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All Journal Biospecies Jurnal Biologi Udayana AL KAUNIYAH Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Jurnal Riau Biologia Jurnal Pengabdian UntukMu NegeRI Jurnal Biologi Tropis Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Journal of Community Engagement Research for Sustainability
Dyah Iriani
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Histochemical Test of root, petiole and leaf of Kelembak (Rheum officinale Baill.) Nurhasanah Nurhasanah; Dyah Iriani
Jurnal Biologi Tropis Vol. 21 No. 3 (2021): September - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i3.2858

Abstract

Kelembak (Rheum officinale Baill.) is perennial shrub with pharmacological activities, and well-known as one of traditional herbal medicine used widely in the world. The phytochemical study, as well as histochemical test, is pivotal to determine the potency of herbs used for disease treatment. Based on its secondary metabolite content.  Histochemical test of R.officinale vegetative organs had not been reported. This study aims to determine the content of compounds in the vegetative organs of kelembak and provide information on the anatomical position of each compound in root, petiole and leaf. The preparation of histochemical test used freehand section method. Each sample was made in three replications for this test. A total of four secondary metabolites were examined in this study, i.e. alkaloid, tannin, lipid and flavonoid. The positive content of each compound was indicated by the coloration change. The specimens were then observed and photographed using light microscope.  The results showed that the root, petiole and leaf showed positive content of alkaloids, tannins, lipids and flavonoids. However, the anatomical position of each secondary metabolites are different among root, petiole and leaf. The results of this study provide the first information of histochemistry vegetative organs of Rheum officinale.
Stomatal Characteristics of 5 Citrus L. Species (Rutaceae) From Pekanbaru, Riau Province Nery Sofiyanti; Putri Intan Wahyuni; Dyah Iriani
Jurnal Biologi Tropis Vol. 22 No. 1 (2022): January - March
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i1.3100

Abstract

Stomata of leaf is one of the important trait in plant taxonomic study. This trait can be used to characterisize the members of a plant group including Citrus.This genus is one of fruit plant that commonly known in Pekanbaru, Riau. The aim of this study was to examine the characteristic of stomata of 5 Citrus species from Pekanbaru, Riau Province, Indonesia. Leaves were collected from the field. The stomatal preparations were conducted using replica method. Stomata were then observed and photographed using Mikroskop Binokuler Olympus CX23 and Mikroskop LCD Digital Celestron Model 44340. The results show that stomata of all Citrus species examined in this study shows the similar type, hypostomatic with reniform-shaped stomata. However, the variations are found in stomata density and index, as well as the length and length of stomata. C. hystrix and C. aurantifolia have the lowest (419.89/1 mm²).), and the highest stomatal density (685.89/1 mm²).), respectively. The size of stomata varies within the species. C. microcarpa has the biggest stomata size (20.5 x 18.5 µm).  Stomatal density of Citrus examined in this study are high (> 500 / mm2) in C. aurantifolia and C. limon) and medium (300 - 500 / mm2) in C. amblicarpa, C. hystrix and C. microcarpa. The density, index and size of stomata can be used to characterize each Citrus members that examined in this study.
Kajian Anatomi-Histokimia Tangkai Daun dan Karakteristik Epidermis Pucuk Merah (Syzygium myrtifolium Walpp. – Myrtaceae) Nery Sofiyanti; Dyah Iriani; Asri Ria Lestari
Buletin Anatomi dan Fisiologi Volume 7, Nomor 2, Tahun 2022
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.7.2.2022.83-90

Abstract

Tanaman pucuk merah (Syzygium myrtifolium Walp.) merupakan salah satu tanaman  yang termasuk dalam famili Myrtaceae. Penelitian ini bertujuan melakukan kajian  anatomi-histokimia petiole dan karakteristik epidermis daun pucuk merah. Sampel yang diambil adalah daun pucuk merah yang sudah dewasa. Penelitian ini dilakukan pada bulan April sampai Juni 2022. Pembuatan preparat anatomi dilakukan dengan metode parafin, sedangkan uji histokimia dilakukan pada senyawa alkaloid, tanin, flavonoid, dan lipid dengan metode kualitatif. Pengamatan karakteristik epidermis dilakukan dengan metode paradermal. Hasil pengamatan anatomi penampang melintang petiole pucuk merah menunjukan bentuk  agak oval, membulat pada bagian dorsal dan bersayap pada bagian ventral. Kutikula terdapat di sebelah luar epidermis. Korteks terdiri dari lapisan hipodermis dan parenkim. Hipodermis tersusun dari 4 – 5 lapisan  sel-sel kolenkim memanjang, dan terdapat rongga sekresi berisi minyak. Parenkim tersusun dari 17 – 19 sel sel poligonal.  Berkas pembuluh berada di tengah dengan tipe bikolateral. Hasil uji histokimia petiole  menunjukkan hasil positif flavonoid, alkaloid, tanin, dan lipid. Stomata pucuk merah termasuk hipostomatik dengan tipe parasitik. Sel-sel epidermis poligonal dengan dinding sel bergelombang (undulate). Hasil kajian ini memberikan informasi tambahan mengenai anatomi, histokimia dari petiolus pucuk merah serta memberikan informasi karakteristik epidermis dan stomata tanaman ini. The red tip plant (Syzygium myrtifolium Walp.) belongs to Myrtaceae family. The aim of this study was to investigate the anatomical-histochemical of ptiole as well as epidermal chrakteristic of red tip plant. Leaf samples were collected sixth node from the top. This study had been conducted from April to June 2022. The anatomical preparation used paraffine method. Histochemistry test was carried out for alkaloid, tannin, flavonoid and lipid using qualitative method. Epidermal charateristic was observed using paradermal method. The anatomical result shows that the cross section of petiole of red tip plant is slightly ovale-shaped, with rounded part at dorsal side dan winged-part at ventral side. The outermost part is cuticle layer. Cortex is consisted of hipoderm and parenchyma layers. Hipoderm is arranged by 4 to 5 layers of elongated cholencym cells, with secretion structure or oil gland. Parenchym is consisted of 17 – 19 layers of polygonal cells. Vascular bundle is located in the center, with bicholateral type. The histochemistry test indicated positive content of flavonoid, alkaloid, tannin and lipid. The stomata of red tip plant is hypostomatic with paracytic type. The epiderm cells are polygonal with undulate cell walls.The result of this study provide the additional information of anatomy, histochemistry of petiole of red tip land, and also provide the information of epidermal characteristic of this plant.
The Effect of Yeast, NPK and Fermentation Time in Bioethanol Fermentation from “Abacaxi” Pinapple Pericarp Nery Sofiyanti; Dyah Iriani; Putri Intan Wahyuni; Nurul Idani; Puji Lestari
Jurnal Biologi Tropis Vol. 22 No. 4 (2022): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i4.4312

Abstract

Bioethanol is promising alternative fuel due to its less effect to environment. It is produced from bio-sources, such plant materials. Yeast and NPK have been reported as the ingredients in affecting bioethanol fermentation. The aim of this study was to examined the effect of yeast and NPK in bioethanol fermentation using the pericarp of “Abacaxi” pinnapple cultivar (Ananas comosus cv. “Abacaxi”). The pericarp juice of “Abacaxi” pineapple was made by blending the fresh pericarp and aquadest (1 : 1.5). The juice was then mix with dry commercial yeast (1 g, 2.5 g and 5 gr) and NPK  (1 g, 2 g, 3 g). A total of 9 treatments were used in fermentation.  Each treatment was replicated three times, brought the total sample was 27. The percentage of bioethanol for each treatment was measure using alcoholmeter for six days (24 h, 48 h, 72 h, 96 h, 120 h and 144 h). The result show that the percentage of bioethanol produced in this study varies among different treatment and fermentation time. Yeast and NPK gave significant effect in bioethanol fermentation, with the combination of 5 g yeast and 3 g NPK gave the highest percentage of bioethanol in 144 h of fermentation. Based on the result of this study, the pericarp of “Abacaxi” pineapple is potential bio-source for bioethanol fermentation.
Kajian anatomi, histokimia, dan karakteristik epidermal daun sawo kecik (Manilkara kauki (L.) Dubard - Sapotaceae) Nery Sofiyanti; Dyah Iriani
Jurnal Biologi Udayana Vol 27 No 1 (2023): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2023.v27.i01.p02

Abstract

Manilkara kauki (L.) Dubard (sawo kecik) merupakan salah satu jenis pohon penghasil buah dari genus Manilkara Adans. (Sapotaceae). Jenis ini termasuk jarang dijumpai di Sumatera, termasuk di Provinsi Riau. Tujuan dari kajian ini adalah untuk mengetahui struktur anatomi dan histokimia daun M. kauki serta karakteristik epidermalnya. Pembuatan preparat anatomi dan histokimia dilakukan dengan menggunakan preparat segar dari 3 bagian daun M. kauki yaitu tangkai daun, ibu tulang daun dan helai daun. Senyawa metabolit sekunder yang diuji pada kajian histokimia adalah alkaloid, flavonoid, tanin dan lipid. Pembuatan preparat epidermal dilakuan dengan metode replika. Pengamatan struktur anatomi, histokimia dan karakteristik epidermal daun diamati dan didokumentasikan menggunakan mikroskop digital Olympus. Hasil kajian anatomi tangkai daun dan ibu tulang daun menunjukan karakteristik pada setiap jaringan, yaitu kutikula, epidermis, berkas pengangkut utama yang tersusun dari sklereid, floem dan xilem, serta adanya artificial laticifier pada bagian tengah. Berkas pengangkut tambahan hanya dijumpai pada tangkai daun. Struktur anatomi helai daun menunjukan adanya sklereid dan sel tanin pada mesofil. Hasil kajian histokimia menunjukan hasil posisif pada setiap organ yang diuji, namun konsentrasi setiap senyawa pada jaringan yang diamati berbeda-beda. Tipe stomata pada M. kauki adalah hipostomatik. Hasil kajian ini memberikan infomasi tambahan pada struktur anatomi, histokimia dan epidermal dari genus Manilkara.
Studi Poliembrio Biji Jeruk Sambal (Citrus x amblycarpa (Haask.) Osche) Sebagai Batang Bawah Sari, Herlinda; Iriani, Dyah
Buletin Anatomi dan Fisiologi Volume 9, Nomor 2, Tahun 2024
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.9.2.2024.111-120

Abstract

Citrus x amblycarpa sering dimanfaatkan oleh masyarakat di Kecamatan Lubuk Alung, Kabupaten Padang Pariaman, Sumatera Barat, sebagai batang bawah karena toleran terhadap Citrus Tristeza Virus (CTV). Penelitian ini bertujuan untuk mempelajari tingkat poliembrioni biji jeruk sambal dan mengamati viabilitas biji dan vigor bibit jeruk sambal. Penelitian dilakukan dengan mengamati jumlah embrio menggunakan mikroskop dan dengan penyemaian biji. Ekstraksi biji dan pengamatan dilakukan di Laboratorium Botani Jurusan Biologi FMIPA Universitas Riau. Penyemaian biji dilakukan di Jalan Kubang Jaya, Pekanbaru. Hasil penelitian ini menunjukkan persentase poliembrioni biji jeruk sambal pada pengamatan mikroskopis sebesar 27,85% dan pada biji monoembrioni sebesar 72,14%. Hasil semaian menunjukkan persentase multiple seedling sebesar 14%, semaian tunggal 75,33%, dan bibit mati 10,66%. Rerata laju perkecambahan biji jeruk sambal paling cepat (9,3 hari) dan paling lambat (12,2 hari). Indeks kecepatan perkecambahan paling cepat pada kategori bobot buah kecil yaitu 6,4 dengan laju perkecambahan 12,2 hari. Rerata tinggi bibit, jumlah daun, panjang akar tertinggi dihasilkan oleh bobot buah besar dengan rerata tinggi bibit sebesar 7,21cm±2,33; jumlah daun sebesar 2,64 helai± 0,71; panjang akar tunggang sebesar 4,38 cm±1,9; dan jumlah akar cabang sebesar 2,8±1,31. Biji jeruk sambal bersifat poliembrioni dan  persentase perkecambahan sebesar 89,33%, rerata persentase poliembrioni sebesar 15,66%. Jeruk sambal always used by the community in Lubuk Alung District, Padang Pariaman Regency, West Sumatra, as a rootstock because it’s tolerant to Citrus Tristeza Virus (CTV). This research aims to study the level of polyembryony in chili orange seeds and observe seed viability and vigor of chili orange seedlings.  This research was carried out by observing the number of embryos using a microscope and observing the number of embryos by sowing seeds. Seed extraction and observations were carried out at the Botany Laboratory, Biologi Department, FMIPA, Riau University. Seed sowing is carried out on Kubang Jaya, Pekanbaru.The results of this story showed that the percentage of polyembryonic chili orange seeds on microscopic observations was 27.85% and that of monoembryonic seeds were 72.14%.  The Seedling results showed that the percentage of multiple seedlings was 14%, single seedlings were 75.33%, and dead seedlings were 10.66%.  The germination rate was the fastest (9.3 days) and the slowest (12.2 days). The fastest germination speed index in the small fruit weight category is 6.4 with a germination rate of 12.2 day The highest average seed height, number of leaves, root length was produced by large fruit weight with an average seed height is 7.21cm ± 2.33; number of leaves of 2.64 ± 0.71; tap root length of 4.38cm ± 1.9; and the number of branch roots was 2.8 ± 1.31. Chili oranges seeds are polyembryonic and the germination percentage is 89.33%, the average polyembryonic percentage is 15.66%.
Analisis Struktur Anatomi dan Histokimia Daun Buah Makasar (Brucea javanica (L.) Merr.) Azlina, Rosi; Iriani, Dyah
Buletin Anatomi dan Fisiologi Volume 9, Nomor 2, Tahun 2024
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.9.2.2024.137-147

Abstract

Buah Makasar merupakan spesies dari famili Simaroubaceae yang memiliki habitus perdu dan berdaun majemuk. Daun Buah Makasar memiliki potensi sebagai tanaman obat. Oleh karena itu penting dilakukan kajian struktur anatomi untuk mengetahui struktur pada organ daun Buah Makasar dan uji histokimia untuk mengetahui keberadaan metabolit sekunder pada daun Buah Makasar yang berpotensi sebagai obat.  Penelitian ini bertujuan untuk mengkarakterisasi anatomi dan histokimia daun Buah Makasar dan menganalisis potensinya sebagai tanaman obat. Metode yang digunakan adalah metode parafin, metode paradermal, dan histokimia. Struktur anatomi diamati mulai dari ibu tulang daun, intercostal, tepi daun melalui sinus dan angulus, dan tangkai daun. Hasil kajian anatomi menunjukkan karakteristik pada setiap jaringan, yaitu epidermis, jaringan dasar, dan berkas pengangkut tipe kolateral. Tepi anak daun tersusun oleh Jaringan mesofil yang terdiferensiasi menjadi jaringan palisade dan jaringan bunga karang. Ibu tulang daun dan tangkai daun memiliki berkas pengangkut utama juga berkas pengangkut tambahan (accessory vascular bundle) berukuran kecil berada pada bagian adaksial. Stomata hipostomatik dengan tipe parasitik dengan kerapatan stomata 26,31±2,64/mm2. Trikoma dijumpai pada bagian adaksial dengan kerapatan trikoma non glandular lebih tinggi 30,97±16,22/mm2 dibandingkan trikoma glandular 1,27±1,27/mm2. Analisis histokimia menunjukkan hasil positif terhadap senyawa alkaloid, flavonoid, tanin, lipofilik, dan terpenoid pada setiap organ yang diuji, namun kepekatan warna setiap senyawa pada jaringan yang diamati berbeda-beda. Buah Makasar is a species of the Simaroubaceae family that has a shrub habitus and compound leaves. Buah Makasar’s leaf have potential as medicinal plants. Therefore, it is important to study the anatomical structure to determine the structure of the leaf organs of Buah Makasar and histochemical test to determine the presence of secondary metabolites in the leaves of Buah Makasar that have potential as drugs. The methods used were paraffin, paradermal, and histochemical method. Anatomical structures were observed on midrib, intercostal, margin through sinus and angulus, and petiole. The results of the anatomical study showed characteristics in each tissue, namely the epidermis, ground tissue, and collateral-type transport bundles. Anatomical structure of leaf margin is composed of mesophyll tissue which is differentiated into palisade tissue and spongy tissue. Midrib and petiole have main transport bundles as well as small accessory vascular bundles in the adaxial part. Stomata are hypostomatic with parasitic type with stomatal density of 26.31±2.64/mm2. Trichomes were found in the adaxial part with a higher density of non-glandular trichomes 30.97±16.22/mm2 compared to glandular trichomes 1.27±1.27/mm2. Histochemical analysis showed positive results for alkaloids, flavonoids, tannins, lipophytes, and other compounds.
Sosialisasi Tanaman Penurun Gula Darah Di Desa Koto Ringin Kecamatan Mempura Kabupaten Siak Radith Mahatma; Dyah Iriani; Yusfiati, Yusfiati; Nery Sofiyanti; Roza Elvyra; Fadel Nugraha
Jurnal Pengabdian UntukMu NegeRI Vol. 8 No. 3 (2024): Pengabdian Untuk Mu negeRI
Publisher : LPPM UMRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jpumri.v8i3.8055

Abstract

Negative impact of the development over time in the health aspect is the change in lifestyle or unhealthy dietary habits, particularly through the consuming of instant foods, which can trigger diabetes. Excessive sugar consumption can lead to increasing weight and blood sugar levels, and potentially cause type-2 diabetes (DM). The community of Koto Ringin Village has developed a family medicinal plant program (TOGA) as part of a strategy to improve community health. This program demonstrates proactive steps in addressing health challenges in Koto Ringin Village, both through disease management and community-based health initiatives like TOGA. The purpose of this community service is to improve the understanding of the PKK (Family Welfare Movement) members of Koto Ringin Village by educating them about plants that are useful for lowering blood sugar levels. This community service is also intended for transferring knowledge from the academic environment to the community at large, especially to the members of PKK Koto Ringin Village, Mempura District, Siak Regency, Riau Province. This activity was attended by 18 women from the PKK of Koto Ringin. The results of the activity showed that the participants’ knowledge improved from 41.1 to 90.0.
Pelatihan Pembuatan Hand Sanitizer Alami Di Kelurahan Sumahilang Kecamatan Pekanbaru Kota, Pekanbaru Iriani, Dyah; Sofiyanti, Nery; Elvyra, Roza; Chahyadi, Ennie; Arini, Arini
Jurnal Pengabdian UntukMu NegeRI Vol. 5 No. 2 (2021): Pengabdian Untuk Mu negeRI
Publisher : LPPM UMRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jpumri.v5i2.3106

Abstract

Pandemi Covid-19 yang disebabkan oleh virus Corona telah menjadi permasalahan global, tidak terkecuali di Pekanbaru. Hampir seluruh lokasi di Pekanbaru telah terdampak akibat adanya pandemi Covid-19, salah satunya di Kelurahan Sumahilang, Kecamatan Pekanbaru Kota, Kota Pekanbaru. Untuk mengatasi masalah Covid-19, pemerintah menghimbau masyarakat untuk rajin mencuci tangan dengan sabun. Namun terkadang jika kita bepergian, tidak di semua tempat dan ruangan terdapat air dan sabun, maka hand sanitizer atau cairan antiseptik bisa menjadi alternatif untuk mencuci tangan yang bisa diandalkan. Alkohol sebagai komponen utama hand sanitizer, berfungsi sebagai antiseptik pada permukaan kulit. Alkohol pada pemakaian berulang menyebabkan kekeringan dan iritasi pada kulit. Agar penggunaan alkohol efektif tanpa menimbulkan dampak bagi penggunanya, maka perlu dilakukan inovasi produk antiseptik handsanitizer dengan menggunakan ekstrak tanaman yang mengandung sifat antibakteri. Ekstrak daun sirih dan jeruk nipis dapat memberikan solusi akibat yang ditimbulkan dari hand sanitizer yang menggunakan alkohol. Kegiatan Pelatihan Pembuatan Handsanitizer Alami di Kelurahan Sumahilang dilakukan pada hari Rabu 18 Agustus 2021. Kegiatan ini diikuti 20 peserta yang berasal dari ibu-ibu Posyandu dan PKK Kelurahan Sumahilang. Hasil kegiatan menunjukkan pengetahuan peserta pelatihan mengenai pembuatan hand sanitizer alami meningkat dari 20% menjadi 85% Kata kunci: Covid-19, hand sanitizer alami, jeruk nipis, kelurahan Sumahilang, sirih
Penerapan Tehnik Pengolahan Limbah Rumah Tangga Dengan Cacing Tanah Pada Kelompok Tani Kampung Bunga Raya Siak Yusfiati, Yusfiati; Chahyadi, Eni; Iriani, Dyah
Journal of Community Engagement Research for Sustainability Vol. 2 No. 2 (2022): Maret
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.907 KB) | DOI: 10.31258/cers.2.2.78-83

Abstract

The application how processing to fertilizer from household waste with earthworms aims to add positive and productive activities among the farmers of Kampung Bunga Raya in order to increase the economic value of farmers' income.The method used was counseling and direct practice in the field with 19 participants from farmer groups. The results obtained are 1 kg of household waste can produce 2 kg of organic fertilizer and 500 grams of worms. The fertilizer obtained can be used for oil palm plantations and gardening of farming communities in Bunga Raya village, Bunga Raya district, Siak. The Cultivation of earthworms are using household waste can overcome the problem of availability of fertilizer for plantations.
Co-Authors Arini Arini Asma Sari Asri Ria Lestari Azlina, Rosi Chahyadi, Eni Desi Mulyanti Dwijowati Asih ENNIE CHAHYADI, ENNIE Fadel Nugraha Fitmawati ' Guspa Yuriza Indah Permatasari Iwan Simatupang Laksono Trisnantoro MARPAUNG, AFNI ATIKA Mayta Novaliza Isda Nery Sofiyanti Norsamsi NORSAMSI Nurul Idani Puji Lestari Purwoko, Agus Putri Intan Wahyuni Radith Mahatma Roza Elvyra Sari, Herlinda Sujarwati ' Tiara Maulidza Riyani Tirtha Juliana Widianti ' Yusfiati, Yusfiati