF Obstetri & Ginekologi Fakultas Kedokteran Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/obgynia.v9i1.984

Abstract

Introduction: Endometrial carcinoma is a common gynecologic malignancy that predominantly affects postmenopausal women. Approximately 15% of cases occur in premenopausal patients, and more than 1% are diagnosed in women younger than 40 years. Distant metastases are uncommon and typically involve lymph nodes, lungs, or liver, whereas cutaneous and genital metastases are exceptionally rare. The micropapillary growth pattern, initially described in breast carcinoma, has also been identified in various organs and is associated with aggressive biological behavior, including frequent lymphovascular invasion and widespread dissemination. Case Report: A case of a 24-year-old woman with a prior history of micropapillary carcinoma excised from the posterior thoracic wall is reported. The patient presented with palpable vulvar and cervical masses. Pelvic MRI and PET-CT revealed stage IV C endometrial carcinoma with extensive dissemination involving the cervix, vulva, vagina, peritoneum, thoracic wall, and pleural cavity. Histopathological and immunohistochemical analyses confirmed endometrioid carcinoma with micropapillary features. Conclusion: This study demonstrates that the rare presentation of micropapillary endometrial carcinoma in a young patient highlights the importance of comprehensive diagnostic evaluation when unusual metastatic sites are encountered, to guide treatment and multidisciplinary management. Abstrak Pendahuluan: Karsinoma endometrium merupakan keganasan ginekologi yang umum dan terutama mengenai wanita pascamenopause. Sekitar 15% kasus terjadi pada pasien pramenopause dan lebih dari 1% pada wanita berusia di bawah 40 tahun. Metastasis jauh jarang terjadi dan biasanya melibatkan kelenjar getah bening, paru, atau hati, sedangkan metastasis ke kulit dan traktus genital sangat jarang dilaporkan. Pola pertumbuhan mikropapiler, yang awalnya dideskripsikan pada karsinoma payudara, juga ditemukan pada berbagai organ dan dikaitkan dengan perilaku biologis agresif, termasuk invasi limfovaskular dan penyebaran luas. Laporan Kasus: Dilaporkan kasus wanita berusia 24 tahun dengan riwayat karsinoma mikropapiler yang sebelumnya dieksisi dari dinding toraks posterior, datang dengan massa vulva dan serviks yang teraba. MRI pelvis dan PET-CT menunjukkan karsinoma endometrium stadium IV C dengan penyebaran luas ke serviks, vulva, vagina, peritoneum, dinding toraks, dan rongga pleura. Pemeriksaan histopatologi dan imunohistokimia menegakkan diagnosis karsinoma endometrioid dengan gambaran mikropapiler.Kesimpulan: Penelitian ini menunjukkan bahwa presentasi langka karsinoma endometrium dengan pola mikropapiler pada pasien usia muda menekankan pentingnya evaluasi diagnostik komprehensif pada lokasi metastasis tidak lazim untuk menentukan terapi dan tata laksana multidisiplin. 
Co-Authors - Afiati, - . Afiati, . Abdul H. Hassan, Abdul H. Abdul Hadi Hassan, Abdul Hadi Achmad Hidayat Afiati Afiati Afiati Agung Dinasti Permana Amaylia Oehadian Aminah, Hermin Aminah, Hermin Ani Melani Maskoen Anna Tjandrawati Beby S. Damayanti, Beby S. Birgitta M. Dewayani, Birgitta M. Dharmayanti Francisca Badudu, Dharmayanti Francisca Dimyati Achmad Dodi Suardi Een Hendarsih Erida, Yulie Fenny Ariyanni, Fenny Frans Ferdinal HANS-JOACHIM FREISLEBEN Harahap, Reza Rinaldy Harsono, Ali Budi Hasrayati Agustina Herry Yulianti Homenta, Christian Ida Parwati Iman Supandiman Indah Puti Rahmayani S. Irmaya, Dita Jeremy Sebastian Kahdar Wiradisastra Kiki A. Rizki Kiki Lukman Laely Yuniasari Lubis, Munawar Adhar Mawardinata, Phindo Mike Rezeki Mohamad Sadikin Nadytia Kusumadjayanti, Nadytia Nita Novita Nurwenda, Sartika Rachmat Sumantri Ris Kristiana, Ris Rostika Flora Septelia I. Wanandi Sinaga, Ferry Iskandar Kharisma Susilo, Sulaeman Andrianto Tri Hanggono Achmad Usman, Hermin A. Westiningrum, Retno Yenni Wisudarma, Yenni Yuniserani, Debbi