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All Journal Majalah Kedokteran Bandung Althea Medical Journal Jurnal Farmasi Klinik Indonesia Journal of Medicine and Health Majalah Patologi Indonesia
Bethy S. Hernowo
Departement of Anatomical Pathology, Faculty of Medicine, Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital Bandung
Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Ekspresi Bcl-2 dan Caspase-3 Pascapaparan Hipoksia Hipobarik Intermiten Hidayat, Achmad; Wiradisastra, Kahdar; Hernowo, Bethy S.; Achmad, Tri Hanggono
Majalah Kedokteran Bandung Vol 43, No 4
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Hipoksia hipobarik intermiten sering dialami oleh awak pesawat, karena selama di dalam kabin pesawat bernapas dengan tekanan udara yang lebih rendah. Tubuh akan beradaptasi dengan cara mengikat oksigen lebih banyak dan juga mengurangi dampak hipoksia. Fungsi mitokondria akan terganggu pada hipoksia, yaitu permiabilitas membran luar mitokondria karena protein Bcl-2 menurun. Jika hipoksia berlanjut akan terjadi kebocoran membran mitokondria, pelepasan sitokrom-c, dan proses apoptosis berlangsung. Penelitian ini bertujuan menganalisis protein Bcl-2 sebagai antiapoptosis dan caspase-3 sebagai indikator apoptosis akibat paparan hipoksia hipobarik intermiten. Dilakukan penelitian eksperimental pada tikus jantan Spraque Dawley periode Januari–April 2010 dengan melakukan paparan hipoksia hipobarik intermiten satu sampai empat kali dengan interval satu minggu. Jantung tikus dijadikan spesimen untuk dilakukan pemeriksaan ekspresi protein dengan pulasan imunohistokimia di Departemen Patologi Anatomi RS Dr. Hasan Sadikin Bandung dan western blot di Bagian Biomolekuler FK Universitas Indonesia Jakarta. Ekspresi protein Bcl-2 meningkat sesuai dengan frekuensi paparan hipoksia hipobarik intermiten, sebaliknya ekspresi protein caspase-3 menurun (rs=-0,448, p=0,013). Dari penelitian ini dapat disimpulkan bahwa terjadi penurunan tingkat apoptosis akibat paparan hipoksia hipobarik intermiten, hal ini disebabkan mekanisme adaptasi natural yang ditandai dengan menurunnya apoptosis sel dan secara tidak langsung akan memberi efek kardioprotektif. [MKB. 2011;43(4):166–70].Kata kunci: Apoptosis, Bcl-2, caspase-3, hipoksia hipobarik intermitenBcl-2 and Caspase-3 Expression Post Exposure of Intermittent Hypobaric HypoxiaIntermittent hypobaric hypoxia often suffered by cabin crew due to the fact that they are breathing lower pressured air inside the plane cabin. Human body will adapt by binding more oxygen and reducing hypoxia effect. Mitochondria function will be irritated by hypoxia which affect, outer mithochondrial membrane permeability due to decrease of Bcl-2 protein. Later on if hypoxia continues mitochondrial membrane will leaked cytocrome-c will released and apoptotic pathway will occur. The purpose of this study was to analyze Bcl-2 protein as antiapoptosis and caspase-3 as apoptosis indicator of intermittent hypobaric hypoxia exposure. Experimental study >was subjected to Spraque Dawley male mice during January–April 2010 by exposing them to several intermittent hypobaric hypoxias (one to four treatment) in an interval of one week. Protein expression on mice heart cell were detected by immunohistochemistry in the Department of Pathology Anatomy Padjadjaran University-RS Dr. Hasan Sadikin Bandung and western blot methods in Department Biomolecullar Indonesia University Jakarta. Bcl-2 protein expressions increased according with the frequency of intermittent hypobaric hypoxia exposures while a reverse trend was found for caspase-3 protein expressions (rs=-0.448, p=0.013). From the study it can be concluded that apoptosis will be decreased as a result of intermittent hypobaric hypoxia exposures, which occurred from natural adaptation mechanism indicated by decrease of cell apoptosis and cardio protective effect will be emerged. [MKB. 2011;43(4):166–70].Key words: Apoptosis, Bcl-2, caspase-3, intermittent hypobaric hypoxia DOI: http://dx.doi.org/10.15395/mkb.v43n4.64
Gambaran Klinikopatologi Limfoma Sel B Besar Difus Tidak Tertentu di Rumah Sakit Umum Pusat Dr. Hasan Sadikin Bandung Tahun 2018-2023 Afiati; Hernowo, Bethy S.; Aminah, Hermin; Oehadian, Amaylia
Majalah Patologi Indonesia Vol. 34 No. 3 (2025): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v34i3.675

Abstract

Introduction Diffuse large B-cell lymphoma, not otherwise specified (DLBCL, NOS), is the most common group of non-Hodgkin malignant lymphoma globally, representing 25%-40% of adult lymphoma cases. According to the cell of origin(COO), DLBCL NOS is classified into DLBCL germinal center B-cell like(GCB) and DLBCL non-germinal center B-cell like(non-GCB). Since the COO affects the prognosis of DLBCL NOS, this examination is important. Hans algorithm is the most frequently used to distinguish the GCB from non-GCB. This study aims to describe clinicopathological characteristics of DLBCL NOS at Dr. Hasan Sadikin General Hospital Bandung, 2018-2023. Methods The subjects of this retrospective descriptive study were DLBCL GCB and non-GCB patients based on Hans algorithm by IHC examination of CD10, BCL6, and MUM1 who received R-CHOP therapy at Dr. Hasan Sadikin General Hospital from 2018 to 2023. All data contained age, gender, B-symptoms, primary tumor location, stage, total International Prognostic Index (IPI) score, and immunochemotherapy status.  Results A total of 55 patients diagnosed with DLBCL NOS were collected in this study. 50 patients(90.9%) were classified as DLBCL non-GCB and 5 patients(9.1%) were classified as DLBCL GCB. The average age was 62 years, predominantly males(52.7%), extranodal disease(54.5%), no B symptoms(76.4%), and early stage(83.7%). 52 patients(94.6%) had a total IPI score of 0-1, 3 patients(5.4%) had a total IPI score of 2. 21 patients(38.2%) had a response, 13 patients(23.6%) had non-response, and 21 patients(38.2%) are still ongoing to R-CHOP therapy. Conclusion DLBCL NOS at Dr. Hasan Sadikin General Hospital from 2018-2023 mainly occurred in men with an average 62 years old and extranodal disease without B-symptoms. DLBCL non-GCB was predominant than GCB. Both DLBCL Non-GCB and GCB were mostly diagnosed at early stage, IPI low-risk group, and had response status to R-CHOP therapy similar to those are still ongoing to R-CHOP therapy.
Co-Authors - Afiati, - . Afiati, . Abdul H. Hassan, Abdul H. Abdul Hadi Hassan, Abdul Hadi Achmad Hidayat Afiati Afiati Afiati Agung Dinasti Permana Amaylia Oehadian Aminah, Hermin Aminah, Hermin Ani Melani Maskoen Anna Tjandrawati Beby S. Damayanti, Beby S. Birgitta M. Dewayani, Birgitta M. Dharmayanti Francisca Badudu, Dharmayanti Francisca Dimyati Achmad Een Hendarsih Erida, Yulie Fenny Ariyanni, Fenny Frans Ferdinal HANS-JOACHIM FREISLEBEN Hasrayati Agustina Herry Yulianti Ida Parwati Iman Supandiman Indah Puti Rahmayani S. Jeremy Sebastian Kahdar Wiradisastra Kiki A. Rizki Kiki Lukman Laely Yuniasari Mike Rezeki Mohamad Sadikin Nadytia Kusumadjayanti, Nadytia Nita Novita Nurwenda, Sartika Rachmat Sumantri Ris Kristiana, Ris Rostika Flora Septelia I. Wanandi Tri Hanggono Achmad Usman, Hermin A. Westiningrum, Retno Yenni Wisudarma, Yenni Yuniserani, Debbi