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Amelia Hana
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Journal : Jurnal Veteriner

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Fourier Transform Infrared Sebagai Metode Alternatif Penetapan Tingkat Stres pada Sapi (FOURIER TRANSFORM INFRARED AS AN ALTERNATIVE TOOL FOR DETERMINING OF STRESS IN COW) Pudji Astuti; Claude Mona Airin; Slamet Widiyanto; Amelia Hana; Hera Maheshwari; Luthfiralda Sjahfirdi
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Stress in animal is a condition of nonspesific discomfort which cause of non-specific immune defects,failure of reproduction, and decreased of meat carcass until the death of  animals. To determine stress ofcattle,  it will be invented stress detector using a non-invasive method based on the spectroscopy FourierTransform Infrared (FTIR). Basically,  FTIR will detect component in compound of cathecolamine andcortisol as ketone (= O) and methyl (= CH 3). Furthermore, each group of components will be detected indifferent of absorbant and wavelength.   The results showed that average level of cortisol in female beefcattle durimg resting eriod was 38,48±21,53 ng/dL, on time of slaughtering were 116,88±112,59 ng/dL. Forbull, which were resting  20,42±9,25 ng/dL; when animal was slaughtered level of cortisol was  67,61±41,62ng/dL. Using FTIR, it was showed that compound of metil was absorbed well. Animals with udder stresscondition have been recorded on the wave lenght of 2777-3456 nm.   It has been concluded level of cortisolon cattle which were resting is significantly different from animal which were slaughtering P(< 0.05),where cortisol would increase drastically. Using Calibration of FTIR indicated resting animals only havefewer value of absorbance than animals which slaughtered.  FTIR is a very prospect method for makingstress indicator.
Respons Peristalsis dan Neuron Mienterik Nitrergik Usus Halus Kelinci yang Diinfeksi Eimeria magna Amelia Hana; Soesanto Mangkoewidjojo; Siti Isrina Oktavia Salasia2,; Dwi Liliek Kusindarta3
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Penelitian ini bertujuan untuk mengetahui respon usus kelinci yang diinfeksi Eimeria magna denganmengamati frekuensi dan amplitudo kontraksi peristalsis, dan jumlah neuron mienterik nitrergik usushalus. Hewan percobaan yang digunakan adalah 60 ekor kelinci jantan lokal sehat umur 5 bulan dengankisaran berat badan 1,5-1,8 kg, dan bebas koksidiosis. Seluruh kelinci percobaan diadaptasikan dengankondisi lingkungan penelitian selama 7 hari dengan pakan pellet dan air minum ad libitum. Kelincidipelihara dalam kandang individual. Enam puluh ekor kelinci tersebut dibagi secara acak menjadi 3kelompok masing-masing 20 ekor. Kelompok I sebagai kontrol (K-0) diberi 1,0 ml akuades/ekor per oral,kelompok II (K-10) diinfeksi 10x106 ookista E. magna/ekor per oral dosis tunggal, dan kelompok III (K-20)diinfeksi 20x106 ookista E. magna /ekor per oral dosis tunggal. Pascainfeksi setiap hari 4 ekor per kelompokdianestesi dengan uretan (1,55 g/kg BB dalam larutan 25%, secara intraperitoneal), kemudian dibedah,diambil segmen usus halus (duodenum, jejunum, dan ileum). Segera setelah itu kelinci dibunuh dengancara dislokasi cervikal. Segmen usus halus diukur gerak peristalsis secara elektromiografik. Selanjutnyasampel tersebut dibuat preparat histokimia dengan teknik pewarnaan Nicotinamide Adenine DincleotidePhosphate-diaphorase (NADPH-d) untuk mengetahui jumlah neuron mienterik nitrergiknya. Data frekuensidan amplitudo kontraksi peristalsis, dan jumlah neuron mienterik nitrergik usus halus kelinci dianalisissecara statistika dengan sidik ragam dan uji-t (LSD). Hasil penelitian menunjukkan bahwa infeksi10x106 dan 20x106 ookista E. magna dapat menyebabkan timbulnya peningkatan frekuensi peristalsisusus halus (p<0,01), penurunan amplitudo kontraksi usus halus (P<0,01), dan penurunan jumlah neuronmienterik nitrergik usus halus (P<0,01) dibandingkan kelompok kontrol (K-0). Dari hasil disimpulkanbahwa infeksi ookista E. magna dapat menyebabkan peningkatan frekuensi peristalsis, penurunanamplitudo kontraksi, dan penurunan jumlah neuron mienterik nitrergik usus halus kelinci.
Co-Authors . Sumartono Calude Mona Airin Claude Mona Airin Dwi Liliek Kusindarta3 HERA MAHESHWARI LUTHFIRALDA SJAHFIRDI Nuraini Rahmawati Pradityo Yoga Wibowo Pudji Astuti PUDJI ASTUTI Siti Isrina Oktavia Salasia2, Slamet Widiyanto Soesanto Mangkoewidjojo Tri Wahyu Pangestiningsih Yuda Heru Fibrianto