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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Veteriner Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Journal of Tropical Biodiversity

Sri Darwati

Department Production Science And Technology, Animal Husbandry Faculty, IPB University, West Java, Indonesia.

Author-ID : 2865790

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Immunology & microbiology Veterinary Other

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Articles

Pertumbuhan Bulu Ayam Hasil Persilangan Sentul dengan Onagadori dan Resiprokal Umur 1 sampai 12 Minggu Achmad Gitaraka Archimilar; Sri Darwati; Cece Sumantri
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 6, No 1 (2019): JITRO, Januari
Publisher : Universitas Halu Oleo

ABSTRAKAyam tidak hanya sebagai komoditi pangan, namun juga hias. Salah satu ayam hias yang unik dan banyak menarik minat pecinta ayam hias adalah ayam ekor panjang atau onagadori. Salah satu jenis asli ayam lokal Indonesia adalah ayam sentul. Ayam sentul adalah ayam asli dari daerah Ciamis, Jawa Barat. Persilangan onagadori dengan sentul dan resiprokalnya diharapkan memiliki bulu yang panjang di bagian tubuhnya dengan perpaduan warna bulu abu-abu akibat adanya kombinasi warna dari kedua tetua dengan performa tubuh yang gagah dan indah. Parameter yang diukur dalam penelitian ini adalah pertumbuhan bulu sayap, pinggang, dan ekor pada umur 1-12 minggu. Pada penelitian ini digunakan rancangan acak kelompok untuk menganalisa data. Hasil penelitian menunjukan bahwa panjang bulu sayap SO jantan 7,93±0,63 cm, SO betina 7,96±0,79 cm dan OS jantan 6,39±0,66 cm, OS betina 6,29±0,91 cm. Hasil pengukuran bulu pinggang SO jantan 6.80±0.10 cm, SO betina 6,81±0,10 cm dan OS jantan 2,59±0,86 cm, OS betina 2,50±0,98 cm. Panjang bulu ekor SO jantan 10,71±0,10 cm, SO betina 10,78±0,10 cm dan OS jantan 7,31±0,10 cm, OS betina 7,29±0,12 cm. Karakteristik kualitatif ayam hasil persilangan onagadori dengan sentul pada warna bulu jantan cenderung lebih dominan 1 warna sedangkan yang betina 2 warna. Warna shank yang dihasilkan dari persilangan ini adalah abu dan putih lebih banyak dibandingkan hijau. Bentuk jengger hasil persilangan ini bentuk jengger pea lebih banyak daripada bentuk jengger single dan cushion. Ayam hasil persilangan SO memiliki pertambahan pertumbuhan bulu sayap, pinggang, dan ekor lebih cepat dan panjang dari OS.Kata kunci: onagadori, pertumbuhan bulu, resiprokal, sentulABSTRACTChicken is not only a food commodity, but also ornamental. One of the unique ornamental chickens that attracts many ornamental chicken lovers is the long-tailed chicken or onagadori. One native of Indonesian local chicken is sentul chicken from the Ciamis area, West Java. The crossing of cock onagadori with hen sentul (Onagadori-Sentul/OS) and its reciprocal cock sentul with hen onagadori (Sentul-Onagadori/SO) is expected to have long feather tail, and on its body with a combination of gray feather due to the combination of colors from the two parents with a good performance.. Parameters measured in this study were the growth of wing, saddle, and tail feathers at 1-12 weeks. A randomized block design is used to analyze this data. The measurement of the males 6.80±0.08 cm, SO females 6.81±0.08 cm, and OS males 2.59±0.86 cm, Females OS 2.50±0.98 cm. Result of SO tail tailed data 10.71±0.10 cm, SO females 10.78±0.10 cm and OS males 7.31±0.10 cm Females OS 7.29±0.12 cm. Chicken offspring from SO crossbreed has better feather growth than onagadori. While the offspring from OS crossbreed has better feather growth than sentul chicken. Characteristics of qualitative chicken onagadori crosses with sentul on the color of male feathers tend to be more dominant one color while the female two colors. The shank color resulting from this cross color of the ash and white shank is more dominant than green. The pea comb form produced from this cross is more dominant than the single and cushion comb. The SO cross breed chikens have faster feather growth of wing, saddle, and tail than OS chikens.Key words: feathers growth, onagadori, reciprocal, sentul

Identifikasi Keragaman Gen Kalpastatin (CAST) pada Ayam Lokal Indonesia (IDENTIFICATION OF POLYMORPHISM CALPASTATINE GENE IN LOCAL CHICKEN) Ahmad Saleh Harahap; Cece Sumantri; Niken Ulupi; Sri Darwati; Tike Sartika
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Calpastatin (CAST) gene is one of the genes that play a role in the process of tenderization of meat. CAST gene serves as an inhibitor of meat tenderness. The purpose of this study was to identify Single Nucleotide Polymorphisms (SNP) in the CAST gene of kampong chickens. A total of 61 kampong chickens used were used in the study including strain cobb, F1 of strain cobb-kampong, merawang, sentul, nunukan, and pelung, respectively. The method used is extracted DNA from blood samples, then amplified by Polymerase Chain Reaction (PCR) and then genotyping by DNA sequencing. The results showed that SNP was identified at position g.42988G> T in the intron 11 CAST gene of merawang chicken with genotype GG and GT. SNP was not identified in kampong chicken, strain cobb, F1 of strain cobb-kampong, sentul, nunukan, and pelung chicken, respectively. In conclusion, the intron 11 CAST gene region with 482 of product length of merawang chicken is polymorphic, whereas the other chicken species is monomorphic. ABSTRAK Gen Calpastatin (CAST) merupakan salah satu gen yang berperan dalam proses keempukan pada daging. Gen CAST berfungsi sebagai inhibitor (penghambat) keempukan daging. Tujuan dari penelitian ini adalah mengidentifikasi Single Nucleotide Polymorphisms (SNP) gen CAST pada ayam kampung. Jumlah ayam yang digunakan 61 ekor ayam kampung, ayam strain cobb, F1 ayam strain cobb-kampung, ayam merawang, ayam sentul, ayam nunukan, dan ayam pelung. Metode yang dilakukan adalah ekstrasi DNA dari sampel darah, kemudian dilakukan amplifikasi menggunakan mesin Polymerase Chain Reaction (PCR) dan selanjutnya dilakukan genotiping melalui DNA sequencing. Hasil penelitian menunjukkan bahwa gen CAST intron 11 pada ayam pengamatan ditemukan SNP posisi g.42988G>T pada ayam merawang dengan genotip GG dan GT, sedangkan pada ayam kampung, ayam strain cobb, F1 ayam kampung dengan strain cobb, ayam sentul, ayam nunukan, ayam merawang dan ayam pelung tidak ditemukan SNP. Simpulan dari penelitian ini memperlihatkan gen CAST daerah intron 11 dengan panjang produk 482 pada ayam merawang bersifat polimorfik, sedangkan jenis ayam yang lain bersifat monomorfik.

Efektivitas Low Density Lipoprotein dan Kuning Telur Ayam dan Puyuh pada Pengawetan Semen Ayam Merawang (EFFECTIVESS OF LOW DENSITY LIPOPROTEIN AND EGG YOLK FROM CHICKEN AND QUAIL ON MERAWANG SEMEN PRESERVATION) Magfira Magfira; Raden Iis Arifiantini; Ni Wayan Karniani Karja; Sri Darwati
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The successful of artificial insemination (AI) depends on the semen quality and extender. To minimize effect of cold shock during storage, extender is added with egg yolk. The objectives of this study were to compare the effectiveness of pure Low Density Lipoprotein (LDL) and egg yolk from domestic chicken and quail on motility and longevity of Merawang chicken sperm. The semen was collected by massage method from three Merawang roosters. Immediately after collection, semen was evaluated macroscopically and microscopically. Only semen demonstrated >70% motility and <20% sperm abnormality were used in this study. Semen divided into four aliquots and diluted with Lactate Ringer (LR) LDL chicken (RL-LDL-C), LR-LDL quail (LR-LDL-Q), LR- chicken Egg Yolk (LR-CEY), Ringer Lactate quail Egg Yolk (RL-QEY). Diluted semen than stored at 5oC. Sperm motility was examined twice a day and the longevity of sperm was determined every day until the sperm reach 0% motility. The motility of spermatozoa in the LR-LDL diluent differed from the sperm motility in the RL-QEY diluent at the 60th and 72th hour (P <0.05) poststorage. However, there was no difference in motility sperm in LR-LDL-C, RL-LDL-Q and RL-CEY. Additionally, there is no difference (P> 0.05) in spermatozoa longivity in the four diluents, with a range of longivities between 4.43 to 5.93 days. ABSTRAK Keberhasilan inseminasi buatan (IB) salah satunya bergantung pada kualitas semen dan pengencer yang digunakan. Dalam meminimalisir pengaruh cold shock saat penyimpanan, pengencer ditambahkan dengan kuning telur. Tujuan dari penelitian ini adalah untuk membandingkan efektivitas Low Density Lipoprotein (LDL) dan kuning telur yang berasal dari ayam kampung dan puyuh terhadap motilitas dan longivitas spermatozoa ayam. Koleksi semen dilakukan menggunakan metode pemijatan pada tiga ekor ayam merawang. Setelah semen dikoleksi, selanjutnya semen dievaluasi secara makroskopis dan mikroskopis. Semen yang menunjukkan motilitas 70% dan abnormalitas kurang dari 20% dibagi empat dan diencerkan menggunakan Ringer Laktat-LDLA (RL-LDLA), Ringer Laktat-(RL-LDLP), Ringer Laktatkuning telur ayam (RL-KTA), dan RL-kuning telur puyuh (RL-KTP). Semen yang telah diencerkan kemudian disimpan pada suhu 5oC. Motilitas spermatozoa diamati dua kali sehari sampai motilitas mencapai 0%. Motilitas spermatozoa dalam pengencer RL-LDLA berbeda dengan motilitas spermatozoa dalam pengencer RL-KTP pada jam ke-60 dan ke-72 (P<0.05) pascapenyimpanan. Akan tetapi tidak terdapat perbedaan motilitas spermatozoa dalam RL-LDLA, RL-LDLP dan RL-KTA. Longivitas spermatozoa dalam empat pengencer tidak terdapat perbedaan (P>0.05) dengan rentang longivitas antara 4,43 sampai 5,93 hari.

Karakteristik Genetik dan Fenotip Ayam Nunukan di Pulau Tarakan, Kalimantan Timur (THE PHENOTHYPIC AND GENETIC CHARACTERISTIC OF NUNUKAN CHICKEN OF TARAKAN ISLAND, EAST BORNEO) Muhammad Alwi; Cece Sumantri; Sri Darwati
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.701 KB)

Nunukan chicken is local chicken of Tarakan and Nunukan in East Borneo Province. It is a germplasmof East Kalimantan which have special characteristics. Due to its characteristic, the chicken extremelyneed to be conserved and develop. This study was conducted to find out more reliable data on thecharacteristics of nunukan chicken in Tarakan Island. It was done by directly observed and measuredfenotype traits of nunukan chicken. Sample used was mature chicken, with the total tnumber 211 birds.Fenotype qualitative traits of nunukan chicken was florid brown of feather basic colour, columbian offeather colour theme, golden of feather flickering, and solid of feather pattern. The other special traits islittle growth (even not growth) of wings and tail feather, and on immature chicken (age of 0-5 month)commonly primary feather not growth. There was 13 fenotype quantitative traits on male, and two onfemale chicken was different between location (sub district) on 20 traits. The number of nunukan chickenat Tarakan Island was about 940 birds, and 36,17% was mature chicken. The effective population totalwas 299 birds, highest effective population was in West Tarakan (162 birds) and lowest was in MidleTarakan (46 birds). The average of inbreeding increased 0.177%, highest was in Midle Tarakan (1,08%)and lowest was in West Tarakan (0,31). The population of nunukan chicken in Tarakan Island was specificof the fenotype characteristic.

Penilaian Kandang Sehat dan Produktif Domba di Desa/Kelurahan Lingkar Kampus Institut Pertanian Bogor, Darmaga Rahmat Hidayat; Kukuh Santoso; . Suryahadi; Sri Darwati; Agik Suprayogi; . Prastowo
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 1 No. 1 (2015): Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.1.1.20-27

Institute for Research and Community Service Bogor Agricultural University (LPPM IPB) has excellent performance in community service activities (PPM). LPPM IPB since 2009 has conducted various community service activities in rural and urban campus area around of Campus. One of the activities is related with sheep and supporting activities. This assessment activity has two (2) purposes: first, to measure the knowledge of farmers in the application of science and technology especially cage and cultivation that has been given, and the second one, to give appreciation for the achievements of farmers in the management of sheep, especially for the category of healthy and productive cage. This activity was carried out by field visits and interviews using a special form of assessment. 17 breeders were determined based on nonprobability purposive sampling method. The highest value obtained Nurpandi farmer from the Village Sukawening got 920.9 points, while the lowest value obtained Sahedi farmer from the Village Purwasari got 308.3 points. Conclusions from the results obtained showed mostly cage sheep (52.94%) have not yet reached the category of healthy and productive cage (final value of less than 800 points).

Identification of Polymorphism Growth Hormone Gene in Local Chickens Resulting from Three Breed Crosses Harini Nurcahya; Sri Darwati; Isyana Khaerunnisa; Ida Wiryanti
Journal of Tropical Biodiversity Vol 1 No 2 (2021): April 2021
Publisher : Universitas Nasional Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (427.176 KB)

Local chicken has an important role as a source of animal protein producing eggs and meat. Local chicken has the advantage of being able to adapt to hot weather and be more resistant to disease. However, there are still weaknesses, namely relatively slow growth and relatively low egg production. Therefore, one way to overcome these weaknesses is by holding selection and crossing. Local chickens used for crossing in this study were sentul, kampung and kedu chicken. In connection with the growth of chickens that are slow and low in productivity of local chickens, it is necessary to examine the factors that influence growth and productivity. The hormones that have an effect on growth are GH (Growth Hormone), so that the characteristics of the gene of growth in local chickens from the cross can be known.The purpose of this study was to detect the characteristics of the Growth Hormone gene in local chickens resulting from Three Breed Crosses . The research material used in this study was forty local chickens produced by cross-sentul, kampung and kedu chicken. To find out GH gene polymorphism is by PCR-RFLP with cutting enzyme EcoRV.The results of the GH gene study in sentul, kampung and keducrossed chickens are polymorphic and are in the Hardy-Weinberg equilibrium state. Kampung chicken cross population was polymorphic for GH|EcoRV loci and the GG genotype was predominant in this population. G allele frequency in kampung,kedu and sentul chicken cross population is higher than the A allele. The highest genetic diversity was observed at kampung,kedu and sentul chicken cross and the heterozygosity value was lowcategory.

Productivity of IPB-D2 and IPB-D3 Chickens with Repeatability of Newcastle Disease Antibody Titer Ratnawati, Diana; Sri Darwati; Murtini, Sri; Sumantri, Cece
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 1 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.1.140

ND virus causes mortality in poultry. Passively acquired maternal antibodies inhibit immunoglobulin formation. Repeatability is a genetic parameter that determines the inheritance of traits from elders to chicks. IPB-D2 chickens were selected for disease resistance, and IPB-D3 chickens were selected for weight gain. This study aimed to evaluate the productivity of IPB-D2 and IPB-D3 chickens and assess the inheritance of Newcastle Disease (ND) antibody traits in 36 IPB-D3 and 21 IPB-D2 chickens. The T-test was used to compare the group means of the two chicken breeds. Antibody titer measurements were based on the Geometric Mean Titer (GMT). Estimation of ND titer repeatability using within-class correlation. Fertility and hatchability differed significantly (p < 0.05). ND titer of IPB-D2 chicken and IPB-D3 chicken DOC LOG 2 GMT 1.61 ± 1.10 and 1.34 ± 0.95. The antibody titer of IPB-D2 and IPB-D3 chickens at 14 days of age was 1.02 ± 1.20 and 1.37 ± 0.95. The ND titer value in the egg yolk of IPB-D2 chicken was 4.02 ± 1.94, and in IPB-D3 chicken was 3.64 ± 2.54. The results showed the repeatability value of l antibodies in IPB D-2 chickens and IPB D-3 chickens in DOC 0.49±0.30, 0.42±0.33 and 0.39±0.28; 0.25±0.15, respectively. Fertility and hatchability of IPB-D3 chickens were better than those of IPB-D2 chickens. The yolk ND titer of IPB-D2 chickens was higher than IPB- D3 chicken. The ND titer reciprocity of IPB-D2 chickens was higher than that of the IPB-D3 chickens. Keywords: IPB-D2 Chicken, IPB-D3 Chicken, Newcastle Disease, productivity, repeatability

Determination of the New SNP g.939A>G of the TYR Gene in Abnormal Coat Color in Bali Cattle Kholijah; Mokhmad Fakhrul Ulum; Sri Darwati; Ronny Rachman Noor; Jakaria
Jurnal Ilmu-Ilmu Peternakan Vol. 34 No. 1 (2024): April 2024
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2024.034.01.05

This study aimed to assess the presence of single nucleotide polymorphisms (SNPs) in the exon 1 region of the tyrosinase (TYR) gene in Bali cattle with abnormal coat color. This study analyzed 43 Bali cattle, 26 individuals with typical or standard coat color, and 17 individuals with albinism. The genetic diversity of the TYR gene was determined via direct sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) techniques. The sequence data of the TYR gene were scrutinized using BioEdit and MEGA10 software to detect single nucleotide polymorphisms (SNPs) related to different types of coat color features in Bali cattle. The genetic diversity information was derived from computations performed using PopGen 1.32 software. The results showed that exon 1 of the TYR gene was affected by the new SNP g.939A>G, which is polymorphic in Bali cattle. In conclusion, the SNP c.939A>G can be further analyzed for use as a candidate-assisted selection (MAS) for abnormal coat color in Bali cattle.

External Quality Of Merawangarab Chicken Eggs With Different Genetic Composition Rafif Nurwansyah; Harini Nurcahya; Ikhsan Matodang; Sri Darwati
Journal of Tropical Biodiversity Vol 6 No 1 (2025): December 2025
Publisher : Universitas Nasional Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59689/bio.v6i1.329

Eggs, as a food ingredient, possess complete nutritional value that is beneficial for human health, are readily available, affordable, and popular with the public. Likewise, hatching eggs provide the nutrients needed for the development of chicken embryos. Therefore, eggs for consumption as food and hatching eggs. The external quality of eggs is one indicator of egg quality. Merawang chickens and arab chickens are local Indonesian chickens. Crossbreeding of the two chickens is expected to produce superior offspring in production and egg quality. This study aimed to evaluate the external quality of merawangarab crossbred chicken eggs with different merawangarab genetic compositions (M), namely 25% M, 50% M, 62.5% M, and 75% M with a total of 30 hens. The materials used were eggs from merawangarab crossbred chickens. This study used a randomized block design (RAK) and continued with Tukey's further test. The external quality of eggs observed was shape index, egg weight, shell weight, and shell ratio. Different genetic compositions did not affect the external quality of merawangarab crossbred chicken eggs. Eggs from four crossbred merawangarab chickens were (25% M, 50% M, 62.5% M and 75% M) classified as small

Co-Authors . Prastowo Achmad Gitaraka Archimilar Agik Suprayogi Cece Sumantri Harahap, Ahmad Saleh Harini Nurcahya Harini Nurcahya Mariandayani Ida Wiryanti Ikhsan Matodang Isyana Khaerunnisa Jakaria Jakaria Kholijah Kukuh Santoso Magfira Magfira Mokhmad Fakhrul Ulum Muhammad Alwi Ni Wayan Karniani Karja Niken Ulupi R. Iis Arifiantini Rafif Nurwansyah Rahmat Hidayat Ratnawati, Diana Ronny Rachman Noor sri murtini . Suryahadi (Suryahadi) TIKE SARTIKA

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