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Triva Murtina Lubis
Department Of Physiology, Faculty Of Veterinary Medicine, Syiah Kuala University, Banda Aceh
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Engineering Immunology & microbiology Medicine & Pharmacology Veterinary Other

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The traits of fresh and frozen semen on brahman bulls Triva Murtina Lubis; Prabowo Purwono Putro; Aris Junaidi; Sumarti Suryaningsih
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (104.983 KB)

Abstract

The present study aimed to determine quality and parameters traits of both fresh and frozen semen in 4-year old Brahman bulls. Semen was collected from 5 bulls twice a week using artificial vagina in Ungaran ArtificialInsemination Center, Central Java, Indonesia. Immediately after collection, samples were evaluated for semen volume, color, odor, viscosity, mass movement, individual movement, spermatozoa concentration and livespermatozoa percentage. Sperm concentration was measured using a spectrophotometer. Good quality fresh semen was processed to be frozen semen. Parameters evaluated for frozen semen include spermatozoa individualmovement, live spermatozoa, and abnormal spermatozoa percentage. The results showed that the semen volume is 6.90 ± 0.54 whereas the average of sperm concentration, individual motility and live sperm are 1754.54 ± 212.67;65.50 ± 3.74% and 76.30 ± 2.64%, respectively. Meanwhile, for frozen semen, the average percentage of motility is 38.00 ± 2.74% while the average proportions of live sperm and abnormal sperm are respectively 45.20 ± 9.86% and 6.00 ± 2.83%. In conclusion, in average, all parameters of fresh and frozen semen of Brahman bulls are in normal range. Based on the Indonesian National Standard, frozen semen produced could be used for insemination
THE POTENTIAL OF TOMATO EXTRACT (Lycopersicum esculentum L.) IN INCREASING THE SERTOLI CELLS AND SPERMATOGENIC CELLS COUNT OF THE CONTRALATERAL TESTICULAR SEMINIFEROUS TUBULES OF RATS (Rattus norvegicus) THAT HAVE TESTICULAR TORSION Dasrul Dasrul; Cut Nila Thasmi; Triva Murtina Lubis; Awaluddin Awaluddin; Winaruddin Winaruddin; Rasmaidar Rasmaidar; Yola Alifa
Jurnal Kedokteran Hewan Vol 15, No 3 (2021): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (631.529 KB) | DOI: 10.21157/j.ked.hewan.v15i3.20720

Abstract

The purpose of this study was to analyze the potential of tomato extract (Lycopersicum esculentum L.) in increasing the Sertoli and spermatogenic cells count of the contralateral testis in white rats (Rattus novergicus) with unilateral torsion. A total of 24 male Wistar white rats, aged 3-4 months old with a weight of 180-200 g were used in this study. The rat samples were randomly divided into 4 treatment groups: Group 1 as a negative control (NC) which consisted of rats without testicular torsion and given tomato extract; Group 2 as a positive control (PC) which consisted of rats with unilateral testicular torsion 720° for 6 hours without given tomato extract; Group 3 as treatment 1 (T1) consisted of rats that were subjected to unilateral testicular torsion 720o for 6 hours and were given tomato extract at a dose of 100 mg/kg BW/day; and Group 4 as treatment 2 (T2) consisted of rats that were subjected to unilateral testicular torsion 720° for 6 hours and were given tomato extract at a dose of 200 mg/kg BW/day. Each group consisted of six rats. Tomato extract was administered orally for 30 days. The observations of the number of Sertoli cells and spermatogenic cells (spermatogonia cells, spermatocytes, and spermatids) were carried out histologically with Hematoxylin-Eosin (HE) staining and the slides were examined with a light microscope. The data obtained was analyzed by ANOVA and continued with Duncan's test. The results showed that the administration of tomato extract significantly increased (P0.05) the number of Sertoli cells and spermatogenic cells of the contralateral testicular seminiferous tubule. Giving tomato extract 100 mg/kg BW/day resulted in an increase in the number of Sertoli cells and better spermatogenic cells than tomato extract 200 mg/kg BW/day. In conclusion the administration of tomato extract after detorsion can increase the number of Sertoli cells and spermatogenic cells of the contralateral testicular seminiferous tubules in white rats subjected to unilateral torsion of 720° for 6 hours
MEASUREMENT OF SERUM TESTOSTERONE IN KACANG GOAT BYUSING ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TECHNIQUE: THE IMPORTANCE OF KIT VALIDATION (Pengukuran Testosteron Serum Kambing Kacang dengan Teknik Enzyme-Linked Immunosorbent Assay (ELISA): Pentingnya Validasi Kit) Gholib G; Sri Wahyuni; Okta Hilda Kadar; Mulyadi Adam; Triva Murtina Lubis; Azhar A; Muslim Akmal; Tongku Nizwan Siregar; Teuku Armansyah; Taufiq Purna Nugraha
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (223.054 KB) | DOI: 10.21157/j.ked.hewan.v10i1.3367

Abstract

This study was conducted to validate a commercial testosterone enzyme-linked immunosorbent assay (ELISA) kits (DRG EIA-1559) inanalytic and biological manner for measuring serum testosterone concentrations in kacang goats. This study used 18 healthy kacang goats, six bucks (2 years), six kids (6 months), and six does (2 years). Blood samples were collected from jugular vein and prepared as serum. Two validation tests were performed, an analytical validation comprises a parallelism, accuracy, precision and sensitivity and a biological validation by comparing testosterone concentration from bucks, kids, and does. Testosterone concentrations were measured using ELISA technique. Data of analytical validation were analyzed descriptively and test of equality of slope was performed to see the parallelism between samples and standard curves. Analysis of variance (ANOVA) was used for biological validation data. Results of parallelism showed that sample curve was parallel to the standard curve. Accuracy, precision (% CV of intra-and inter-assay) and sensitivity of the assay were 99.65±4.27%, 10%, 15% and 0.083 ng/ml, respectively. Results of biological validation showed that the assay used were accurately measured testosterone which testosterone concentrations in bucks were significantly higher compared to kids and does (P0.05). In conclusion, a commercial testosterone ELISA kits (DRG EIA-1559) is a reliable assay for measuring serum testosterone concentration in kacang goats. Key words: analytical and biological validations, ELISA, testosterone, kacang goat
3. Efficacy of Jatropha (Jatropha curcas L) Cream sap Leucocytes in Inflammation Phase of Wound Healing Mita Nasuha Risky; Triva Murtina Lubis; M. Nur Salim; Teuku Zahrial Helmi; Abdul Harris; Hennivanda Hennivanda; Dian Masyitha; Cut Dahlia Iskandar; Fitriani Fitriani; Abdul Hamzah; T. Fadrial Karmil
Jurnal Medika Veterinaria Vol 14, No 2 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i2.19092

Abstract

This study aims to determine the efficacy of giving jatropha cream (Jatropha curcas L.) on the number of leukocytes in the inflammatory phase of wound healing in the mice’s skin. The method used is literature study research with qualitative techniques. The data used in this study comes from the result of studies that have been conducted and published in national or international online journals. References used are all research articles with data selection. The results of data analysis showed that the administration of jatropha cream (Jatropha curcas L.) was effective in limiting or inhibiting the accumulation of leukocytes in the area of inflammation and could stimulate the formation of new blood vessels (angiogenesis) in the inflammatory phase of wound healing of the mice’s skin. Based on these data it can be concluded that the efficacy of jatropha cream (Jatropha curcas L.) affects the number of leukocytes in the inflammatory phase of wound healing of mice's skin.
5. Antimicrobial Activity of Balakacida (Chromolaena odorata) Endophytic Bacteria Isolated from Aceh Besar Against Staphylococcus aureus and Pseudomonas aeruginosa Wahyu Eka Sari; Darmawi Darmawi; Morizka Wianda; Erina Erina; Rumi Sahara Zamzami; Muhammad Hambal; M. Nur Salim; Hennivanda Hennivanda; Triva Murtina Lubis
Jurnal Medika Veterinaria Vol 14, No 2 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i2.19415

Abstract

This study aims to isolate the balakacida (Chromolaena odorata) endophytic bacteria from Aceh Besar and to test its antimicrobial activity against pathogenic bacteria Staphylococcus aureus and Pseudomonas aeruginosa. Balakacida leaf samples were isolated and purified using nutrient agar (NA) media, followed by morphological observations and antimicrobial in vitro tests using a disk diffusion assay (Kirby Bauer). The results showed that a total of 50 endophytic bacterial colonies were isolated from the balakacida and 12 selected endophytic bacterial isolates had different morphologies from cream to yellowish cream color, then after antagonist test directly screening obtained four endophytic bacterial isolates which had the potential as antimicrobial. The results of in vitro antimicrobial tests showed that the four isolates of balakacida endophytic bacteria (BEA-1, BEA-2, BEA-3, and BEA-4) were able to inhibit the growth of pathogenic bacteria S. aureus and P. aeruginosa with the diameter of the zone of inhibition was 16.03 mm - 19.75 mm (medium inhibition category) and 15.35 mm - 21.24 mm (weak to strong inhibition category), respectively. Based on these results, it can be concluded that BEA-2 isolate has the strongest inhibition of 21.24 mm, so that it has the potential as an antimicrobial candidate for P. aeruginosa and can be used as an alternative antimicrobial product.
19. PENGARUH PEMBERIAN EKSTRAK TEH HIJAU (Camelia sinensis) TERHADAP PENURUNAN KADAR HEMOGLOBIN DAN NILAI HEMATOKRIT PADA TIKUS WISTAR (Rattus norvegicus) The Effect of Green Tea Extract (Camelia sinensis) on Haemoglobin and Hematocrit Levels of Wistar Rat (Rattus norvegicus) Triva Murtina Lubis
Jurnal Medika Veterinaria Vol 10, No 2 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v10i2.4624

Abstract

The aim of this study was to determine the effect of green tea (Camelia sinensis) extract on hemoglobin and hematocrit level of Wistar rat (Rattus norvegicus). Sample animals used were 20 rats strain Wistar aged 2.5-3.0 months. Green tea extract was given for 30 days consecutively. Blood collection was conducted via orbitalis sinus for 2 ml using capillary pipette. Rats were fed with standart commercial food (Turbo Feed T.79-4) and aquadest as drinking water ad libitum. This study used completely randomized design (CRD) pattern with 4 treatments and 5 replications. The control group (P0) without any treatment, P1 was given green tea extract 0.045 g/ml/day, P2 was given green tea extract 0.060 g/ml/day, and P3 was given green tea extract 0.075 g/ml/day. Data were analyzed by oneway analysis of variance (Anova). The result showed that mean±SD of hemoglobin levels (g/dl) on each treatment group (P0, P1, P2, and P3) were 11.86±0.55, 12.58±1.40, 11.82±0.69, and 12.50±0.84 respectivey. Mean±SD of hematocrit levels (%) on each treatment group (P0, P1, P2, and P3) were 44.00±4.30, 43.40±1.14, 44.00±1.58, and 42.80±1.79. To conclude, the administration of green tea extract has no effect (P0.05) on haemoglobin and hematocrit levels of Wistar rat. ____________________________________________________________________________________________________________________ Key words: green tea, haemoglobin, haematocrit, Wistar rat
10. The Sperm Quality of Diabetic Rat (Rattus norvegicus) After Treated with Bitter Melon (Momordica charantia L.) Extract Elsa Suarni; Dasrul Dasrul; Triva Murtina Lubis; Azhar Azhar; Rinidar Rinidar; Ismail Ismail
Jurnal Medika Veterinaria Vol 14, No 2 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i2.3679

Abstract

The purpose of this study was to determine the sperm quality of  diabetic  rat (Rattus norvegicus) after treated with bitter melon (Momordica charantia L.) extracts. This study followed direct complete randomized design using 15 male rats (Rattus norvegicus) Wistar’s strain which divided into 3 groups. group I (K1) as a control, group II (K2) was diabetic rat  which induced 120mg / kg bw alloxan intraperitoneally, and group III (K3) was diabetic rat which treated with 100mg / kg bw extract of bitter melon, Examination of sperm count, motility, and sperm abnormality was checked at day 48. Data sperm count, motility, and sperm abnormalities was analyzed using ANOVA and Duncan multiple test. The results showed that administration of bitter melon extract dose  100 mg / kg bw for 48 days can improve count and motility spermatozoa (P0,05) and decrease abnormalities of spermatozoa (P0,05).   Bitter melon fruit extract dose  100 mg / kg bw can prevent decrease  sperm quality of diabetic rats
Efficacy of Jatropha (Jatropha curcas L.) Sap Cream on Total Differential Leucocytes in Inflammatory Phase of Wound Healing on Mice Skin (Mus musculus) : A Review Aufira Fildza Bahittah; M. Nur Salim; Triva Murtina Lubis; T. Zahrial Helmi; Abdul Harris; Wahyu Eka Sari
Jurnal Medika Veterinaria Vol 17, No 1 (2023): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v17i1.23542

Abstract

A wound is a damage to the integrity of the epithelium of the skin. There are three phases of wound healing process, the first phase is inflammatory phase, the second phase is fibroplasia phase, and the third is maturation phase. Inflammation that occurs during the wound healing process should be limited because it would cause the wound to undergo an abnormal healing process and pathological inflammation if it persists. The aim of this study was to determine the efficacy of jatropha (Jatropha curcas L.) sap cream on total differential leucocytes in inflammatory phase of wound healing on mice skin. The method used was literature study using qualitatSive methods. Data used in this study refers to the result of studies which have been conducted and published in national and international online journal. Result of data analysis showed that Jatropha sap cream was effectively limiting and  inhibiting the leucocytes accumulation in inflammatory area and stimulated new blood vessels formation (angiogenesis) in inflammatory phase of wound healing in mice skin. Based on the result of this study, it can be concluded that the Jatropha sap cream could reduce the number of differential leucocytes in inflammatory phase of wound healing on mice skin.
Perception of Cat Owners about the Management of Cat Care During Pregnancy and Post Partus Period Zarizki Taqwa; Triva Murtina Lubis; Dasrul Dasrul
Jurnal Medika Veterinaria Vol 16, No 2 (2022): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v16i2.11903

Abstract

Cats are pets which part or all of their lives depend on humans for certain things. The period of pregnancy and post partus are very crucial periods for cats. It required certain management approach which is different from general maintenance. This study aimed to find out the perception of cat owners about the cat's management during the pregnancy and post partus period. The research data were obtained by filling out the online questionnaires distributed to the member of cat lover’s Banda Aceh. Data were analyzed descriptively. This study found that the perception of cat owners during pregnancy and post partus was positive.
Determination of Cadmium Accumulation in Livers and Feces of Kacang Goats Grazing Tsunami Affected Land Cut Dahlia Iskandar; Triva Murtina Lubis
Jurnal Agripet Vol 10, No 1 (2010): Volume 10, No. 1, April 2010
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v10i1.631

Abstract

Penentuan akumulasi cadmium (Cd) dalam hati kambing kacang di daerah tsunamiABSTRAK. Tujuan dari penelitian ini adalah untuk mendeteksi akumulasi cadmium pada hati dan feces kambing Kacang dan sekaligus mengukur kadar akumulasi cadmium tersebut. Limabelas ekor kambing Kacang yang berasal dari rumah potong di daerah Tsunami dan limbelas ekor kambing Kacang yang berasal dari rumah potong di daerah Non-Tsunami. Diikuti dengan lima ekor kambing Kacang dari desa di daerah Tsunami dan lima ekor kambing Kacang dari desa di daerah Non-Tsunami. Ke-empatpuluh ekor kambing Kacang tersebut disembelih untuk diambil hati dan feces. Sebelum hati dan feces tersebut akan dipreparasi untuk analisis akumulasi dari cadmium, dilakukan pengamatan anatomi dari hati dan feces tersebut. Cadmium berakumulasi dihati dan feces dari kambing Kacang, baik yang berasal dari daerah Tsunami dan dari daerah Non-Tsunami. Akumulasi cadmium didalam hati kambing Kacang signifikan lebih tinggi di daerah Non-Tsunami dibandingkan didalam hati kambing Kacang di daerah Tsunami. Pada feces kambing Kacang akumulasi cadmium juga signifikan lebih tinggi di daerah Non-Tsunami dibandingkan didalam feces kambing Kacang di daerah Tsunami.
Co-Authors Abdul Hamzah Abdul Harris Abdul Harris Aris Junaidi Aufira Fildza Bahittah Awaluddin Awaluddin Azhar A Azhar Azhar Cut Dahlia Iskandar Cut Nila Thasmi Darmawi Darmawi Dasrul Dasrul Dasrul Dasrul Dian Masyitha Elsa Suarni Erina Erina Fauziah Fauziah Fitriani Fitriani Gholib Gholib Hamdan Hamdan Hennivanda Hennivanda Ismail Ismail M Nur Salim M. Hasan M. Nur Salim Mita Nasuha Risky Morizka Wianda Muhammad Hambal Mulyadi Adam Muslim Akmal Nazaruddin N Nuzul Asmilia Okta Hilda Kadar Prabowo Purwono Putro Rasmaidar Rasmaidar Rinidar Rinidar Rumi Sahara Zamzami Sari, Wahyu Eka Siti Aisyah Sri Wahyuni Sumarti Suryaningsih T. Fadrial Karmil T. Zahrial Helmi Taufiq Purna Nugraha Teuku Armansyah Teuku Zahrial Helmi Tongku Nizwan Siregar Winaruddin Winaruddin Yola Alifa Yudha Fahrimal Zarizki Taqwa