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All Journal Jurnal Kedokteran Brawijaya The Journal of Experimental Life Sciences (JELS) Mutiara Medika: Jurnal Kedokteran dan Kesehatan MNJ (Malang Neurology Journal) Medical Laboratory Technology Journal Jurnal Ilmiah Farmako Bahari Jurnal Kebidanan Akademi Kebidanan Jember (JKAKJ) Indonesian Archives of Biomedical Research (InABR) Journal of Fisheries & Marine
Dwi Yuni Nur Hidayati
Program Studi Biomedik, Fakultas Kedokteran, Universitas Brawijaya, Malang
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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Journal : Journal of Fisheries

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Pengaruh Induksi Bakteri Pseudomonas aeruginosa Terhadap Human Umbilical Vein Endothelial Cells (HUVECS) Culture [ The Influence Of Pseudomonas aeruginosa Induction To The Human Umbilical Vein Endothelial Cells (HUVECS) Culture ] Dwi Yuni Nur Hidayati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 1 No. 1 (2009): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v1i1.11691

Abstract

Abstract One of the cause of negative gram bacteria is Pseudomonas aeruginosa (Ps. aeruginosa). It continuing become sepsis on the first step of patogenesa happen sticking between aeruginosa with endhotel cell. This sticking is mediated by adhesi molecul which have characterize same with hemaglutin protein. The main study of this research is to know the profile of endothelial cell culture within induction of Ps. aeruginosa. The methods are Ps. aeruginosa (9064) which have been isolated then processes on TCBS Media and continued with isolation terracely. The profile of weight molecule protein obtained by SDS PAGE and then conducted electroelution. The test of hemaglutinin using eritrosit mencit. The receptor cell used is endhotel cell (HUVECs) culture. The result show that dosage of hemaglutin protein of Aeruginosa bring effect to profile of HUVECs culture determined by index adhesion of Ps. aeruginosa to HUVECs culture. We conclude that protein 38,19 kDa give effect to profile of HUVECs culture in vitro.
Co-Authors Agustina Tri Endharti Andre William Tulle Aris Widayati Azalia Ayu Rysaputri Elisa Danik Kurniawati Eviana Norahmawati Hartono, Kevin Hening Ryan Aryani Hery Susilo Hidayat Sujuti Irene Ratridewi Istifadatul Ilmiya Ni Putu Sri Haryati Noorhamdani A.S. Noorhamdani Noorhamdani Nuning Winaris Nur Rohmah Prihantanti Nur Rohmah Prihatanti Prasetyo Adi Rima Nor Kaspia, Rima Nor Roekistiningsih Roekistiningsih Roekistiningsih Roekistiningsih Santoso, Giovani Anggasta Soeyati Poejiani Sri Andarini Sri Murwani Tanti Tri Lestary Tatit Nurseta Wening Prastowo Wiyasa, I Wayan Arsana