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Milk production and quality of Etawa crossbreed dairy goat that given Tithonia diversifolia, corn waste and concentrate based palm kernel cake Arief ARIEF; RUSDIMANSYAH RUSDIMANSYAH; SIMEL SOWMEN; RONI PAZLA; RIZQAN RIZQAN
Biodiversitas Journal of Biological Diversity Vol. 21 No. 9 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210910

Abstract

Abstract. Arief, Rudimansyah, Sowmen S, Rizqan RP. 2020. Milk production and quality of Etawa crossbreed dairy goat that given Tithonia diversifolia, corn waste and concentrate based palm kernel cake. Biodiversitas 21: 4004-4009. The research aims to evaluate the production and quality of goat milk of Etawa crossbreed dairy goat (ECDG) which is fed by Tithonia diversifolia (T), corn waste (CW), and concentrate-based palm kernel cake (CBP),. The experimental design used in this study was a completely random design (CRD) with four times treatment rations and five times replications. There are five treatments used in this research which are; treatment A used 60 % T + 40 % tofu-dregs; B used 60 % T + 20% tofu-dregs + 20% CBP; C used 45 % T + 15% CW + 20% tofu-dregs + 20% CBP; D used 30 % T + 30% CW + 20% tofu-dregs + 20% CBP. The data were analyzed by Analysis of Varian (ANOVA) and Duncan's Multiple Range Test (DMRT) for further tests. The parameters measured were milk production and milk quality, which covers dry matter, water content, protein, fat, solid non-fat, and lactose. The result showed that the treatment gave no significant effect (P> 0.05) of milk production, dry matter, protein, fat, solid non-fat, and lactose. The study concluded that the use of T. diversifolia, corn-waste, and CBP could maintain milk production quality.
Short Communication: Identification of the exon 1 myostatin gene polymorphism and its association with slaughtered weight in Indonesian Kampung and Broiler Chicken Linda Suhartati; ISYANA KHAERUNNISA; ASEP GUNAWAN; RUKMIASIH RUKMIASIH; SRI DARWATI; CECE SUMANTRI; RIZQAN RIZQAN
Biodiversitas Journal of Biological Diversity Vol. 21 No. 8 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210860

Abstract

Abstract. Suhartati L, Khaerunnisa L, Gunawan A, Rukmiasih, Darwati S, Sumantri C, Rizqan. 2020. Short Communication: Identification of the exon 1 myostatin gene polymorphism and its association with slaughtered weight in Indonesian Kampung and Broiler Chicken. Biodiversitas 21: 3893-3897.  Myostatin is widely known as a negative regulator of skeletal muscle development by controlling hypertrophy and hyperplasia. Silent mutation in exon 1 (c.234G>A) was then thought to be associated with chicken growth performances. This study was designed to analyze polymorphisms of c.234G>A mutation of the myostatin gene and its effect on slaughtered weight in Indonesian Kampung and meat-type chicken. The SatI restriction enzyme was used for genotype determination through the PCR-RFLP technique. The effect of genotype on the slaughtered weight was analyzed using the General Linear Model (GLM) procedure. Genotyping was performed on 138 chicken from Kampung (n=67) and meat type (cobb Broiler) (n=71) chicken. The amplification product contained 226 bp nucleotides. This myostatin gene was polymorphic in both chicken population, produced 2 alleles (G and A), and 3 genotypes (GG, GA, and AA). The A allele had a higher frequency than the G allele in all populations. In this study, genotype of AA and GA had higher live weight, carcass weight, breast weight, thigh weight, drumsticks weight, wings weight, breast muscle weight, thighs muscle weight, drumsticks muscle weight than GG genotype of 12 weeks-old Kampung chicken. The results suggested that the c.234G>A mutation in exon 1 could be potentially recommended as a genetic marker for chicken slaughtered weight traits.
Potential of dadiah kapau from Agam District, West Sumatra, Indonesia as a source of probiotics for health ELLY ROZA; SALAM N. ARITONANG; YULIA YELLITA; HILDA SUSANTY; RIZQAN RIZQAN; YUDHA ENDRA PRATAMA
Biodiversitas Journal of Biological Diversity Vol. 23 No. 1 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230161

Abstract

Abstract. Roza E, Aritonang SN, Yellita Y, Susanty H, Rizqan, Pratama YE. 2021. Potential of dadiah kapau from Agam District, Indonesia as a source of probiotics for health. Biodiversitas 23: 564-571. Dadiah is a fermented product of buffalo milk prepared in a bamboo container as a traditional food in the Minangkabau tribe of West Sumatra, Indonesia. This study aimed to identify lactic acid bacteria that are potentially probiotics from dadiah products. The method used in this research includes an experimental survey with descriptive data analysis. The dadiah used in this study was taken from dadiah breeders and processors in Nagari Kapau, Agam District, West Sumatra, Indonesia. Tests for selection of probiotic candidates included macroscopic and microscopic observations, catalase test, fermentative type, resistance to gastric acid (pH 3), resistance to bile salts, antimicrobial activity using test bacteria Escherichia coli O157, Staphylococcus aureus, Listeria monocytogenes and Salmonella sp. Molecular identification was carried out by using 16S rRNA to determine the relationship of species using MEGA version 7.0 application. The study results revealed that the total colonies of dadiah kapau lactic acid bacteria were 128 x 108 CFU/g, and 12 isolates were obtained from the isolation results. Catalase negative for 12 isolates and 9 homofermentative types and 3 including heterofermentative types. Tests for resistance to gastric acid (pH 3) and bile salt resistance, the best viability was 84.61% and 86.51% respectively. The best antimicrobial activity showed a clear zone against the test bacteria E. coli O157, S. aureus, L. monocytogenes, and Salmonella sp. The phylogenetic analysis of the sequencing results shows that the dadiah kapau lactic acid bacteria are closely related to Lactobacillus plantarum.
Characterization of lactic acid bacteria from buffalo dairy product (dadiah) as potential probiotics SALAM N. ARITONANG; ELLY ROZA; YETMANELI YETMANELI; AFRIANI SANDRA; RIZQAN RIZQAN
Biodiversitas Journal of Biological Diversity Vol. 23 No. 9 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230906

Abstract

Abstract. Aritonang SN, Roza E, Yetmaneli, Sandra A, Rizqan. 2022. Characterization of Lactic Acid Bacteria from Buffalo Dairy Product (Dadiah) as Potential Probiotics. Biodiversitas 23: 4418-4423. Dadiah is one of fermented milk from West Sumatra which made from buffalo milk. These product made traditionally by pouring fresh buffalo milk into a bamboo stick covered with banana leaves and fermenting at room temperature for two days. The study's objective was to isolate and identify Lactic Acid Bacteria (LAB) from dadiah as candidates for probiotics. The factors that are considered are LAB Characterization, Biochemical and Microscopic Characterization of LAB Isolate, Bacterial Resistance in Acidic conditions, Bile Salts Resistance, and Antimicrobial Activity. Furthermore the isolate identify by Polymerase Chain Reaction (PCR) using 16S rRNA. Samples of dadiah were collected from five farmers in Tanjung Bonai Village, North Lintau Buo Regency, Tanah Datar District, West Sumatra, Indonesia. Observations were made in four replications, and the data obtained were analyzed descriptively. The total colony of lactic acid bacteria from dadiah was 7x108-23x108 CFU/mL. LAB isolates from dadiah were classified as Gram-positive bacilli. The isolates are resistant to acidic conditions and bile salts and have inhibitory activity against pathogenic bacteria. Identifying the selected isolate using the 16S rRNA showed that the isolate had similarities with Lactobacillus casei strain HDS-01. It can be concluded that dadiah used in this study is as a probiotic food.