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All Journal ACTA VETERINARIA INDONESIANA Jurnal Ilmiah Mahasiswa Veteriner Jurnal Pendidikan Dasar Albacore : Jurnal Penelitian Perikanan Laut Jurnal Natural The International Journal of Tropical Veterinary and Biomedical Research Jurnal Medika Veterinaria
Sari, Wahyu Eka
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Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Energy Immunology & microbiology Medicine & Pharmacology Neuroscience Physics Public Health Veterinary

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PENERAPAN LEMBAR KERJA SISWA (LKS) TERHADAP PEMAHAMAN KONSEP BANGUN DATAR PADA SISWA KELAS II SDN 7 KEBEBU sari, wahyu eka; Waridah, Waridah; Sukardi, Sukardi
Jurnal Pendidikan Dasar Vol 7, No 1 (2019): EDISI JUNI 2019
Publisher : STKIP Melawi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (47.24 KB)

Abstract

Abstract: The purpose of this research to determine differences in understanding two-dimensional geometry concepts of students before and after application of worksheet in second grade students of SDN 7 Kebebu. This research is an pre-experimental research and used one group pretest-posttest design. Data collection techniques used essay tests. The Worksheet and instrument is validated by two experts namelly material expert and linguists used content validity. To determine reliability instrument data was analyzed used Cronbach Alpha formula, liliefors test for normality of data and t test to determine differences in understanding two-dimensional geometry concepts before and after used worksheet. The result of calculation used the t test showed that the sig value of 0,000 < 0,05 so that Ha is received. It can be conclude that there are significant difference in understanding two-dimensional geometry concept between before and after application of worksheet in second grade students of Elementary School 7 Kebebu.Keywords: worksheet, understanding two-dimensional geometry concept Abstrak: Penelitian ini bertujuan untuk mengetahui perbedaan pemahaman konsep bangun datar siswa sebelum dan sesudah penerapan Lembar Kerja Siswa (LKS). Penelitian ini merupakan penelitian pre-eksperimen dan menggunakan desain one group pretest-posttest. Teknik pengumpulan data menggunakan tes tertulis esai. Lembar kerja siswa (LKS) dan instrumen penelitian divalidasi oleh dua ahli yaitu ahli materi dan ahli bahasa menggunakan validitas isi. Untuk mengetahui reliabilitas instrumen data dianalisis dengan menggunakan rumus Alpha Cronbach, uji liliefors untuk kenormalan suatu data dan uji-t untuk mengetahui perbedaan pemahaman konsep bangun datar sebelum dan sesudah menggunakan lembar kerja siswa (LKS). Hasil perhitungan menggunakan uji-t menunjukkan bahwa nilai sig 0,000 < 0,05 sehingga Ha diterima. Maka dapat disimpulkan bahwa terdapat perbedaan pemahaman konsep bangun datar yang signifikan antara sebelum dan sesudah penerapan lembar kerja siswa (LKS) di kelas II Sekolah Dasar Negeri 7 Kebebu.Kata Kunci: Lembar kerja siswa (LKS), pemahaman konsep bangun datar
ANGKA PREVALENSI CEMARAN BAKTERI Escherichia coli PADA DAGING AYAM BROILER YANG DIJUAL DI TIGA PASAR TRADISIONAL KOTA BANDA ACEH (The Prevalence Rate of Escherichia coli Contamination in Broiler Chicken Meat Sold in Three Traditional Markets in Banda Aceh) erma yusintha ramdhania; T Reza Ferasyi; wahyu eka sari; mahdi abrar; ismail ismail ismail; cut nila thasmi
JURNAL ILMIAH MAHASISWA VETERINER Vol 4, No 3 (2020): MEI-JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v4i3.15180

Abstract

ABSTRAK Escherichia coli merupakan salah satu bakteri penyebab foodborne disease yang dapat menimbulkan penyakit berbahaya apabila bahan pangan yang terkontaminasi dikonsumsi oleh manusia. Salah satu bahan pangan asal hewan yang sering dikonsumsi masyarakat adalah daging ayam, baik daging ayam kampung maupun daging ayam broiler. Penelitian ini bertujuan untuk mengetahui angka prevalensi bakteri E. coli pada daging ayam broiler yang dijual di Tiga Pasar Tradisional Kota Banda Aceh. Sampel yang digunakan dalam penelitian ini adalah 25 sampel daging ayam broiler yang diambil dari Tiga Pasar Tradisional. Penelitian ini menggunakan metode total plate count dengan larutan buffered peptone water (BPW) dan media selektif differensial eosin methylene blue Agar (EMBA). Hasil penelitian menunjukkan bahwa sembilan sampel daging ayam broiler positif tercemar E.  coli dengan angka prevalensi yaitu di Pasar Seutui (100%) dan Pasar Peunayong (26,7%). Dapat disimpulkan bahwa cemaran bakteri E. coli melebihi batas normal dengan angka prevalensi secara keseluruhan dari Tiga Pasar Tradisional di Kota Banda Aceh yaitu sebesar 36%.  Kata kunci : Escherichia coli, daging ayam broiler, foodborne disease, prevalensi ABSTRACT Escherichia coli is one of the bacteria that cause foodborne disease which can cause dangerous diseases if contaminated food is consumed by humans. One foodstuff of animal origin that is often consumed by the public is chicken meat, both native chicken meat and broiler chicken meat. This study aims to determine the prevalence of E. coli in broiler chicken meat sold in the Three Traditional Markets in Banda Aceh. The sample used in this study was 25 samples of broiler chicken meat taken from the Three Traditional Markets. This study uses the total plate count method with buffered peptone water (BPW) and differential selective media eosin methylene blue agar (EMBA). The results showed that nine samples of broiler’s chicken meat were positively contaminated E. coli with prevalence rates in Seutui Market (100%) and Peunayong Market (26.7%). It can be concluded that the contamination of E. coli exceeds normal limits with an overall prevalence rate of the Three Traditional Markets in Banda Aceh City is 36%. Keywords : Escherichia coli, broiler chicken meat, foodborne disease, prevalence  
PENENTUAN DAERAH PENANGKAPAN MADIDIHANG (Thunnus albacares) DI PERAIRAN PROVINSI ACEH BERDASARKAN KOMPOSISI UKURAN PANJANG Samsul Bahri; Wahyu Eka Sari
ALBACORE Jurnal Penelitian Perikanan Laut Vol. 2 No. 3 (2018): Albacore
Publisher : Departemen PSP IPB

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (560.017 KB) | DOI: 10.29244/core.2.3.307-314

Abstract

Tuna madidihang (Thunnus albacares) merupakan jenis tuna yang memiliki nilai ekonomis penting, dan termasuk ke dalam kelompok jenis pelagis besar, dengan siklus hidupnya berada di perairan tropis dan sub-tropis. Madidihang beruaya pada lintas perairan untuk mencari sumber makanan dan melakukan pemijahan. Populasi madidihang di perairan terancam akibat terjadinya degradasi daerah penangkapan. Hal ini disebabkan oleh penangkapan yang tidak terkontrol, sehingga mengakibatkan jumlah populasi madidihang yang terdapat di perairan Provinsi Aceh semakin mengecil pada ukuran panjang. Upaya dalam menjaga potensi madidihang di perairan Provinsi Aceh dengan menghitung komposisi ukuran hasil tangkapan berdasarkan waktu (temporal) dan daerah penangkapan (spasial), hal ini dapat menjadi langkah dalam menekan tingkat pemanfaatan sumberdaya madidihang belum layak tangkap secara besar-besaran. Metode dalam penelitian meliputi pengumpulan data primer dan data sekunder, serta análisis hasil tangkapan berdasarkan komposisi ukuran panjang untuk menentukan daerah penangkapan madidihang. Tujuan mengetahui komposisi ukuran panjang hasil tangkapan berdasarkan lokasi dan waktu penangkapan, agar dapat menentukan jenis alat tangkap yang dioperasikan untuk menghindari tertangkapnya ikan-ikan madidihang belum layak tangkap (ilegal size). Penentuan daerah penangkapan (fishing ground) madidihang secara spasial dan temporal dapat diinterpretasikan dalam peta daerah penangkapan. Optimasi penangkapan madidihang lebih efektif di perairan Pulau Aceh, Samudera Hindia, dan perairan Pulau Rondo.Kata kunci: Daerah penangkapan ikan, Provinsi Aceh, , Thunnus albacares, tuna Madidihang
5. Antimicrobial Activity of Balakacida (Chromolaena odorata) Endophytic Bacteria Isolated from Aceh Besar Against Staphylococcus aureus and Pseudomonas aeruginosa Wahyu Eka Sari; Darmawi Darmawi; Morizka Wianda; Erina Erina; Rumi Sahara Zamzami; Muhammad Hambal; M. Nur Salim; Hennivanda Hennivanda; Triva Murtina Lubis
Jurnal Medika Veterinaria Vol 14, No 2 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i2.19415

Abstract

This study aims to isolate the balakacida (Chromolaena odorata) endophytic bacteria from Aceh Besar and to test its antimicrobial activity against pathogenic bacteria Staphylococcus aureus and Pseudomonas aeruginosa. Balakacida leaf samples were isolated and purified using nutrient agar (NA) media, followed by morphological observations and antimicrobial in vitro tests using a disk diffusion assay (Kirby Bauer). The results showed that a total of 50 endophytic bacterial colonies were isolated from the balakacida and 12 selected endophytic bacterial isolates had different morphologies from cream to yellowish cream color, then after antagonist test directly screening obtained four endophytic bacterial isolates which had the potential as antimicrobial. The results of in vitro antimicrobial tests showed that the four isolates of balakacida endophytic bacteria (BEA-1, BEA-2, BEA-3, and BEA-4) were able to inhibit the growth of pathogenic bacteria S. aureus and P. aeruginosa with the diameter of the zone of inhibition was 16.03 mm - 19.75 mm (medium inhibition category) and 15.35 mm - 21.24 mm (weak to strong inhibition category), respectively. Based on these results, it can be concluded that BEA-2 isolate has the strongest inhibition of 21.24 mm, so that it has the potential as an antimicrobial candidate for P. aeruginosa and can be used as an alternative antimicrobial product.
6.Detection of Escherichia coli Contamination in Vaname Shrimp (Litopenaues vannamei) at Fish Auction Place (TPI) Lampulo Banda Aceh Wilyo Hendro Fauzan; Wahyu Eka Sari; Andi Novita; Faisal Jamin; Razali Daud5; Rastina Rastina; Safika Safika
Jurnal Medika Veterinaria Vol 15, No 2 (2021): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v15i2.26075

Abstract

Shrimp is an easily polluted material, so it requires good handling. Contamination can occur during harvest, handling, or transportation. This study aims to detect Escherichia coli contamination in vaname shrimp sold at TPI Lampulo Banda Aceh. A total of 10 samples of vaname shrimp were obtained from 10 merchants at TPI Lampulo. The research method used is observational, and the data analysis is done descriptively. The detection of E. coli contamination was carried out by the total plate count (TPC) test method. Based on the study results, two of the 10 samples of vaname shrimp merchant detected E. coli bacterial contamination, namely the results of sample 01 merchant indicated the number of TPC 2x102 CFU/g and in 09 merchants indicated the number of TPC 11x101 CFU/g. The TPC results were indicated by the growth of E. coli colonies on eosin methylene blue agar (EMBA) media after incubation for 24 hours at 37 ºC. The number of the two detected positive samples showed less than 1x103 CFU/g by the provisions of SNI 2332.3:2015. Thus, it can be concluded that E. coli contamination was detected in vaname shrimp sold by the merchant at TPI Lampulo but did not exceed the SNI threshold, so it was safe for consumption.
Nigella Sativa Seed Extract has Potential Antimicrobial Activity on Pasteurella multocida in Vitro Vanda, Henni; Zakya Ardi; Sari, Wahyu Eka; Hambal, Muhammad
Acta VETERINARIA Indonesiana Vol. 11 No. 3 (2023): November 2023
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.11.3.228-233

Abstract

Nigella sativa known as black cumin has various bioactive compounds to treat many diseases. Some of the benefits of N. sativa seed are as immune booster, antihistamin, anti-diabetic, antihypertension, antiinflammatory, antimicrobial, and antitumor. This study aimed at determining the antimicrobial activity and finding out the effective concentration of N. sativa seed ethanolic extract on Pasteurella multocida in vitro. N. sativa seed extract was divided into three groups consisting of 15% extract (T1), 25% extract (T2), 45% extract (T3), and chloramphenicol was used as positive control. For antimicrobial test, Kirby Bauer diffusion method was used, and the data were analyzed by ANOVA. The results revealed that T3 had the most effective antimicrobial activity, shown by the largest inhibition zone (17.72 mm), followed by T2 (15.93 mm) and T1 (10.75 mm). ANOVA test results showed significant differences (P<0.05) in each group. The antimicrobial effect of T3 and T2 was categorized as strong, whilst T1 as moderate. From the results it can be concluded that N. sativa seed extract had strong and moderate inhibition activity on the growth of P. multocida, therefore, N. sativa seed is a potential candidate for antimicrobial drug development againts P. multocida.
Multi-drug resistance-1 (MDR-1) gene expression in MCF-7 cells after treated with doxorubicin-deoxyelephantopin combination and prediction of inhibitory activity against Pgp receptors with in silico DANIEL, DANIEL; FRENGKI, FRENGKI; JALALUDDIN, MUHAMMAD; SARI, WAHYU EKA; ROSMAIDAR, ROSMAIDAR; HASRIATI, HASRIATI; ZAKIAH, NONI
Jurnal Natural Volume 24 Number 3, October 2024
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v24i3.33237

Abstract

Doxorubicin chemotherapy has been a strong focus in breast cancer research. Side effects, toxicity and resistance have been extensively studied. One proposed solution to these issues that its combination with deoxyelephantopin. Deoxyelephantopin is known to be toxic in many cancer cells but safe in normal cells. IC50 of each compound were determined by using a MTT assay, and the MDR-1 gene mRNA expression were determined by using qPCR method, while the interaction of doxorubicin in combination with deoxyelephantonin on Pgp receptor were predicted by using an in silico approach. The IC50 of deoxyelephantopin was found to be 11.2 g/mL, while IC50 of doxorubicin was 448 nM IC50 values showed a deoxyelephantopin-doxorubicin combination was able to reduce MDR-1 expression by 19% compared to doxorubicin and IC50 values indicated that the combination formula reduced the expression by 15% over doxorubicin alone. The affinity of doxorubicin and deoxyelephantopin is -12.16 kcal/mol and -9.51 kcal/mol, respectively, while the affinity of doxorubicin after combine with deoxyelephantopin decreases from -12,16 kcal/mol to -11,25 kcal/mol due to the release of one Leu 221 hydrogen bond. The combination of doxorubicin with deoxyelephantopin is able to reduce expression and suppress the function of the Pgp protein.
Screening of Pathogenic and Non-Pathogenic Bacteria Isolated from Maggot Digestive System Azhari, Azhari; Sari, Wahyu Eka; Ferasyi, Teuku Reza; Ismail, Ismail; Novita, Andi; Darniati, Darniati; Riady, Ginta; Fadlah, Iga; Rahmadhini, Vivi
The International Journal of Tropical Veterinary and Biomedical Research Vol 9, No 2 (2024): Vol. 9 (2) November 2024
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/ijtvbr.v9i2.44058

Abstract

Maggots are one of the fly larvae with a high protein content of up to 30-45%, so maggots can be used as an alternative animal feed ingredient. As one of the sources of animal feed raw materials, insects-based feed must also be safe from contaminants of microorganisms, including pathogenic bacteria. Therefore, this study aims to isolate and determine the screening of pathogenic and non-pathogenic bacteria from maggots' digestive systems. Maggots are cultivated on media based on animal faecal and organic waste for 2 months. Then, the bacteria are isolated and purified using nutrient agar media, and hemolysis tests are carried out on blood agar media and Gram staining. Eighty-seven bacterial colonies with various morphological characteristics were successfully isolated from the maggot digestive system. Most isolated bacteria are classified as Gram-negative bacteria with a bacilli form. Based on the results of the hemolysis test, as many as 16% of bacterial isolates are indicated by pathogenic bacteria because of their ability to hemolyze blood. However, only about 2% showed b-hemolysis. Thus, it can be concluded that the screening results of non-pathogenic bacteria are still more numerous compared to pathogenic bacteria present in the maggot digestive system.
Prevalence of Cases of Feline Lower Urinary Tract Disease (FLUTD) in Patients Treated at Veterinary Teaching Hospital During 2022 Zamzami, Rumi Sahara; Panjaitan, Budianto; Yuliansyah, Nanda; Ferdian, Riyan; Sari, Wahyu Eka; Nurliana, Nurliana; Awaluddin, Awaluddin; Rizki, Maurina; Hasyimi, Adyar
The International Journal of Tropical Veterinary and Biomedical Research Vol 7, No 2 (2022): Vol. 7 (2) November 2022
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/ijtvbr.v7i2.30505

Abstract

Feline lower urinary tract disease (FLUTD) is a complex patient condition caused by many factors. This case is the reason why pet owners always need veterinary assistance. Cats with urinary tract disorders show almost similar symptoms such as pollakiuria, hematuria, stranguria, urethral obstruction, and even urination outside the litter box. Urethral obstruction is a common complication in cases of FUS, which often occurs in male cats. Based on this, this study was conducted to determine the prevalence rate of FUS in the cat patient population at RSHP in 2022. The total number of patients treated at Veterinary Teaching Hospital (VTH) Prof. Dr. Noerjanto Faculty of Veterinary Medicine, Universitas Syiah Kuala was 849, and 38 cases of cat patients experiencied FLUTD. This research is an observational study to see the prevalence rate of FLUTD in a population of cat patients treated at the VTH. Based on the research that has been done, the prevalence value of animals experiencing FLUTD is 4.47. The number of cases of FLUTD in male cats is (97.3%) and for female cats (2.63%). The incidence of FLUTD that occurred in cats aged 2 to 4 years old.
Detection of Pathogenic Escherichia coli in Duck Intestine Slaughtered at Lambaro Market Aceh Besar Erina, Erina; Panjaitan, Tasya Adilla; AK, M. Daud; Sari, Wahyu Eka; Rusli, Rusli; Helmi, Teuku Zahrial
Jurnal Medika Veterinaria Vol 19, No 1 (2025): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v19i1.44883

Abstract

Pathogenic Escherichia coli is a type of bacteria that has the ability to cause disease in hosts, including humans and animals, although in general E. coli acts as a normal flora in the digestive tract. This study aimed to detect the presence of pathogenic E. coli in the intestines of ducks sold at Lambaro Market, Aceh Besar. E. coli is a normal flora bacteria in the digestive tract of animals and humans, but some strains are pathogenic and can cause zoonotic diseases. The presence of pathogenic E. coli in poultry products has the potential to be a source of infection for humans through consumption of contaminated food. This study used 15 duck intestine samples obtained from cecum, to identify the presence of pathogenic E.coli. The results showed that 13 samples produced clear colonies on Sorbitol MacConky Agar (SMAC), indicating the inability of bacteria to ferment sorbitol, which correlated to the presence of pathogenic E. coli. In contrast, two samples showed pink colonies indicating the presence of nonpathogenic E. coli. The conclusion of this study was the cecum of ducks sold at Lambaro Market, Aceh Besar positive for pathogenic E. coli.
Co-Authors . Darniati Andi Novita Awaluddin Awaluddin Azhari Azhari budianto panjaitan cut nila thasmi Daniel Daniel Darmawi Darmawi Erina Erina Erina Erina erma yusintha ramdhania Erwin Erwin Etriwati E Fadlah, Iga Faisal Jamin Ferdian, Riyan Frengki Frengki, Frengki Ginta Riady Hasriati Hasriati, Hasriati Hasyimi, Adyar Henni Vanda Hennivanda Hennivanda Hilmi, Mulkiah Ismail Ismail ismail ismail ismail Jessica Anggun Safitri M Daud AK M Nur Salim Mahdi Abrar Morizka Wianda Muhammad Hambal Muhammad Hambal Muhammad Jalaluddin, Muhammad Muttaqien Muttaqien, Muttaqien Nanda Yulian Syah Noni Zakiah NURLIANA NURLIANA Panjaitan, Tasya Adilla R. Rusli Rahmadhini, Vivi Rahmi Eka Putri Rahmi, Ulfa Rastina Rastina Razali Daud5 Rizki, Maurina Rosmaidar Rosmaidar, Rosmaidar Rumi Sahara Zamzami Safika S, Safika Samsul Bahri Sugito - Sukardi Sukardi Syah, Nada Yulian T Reza Ferasyi Teuku Reza Ferasyi Teuku Zahrial Helmi Triva Murtina Lubis Waridah Waridah, Waridah Wilyo Hendro Fauzan Zakya Ardi Zamzami, Rumi Sahara Zulfayanto, Indri