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Identification of excretory secretory (ES) liquid antigen protein Fasciola gigantica with polyethilene glycol (PEG) separation Yendri Junaidi; Ima Malawati; Made Sriasih
Indonesian Journal of Biotechnology Vol 21, No 2 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (824.988 KB) | DOI: 10.22146/ijbiotech.27017

Abstract

Fasciola gigantica diagnosis usually performed by detection of worm eggs presence in the feces,but this conventional method has many disadvantages. Early diagnosis (early detection) cannot be performed in conventional methods because the worms in the host's body began to lay eggs at the age of 8–12 weeks of patency. The current detection method that is based on antibody­antigen reactions using excreted/secreted (ES) liquid by adult F. gigantica, is believed to be used for the early detection of fasciolosis. This study aimed to characterize the antigenic components of F. gigantica extretory/secretory products that could be used as a vaccine candidate development for early fasciolosis diagnostics. ES products were separated by PEG4000 at various concentrations (8%, 16%, 24%), then precipitates (pellets) obtained were dialyzed and characterized using SDS­PAGE and Western blotting. Results from SDSPAGE showed that there were 18 proteins bands with 7–70 kDa molecular weights. Western blotting on pellets derived from PEG separation at various concentrations affirmed that the proteins of 50, 25 and 20 kDa were antigenic at 8% PEG concentration, the 25 kDa and 50 kDa were antigenic at 16% PEG concentrations and the 25 kDa was antigenic at 25% PEG concentration
Identifikasi Keragaman Jenis Parasit Cacing pada Ternak Ayam Kampung di Kabupaten Jember Satria Budi Kusuma; Suluh Nusantoro; Aan Awaludin; Yendri Junaidi; Tutik Lusyta Aulyani
Jurnal Ilmu Peternakan Terapan Vol 4 No 2 (2021): Jurnal Ilmu Peternakan Terapan
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Parasitic worms (Helminths) are the one of health problems related to the parasites investment that is often found in native chickens. This study aimed to identify the diversity of worm parasites found in native chickens in Jember Regency. The sampling procedure for native chicken excreta was carried out randomly. The excreta samples taken were 150 samples around Jember Regency. Identification of the diversity of worm species was carried out by examining worm eggs in excreta samples using the sedimentation method at BBVet (Balai Besar Veterinary) Wates, Yogyakarta. The parasitic worms identified in the excreta samples of native chickens were worms from the Nematode class consisting of Capillaria sp. (24.67%); Heterakis sp. (10.67%); Strongyloides sp. (3.33%); and Ascaridia sp. (2.67%).
Tradisi Manjujai dalam Pengembangan Bahasa Anak Usia Dini di Nagari Paninggahan Solok Lestari, Mega Cahya Dwi; Junaidi, Yendri; Yunita, Voni; Sartika, Diana; Wahyuni, Sri Intan
Raudhatul Athfal: Jurnal Pendidikan Islam Anak Usia Dini Vol. 7 No. 2 (2023): Raudhatul Athfal: Jurnal Pendidikan Islam Anak Usia Dini
Publisher : PIAUD Fakultas Ilmu Tarbiyah dan Keguruan Universitas Islam Negeri Raden Fatah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/ra.v7i2.16598

Abstract

Tujuan dari penelitian ini Untuk mendeskripsikan tradisi Manjujai di Minangkabau dalam pengembangan bahasa anak pada usia 1-24 bulan di Jorong Subarang, Nagari Paninggahan Kabupaten Solok. Hal ini dilatar belakangi oleh masih dilestarikannya budaya manjujai oleh orangtua khususnya Ibu sebagai bentuk pengasuhan di Jorong Subarang, Nagari Paninggahan, Kabupaten Solok. Jenis penelitian ini adalah penelitian fenomenologi yang merupakan jenis penelitian kualitatif dimana peneliti melihat dan mendengar lebih dekat dan terperinci penjelasan dan pemahaman individual tentang pengalaman-pengalamannya dalam melakukan studi terhadap kondisi yang alamiah. Adapun teknik pengumpulan data diambil melalui observasi, wawancara dan dokumentasi. Teknik analisis data dalam penelitian ini menggunakan pengumpulan data, reduksi data, penyajian data dan langkah terakhir adalah penarikan kesimpulan. Hasil penelitian menunjukkan bahwa tradisi manjujai memberikan dampak terhadap perkembangan bahasa anak dengan rentang usia 1-24 bulan. Intervensi yang diberikan dengan tujuan untuk berkomunikasi dengan anak, dan meningkatkan pemerolehan bahasa anak mulai dari kemampuan menyimak dan penambahan kosakata serta melatih kemampuan pelafalan anak dalam setiap huruf.
Implementation of Stimulation, Early Detection, and Intervention Programs for Monitoring the Growth and Development of Children Aged 2-3 Years Lestari, Mega Cahya Dwi; Ayu Citra Dewi; Sri Intan Wahyuni; Juliwis Kardi; Yendri Junaidi; Alif Laini
JURNAL PENDIDIKAN USIA DINI Vol 18 No 1 (2024): Jurnal Pendidikan Usia Dini Volume 18 Number 1 April 2024
Publisher : Program Studi Magister Pendidikan Anak Usia Dini

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/JPUD.181.13

Abstract

Starting from growth that supports development, in the end growth and development go hand in hand. An important period in a child's growth and development begins in infancy because basic growth is what will influence and determine the child's subsequent development. The aim of this research is to detect deviations in the development of early childhood children aged 2-3 years at the ECCE Rahmah El Yunusiyyah Padang Panjang through the Stimulation, Detection and Early Intervention of Child Growth and Development (SDICGD) instruments in the Android feature. This research was conducted using a cross-sectional approach. Participants in this study were 26 children aged 2-3 years using a consecutive sampling technique of 10 children whose growth and development were monitored using the SDICGD android application. The results of this study showed that several partisipants experienced malnutrition, one partisipant out of nine normal partisipants. The Developmental Pre-Screening Questionnaire (DPSQ) instrument of the ten child participants contained nine children (90%) who were according to the developmental stage aged 24-36 months, and one child was not according to the developmental stage. The results of the Attention Deficit and Hyperactivity Disorder (ADHD) test showed that three children (60%) were normal, and two children (40%) were hyperactive. Early detection of children's growth and development must be carried out regularly every month, and according to the child's age. For further research, it is hoped that early detection will also be carried out on the development of children's mental health, not just growth and development which is common and widely researched. Schools and parents must play an active role in children's growth and development so that no developmental stages are missed, and children grow and develop according to their age. 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Deteksi Dini Tumbuh Kembang pada Anak Usia Pra Sekolah. Jurnal Obsesi : Jurnal Pendidikan Anak Usia Dini, 7(4), 4682–4689. https://doi.org/10.31004/obsesi.v7i4.4517 Nursalam. (2014). Nursing Care for Infants and Children. Salemba Medika. Pandita, A., Sharma, D., Pandita, D., Pawar, S., Tariq, M., & Kaul, A. (2016). Childhood obesity: prevention is better than cure. Diabetes, metabolic syndrome, and obesity: targets and therapy, 9, 83–89. https://doi.org/10.2147/DMSO.S90783. Patel, A.B. et al. (2019) ‘M-SAKHI—Mobile health solutions to help community providers promote maternal and infant nutrition and health using a community-based cluster randomized controlled trial in rural India: A study protocol’, Maternal and Child Nutrition, 15(4), pp. 1–16. https://doi.org/10.1111/mcn.12850 Rahayu, S. F., Anggeriyane, E., & Mariani, M. (2021). Efforts to Strengthen Stimulation, Detection and Early Development and Development Program (SDIDTK) Through Anthropometric Examination in Preschool Children. Jurnal EMPATI (Edukasi Masyarakat, Pengabdian Dan Bakti), 2(1), 71. https://doi.org/10.26753/empati.v2i1.522 Roba, A.A. et al. (2021) ‘Prevalence and determinants of concurrent wasting and stunting and other indicators of malnutrition among children 6–59 months old in Kersa, Ethiopia’, Maternal and Child Nutrition, 17(3), pp. 1–12. https://doi.org/10.1111/mcn.13172. Sari, K. and Sartika, R.A.D. (2021) ‘The effect of the physical factors of parents and children on stunting at birth among newborns in Indonesia’, Journal of Preventive Medicine and Public Health, 54(5), pp. 309–316. https://doi.org/10.3961/jpmph.21.120. SDIDTK. (2016). Pedoman Pelaksanaan Stimulasi, Deteksi dan lntervensi Dini Tumbuh Kembang Anak. Direktorat Kesehatan Departmen Kesehatan Keluarga, 59. Shofiyati, et al. (2022). The Role of Teachers in Online Learning for Early Childhood Children in the Covid-19 Pandemic Era. Golden Generation: Journal of Ear-Ly Childhood Islamic Education., Vol. 5 No.https://journal.uir.ac.id/index.php/generationemas/article/view/8891 Shrestha, M.L. et al. (2022) ‘Malnutrition matters: Association of stunting and underweight with early childhood development indicators in Nepal’, Maternal and Child Nutrition, 18(2), pp. 1–9. https://doi.org/10.1111/mcn.13321. Soetjiningsih. (2014). Child Development. EGC. Suharsimi Arikunto. (2014). Prosedur Penelitian Suatu Pendekatan Praktik. Rineka Cipta. Suprayitno, E., Yasin, Z., Kurniati, D., & Rasyidah. (2021). Peran Keluarga Berhubungan dengan Tumbuh Kembang Anak Usia Pra Sekolah. Journal of Health Science, VI(II), 63–68. Tanuwijaya, S. (2014). General Concept of Growth and Development. EGC. Vanderloo, L.M. et al. 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PERBANDINGAN KUALITAS BIOGAS DARI BERBAGAI JENIS FESES TERNAK YANG DIPRODUKSI DENGAN DIGESTER PORTABLE Junaidi, Yendri; Hendrawati, Luki Amar; Riyanto, Riyanto
AGRIEKSTENSIA Vol 19 No 2 (2020): AGRIEKSTENSIA: Jurnal Penelitian Terapan Bidang Pertanian
Publisher : Politeknik Pembangunan Pertanian Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.955 KB) | DOI: 10.34145/agriekstensia.v19i2.943

Abstract

ABSTRAK Penggunaan energi oleh sektor transportasi yang mencapai lebih dari 30 persen dari total penggunaan energi nasional yang hampir seluruhnya (92%) bersumber dari bahan bakar minyak (BBM) selain masalah pada pasokan BBM juga berdampak buruk bagi lingkungan, sehingga harus ada upaya dalam mencari energi alternatif baru terbarukan, salah satunya adalahbiogas. Penelitian ini bertujuan untuk melihat kualitas biogas dari bebegai feses ternak seperti feses, sapi perah, sapi potong, kambing, domba, ayam broiler, ayam petelur dan feses keclinci. Pengamatan meliputi nilai pH dan temperatur harian, lama waktu pembentukan gas dalam masa fermentasi, lama produktivitas gas dari pembentukan gas pertama kali serta lama nyala api dalam dari hasi pembentukan gas harian. Biogas di produksi menggunakan digester portable dengan total waktu pengamatan selama 30 hari masa fermentasi dan produksi gas. Hasil Penetlitian menunjukan rata-rata nilai pH yang diapatkan dalam waktu 30 hari dari pH 6,2- pH 8. Temperatur rata-rata hasil pengamatan berada di angka 23-36 OC. Hasil uji ANOVA pada pada masing masing pengamatan yaitu pada lama pembentukan gas, lama produktifitas gas dan lama nyala api di dapatkan hasil yang signifikan yaitu f hitung <0,05. Hasil uji lanjut Tukey’S B pada masing-masing pengamatan di dapatkan hasil terbaik pada limbah feses sapi perah dengan data lama pembentukan gas (11,2 ± 1,3) hari, lama produktifitas gas (10,8± 1,9) dan lama nyala api adalah (20,6± 1,9) menit. Kata kunci: Feses, Biogas, alternatif, energi, kulitas ABSTRACT The use of energy by the transportation sector which reaches more than 30 percent of the total national energy use which is almost entirely (92%) comes from fuel oil (BBM) in addition to problems with fuel supply it also has a negative impact on the environment, so efforts must be made to find alternative energy. new renewables, one of which is biogas. This study aims to determine the quality of biogas from various animal feces such as feces, dairy cows, beef cattle, goats, sheep, broilers, laying hens and small animal feces. The observations included daily pH and temperature values, the length of time for gas formation during the fermentation period, the length of gas productivity from the first gas formation and the length of the internal flame from the daily gas formation. Biogas is produced using a portable digester with a total observation time of 30 days during fermentation and gas production. The results showed that the average pH value obtained within 30 days was from pH 6.2 to pH 8. The average temperature observed was 23-36 OC. The results of the ANOVA test on each observation, namely the duration of gas formation, the duration of gas productivity and the duration of the flame, obtained significant results, namely f count <0.05. Tukey'S B continued test results for each observation obtained the best results on dairy cow feces with data on the length of gas formation (11.2 ± 1.3) days, long gas productivity (10.8 ± 1.9) and duration of flame fire was (20.6 ± 1.9) minutes. Keywords: Feces, Biogas, alternatives, energy, quality
Utilization of Bacterial Consortium as a Biodisinfectan to Reduce Ammonia (NH3) Odor in Broiler Feces Yendri Junaidi; Suhirmanto Suhirmanto; Fitria Nur Aini
JURNAL TRITON Vol 16 No 1 (2025): JURNAL TRITON
Publisher : Politeknik Pembangunan Pertanian Manokwari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47687/jt.v16i1.1069

Abstract

Populasi ayam broiler tahun 2021 mencapai 3,11 miliar ekor, limbah feses mencapai 0,15 kg/ekor setiap hari Populasi broiler tahun 2021 mencapai 3,11 miliar ekor, limbah feses mencapai 0,15 kg/ekor setiap hari dengan potensi penghasil amonia (NH3) 54%. Untuk mengurangi konsentrasi ammonia (NH3) dibutuhkan biodisinfektan yang diproduksi dari konsorsium bakteri. Penelitian ini melakukan uji terhadap feses yang diberikan biodisinfektan mencakup total bakteri, konsentrasi amonia, nitrit, nitrat dan pH feses. Uji total bakteri menggunakan uji Total Plate Count (TPC), konsentrasi nitrit dan nitrat di uji mengacu SNI 06-2484-2004, uji pH menggunakan pH meter digital dan uji konsentrasi amonia (NH3) mengacu SNI 06-6989.30-2005. Analisis data menggunakan Analisis of Variance (ANOVA) taraf kepercayaan 95% dan uji lanjut Duncan menggunakan software SPSS 26.00. Hasil penelitian ini menunjukkan bahwa total bakteri biodisinfektan terbaik didapatkan pada perlakuan P3 (15% biodisinfektan). Kemudian Penurunan konsentrasi beberapa variabel pada feses perlakuan seperti nitrit yaitu 96,06%, nitrat yaitu 52,46%, amonia (NH3) yaitu dari 46 ppm menjadi 6 ppm atau penurunan sebesar 76,6% serta nilai pH terendah yaitu pH 4.7 juga di dapatkan pada perlakuan P3 (15% biodisinfektan). Penurunan konsentrasi amonia (NH3) sesuai dengan standar yang ditetapkan oleh Peraturan Menteri Lingkungan Hidup Indonesia Nomor 5 tahun 2014 yaitu 0,30 kg/ton atau setara dengan 30 ppm dan penurunan konsentrasi nitrit maupun nitrat sesuai dengan Peraturan Pemerintah Indonesia No. 82 tahun 2001.