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Potensi Bakteri Endofit Pelarut Fosfat dan Penghasil IAA pada Pertumbuhan Tanaman Cabai Zulfatma Amanda; Erman Munir; Isnaini Nurwahyuni
Jurnal Penelitian Pendidikan IPA Vol 10 No 10 (2024): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i10.8297

Abstract

Biofertilizer is considered an alternative to chemical fertilizers for enhancing plant productivity while minimizing ecological damage. Endophytic bacteria have the ability to solubilize phosphate and produce IAA (indole acetic acid). The aim of this research is to assess the phosphate solubilization and IAA production abilities of bacterial isolates applied to chili plants. Seven bacterial isolates collection from the Microbiology Laboratory, Universitas Sumatera Utara, were tested. In quantitative tests conducted in vitro, isolates MB10 and MB11 demonstrated the highest phosphate solubilization capabilities, yielding solubilization indices of 16.2 mm and 9.5 mm, respectively. Additionally, the ability of bacterial isolates to produce IAA in vitro was evaluated using calorimetry, revealing that isolates MB8 and MB2 produced the highest concentrations of IAA, measuring 58.58 ppm and 54.64 ppm, respectively. Furthermore, in vivo application involved the inoculation of isolates MB10 and MB11 on chili plants for 30 days. Seed treatment with suspensions of isolates MB10 and MB11 resulted in decreased plant performance compared to the control. These findings indicate that some endophytic bacteria originating from the mangrove root Avicennia marina have potential for producing relatively high levels of secondary metabolites. However, direct application did not significantly enhance plant growth performance.
Secondary Metabolites and Antioxidant Properties of Lichens from Sicike-Cike Nature Park, North Sumatra Putri Amelia Lubis; Etti Sartina Siregar; Isnaini Nurwahyuni
Jurnal Penelitian Pendidikan IPA Vol 11 No 4 (2025): April
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i4.11042

Abstract

This study aimed to evaluate the phytochemical composition and antioxidant activity of five lichen species Cladonia portentosa, Cladonia rapii, Letharia vulpina, Parmotrema hypotropum, and Usnea trichodea collected from Sicike-Cike Nature Park, North Sumatra, Indonesia. Methanol extracts were prepared from dried and powdered lichen samples. Qualitative phytochemical screening using specific reagents identified the presence of alkaloids, flavonoids, tannins, saponins, terpenoids/steroids, and glycosides in varying levels among the species. Antioxidant activity was assessed using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method, with IC50 values ranging from 42.3244 µg/mL to 86.7479 µg/mL. Parmotrema hypotropum demonstrated the strongest antioxidant activity (IC50 = 42.3244 µg/mL), categorized as moderate based on literature benchmarks. The strong activity is likely due to the presence of alkaloids, flavonoids, and saponins. In contrast, Usnea trichodea showed the weakest activity. These results suggest that certain lichens, especially Parmotrema hypotropum, may serve as promising sources of natural antioxidants. The findings support potential applications in pharmaceutical formulations and natural cosmetic products, particularly those targeting oxidative stress. Future studies should focus on isolating dominant compounds such as usnic acid or flavonoid derivatives and further exploring their bioactivity profiles.
Ethnobotanical Study of Medicinal Plants for the Circulatory System in Kaloy Village, Tamiang Hulu Subdistrict, Aceh Tamiang Regency Mustaqilla, Safira; Aththorick, T Alief; Nurwahyuni, Isnaini
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 3 (2025): September
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i3.17033

Abstract

This study aims to document the traditional knowledge of the local community in Kaloy Village, Tamiang Hulu Subdistrict, Aceh Tamiang Regency regarding the use of medicinal plants for treating circulatory system disorders. The methods used include in-depth interviews, direct observation, and documentation involving 90 respondents from various age groups and two key informants. The results show that the community's trust in medicinal plants remains high, particularly among individuals aged over 50. A total of 10 plant species were identified and used to treat diseases such as hypertension, high cholesterol, anemia, stroke, and diabetes. The most commonly utilized plant parts were leaves and rhizomes, with traditional processing methods such as boiling, pounding, and chewing. The study also calculated the Index of Cultural Significance (ICS) to assess the cultural importance of each species in the community. These findings indicate that ethnobotanical knowledge is still well preserved among the older generation but is beginning to decline among the youth.
Information Boards as Forest Conservation Campaign in Bahorok Research Center, Langkat Regency, North Sumatera Utara, Indonesia Kaniwa Berliani; Elimasni; Erni Jumilawaty; Kiki Nurtjahja; Hesti Wahyuningsih; Etti Sartina Siregar; Isnaini Nurwahyuni; Masitta Tanjung; Nunuk Priyani; Deny Supriharti; Julius Paolo Siregar
ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat Vol. 9 No. 2 (2024): ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/abdimastalenta.v9i2.16643

Abstract

Yayasan Ekosistem Lestari (YEL) in Bahorok Research Station in Bahorok Sub-district, Langkat District, is a very important location for this work. The hilly terrain of the area provides a beautiful landscape and varied flora and fauna with the main attraction being the corpse flower (Amorphophallus sp). With a preserved natural environment and unique ecological features, this research station allows other parties to participate in protecting the area in various conservation campaign, one of which is the installation/establishment of conservation information boards. Conservation information boards can raise public awareness about the importance of protecting forests and their natural environment. Information on ecosystems, flora, fauna, management, and the importance of conservation can be conveyed through these information boards. Information boards are widely used as an educational tool for local communities and visitors to the forest at the research station. They can learn about forest ecology, the negative impacts of deforestation, and sustainable ways of utilizing forest resources. They can learn about forest ecology, the negative impacts of deforestation, and sustainable ways of utilizing forest resources. Information boards can also be used to invite community participation in conservation efforts, which is expected to improve the quality of knowledge and understanding of conservation areas for communities, researchers, and tourists. Therefore, the installation/establishment of conservation information boards needs to be carried out at Bahorok Research Station, Batu Katak as one of the campaign and solutions to maintain the natural conditions in the forest and river.