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I Nyoman Arsana
Program Studi Biologi, Fakultas Teknologi Informasi Dan Sains, Universitas Hindu Indonesia
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Antibacterial activity of ethanolic extract of Averrhoa bilimbi L. fruit against Salmonella typhi Ni Made Riris Divayani Aristyantari; Ni Ketut Ayu Juliasih; Ni Luh Gede Sudaryati; I Nyoman Arsana
Indonesian Journal of Pharmacology and Therapy Vol 3 No 2 (2022)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.4431

Abstract

Averrhoa bilimbi L. fruit has been used in Bali traditional medicine to treat typhoid fever. The fruit contains flavonoids and triterpenoids which are considered to have antibacterial activity. This study aimed to investigate antibacterial activity of ethanolic extract of A. bilimbi L. fruit. The ethanolic extract was prepared by maceration and antibacterial activity was determined by the disc diffusion method against Salmonella typhi cultured on Mueller hinton agar (MHA). Ciprofloxacin was used as positive control and sterile distilled water as negative control. The results showed that the ethanolic extract of A. bilimbi L. fruit at various concentrations of 25; 50; 75 and 100% have an inhibition zone diameter of 0 ± 0; 13.000 ± 1.414; 18.750 ± 1.500 and 20.250 ± 1.707 mm, respectively. In addition, ciprofloxacin at concentration of 1 mg/L has an inhibition zone diameter of 34.250 ± 1.892 mm. In conclusion, the ethanolic extract of A. bilimbi L. fruit has strong antibacterial activity against S. typhi.
Loloh Daun Kemangi (Ocimum basilicum L.) Tidak Cukup Kuat Sebagai Minuman Antioksidan Untuk Memperkuat Sistem Pengobatan Tradisional I Nyoman Arsana; A.A.Ayu Sauca Sunia Widyantari; I Ketut Winantra; Ni Putu Dyah Sartika Sari; Ni Made Ayu Suwandani; Ni Ketut Ayu Juliasih
Metamorfosa: Journal of Biological Sciences Vol. 11 No. 01 (2024)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2024.v11.i01.p7

Abstract

Total Flavonoid dianalisis dengan menggunakan larutan standar quarsetine. Tanin dianalisis dengan menggunakan pereaksi Folin-Denis, aktivitas antioksidan dianalisis dengan menggunakan 2,2-diphenyl-1-picrylhydrazyl (DPPH) sebagai sumber radikal bebas, dan antibakteri dengan metode difusi pada Mueller Hinton Agar (MHA). Hasil penelitian menunjukkan total fenol, total flavonoid, tanin, dan IC50 loloh VB berturut-turut adalah 48,78 mgGAE/100g, 107,31 mg QE/100g, 72,24 mg/100 g, dan 3553,37 ppm. Sedangkan VL berturut-turut sebasar 91,40 mgGAE/100g, 305,65 mg QE/100g, 153,43mg/100g, dan 1507,85ppm. Loloh VB maupun VL belum mampu untuk menghambat pertumbuhan bakteri baik bakteri Gram-positif maupun Gram-negatif. Namun, ekstrak etanol VB dan VL mampu menghambat bakteri Gram-positif tetapi tidak bakteri gram negatif. Kesimpulan, loloh VB dan VL belum cukup kuat sebagai minuman yang kaya senyawa bioaktif dengan kemampuan sebagai antioksidan maupun antibakteri.
Loloh Daun Kemangi (Ocimum basilicum L.) Tidak Cukup Kuat Sebagai Minuman Antioksidan Untuk Memperkuat Sistem Pengobatan Tradisional I Nyoman Arsana; A.A.Ayu Sauca Sunia Widyantari; I Ketut Winantra; Ni Putu Dyah Sartika Sari; Ni Made Ayu Suwandani; Ni Ketut Ayu Juliasih
Metamorfosa: Journal of Biological Sciences Vol. 11 No. 01 (2024)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2024.v11.i01.p7

Abstract

Total Flavonoid dianalisis dengan menggunakan larutan standar quarsetine. Tanin dianalisis dengan menggunakan pereaksi Folin-Denis, aktivitas antioksidan dianalisis dengan menggunakan 2,2-diphenyl-1-picrylhydrazyl (DPPH) sebagai sumber radikal bebas, dan antibakteri dengan metode difusi pada Mueller Hinton Agar (MHA). Hasil penelitian menunjukkan total fenol, total flavonoid, tanin, dan IC50 loloh VB berturut-turut adalah 48,78 mgGAE/100g, 107,31 mg QE/100g, 72,24 mg/100 g, dan 3553,37 ppm. Sedangkan VL berturut-turut sebasar 91,40 mgGAE/100g, 305,65 mg QE/100g, 153,43mg/100g, dan 1507,85ppm. Loloh VB maupun VL belum mampu untuk menghambat pertumbuhan bakteri baik bakteri Gram-positif maupun Gram-negatif. Namun, ekstrak etanol VB dan VL mampu menghambat bakteri Gram-positif tetapi tidak bakteri gram negatif. Kesimpulan, loloh VB dan VL belum cukup kuat sebagai minuman yang kaya senyawa bioaktif dengan kemampuan sebagai antioksidan maupun antibakteri.
Keragaman Tanaman Obat dalam Lontar “Taru Pramana” dan Pemanfaatannya untuk Pengobatan Tradisional Bali I Nyoman Arsana
Jurnal Kajian Bali (Journal of Bali Studies) Vol. 9 No. 1 (2019): WACANA KRITIS BUDAYA BALI
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JKB.2019.v09.i01.p12

Abstract

“Taru Pramana” lontar manuscripts contain medicinal plant diversity and it uses for traditional system of Balinese medicine. This study aims to describe medicinal plant diversity in “Taru Pramana” lontar manuscripts and its use for traditional system of Balinese medicine. This research uses the library method. The unit of analysis is the “Taru Pramana” lontar manuscript from collection of the Leiden University Library, Netherlands. The data obtained were analyzed descriptively. The ethnosystem approach is used as the main approach in this analysis, which places an emic perspective on the relationship between humans and plants. A total of 182 plant species are used in traditional Balinese medicine, mostly including family members of euphorbiaceae, moraceae, fabaceae, and zingiberaceae. The parts used were leaves, shoots, stem bark, roots, fruit, sap, tuber and used them into various forms of medicine such as loloh, boreh, sembar, tutuh, tempel, and ses. The practice of the use of medicinal plants in traditional Balinese medicine is based on a strong belief and knowledge system.
Co-Authors A.A.Ayu Sauca Sunia Widyantari and I. B. Putra-Manuaba Angga Baskara Arisandi Arisandi Ayu Meiliana Laksmi Dewi Dewa Ayu Made Novia Dewi Fauzi Rahmat Kurniawan I Gede Ketut Adiputra I Gusti Ayu Ketut Artatik I K. P Astawa I KETUT WINANTRA I Made Agus Gelgel Wirasuta I Putu Sudiartawan I W. Wahyudi I Wayan Martadi Santika I Wayan Suarda Ida Ayu Gede Winda Savitri Ida Bagus Putra Suta Ida Bagus Wiryanatha J. A. Pangkahila Luh Putu Arishanti W. Melia-Arisanti N. K. N. Adiputra N.K.A. Juliasih Ni Kadek Warditiani Ni Ketut Ayu Juliasih Ni Ketut Ayu Juliasih Ni Komang Madiasih Ni Luh Gede Sudaryati Ni Made Ayu Suwandani Ni Made Riris Divayani Aristyantari Ni Made Widiastuti Ni Nyoman Sri Puspa Adi Ni Nyoman Triyani Ni Putu Dyah Sartika Sari Pande Kadek Yusika Ryanita Pande Made Nova Armita Putu Alit Wardana Putu Lakustini Cahyaningrum Sudaryati N.L.G. Sumarya I M.