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All Journal Jurnal Ilmiah Mahasiswa Veteriner Jurnal Medika Veterinaria
Erina Erina
Fakultas Kedokteran Hewan Universitas Syiah Kuala
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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ISOLASI Aspergillus sp PADA PARU-PARU AYAM KAMPUNG (Gallus domesticus) Isolation of Aspergillus sp from the Lung of Native Chicken (Gallus domesticus) nisma hayani; erina erina; darniati darniati
JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 4 (2017): AGUSTUS-OKTOBER
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (414.718 KB) | DOI: 10.21157/jim vet..v1i4.4489

Abstract

Penelitian ini bertujuan untuk mengisolasi Aspergillus sp pada paru-paru ayam kampung. Sampel yang digunakan dalam penelitian ini adalah 12 paru-paru ayam kampung yang diambil secara acak dari rumah potong unggas (RPU) Peunayong Banda Aceh. Isolasi Aspergillus sp dilakukan sesuai dengan metode Thompson (1969). Sampel dicuci dengan aquades steril yang berisi antibiotik selanjutnya ditanamkan pada media Sabouraud’s Dextrose Agar (SDA) kemudian diinkubasikan pada suhu kamar selama 2-7 hari. Pertumbuhan morfologi Aspergillus diamati secara makroskopis. Koloni yang diduga Aspergillus sp diperiksa secara mikroskopis. Data yang diperoleh dianalisis secara deskriptif. Hasil pemeriksaan menunjukkan bahwa Aspergillus sp dapat diisolasi pada 7 dari 12 sampel paru-paru. Dapat disimpulkan bahwa 58,33 % sampel paru-paru yang diperiksa positif terinfeksi Aspergillus sp dan 41,67 % bebas Aspergillus sp. This study aimed to isolate Aspergillus sp from the lungs of native chicken. Samples used in this study were 12 chicken lungs taken randomly from the poultry slaughterhouse Peunayong Banda Aceh. Isolation of Aspergillus sp were done based on Thompson method (1969). The samples were washed with sterile aquadest containing antibiotics before implanted on Sabouraud’s Dextrose Agar (SDA), then incubated at room temperature for 2-7 day. The plate was observed from Aspergillus sp colony macroscopically and microscopically. Data were analyzed descriptive. The result of examination showed that Aspergillus sp can be isolated 7 out of 12 lungs. It can be concluded that 58,33% of the lungs samples infected with Aspergillus sp and 41,67% were free from Aspergillus sp.
5. Antimicrobial Activity of Balakacida (Chromolaena odorata) Endophytic Bacteria Isolated from Aceh Besar Against Staphylococcus aureus and Pseudomonas aeruginosa Wahyu Eka Sari; Darmawi Darmawi; Morizka Wianda; Erina Erina; Rumi Sahara Zamzami; Muhammad Hambal; M. Nur Salim; Hennivanda Hennivanda; Triva Murtina Lubis
Jurnal Medika Veterinaria Vol 14, No 2 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i2.19415

Abstract

This study aims to isolate the balakacida (Chromolaena odorata) endophytic bacteria from Aceh Besar and to test its antimicrobial activity against pathogenic bacteria Staphylococcus aureus and Pseudomonas aeruginosa. Balakacida leaf samples were isolated and purified using nutrient agar (NA) media, followed by morphological observations and antimicrobial in vitro tests using a disk diffusion assay (Kirby Bauer). The results showed that a total of 50 endophytic bacterial colonies were isolated from the balakacida and 12 selected endophytic bacterial isolates had different morphologies from cream to yellowish cream color, then after antagonist test directly screening obtained four endophytic bacterial isolates which had the potential as antimicrobial. The results of in vitro antimicrobial tests showed that the four isolates of balakacida endophytic bacteria (BEA-1, BEA-2, BEA-3, and BEA-4) were able to inhibit the growth of pathogenic bacteria S. aureus and P. aeruginosa with the diameter of the zone of inhibition was 16.03 mm - 19.75 mm (medium inhibition category) and 15.35 mm - 21.24 mm (weak to strong inhibition category), respectively. Based on these results, it can be concluded that BEA-2 isolate has the strongest inhibition of 21.24 mm, so that it has the potential as an antimicrobial candidate for P. aeruginosa and can be used as an alternative antimicrobial product.
Co-Authors . Darniati Darmawi Darmawi Hennivanda Hennivanda M Nur Salim Morizka Wianda Muhammad Hambal nisma hayani Rumi Sahara Zamzami Sari, Wahyu Eka Triva Murtina Lubis