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All Journal BioWallacea Journal of Biological Research Jurnal Pengabdian Masyarakat (ABDIRA)
Suriana Suriana
Jurusan Biologi Fakultas MIPA Universitas Halu Oleo, Department Of Biology, Faculty Of Mathematics And Natural Sains Halu Oleo University
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Karakteristik Segmen Gen sitokrom C Oksidase Subunit I (COI) Ngengat Plusia chalcites (Lepidoptera: Noctuidae) Suriana Suriana; Marwansyah Marwansyah; Amirullah Amirullah
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 6, No 2 (2019): BioWallacea and Biotechnological Science
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (579.901 KB) | DOI: 10.33772/biowallacea.v6i2.8824

Abstract

ABSTRACTThe research was exploration ofpartial COI gene of moth (Plusia chalcites).The aim of this research is to characterization of nucleotide sequence of these COI gene, that was exracted with Cetyl Trimetyl Ammonium Bromide (CTAB) method, and then amplified by Polymerase Chain Reaction (PCR) technique with spesifik primer. The result PCR amplified subsequent analysis of nitrogen base arrangement in these COI gene. The analysis data using the BioEdit and MEGA Version 5.0 software.The results showed that the  segment of COI gene in Plusia chalcites as long 710 bp (base pairs). From these, there are 503/657 nucleotides which are conserved, 154/657 nucleotides of varies, 124/657 parsimoni informative nucleotides and 30/657 singeleton nucleotides. The base N composition consist of 39.0% Timine, 14.0% Cytosin (C), 31.7%Adenine (A) and 15.4% Guanin.Key words: Plusia chalcites, COI Gene characteristics and composition. ABSTRAK Penelitian ini merupakan penelitian eksplorasi untuk mengkarakterisasi sekuen gen COI. Karakterisasi gen COI meliputi isolasi DNA menggunakan metode CTAB (Cetyl Trimetyl Ammonium Bromide), amplifikasi gen COI dengan teknik PCR (Polymerase Chain Reaction) dan squencing untuk analisis susunan basa nitrogen pada gen COI. Analisis data molekuler menggunakan software BioEdit dan MEGA Version 5.0.Hasil penelitian menunjukkan segmen gen COI Plusia chalcites  yang teramplifikasi berukuran 710 pb, dan terkarakterisasi sepanjang 657pb. Dari segmen tersebut terdapat 503/657 nukleotida yang bersifat conserved (kekal), 154/657 nukleotida yang bersifat variabel (bervariasi), 124/657 nukleotida parsimoni informative dan 30/657 nukleotida singleton. Komposisi basa nitrogen sekuen gen COI Plusia chalcites yaitu Timin (T) 39,0%, Cytosin (C) 14.0%, Adenin (A) 31,7% dan Guanin 15,4%.  .Kata kunci :Plusia chalcites, Karakteristik Gen COI
Pengolahan Ekoenzim dari Limbah Kulit Buah menjadi Pupuk Organik Cair dalam Mewujudkan Sektor Pertanian Rendah Karbon (Low-Carbon Agricultura) di Desa Konda Satu Kecamatan Konda Kabupaten Konawe Selatan Gusnawaty HS; Rachmi Hariaty Hasan; Resman Resman; Suriana Suriana
Jurnal Pengabdian Masyarakat (ABDIRA) Vol 6, No 3 (2026): Abdira
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/abdira.v6i3.2273

Abstract

The chemical dependency of farmers in Konda Satu Village and the accumulation of fruit peel waste potentially trigger greenhouse gas emissions. This Community Service (PKM) program aims to transfer science and technology in processing fruit peel waste into eco-enzyme as an environmentally friendly liquid organic fertilizer (POC). The implementation method was based on a participatory, educational, and applicative approach through socialization, direct technical guidance demonstrations, and field mentoring. The results of the activity showed a significant increase in community capacity, marked by more active participation in practicing eco-enzymes. This program also successfully compiled a practical guidebook and produced ready-to-use liquid fertilizer to stimulate a closed-loop agricultural system. In conclusion, this PKM activity has tangibly improved farmers' technical skills in processing waste into organic inputs toward low-carbon agriculture.
Co-Authors Amirullah Amirullah Astrid Indalifiany Bahari Bahari H.S., Gusnawaty Jamili Jamili Marwansyah Marwansyah Norma Arif Parakkasi Parakkasi Rachmi Hariaty Hasan Resman Resman Tjandra Buana