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Analisis Hambatan Pembelajaran Biologi dalam Implementasi Kurikulum Merdeka di SMA Katolik St. Rosa De Lima Tondano Gea, Aguslina; Semuel, Mokosuli Yeremia; Posumah, Dany Christian; N. Tanor, Meity; P. Gedoan, Sukmarayu; R. Kawuwung, Femmy
Jurnal Inovasi dan Teknologi Pendidikan Vol. 4 No. 2 (2025): Jurnal Inovasi dan Teknologi Pendidikan
Publisher : LPPM Universitas Bina Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46306/jurinotep.v4i2.206

Abstract

This study aims to analyze the obstacles faced by biology teachers and students in the implementation of the Independent Curriculum at St. Rosa De Lima Catholic High School in Tondano. The Merdeka Curriculum emphasizes freedom of thought, project-based learning, and the development of critical and collaborative thinking skills, but its implementation still faces obstacles at the secondary school level. This study uses a descriptive qualitative approach with data collection techniques through observation, questionnaires, interviews, and documentation. The research subjects consisted of one biology teacher and ten students of class X. Data were analyzed descriptively, qualitatively, and quantitatively with percentage support to describe the level of resistance based on four main indicators: learning process, teaching materials, assessment, and curriculum information. The results of the study show that teachers' obstacles in the implementation of the Independent Curriculum are relatively low with an average of 18.6%, especially in terms of time management, limited laboratory facilities, and limited digital learning resources. In contrast, internal student factors such as interest, motivation, readiness, and attention had an average of 67.17%, while external factors included family, school environment, and community support at 55.5%. These findings indicate that the success of the implementation of the Independent Curriculum depends on the pedagogical readiness of teachers, the availability of learning facilities, and the support of a conducive learning environment. This research contributes to providing an empirical picture of the challenges of implementing the Independent Curriculum in biology learning and becomes the basis for the development of adaptive, collaborative, and student-centered learning strategies.
Designing Practical Guides Based on Simple Tools and Materials to Improve Students' Understanding in Scientific Learning at School Tampi, Grichelle; Nanlohy, Fanny Nella; Posumah, Dany
Jurnal Ilmiah Mandala Education (JIME) Vol 10, No 2 (2024): Jurnal Ilmiah Mandala Educartion (April)
Publisher : Lembaga Penelitian dan Pendidikan Mandala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58258/jime.v10i2.6851

Abstract

Scientific learning plays an important role in the development of students' understanding of concepts and skills in science. Although there are often obstacles in providing practical experience to students, this triggers teacher creativity and innovation to overcome the limitations of tools and materials at school. This study aims to design a practicum guide based on simple tools and materials that are feasible to use to improve student understanding in scientific learning at school. The research method used in this study is the development research method according to Borg and Gall (1983) modified by Rengkuan and Howan (2016), into five development steps which include: (1) Need Analysis & Reference Study, (2) Material Development, (3) Expert Validation, (4) Try Out, (5) Final Production. The results showed the feasibility of designing a practicum guide based on simple tools and materials obtained from material experts with a percentage of 93.3% with a very feasible category, from media experts with a percentage of 90.3% with a very feasible category, from student practicality assessments with a percentage of 84.3% with a very feasible category, and from teacher responses with a percentage of 90.3% with a very feasible category. So it can be concluded that the design of practicum guides based on simple tools and materials is very feasible to use to improve student understanding in scientific learning at school. <
Lactic Acid Bacteria That Produce Antibacterial Compunds on Candied Pakoba Fruit Syzygium luzonense Runtuwene, Kharly; Lawalata, Helen; Posumah, Dany; Satiman, Utari; Moko, Emma
Indonesian Biodiversity Journal Vol. 6 No. 2 (2025): August 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/srqbqw88

Abstract

Lactic Acid Bacteria (LAB) are a group of Gram-positive, cocci or rod-shaped microorganisms characterized by their catalase-negative phenotype. These bacteria are known to produce various antibacterial compounds, including organic acids, hydrogen peroxide, carbon dioxide, and bacteriocins, which exhibit bacteriostatic or bactericidal properties against pathogenic bacteria. The aim of this study is to isolate LAB that produce antibacterial compounds during the fermentation of Pakoba fruit (Syzygium luzonense) into sweets, with a focus on inhibiting the growth of Escherichia coli and Staphylococcus aureus. This research employs a quantitative descriptive method, involving the isolation of LAB from candied Pakoba fruit samples and subsequent testing of their antibacterial activity against the aforementioned pathogens. The barrier zone formed around the wells is then measured. The data from the study showed that BAL isolation from Pakoba fruit candied was obtained from as many as 10 isolates, and antibacterial activity tests showed that 10 BAL isolates produced antibacterial compounds with inhibitory diameters of 7.6 – 15.3 mm. The isolates with the greatest antibacterial activity are  LAB isolate MP(1)5.2  for E. coli and LAB isolate MP(3)6.3 isolates for S. aureus. Based on the results of identification using the profile matching method, it was shown that the selected LAB isolates, namely MP(1)5.2 and MP(3)6.3, were classified in the genus Lactobacillus with the characteristics of stem cell shape, single cell arrangement, catalase-negative, non-motile, did not form spots, and did not produce gas from glucose.
Lactic Acid Bacteria That Produce Antibacterial Compunds on Candied Pakoba Fruit Syzygium luzonense Runtuwene, Kharly; Lawalata, Helen; Posumah, Dany; Satiman, Utari; Moko, Emma
Indonesian Biodiversity Journal Vol. 6 No. 2 (2025): August 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/srqbqw88

Abstract

Lactic Acid Bacteria (LAB) are a group of Gram-positive, cocci or rod-shaped microorganisms characterized by their catalase-negative phenotype. These bacteria are known to produce various antibacterial compounds, including organic acids, hydrogen peroxide, carbon dioxide, and bacteriocins, which exhibit bacteriostatic or bactericidal properties against pathogenic bacteria. The aim of this study is to isolate LAB that produce antibacterial compounds during the fermentation of Pakoba fruit (Syzygium luzonense) into sweets, with a focus on inhibiting the growth of Escherichia coli and Staphylococcus aureus. This research employs a quantitative descriptive method, involving the isolation of LAB from candied Pakoba fruit samples and subsequent testing of their antibacterial activity against the aforementioned pathogens. The barrier zone formed around the wells is then measured. The data from the study showed that BAL isolation from Pakoba fruit candied was obtained from as many as 10 isolates, and antibacterial activity tests showed that 10 BAL isolates produced antibacterial compounds with inhibitory diameters of 7.6 – 15.3 mm. The isolates with the greatest antibacterial activity are  LAB isolate MP(1)5.2  for E. coli and LAB isolate MP(3)6.3 isolates for S. aureus. Based on the results of identification using the profile matching method, it was shown that the selected LAB isolates, namely MP(1)5.2 and MP(3)6.3, were classified in the genus Lactobacillus with the characteristics of stem cell shape, single cell arrangement, catalase-negative, non-motile, did not form spots, and did not produce gas from glucose.
Identification of Endophytic Bacteria from Water Hyacinth (Eichhornia crassipes) in Lake Tondano as Potential Agents for Lead Bioremediation Worang, Margi; Lawalata, Helen J.; Tengker, Anita; Roring, Verawati; Posumah, Dany; Rampengan, Mercy
Advances in Tropical Biodiversity and Environmental Sciences Vol. 10 No. 1 (2026): February 2026 (in progress)
Publisher : Institute for Research and Community Services Udayana University (LP2M Universitas Udayana)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2026.v10.i01.p04

Abstract

Water hyacinth (Eichhornia crassipes) is a herbaceous plant known to accumulate heavy metals from polluted waters. In Lake Tondano, this plant grows abundantly and helps reduce lead (Pb) levels through bioremediation. This study aimed to identify the genera of endophytic bacteria from water hyacinth (E. crassipes) that have potential as bioremediation agents for lead. This study employed an exploratory, descriptive, quantitative laboratory approach. Samples were collected from E. crassipes plants found in Lake Tondano at three different locations. The isolation method used was direct plating. The research stages included bacterial isolation, lead resistance testing at 50 ppm, lead reduction testing at 10, 20, and 30 ppm, lead concentration analysis, and bacterial identification using morphological and biochemical tests. Three isolates (EG.A1.5, EG.A2.4, and EG.D3.4) were found to be resistant and capable of reducing lead levels. The reduction percentages reached 35.9%, 72.2%, and 55.5%, respectively. Based on morphological and biochemical characteristics, the bacterial genera of three isolates were identified as Salmonella, Enterobacter, and Bacillus.