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All Journal Journal of Food and Pharmaceutical Science Journal of the Medical Sciences (Berkala Ilmu Kedokteran) INDONESIAN JOURNAL OF PHARMACY Journal of Community Empowerment for Health Journal of Food and Pharmaceutical Sciences SWARNA: Jurnal Pengabdian Kepada Masyarakat
Prasetyastuti
Departemen Biokimia, Fakultas Kedokteran, Kesehatan Masyarakat, Dan Keperawatan Universitas Gadjah Mada, Yogyakarta, Indonesia
Religion Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health

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Journal : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

 1 
Effects of quercetin on the nicotine-induced oxidative status in male Wistar rats: study on c-reactive protein (CRP) and malondialdehyde (MDA) concentrations Deri Riskiyanti Tallo Manafe; Denny Agustiningsih; . Prasetyastuti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 2 (2016)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.115 KB) | DOI: 10.19106/JMedSci004802201602

Abstract

ABSTRACTNicotine can cause atherosclerosis by activating nuclear factor-kB (NF-kB) pathway lead to induce proinflammatory cytokines release as C-reactive protein (CRP) main regulators. The increase of CRP can induce reactive oxygen species (ROS) and increase of malondialdehyde (MDA). Quercetin has been proven to have antiinflammatory and antioxidant effects. This study was conducted to evaluate effect of quercetin on serum CRP and MDA concentrations in rats induced by nicotine. This was a true experimental study with post test only control group design. Thirty six of male Wistar rats were divided into six groups. Group I as normal control received 1 mL/kg BW of NaCl 0.9% solution. Group II as negative control received 2 mg/kg BW of nicotine and Group III as positive control received 2 mg/kg BW of nicotine and atorvastatin at dose of 5 mg/kg BW. Group IV-VI as treatment groups received 2 mg/kg BW of nicotine and quercetin at dose of 25; 50 or 100 mg/kg BW, respectively. Nicotine was given subcutaneously whereas atorvastatin and quercetin were given orally once per day for 28 days, consecutively. Serum CRP and MDA concentrations were measured using Rat hs-CRP ELISA kit and TBARS assay kit, respectively. Data were analyzed using analysis of variance (ANOVA) continued using LSD post-hoc test. The results showed that quercetin reduced serum CRP and MDA concentrations in dose dependent manner. Serum CRP concentration on Group V (173.39 ± 34.85 ng/mL) and Group VI (114.15 ± 43.62 ng/mL) were significantly lower than that Group II (244.77 ± 37.95 ng/mL) (p<0.05). Furthermore, serum MDA concentration on Group IV (5.95 ± 0.11 mmol/mL), Group V (3.93 ± 0.09 mmol/mL) and Group VI (2.14 ± 0.09 mmol/mL) were significantly lower than that Group II (7.29 ± 0.06 mmol/mL) (p<0.05). In conclusion, quercetin reduces the nicotine-induced oxidative status in rats. 
Effects of 7-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chroman-4-one on serum levels of antioxidant enzymes in hyperlipidemic rats . Prasetyastuti; Noviyanty Indjar Gama
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 55, No 2 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19106/JMedSci005502202301

Abstract

Hyperlipidemia triggers oxidative stress caused by an imbalance between oxidant and antioxidant levels due to the excess production of reactive oxygen species (ROS). The increase of ROS can decrease antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). 7-OH-2-(4-OH-3-Methoxyphenyl)-chroman-4-one is exogenous antioxidants isolated from mahogany seeds (Swietenia macrophylla King). This study aimed to evaluate the effects of the 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one on serum levels of SOD, CAT, and GPx in hyperglycemic rats. Thirty-six male Wistar rats (Rattus norvegicus) were divided into the following six groups: (N) normal group, (HL) hyperlipidemia group, (P) hyperlipidemia group with simvastatin, F10, F30, and F90 hyperlipidemia group with 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one 10, 30 and 90 mg/200g body weight (BW), respectively.  Hyperlipidemia was induced by feed enriched with cholesterol and cholic acid. Treatments were administered orally by gavages. After 4 weeks of treatments, blood sample was drawn and serum levels of SOD, CAT, and GPx enzymes were analyzed using a spectrophotometric method. Serum levels of SOD, CAT, and GPx in hyperlipidemic rats treated with 7-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chroman-4-one at dose 10, 30 and 90 mg/200g BW were higher than HL group. In addition, no significantly different on serum SOD and CAT between group F90 and group P was observed (p>0.05)
Co-Authors . Suhartini Abrory Agus Cahya Pramana Ahmad Hamim Sadewa Ainun Nisa Annisa Nurul Huda Arta Farmawati Arta Farmawati Ayunda, Rahmah Dara Deri Riskiyanti Tallo Manafe Dianandha Septiana Rubi Dina Septari Anindyah Hendro Widagdo Ida Bagus Gd. Surya Putra Pidada Indra Sari Kusuma Harahap Kusumo Dananjoyo Lily Arsanti Lestari Martiana Suciningtyas Ngadikun - Ngadikun Ngadikun Noviyanty Indjar Gama Nurliyani Pramudji Hastuti Pramudji Hastuti Pramudji Hastuti Pramudji Hastuti Qisthira Swasti Amirina Sri Sutarni Sunarti Sunarti Sunarti Sunarti Tasmini Tasmini Tasmini Tasmini Widiyani Indrianingrum