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All Journal JBIO: jurnal biosains (the journal of biosciences) Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Saintia Biologi JURNAL PENELITIAN SAINTIKA JURNAL PENGABDIAN KEPADA MASYARAKAT Jurnal Penelitian Pendidikan IPA (JPPIPA) Abdimas Talenta : Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Chemistry Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) KLOROFIL: Jurnal Ilmu Biologi dan Terapan Jurnal Pembelajaran dan Biologi Nukleus KLOROFIL: Jurnal Ilmu Biologi dan Terapan Bioscientist : Jurnal Ilmiah Biologi
Isnaini Nurwahyuni
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Sumatera Utara
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Social Sciences Other

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ISOLASI DAN UJI POTENSI ISOLAT BAKTERI PELARUT FOSFAT DAN BAKTERI PENGHASIL HORMON IAA (Indole Acetic Acid) TERHADAP PERTUMBUHAN KEDELAI (Glycine max L.) PADA TANAH KUNING Dessy Merry Silitonga; Nunuk Priyani; Isnaini Nurwahyuni
Saintia Biologi Vol 1, No 2 (2013)
Publisher : Saintia Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.567 KB)

Abstract

Biofertilizer is a safe alternative fertilizer instead of various chemical fertilizers for increasing plant productivity which can minimize the ecological damage. There are plant symbiotic-soil bacteria which can solubilize phosphate or produce IAA. The objectives of this research are to determine the best soil bacteria in producing IAA or solubilizing phosphate and to know their roles in promoting plant growth in unfertile soil. As many as 8 isolates of phosphate solubilizing bacteria and 5 isolates of IAA producing bacteria were found. Among those isolates, there were 2 potential isolates (P2 & P4) that were able to solubilize phosphate and 1 potential isolate (I3) that was able to produce IAA. Isolate P4 produced holozone as wide as 1.45 cm and isolate P2 was 1.3 cm. Meanwhile, isolate I3 produced the highest concentration of IAA compared to the other isolates, that was 33.3 ppm. These isolates were applicated further on unfertile soil (yellow soil) as a medium for soybean growth. Soybeans have been grown for 10 weeks to observe the effect of those potential isolates on plant growth and productivity. The result showed that application of  isolate I3 was able to promote plant growth and increase plant productivity better than the other isolates as well as controls (fertile and unfertile soil). It promoted the soybean growth such as plant height was 58,8 cm; plant fresh weight was 3.5 g; plant dry weight was 1.55; pod fresh weight was 1.23; pod dry weight was 1.03; the amount of the pod was 3 and the number of the seed was 7.   Keywords : Phosphate solubilizing bacteria, IAA producing bacteria, biofertilizer, soybean.
ANALISIS KARIOTIPE DAN PERTUMBUHAN KACANG KEDELAI (Glycine max L.) SETELAH DIINDUKSI SINAR ULTRAVIOLET Rani Ernita Limbong; Elimasni D; Isnaini Nurwahyuni
Saintia Biologi Vol 1, No 3 (2013)
Publisher : Saintia Biologi

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Abstract

“Karyotype and Growth Analysis of Soybean (Glycine max L.) after Exposed by Ultraviolet light” has been done at Genetic and Molecular Biology Laboratory at Natural Scienes Faculty and the greenhouse at Agriculture Faculty Sumatera Utara University Medan. The objective was to investigate design karyotype and vegetative growth of soybean that induced by UV light. The experimental design was used complete factorial randomized with 2 factors. The first are Ultraviolet (UV) light 3 levels : 10, 20 and 30 Watt and the second is the time of UV exposure with 3 levels : 5, 10, and 15 minutes. Each treatment replicates 2 times and plant that not exposed by UV used as control. The karyotype was observed by squash method. The results showed that the intensity and duration of UV light have no effect on the number of chromosomes but the light has effect on the number of chromosome type. Statistical analysis showed that the increase in UV energy up to 30 Watt and time exposure of 15 minutes, caused the increase in height, number of leaves, number of flowers, the fresh and dry weight of the plant, and chlorophyll concentration compared to untreated plants. Keywords: karyotype, mutation, soybean, ultraviolet.
Perubahan Histologi dan Fisiologi Latisifer pada Tanaman Karet (Hevea brasiliensis Muell Arg.) yang diberi Asam Jasmonat dan Asam Naftalen Asetat Eksogen Radite Tistama; Vahnoni Lubis; Isnaini Nurwahyuni
Buletin Anatomi dan Fisiologi Volume 2, Nomor 1, Tahun 2017
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.2.1.2017.1-10

Abstract

Produktivitas tanaman karet tergantung pada faktor dominan seperti jumlah latisifer dan aktivitas metabolism lateks. Perkembangan histologi dan fisiologi latisifer tanaman karet dipengaruhi oleh jenis klon dan interaksi antara zat pengatur tumbuh eksogen dengan klon. Perubahan histologi dan fisiologi latisifer Hevea brasiliensis diteliti dengan aplikasi asam jasmonat, asam naftalen asetat dan kombinasi keduanya pada permukaan tunas lateral muda. Hasil penelitian ini menunjukkan bahwa zat pengatur tumbuh menginduksi jumlah pembuluh kateks tetapi mengurangi perkembangan tebal kulit dan ukuran sel latisifer. Kombinasi JA dengan NAA paling efektif menginduksi jumlah pembuluh lateks pada klon metabolisme tinggi PB 260. Zat pengatur tumbuh memacu perubahan aktivitas fisiologi pada klon metabolisme rendah IRR 42. Analisis histologi kulit dan fisiologi lateks pada tunas muda tanaman karet dapat digunakan sebagai pendeteksi potensi produktivitas dan sifat metabolisme tanaman karet. Kata kunci: Latisifer Hevea; asam jasmona; asam naftalen asetat; metabolisme lateks
The Development of Reproducible and Selective Uric Acid Biosensor by Using Electrodeposited Polytyramine as Matrix Polymer Manihar Situmorang; Isnaini Nurwahyuni
Indonesian Journal of Chemistry Vol 17, No 3 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (339.143 KB) | DOI: 10.22146/ijc.25818

Abstract

A versatile method for the construction of reproducible and high selective uric acid biosensor is explained. Electrodeposited polytyramine is used as biosensor matrixes due to its compatibility to immobilize enzyme uric oxidase in the membrane electrode. The precise control over the charge passed during deposition of polytyramine allows concomitant control over the thickness of the deposited enzyme layers onto the surface of the electrode. The uric acid biosensor showed a sensitive response to uric acid with a linear calibration curve lies in the concentration range of 0.1–2.5 mM, slope 0.066 µA mM-1, and the limit detection was 0.01 mM uric acid (S/N = 3). The biosensor shown excellent reproducibility, the variation between response curves for uric acid lies between RSD 1% at low concentrations and up to RSD 6% at saturation concentration. Uric acid biosensor is free from normal interference. The biosensor showed good stability and to be applicable to determine uric acid in real samples. Analysis of uric acid in the reference standard serum samples by the biosensor method are all agreed with the real value from supplier. Standard samples were also analyzed independently by two methods: the present biosensor method and the standard UV-Vis spectrophotometry method, gave a correlation coefficient of 0.994. This result confirms that the biosensor method meets the rigid demands expected for uric acid in real samples.
RESPONS PERTUMBUHAN TANAMAN KEDELAI HITAM (Glycine soja L) MELALUI POC AIR TAHU DAN AIR KELAPA Rahma Dina; Isnaini Nurwahyuni; Elimasni Elimasni
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 6, No 2 (2022): KLOROFIL : JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v6i2.12439

Abstract

The Study aims to see used of POC from tofu and coconut water waste has a significant effect in the growth of black soybean (Glycin soja L.) on the parameters of stem height, number of branches and leaves, and the number of stem diameters of black soybean plants. The research was conducted from September to December 2021 in kayu besar street, Batangkuis district. The study used RAK on 2 factorials, namely the treatment of giving POC from tofu and coconut water and concentrations at the levels of 0%, 15%, 20%, and 25%.The data analysis technique used the one way ANOVA test at a significant level of 5%and the DMRT test. The results showed that the use of POC from coconut air and air had a significantly different effect at a concentration of 25% better than other concentrations, namely 20%, 15%, and 0%. This study gave a better effect on the use of POC than those without fertilizers due to the addition of nutrients provided to increase the growth of black soybean plants.Key words: Black Soybean, POC, Water Tofu, Water Coconut. 
RESPONS PERTUMBUHAN TANAMAN KEDELAI HITAM (Glycine soja L) MELALUI POC AIR TAHU DAN AIR KELAPA Rahma Dina; Isnaini Nurwahyuni; Elimasni Elimasni
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 6, No 2 (2022): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v6i2.12439

Abstract

The Study aims to see used of POC from tofu and coconut water waste has a significant effect in the growth of black soybean (Glycin soja L.) on the parameters of stem height, number of branches and leaves, and the number of stem diameters of black soybean plants. The research was conducted from September to December 2021 in kayu besar street, Batangkuis district. The study used RAK on 2 factorials, namely the treatment of giving POC from tofu and coconut water and concentrations at the levels of 0%, 15%, 20%, and 25%.The data analysis technique used the one way ANOVA test at a significant level of 5%and the DMRT test. The results showed that the use of POC from coconut air and air had a significantly different effect at a concentration of 25% better than other concentrations, namely 20%, 15%, and 0%. This study gave a better effect on the use of POC than those without fertilizers due to the addition of nutrients provided to increase the growth of black soybean plants.Key words: Black Soybean, POC, Water Tofu, Water Coconut. 
Mitigation and Innovation in Making Natural Handwash Made from Coffee Grounds in Dusun X Batangkuis Deli Serdang Schools Towards Face-to-Face Schools Elimasni; Isnaini Nurwahyuni; Deny Supriharti; Rizky Yudha Pratama
ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat Vol. 8 No. 2 (2023): ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/abdimastalenta.v8i2.13932

Abstract

The government's desire to reopen and implement offline education has become a hot topic of discussion lately. The risk of spreading covid 19 to children is the concern of many people. For this reason, mitigation and prevention efforts need to be carried out to reduce the potential for the spread of covid 19. One effort that can be done is to make hand washing a good habit. The activity was carried out by providing training in making liquid hand soap for the community, especially teachers and school students in Dusun X to socialize how to prevent the spread of Covid-19 in schools. Activities are carried out through 5 (five) stages which include (1) Survey (2) Soap Making Test (3) Socialization; (4) Soap-making skills training; (5) Monitoring and Evaluation. It is hoped that the manufacture of natural handwash can be used for schools and school children in Dusun X Batangkuis Deliserdang to prevent covid 19 from going to face-to-face school in 2021.
Application of water treatment technology with help solar and antimicrobial power to village communities Tanjung Selamat Sub-district Sunggal Districts Deli Serdang Isnaini Nurwahyuni; Riyanto Sinaga; Rizky Yudha Pratama; Fathurrahman
ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat Vol. 4 No. 2 (2019): ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (756.264 KB) | DOI: 10.32734/abdimastalenta.v4i2.4184

Abstract

The need for clean water is a challenge in rural areas, especially villages that have not yet been reached by the Regional Water Company (Perusahaan Daerah Air Minum,PDAM). One of the villages experiencing problems with the availability of clean water is Tanjung Selamat Housing Village, Deli Serdang Regency. The people in this village rely solely on ground water that comes from private bore wells. The problem faced is that the water is relatively high in turbidity, and the level of water sterilization is also very low because it contains micro-organisms that cause disease such as dysentery, typhoid diarrhea and so on. An efforts to clean water using citrate have been made but the level of turbidity of the water is still above the threshold. To overcome this problem, appropriate technology is needed for water treatment to produce clean water. The purpose of this activity is to design and implement a technology to process raw water into clean water for public consumption. A design of antimicrobial water purification set with the help of solar power has been provided in Tanjung Selamat Deli Serdang Housing in 2019. The steps taken are surveying the existence of water standards, microbial laboratory testing and acidity test. Low quality raw water has obtained in the target villagewhere the water is high turbidity level, yellowish color, acidity level 6, 23 and contains coliform E.coli bacteria, so it requires processing before it is used as raw material for domestic purposes and drinking water. The application of purifying technology with the help of solar panels is able to convert raw water into clear water, has an acidity of 8.6 and is free of E. coli bacteria. The water produced is also alkaline so it is good for domestic consumption. Water user communities really feel the benefits of appropriate water purification technology developed in community service activities
Quality of Effective Microorganisms-based Liquid Fertilizer from Fermented Papaya Fruits (Carica papaya L.) Simarmata, Wilander; Yurnaliza, Yurnaliza; Nurwahyuni, Isnaini
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 8, No 2: Jurnal Pembelajaran Dan Biologi Nukleus Juli 2022
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v8i2.2733

Abstract

Papaya (Carica papaya L.) is one of the most popular fruits in the community and is generally only used as an ingredient for consumption in a fresh state. Papaya fruit production tends to be dynamic where sometimes the abundant amount of fruit may not necessarily be marketed directly. This causes the stock of papaya fruit during storage to rot and cannot be reused. This study aims to explore the potential of ripe papaya fruit to be processed as a substrate for the manufacture of liquid fertilizer using local microorganisms or effective microorganisms (EM). The quality of EM-based liquid fertilizer was determined based on the bacterial population density (CFU/mL) using the standard plate count (SPC) method on different growth media, then the levels of IAA and NPK in the applied fermented papaya fruit. The results showed the occurrence of nitrogen fixing bacteria, phosphate solubilizing bacteria and chitinolytic bacteria besides the cosmopolite bacteria. The optimum level of IAA in the fermented papaya fruit was EM + molasses (100%) while the NPK profile was still higher in the EM treatment without molasses.
Plant Effectiveness of Acorus calamus, Pistia stratiotes, Azolla pinnata as Hyperaccumulator Candidate of Phytoremediation Agent for Copper (Cu) Absorption Dewi, Juriyah Astika; Nurwahyuni, Isnaini; Munir, Erman
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4291

Abstract

Metals are harmful pollutants because they cannot be broken down by living organisms. An excess of metal can become toxic. The metal in question is copper (Cu). This research aimed to determine the phytoremediation effectiveness of Acorus calamus, Pistia stratiotes, and Azolla pinnata as candidates for Cu metal absorption. This investigation employed a wholly random factorial design with two factors: plant absorbent materials comprised of three plant species (Acorus calamus, Pistia stratiotes, and Azolla pinnata) and absorption concentrations of 2 and 5 ppm. The ANOVA test was used to analyze the data, followed by the average difference test. According to the results of this study, three plants—Acorus calamus, Pistia stratiotes, and Azolla pinnata—are capable of reducing Cu metal in water. The Acorus calamus plant can reduce Cu concentrations in water from 2 ppm to 96.85% and from 5 ppm to 96.80%. Pistia stratiotes plants can reduce Cu level in water from 2 ppm to 96.50% and 5 ppm to 99.94% at concentrations of 2 and 5 ppm, respectively. The Azolla pinnata plant can reduce Cu level in water from 2 ppm to 98.50% and from 5 ppm to 96.54% at concentrations of 2 ppm and 5 ppm, respectively. The highest BCF value at a concentration of 2 ppm is in the roots of Azolla pinnata plants, at 6.77 mg/kg, followed by the leaves, at 8.88 mg/kg. The maximum BCF value at a concentration of 5 ppm for Pistia stratiotes plants is 2.26 mg/kg for the roots and 2.46 mg/kg for the foliage. The greatest concentration of TF at 2 ppm in Azolla pinnata is 1.31 mg/kg, while the maximum concentration at 5 ppm in Acorus calamus is 1.98 mg/kg
Co-Authors . Rosmayati ., Rosmayati Andini - Hanif Arifda Ayu Aththorick, T Alief Deny Supriharti Dessy Merry Silitonga Dewi, Juriyah Astika DWI SURYANTO Elimasni Elimasni Erman Munir Erman Munir Erni Jumilawaty Etti Sartina Siregar Fathurrahman Fathurrahman Fathurrahman Fathurrahman Fauziah Harahap Fauziah Harahap Hasianna Nopina Situmorang Hesti Wahyuningsih Julius Paolo Siregar Justin A. Napitupulu Kaniwa Berliani KIKI NURTJAHJA manihar Situmorang, manihar Masitta Tanjung Mayang Sari Yeanny Mizawarti Mizawarti Napitupulu, Justin A NUNUK PRIYANI Putri Amelia Lubis Radite Tistama Rahma Dina Rani Ernita Limbong Rizky Yudha Pratama Rizky Yudha Pratama Rizky Yudha Pratama Rizky Yudha Pratama Safira Mustaqilla Silitonga, P Maulim Simarmata, Wilander Sinaga, Rianto Sinaga, Riyanto Siregar, Linda Sari Steven Taniwan Vahnoni Lubis Yurnaliza, Yurnaliza Zulfatma Amanda