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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Veteriner Current Biochemistry Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Warta IHP (Warta Industri Hasil Pertanian) Pharmascience FITOFARMAKA: Jurnal Ilmiah Farmasi Indonesian Journal of Applied Research (IJAR) Borneo Journal of Pharmacy
Husnawati, .
Department Of Biochemistry, Faculty Of Mathematics And Natural Sciences, IPB University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Education Industrial & Manufacturing Engineering Medicine & Pharmacology Veterinary

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Hasil Uji Toksisitas Subkronis Temulawak Terhadap Nilai Hemoglobin, Hematokrit, dan Leukosit Tikus Akhmad Endang Zainal Hasan; Husnawati Husnawati; Siti Wachidatun Zulaikha
Jurnal Pharmascience Vol 9, No 2 (2022): Jurnal Pharmascience
Publisher : Program Studi Farmasi FMIPA Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v9i2.13374

Abstract

Herbal yang berperan sebagai antioksidan dan antikanker diantaranya temulawak. Penelitian pengaruh ekstrak temulawak terhadap hematologi tikus pada pengujian keamanan subkronis dilakukan pada penelitian ini.  Parameter hematologi yang ditentukan adalah nilai hemoglobin, hematokrit, dan jumlah leukosit. Hewan coba yang digunakan adalah tikus jantan dan betina dari galur Sprague Dawley. Herbal yang diberikan sebagai pengobatan adalah ekstrak etanol 70% temulawak yang diberikan sebanyak 5 hari dalam seminggu selama 28 hari dengan dosis bertingkat mulai dari 45 mg/Kg BB hingga 5.625 mg/kg BB. Hasil yang diperoleh adalah perlakuan terakhir tidak ada tikus yang mati. Berdasarkan pengukuran diperoleh nilai hemoglobin antara 12,57-17,97 g/dL, nilai hematokrit antara 31,77-51,57%, dan jumlah leukosit antara 5,01-23.22x103 sel/μL (normal). Kesimpulan dari penelitian ini adalah bahwa dosis lebih besar dari 5625 mg/kg BB merupakan nilai LD50 untuk ekstrak etanol 70% temulawak. Pemberian ekstrak etanol 70% temulawak hingga dosis 5.625 mg/kg BB selama 28 hari pada tikus jantan dan betina memberikan efek penurunan terhadap nilai hemoglobin, hematokrit, dan leukosit, namun nilainya masih dalam batas normal. Tidak ada perbedaan nyata antara tikus jantan dan betina pada hasil pengukuran nilai hemoglobin, hematokrit, dan leukosit. Kata Kunci: Dosis, Hematologi, Subkronis, Temulawak, Tikus  Herbs that act as antioxidants and anticancer include temulawak. Research on the effect of temulawak extract on rat hematology in subchronic safety testing was carried out in this study. The determined hematological parameters were the value of hemoglobin, hematocrit and the number of leukocytes. The experimental animals used were male and female rats from the Sprague Dawley strain. The herbs given as treatment were an ethanolic extract of 70% temulawak which was given 5 days a week for 28 days with a graduated dose ranging from 45 mg/Kg BW to 5625 mg/kg BW. The results obtained are the last treatment no rat died. Based on the measurement of hemoglobin values obtained between 12.57 to 17.97 g/dL, hematocrit values obtained between 31.77 to 51.57% and leukocyte counts obtained between 5.01 to 23.22x103 cells/μL were still in the normal range. The conclusion of this study is that a dose greater than 5,625 mg/kg BW is the LD50 value for the ethanol extract of 70% temulawak. Giving 70% ethanol extract of temulawak until a dose of 5,625 mg/kg BW for 28 days to male and female rats had a decreasing effect on hemoglobin, hematocrit, and leukocyte values, but the values were still within normal limits. There is no significant difference between male and female rats in the measurement results of hemoglobin, hematocrit, and leukocyte values. 
Antiproliferative Potency of God’s Crown Fruit (Phaleria macrocarpa) Extract Against Breast Cancer Cell Hasim Hasim; Yonathan Arderian Mantik; Husnawati Husnawati; Bambang Pontjo Priosoeryanto; Ratna Puspita
Borneo Journal of Pharmacy Vol. 5 No. 4 (2022): Borneo Journal of Pharmacy
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjop.v5i4.2822

Abstract

Breast cancer is a sickness caused by abnormal cell growth in the breast. Mahkota Dewa fruit or god’s crown fruit products (Phaleria macrocarpa) contain flavonoids, alkaloids, polyphenols, and tannins associated with active compounds. This work directs to influence the potency of P. macrocarpa fruit as an antiproliferative agent against breast cancer cells (MCF-7 cells). The antiproliferative potency of P. macrocarpa fruit was proved by extracting and fractionating P. macrocarpa fruit using maceration. The cytotoxicity of extracts and fractions was determined using Brine Shrimp Lethality Test (BSLT). The antiproliferative potency against MCF-7 cells was tested using the hemacytometer approach. This work demonstrates the crude ethanol extract, n-hexane fraction, ethyl acetate fraction, and water fraction. The LC50 values in crude ethanol extract, n-hexane fraction, ethyl acetate fraction, and water fraction were 13.72 ppm, 147.55 ppm, 405.81 ppm, and 149. 07 ppm severally. Phaleria macrocarpa fruit has shown antiproliferation potency against MCF-7 cells. The maximum part of crude ethanol extract antiproliferative potency (56 ppm) effectively suppressed MCF-7 cell growth by 70. 9% while doxorubicin (100 ppm) by 46. 92%. This work confirms that crude ethanol extract of P. macrocarpa fruit interacts synergistically as an antiproliferative compound against MCF-7 cells.
Co-Authors Agus Setiyono Akhmad Endang Zainal Hasan Aldi Fadilah Amrista Fanzani Kananga Aziz Syamsul Huda Bambang Pontjo Priosoeryanto Chintia Ayu Puspita Danty Oktiana Prastiwi DIMAS ANDRIANTO Elgiani Yassifa Yulia Nur Insari Eny Ida Riyanti Faisal Rahman Galuh Rizal Prayoga Gina Oktaviani Sabrina Hanif Alamudin Manshur Hasim Hasim Hasim Hasim Heddy Julistiono Ika Yuni Astutik Imelia Dewi Irmanida Batubara LAKSMI AMBARSARI Latifah Kosim Darusman Mega Safithri Muhammad Farhan Muhammad Irfansyah Pradika Nurliani Bermawie Ratna Puspita Safira Rahmadanti Santi Nuraeni Siti Wachidatun Zulaikha Slamet Hamzah Sri Wahyuni Syndilona Br Sitepu Tohru Mitsunaga Tommy Abrar Yonathan Arderian Mantik Zulfikar Muchammad