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All Journal Jurnal Kedokteran Brawijaya The Journal of Experimental Life Sciences (JELS) Mutiara Medika: Jurnal Kedokteran dan Kesehatan MNJ (Malang Neurology Journal) Medical Laboratory Technology Journal Jurnal Ilmiah Farmako Bahari Jurnal Kebidanan Akademi Kebidanan Jember (JKAKJ) Indonesian Archives of Biomedical Research (InABR) Journal of Fisheries & Marine
Dwi Yuni Nur Hidayati
Program Studi Biomedik, Fakultas Kedokteran, Universitas Brawijaya, Malang
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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THE EFFECT OF INFECTION OF MYCOBACTERIUM TUBERCULOSIS STRAIN H37RV TOWARDS THE EXPRESSION OF TNF- α IN THE BRAIN Santoso, Giovani Anggasta; Sujuti, Hidayat; Hidayati, Dwi Yuni Nur
Malang Neurology Journal Vol 3, No 1 (2017): January
Publisher : Malang Neurology Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (580.751 KB) | DOI: 10.21776/ub.mnj.2017.003.01.3

Abstract

Background. Tuberculosis caused by mycobacterium tuberculosis. The support of these inflamated factors contained in this focus determine the prognosis of TB towards Central Nervous System. One of the inflammation factors is TNF-α.Objective. To find the expression of TNF-α on the brain which are infected by M. tuberculosis.Methods. Experimental study, Wild Mus musculus to compare the group which has got infected before. The similiar study has been conducted by Laksmi Wulandari, pulmonologist. The observation towards the expression of TNF-a into a mouse’s brain was done by using the imunohistokimia method. It is marked by the brown color in the core, sitoplasma and the cell with upgrading size 100x times 20.Results. This is evident that significanly corelation (P = 0.000) between independent Mycobacterium tuberculosis infection at incubation periode 8 and 16 weeks) and dependent variabel (TNF-α expression on brain winstar tissue that infected by Mycobacterium tuberculosis for 8 and 16 weeks).Conclusion. Strong relationship between expression of TNF-a and the M. Tuberculosis’ infection.
MYCOBACTERIUM TUBERCULOSIS STRAIN H37RV INFECTION TOWARDS MATRIX METALLOPROTEINASE (MMP)-2 IN BRAIN Hartono, Kevin; Adi, Prasetyo; Hidayati, Dwi Yuni Nur
Malang Neurology Journal Vol 3, No 2 (2017): July
Publisher : Malang Neurology Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (491.56 KB) | DOI: 10.21776/ub.mnj.2017.003.02.2

Abstract

Background. Tuberculous infection in brain can cause microglia to secrete inflammatory factors like Tumor Necrosis Factor alpha (TNF-α) and Interleukin 1 beta (IL-1β) which will be shown as body immune respons. Those inflammatory factors eventually can trigger microglia to secrete Matrix Metalloproteinase-2 (MMP-2) which will regenerate necrotic or apoptosis cells because of inflammation process. MMP-2 has been proven to have important role in brain tuberculous infection.Objective. To ascertain MMP-2 expression in mus musculus brain tissue with no infection, infection for 8 weeks, and infection for 16 weeks.Methods. This research used semiquantitative method to compare MMP-2 expression in 3 samples group. Observation of MMP-2 expression in mus musculus brain tissue were made by using immunohistochemistry colouration method which then would be observed in microscope with 400x magnification. Brain cell which express MMP-2 will become brown in cell nucleus, cytoplasm, and wall.Results. The result which had be obtained was overtime reduction of MMP-2 expression.Conclusion. MMP-2 expression didn’t decrease after 8 weeks time of infection.
THE INFECTION EFFECT OF STRAIN H37Rv MYCOBACTERIUM TUBERCULOSIS ON APOPTOSIS OF MICE’S NEURON CELL BRAIN (MUS MUSCULUS) Kaspia, Rima Nor; Hidayati, Dwi Yuni Nur; Widayati, Aris
Malang Neurology Journal Vol 2, No 2 (2016): July
Publisher : Malang Neurology Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (615.744 KB) | DOI: 10.21776/ub.mnj.2016.002.02.2

Abstract

Background. Ischemia on the cells and the lack of supply of glucose, will trigger a Ca2 + influx into the cell and increased expression of glutamate. Result in mitochondrial Ca2 + Influx be "overloaded". Glucose metabolism then switched to the anaerobic process that makes ATP increasingly depleted and there acidosis. This situation makes the neuron cell apoptosis triggered to occur. Apoptosis is programmed cell death. Neuron cell apoptosis is thought to have a strong connection to the tuberculosis infection in the brain.Objective. To determine the number of cells undergoing apoptosis neurons in brain tissue of mice.Methods. This study is a semiquantitative by comparing the number of cells undergoing apoptosis neurons in the three groups of samples. Observations apoptosis of neuronal cells in the brain tissue of mice was conducted using TUNEL staining technique (terminal deoxynucleotidyl transferase dUTP nick end labeling) are seen in a microscope with a magnification of 1000x.Results. The results showed that neuronal cells undergo apoptosis in brain tissue infected with M.tb for 8 and 16 weeks were marked with brown color in the cell nucleus. Neuron cell apoptosis were observed at M.tb-infected brain tissue for 16 weeks.Conclusion. The longer the M.tb infection can affect the increase in the number of neuronal cell apoptosis.
IDENTIIFKASI PROTEIN IMUNOGENIK CHLAMYDIA PNEUMONIAE TERHADAP SERUM PENDERITA INFARK MIOARD AKUT Murwani, Sri; Hidayati, Dwi Yuni Nur
Jurnal Kedokteran Brawijaya Vol 23, No 2 (2007)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (661.466 KB) | DOI: 10.21776/ub.jkb.2007.023.02.6

Abstract

Chlamydia pneumoniae is human respiratory tract pathogen and recently investigated as pathogen causingatherosclerosis and acute myocardial infarction (AMI). This research was carrier out to detect proteinpattern of C. pneumoniae, and to study it relation to AMI throughdetection of immunogenic protein. Design research  was laboratory observational and analyzed descriptively. The subject was C. pneumoniae. Protein pattern the bacteria was detected by electrophoresis method, and to detect the immunogenic protein was done immunoblotting. Serum was obtained from AMI patients in Saiful Anwar and Lavallette hospitals.  The result showed, protein pattern C. pneumoniae wasprotein with molecular weight 117, 107, 97, 91, 86, 61, 58, 52, 46, 44, 34, 23, 19, 9, 5, 4 kDa. Immunogenic proteins vary between AMI patients was 117, 107, 86, 61, 58, 52, 46, 44,  34 kDa. Non immunogenic proteins were 97, 91, 23, 19, 9, 5 and 4 kDa. Protein 61 kDa react to all of patient’s serum. It was concluded, C. pneumoniae have protein fractions 117, 107, 97, 91, 86, 61, 58, 52, 46, 44, 34, 23, 19, 9, 5, and 4kDa. Immunogenic proteins vary between AMI patients was 117, 107, 86, 61, 58, 52, 46, 44, 34 kDa, and 61 kDa was the  immunodominant protein. The result proved C. pneumoniae as causative agent of atherosclerosis and acute myocardial infarction, in Indonesia particularly. Key words:C. pneumoniae, immunogenic, AMI
Uji Kepekaan Mycobacterium sp. terhadap Isoniazid (INH) Menggunakan Metode Rasio Resistensi secara In Vitro Hidayati, Dwi Yuni Nur; Roekistiningsih, Roekistiningsih
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 13, No 3 (2013)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v13i3.2484

Abstract

Infeksi mikobakteria termasuk diantara infeksi yang paling sulit disembuhkan dari semua jenis infeksi bakteri. Mikobakteria yang patogen utamanya adalah Mycobacterium tuberculosis sebagai penyebab tuberkulosis (TB), yang mengakibatkan lebih banyak kematian daripada agen mikroba tunggal lainnya di seluruh dunia. Penelitian ini menggunakan Mycobacterium sp. yang bermanifestasi klinis tuberkulosis atau mirip tuberkulosis. Salah satu usaha untuk mengobati penyakit infeksi akibat Myco­bacterium sp. yang bermanifestasi klinis penyakit tuberkulosis atau mirip tuberkulosis adalah dengan menggunakan isoniazid. Terapi tunggal dengan isoniazid dan kegagalan penggunaan isoniazid ditambah obat lain yang sesuai telah mengakibatkan prevalensi resistensi isoniazid sebesar 10-20% dalam uji klinis di Karibia dan Asia Tenggara. Penelitian ini bertujuan untuk mengetahui apakah bakteri Mycobac­terium sp. yang berasal dari pasien RSSA Malang sudah resisten terhadap isoniazid. Penelitian bersifat semi kuantitatif untuk mengetahui resistensi Mycobacterium sp. terhadap isoniazid dengan menggunakan metode rasio resistensi. Dasar dari metode ini adalah membandingkan minimal inhibitory concentration (MIC) dari strain Mycobacterium sp. dengan MIC dari isoniazid untuk M. tuberculosis strain H37RV. Hasil penelitian menunjukkan bahwa 40% dari total isolat adalah resisten, 20% adalah intermediate resistant dan 40% adalah sensitif. Disimpulkan bahwa telah terjadi resistensi Mycobacterium sp. terhadap isoniazid.Mycobacteria’s infection is one of the most difficult cured infection from all over kind of bacteria’s infection. The major pathogen mycobacteria is Mycobacterium tuberculosis, agent of tuberculosis (TB) that causes more deaths than other single microbes in the world. This research uses Mycobacterium sp. which clinically manifest as tuberculosis or tuberculosis-like disease. One of efforts to cure this infection is by using isoniazid. Single dose of isoniazid and failed use of isoniazid plus other suitable drug have resulted prevalence of isoniazid-resistant about 10-20% in clinical test in Caribbean and South East Asia. This research is aimed to know whether Mycobacterium sp. from RSSA Malang patients has been resistant toward isoniazid. This research uses a semi quasi experimental research to know the resis­tance of Mycobacterium sp. toward isoniazid by using resistance rasio method. This method compares minimal inhibitory concentration (MIC) of Mycobacterium sp. strain with MIC from isoniazid to M. tuber­culosis strain H37RV. Result of this research indicates that 40% from total isolates are resistant, 20% are intermediate resistant, and 40% are sensitive. The conclusion is Mycobacterium sp. has been resistant toward isoniazid.
Cowpea (Vigna unguiculata) Extract Reduce Malondialdehyde Levels and Prevent Aortic Endothelial Cell Decline in Ovariectomized Rats Ni Putu Sri Haryati; Elisa Danik Kurniawati; Tanti Tri Lestary; Eviana Norahmawati; I Wayan Arsana Wiyasa; Dwi Yuni Nur Hidayati; Tatit Nurseta
Medical Laboratory Technology Journal Vol. 7 No. 2 (2021): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (484.221 KB) | DOI: 10.31964/mltj.v0i0.402

Abstract

Estrogen has anti-oxidative and anti-inflammatory properties, but its levels decrease in postmenopausal women who can trigger oxidative stress. One of the most damaging effects of ROS is lipid peroxidation, and the end product is Malondialdehyde (MDA). Similarly, aging endothelium has increased oxidative stress and endothelial cell sensitivity to apoptosis. This study aimed to determine the effect of cowpea extract on serum MDA levels, aortic endothelial cell counts, and brain MDA levels in the ovariectomy model. Cowpea extract can be used as an alternative to prevent and overcome the effects that occur during menopause, such as cardiovascular problems, decreased bone mineral density, and dementia. The study used 15-month-old female Rattus norvegicus, divided into six groups (OVX, SHAM, OVX+estradiol, OVX+Vu 1.25; 2.5; and 5 mg/kg BW/day). Serum and brain MDA levels were examined by ELISA method, while the number of aortic endothelial cells were examined on histopathological preparations with Hematoxylin & Eosin (HE) staining. The mean value of serum and brain MDA levels decreased with an increase in the dose given (p-value 0.016). The mean value of aortic endothelial cells between the dose groups did not significantly differ. However, the mean value showed an increasing trend as the dose of cowpea extract was given. The results of this study indicate that the extract of cowpea has the potential as an antioxidant to reduce serum and brain MDA levels, prevent a decrease in the number of aortic endothelial cells. As prevention, cowpea extract can be used as an antioxidant and consumed since premenopause to minimize problems that occur during postmenopause.
Uji Kepekaan Mycobacterium sp. terhadap Isoniazid (INH) Menggunakan Metode Rasio Resistensi secara In Vitro Dwi Yuni Nur Hidayati; Roekistiningsih Roekistiningsih
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 13, No 3 (2013)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v13i3.2484

Abstract

Infeksi mikobakteria termasuk diantara infeksi yang paling sulit disembuhkan dari semua jenis infeksi bakteri. Mikobakteria yang patogen utamanya adalah Mycobacterium tuberculosis sebagai penyebab tuberkulosis (TB), yang mengakibatkan lebih banyak kematian daripada agen mikroba tunggal lainnya di seluruh dunia. Penelitian ini menggunakan Mycobacterium sp. yang bermanifestasi klinis tuberkulosis atau mirip tuberkulosis. Salah satu usaha untuk mengobati penyakit infeksi akibat Myco­bacterium sp. yang bermanifestasi klinis penyakit tuberkulosis atau mirip tuberkulosis adalah dengan menggunakan isoniazid. Terapi tunggal dengan isoniazid dan kegagalan penggunaan isoniazid ditambah obat lain yang sesuai telah mengakibatkan prevalensi resistensi isoniazid sebesar 10-20% dalam uji klinis di Karibia dan Asia Tenggara. Penelitian ini bertujuan untuk mengetahui apakah bakteri Mycobac­terium sp. yang berasal dari pasien RSSA Malang sudah resisten terhadap isoniazid. Penelitian bersifat semi kuantitatif untuk mengetahui resistensi Mycobacterium sp. terhadap isoniazid dengan menggunakan metode rasio resistensi. Dasar dari metode ini adalah membandingkan minimal inhibitory concentration (MIC) dari strain Mycobacterium sp. dengan MIC dari isoniazid untuk M. tuberculosis strain H37RV. Hasil penelitian menunjukkan bahwa 40% dari total isolat adalah resisten, 20% adalah intermediate resistant dan 40% adalah sensitif. Disimpulkan bahwa telah terjadi resistensi Mycobacterium sp. terhadap isoniazid.Mycobacteria’s infection is one of the most difficult cured infection from all over kind of bacteria’s infection. The major pathogen mycobacteria is Mycobacterium tuberculosis, agent of tuberculosis (TB) that causes more deaths than other single microbes in the world. This research uses Mycobacterium sp. which clinically manifest as tuberculosis or tuberculosis-like disease. One of efforts to cure this infection is by using isoniazid. Single dose of isoniazid and failed use of isoniazid plus other suitable drug have resulted prevalence of isoniazid-resistant about 10-20% in clinical test in Caribbean and South East Asia. This research is aimed to know whether Mycobacterium sp. from RSSA Malang patients has been resistant toward isoniazid. This research uses a semi quasi experimental research to know the resis­tance of Mycobacterium sp. toward isoniazid by using resistance rasio method. This method compares minimal inhibitory concentration (MIC) of Mycobacterium sp. strain with MIC from isoniazid to M. tuber­culosis strain H37RV. Result of this research indicates that 40% from total isolates are resistant, 20% are intermediate resistant, and 40% are sensitive. The conclusion is Mycobacterium sp. has been resistant toward isoniazid.
PENGARUH PROTEIN PILI Streptococcus Agalactiae TERHADAP EKSPRESI β-Defensin-2 PADA TUBA FALLOPI TIKUS PUTIH (Rattus novergicus) GALUR WISTAR Nur Rohmah Prihatanti; Hening Ryan Aryani; Istifadatul Ilmiya; Noorhamdani A.S.; Dwi Yuni Nur Hidayati
Jurnal Ilmiah Farmako Bahari Vol 12, No 1 (2021): Jurnal Ilmiah Farmako Bahari
Publisher : Fakultas MIPA Universitas Garut

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52434/jfb.v12i1.1001

Abstract

Salah satu infeksi pada organ genitalia wanita adalah infeksi tuba falopi. Infeksi tuba fallopi dapat disebabkan oleh Streptococcus agalactiae. Pencegahan infeksi yang disebabkan oleh Streptococcus agalactiae dapat dilakukan dengan vaksinasi. Protein pili Streptococcus agalactiae merupakan kandidat vaksin yang baik. Pili Streptococcus agalactiae berperan penting dalam memfasilitasi ikatan antara Streptococcus agalactiae dengan sel inang. Protein pili Streptococcus agalactiae didapatkan dari proses pemotongan pili. Tikus putih (Rattus novergicus) galur wistar dikelompokkan menjadi 5 kelompok (kontrol dan perlakuan). Satu minggu setelah pemberian protein pili Streptococcus agalactiae terakhir, tikus putih (Rattus novergicus) galur wistar dikorbankan menggunakan teknik dekapitasi. Jaringan tuba falopi diambil dan dilakukan pemeriksaan imunohistokimia. Ekspresi β-defensin-2 hasil imunohistokimia diamati menggunakan mikroskop dengan perbesaran 400x dan dihitung menggunakan software Image-J. Terdapat perbedaan ekspresi β-defensin-2 yang signifikan pada tuba fallopi tikus putih (Rattus novergicus) galur wistar kelompok kontrol dan perlakuan. Semakin tinggi dosis protein pili Streptococcus agalactiae, semakin tinggi pula ekspresi β-defensin-2 pada tuba fallopi tikus putih (Rattus novergicus) galur wistar. Kata kunci: β-defensin-2, Tuba Fallopi, Protein Pili Streptococcus agalactiae
Detection of COVID-19 Cases in the Hospital of Universitas Brawijaya Malang Nuning Winaris; Andre William Tulle; Soeyati Poejiani; Sri Andarini; Wening Prastowo; Agustina Tri Endharti; Dwi Yuni Nur Hidayati
Indonesian Archives and Biomedical Sciences Vol 2 No 1 (2022): Indonesian Archives of Biomedical Research (InABR) 2(1): 2022
Publisher : Konsorsium Ilmu Biomedik Indonesia (KIBI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55392/indarcbiores.v2i1.29

Abstract

The global pandemic of COVID-19 was caused by a novel coronavirus identified as SARS-CoV-2. The outbreak started in Wuhan, China and spread rapidly all over the world. Escalation of daily test capacity can accelerate the new suspect of COVID-19 cases finding and rapidly trace the close contacts of confirmed cases. In this study, we reported the total number of confirmed cases based on the number of samples tested in the diagnostic laboratory of the Hospital of Universitas Brawijaya Malang. The data for this study were collected from the result of the RT-qPCR test, which was used as the gold standard to identify active cases of COVID-19. The data collection was started from May 2020 until April 2021. Based on our findings, it appeared that the peak of the first wave of COVID-19 happened between December 2020 and January 2021. Keywords: COVID-19; RT-qPCR; ‎‎‎‎‎‎‎‎‎‎‎‎SARS-CoV-2
Pengaruh Pemberian Protein Pili Streptococcus agalactiae pada Ketebalan Epitel Endometrium Rattus Norvegicus Hening Ryan Aryani; Istifadatul Ilmiya; Nur Rohmah Prihantanti; Dwi Yuni Nur Hidayati; Noorhamdani Noorhamdani
JURNAL KEBIDANAN AKADEMI KEBIDANAN JEMBER Vol 6, No 1 (2022): MARET
Publisher : Akademi Kebidanan Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Infeksi Endometrium merupakan peradangan pada lapisan dalam rahim yang disebabkan karena adanya infeksi yang salah satunya disebabkan oleh Streptococcus agalactiae. Streptococcus agalactiae merupakan bakteri Gram positif yang dapat ditemukan pada saluran genetalia dan gastrointestinal sekitar 30% pada wanita. Akan tetapi bakteri ini mampu beradaptasi sesuai dengan lingkungannya sehingga dapat berubah dari bakteri komensal menjadi pathogen. Penelitian ini merupakan penelitian eksperimental murni yang terdiri dari 25 ekor Rattus norvegicus berjenis kelamin perempuan dan  dibagi menjadi 5 kelompok dimana setiap tikus diberikan perlakuan pada hari ke 0, 7 dan 14. Pengambilan data dilakukan menggunakan software image raster 3 dan pengumpulan data kemudian di analisis menggunakan One Way Anova. Hasil penelitian menunjukkan rerata ketebalan epitel endometrium pada kelompok kontrol positif memiliki rerata terendah yaitu 17,89μm, sedangkan rerata ketebalan epitel endometrium tertinggi terdapat pada kelompok perlakuan ketiga yaitu 32,86 μm. Sedangkan hasil tes One Way Anova menunjukkan p=0,000 (p-value<0,05), yang membuktikan bahwa terdapat perbedaan peningkatan yang bermakna terhadap ketebalan epitel endometrium antara Rattus norvegicus pada masing-masing kelompok perlakuan. Maka dapat disimpulkan bahwa pemberian protein pili Streptococcus agalactiae terbukti meningkatkan ketebalan epitel endometrium Rattus norvegicus
Co-Authors Agustina Tri Endharti Andre William Tulle Aris Widayati Azalia Ayu Rysaputri Elisa Danik Kurniawati Eviana Norahmawati Hartono, Kevin Hening Ryan Aryani Hery Susilo Hidayat Sujuti Irene Ratridewi Istifadatul Ilmiya Ni Putu Sri Haryati Noorhamdani A.S. Noorhamdani Noorhamdani Nuning Winaris Nur Rohmah Prihantanti Nur Rohmah Prihatanti Prasetyo Adi Rima Nor Kaspia, Rima Nor Roekistiningsih Roekistiningsih Roekistiningsih Roekistiningsih Santoso, Giovani Anggasta Soeyati Poejiani Sri Andarini Sri Murwani Tanti Tri Lestary Tatit Nurseta Wening Prastowo Wiyasa, I Wayan Arsana