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Formulasi dan Evaluasi Sediaan Mikroemulsi Untuk Penghantaran Transdermal Ketoprofen dengan Fasa Minyak Labrafil M1944CS Priani, Sani Ega; Darijanto, Sasanti Tarini; Suciati, Tri; Iwo, Maria Immaculata
Jurnal Matematika dan Sains Vol 19 No 3 (2014)
Publisher : Institut Teknologi Bandung

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Ketoprofen termasuk obat anti inflamasi non steroid (NSAIDs) untuk pengobatan simptomatik nyeri dan inflamasi. Pemakaian ketoprofen secara transdermal diketahui mampu menghantarkan zat aktif untuk mencapai konsentrasi efektif pada jaringan target, dengan konsentrasi plasma yang lebih rendah dibanding penggunaan per oral, sehingga mengurangi resiko efek samping sistemik. Penelitian ini bertujuan untuk mendapatkan mikroemulsi ketoprofen yang stabil secara fisik untuk penghantaran transdermal. Optimasi formula mikroemulsi dibuat menggunakan fasa minyak labrafil M 1944 CS, surfaktan Cremophor EL, serta kosurfaktan etanol, propilenglikol, dan gliserin. Evaluasi sediaan meliputi pengamatan organoleptik, pH, viskositas, dan ukuran globul, serta pengujian stabilitas fisik menggunakan metode sentrifugasi dan freeze thaw. Selanjutnya dilakukan uji difusi in vitro dan uji iritasi kulit dan mata pada kelinci. Formula mikroemulsi optimum mengandung labrafil 15%, cremophor EL 30%, dan propilenglikol 10%. Sediaan mikroemulsi memenuhi kriteria stabilitas fisik berdasarkan uji sentrifugasi dan freeze thaw. Nilai pH dan viskositas sediaan relatif stabil selama 120 hari penyimpanan pada suhu kamar. Mikroemulsi ketoprofen memiliki ukuran globul rata-rata 29,3 nm. Jumlah ketoprofen terdifusi selama 180 menit pengujian adalah 386,6 ± 61,2 µg/cm2. Sediaan mikroemulsi mengiritasi ringan pada kulit tetapi tidak mengiritasi mata. Kata kunci: Ketoprofen, Mikroemulsi, Transdermal, Difusi. Formulation and Evaluation of Microemulsion for Ketoprofen Transdermal Delivery Using Labrafil M1944CS as an Oil Phase Abstract Ketoprofen belongs to NSAIDs and is commonly applied for symptomatic treatment of pain and inflamation. Transdermal route of ketoprofen application enables the delivery of this active substance to reach its effective concentration in target organ but with lower plasma concentration compared to that of per oral application and hence can reduce systemic side effects. The objectives of this study are to obtain microemulsion formulation of ketoprofen for transdermal delivery. Microemulsion was formulated using Labrafil M 1944 CS as an oil phase, cremophor EL as surfactant and etanol, propylene glycol, glycerin as cosurfactans. Evaluation of ketoprofen microemulsion included organoleptic evaluation, pH, viscocity, globul diameter, and physical stability test using centrifugation and freeze thaw methods. Skin permeation was evaluated in vitro using spangler membrane and irritation effect test on rabbits. The optimum formulation of microemulsion was labrafil 15%, cremophor EL 30%,and propylene glycol 10%. Microemulsion of ketoprofen did not show any changes during freeze thaw and centrifugation tests which indicated its stability. The viscosity and pH of preparations were relatively stable for 120 days storage at room temperature. Average globule diameter of microemulsion was 29.3 nm. The total ketoprofen diffused was 386.6 ± 61.2 µg/cm2 for 180 minutes of testing time. The microemulsion showed slight irritation effect on the skin but no irritation effect on the eyes. Keywords : Ketoprofen, Microemulsion, Transdermal, Diffusion.

Pengembangan, Evaluasi, dan Uji Aktivitas Antiinflamasi Akut Sediaan Nanoemulsi Spontan Minyak Jintan Hitam Olii, Audia Triani; Pamudji, Jessie Sofia; Mudhakir, Diky; Iwo, Maria Immaculata
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 7, No 2 (2014)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar

Nigela sativa is the plant in which the seeds have a strong anti inflammatory effect. The compound that responsible to perform anti inflammation effect is thimoquinon, that contained in volatile constituents of Nigella sativa oil. Later it was found that even the fixed oil of Nigella sativa has greater anti inflammation effect than thimoquinon. Limited usage of oil or powder have a problems in bioavailability. To overcome this problem, the self nanoemulsion formula development is done to enhance the bioavailability. Nanoemulsion contain 200μL Nigella sativa oil per 1 g mixture of oil phase made by VCO, Cremophor RH40, polietilen glikol 400 rasio 1:7:2. Disper sibility test showed that SNEDDS is rapidly form a transparent mixture within 9,17 ± 0,015 minutes in HCl 0,1 N, the rate of stirage at 50 rpm, mean globules diameter of 57,6 ± 13.04 nm, polydispersity index of 0,288 ± 0,033, and zeta potential of 0,243 ± 0,136 mV. Stability test of centrifuge test showed that SNEDDS is stable, although at the third cycle of freeze thaw cycle showing a significant difference in polydispersity index and zeta potensial with the previous cycle. Result of anti inflammation acute activity test of Nigella sativa oil which is incorporate in SNEDDS of VCO has no antiinflammation. Keywords: Nigella sativa oil, Self Nanoemulsion, anti inflammation Nigella sativa, tanaman dengan biji yang memiliki efek antiinflamasi yang cukup kuat. Senyawa yang bertanggung jawab memberikan efek antiinflamasi adalah thimokuinon, senyawa yang terkandung dalam minyak menguap dari minyak jintan hitam. Namun ditemukan bahwa bahkan minyak lemak jintan hitam memiliki aktivitas antiinflamasi yang lebih besar dari thimokuinon. Penggunaan yang terbatas pada minyak atau serbuk jintan hitam mempunyai masalah dalam bioavailabilitas. Untuk mengatasi masalah ini, maka dilakukan pengembangan formula sediaan nanoemulsi spontan (SNES) dalam usaha meningkatkan bioavailabilitas minyak jintan hitam. Nanoemulsi yang mengandung 200 μL minyak jintan hitam per 1 g fase minyak dibuat dengan menggunakan VCO, Cremophor RH 40 dan polietilen glikol 400 dengan perbandingan 1:7:2. Hasil uji dispersibilitas menunjukkan sediaan teremulsi dengan cepat membentuk campuran transparan dalam waktu 9,17 ± 0,015 menit dalam medium HCl 0,1 N dengan kecepatan pengadukan 50 rpm, dengan ukuran diameter globul 57,6 ± 13.04 nm, indeks polidispersitas 0,288 ± 0,033, dan zeta potensial 0,243 ± 0,136 mV. Hasil uji stabilitas pada uji sentrifugasi menunjukkan SNES minyak jintan hitam stabil secara fisik, walaupun pada siklus ketiga freeze thawmenunjukkan perbedaan yang signifikan dengan siklus sebelumnya pada indeks polidispersi dan zeta potensial. Hasil uji aktivitas antiinflamasi akut minyak jintan hitam yang diinkorporasikan dalam SNES VCO tidak memberikan efek antiinflamasi. Kata kunci: minyak jintan hitam, nanoemulsi spontan, antiinflamasi akut

Pengembangan, Evaluasi, dan Uji Aktivitas Antiinflamasi Akut Sediaan Nanoemulsi Spontan Minyak Jintan Hitam Olii, Audia Triani; Pamudji, Jessie Sofia; Mudhakir, Diky; Iwo, Maria Immaculata
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 7, No 2 (2014)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar

Nigela sativa is the plant in which the seeds have a strong anti inflammatory effect. The compound that responsible to perform anti inflammation effect is thimoquinon, that contained in volatile constituents of Nigella sativa oil. Later it was found that even the fixed oil of Nigella sativa has greater anti inflammation ef-fect than thimoquinon. Limited usage of oil or powder have a problems in bioavailability. To overcome this problem, the self nanoemulsion formula development is done to enhance the bioavailability. Nanoemulsion contain 200μL Nigella sativa oil per 1 g mixture of oil phase made by VCO, Cremophor RH40, polietilen glikol 400 rasio 1:7:2. Disper sibility test showed that SNEDDS is rapidly form a transparent mixture within 9,17 ± 0,015 minutes in HCl 0,1 N, the rate of stirage at 50 rpm, mean globules diameter of 57,6 ± 13.04 nm, polydispersity index of 0,288 ± 0,033, and zeta potential of 0,243 ± 0,136 mV. Stability test of centrifuge test showed that SNEDDS is stable, although at the third cycle of freeze thaw cycle showing a significant difference in polydispersity index and zeta potensial with the previous cycle. Result of anti inflammation acute activity test of Nigella sativa oil which is incorporate in SNEDDS of VCO has no antiinflammation. Keywords: Nigella sativa oil, Self Nanoemulsion, anti inϔlammation Nigella sativa, tanaman dengan biji yang memiliki efek antiinflamsi yang cukup kuat. Senyawa yang bertanggung jawab memberikan efek antiinflamasi adalah thimokuinon, senyawa yang terkandung dalam minyak menguap dari minyak jintan hitam. Namun ditemukan bahwa bahkan minyak lemak jintan hitam memiliki aktivitas antiinflamasi yang lebih besar dari thimokuinon. Penggunaan yang terbatas pada minyak atau serbuk jintan hitam mempunyai masalah dalam bioavailabilitas. Untuk mengatasi masalah ini, maka dilakukan pengembangan formula sediaan nanoemulsi spontan (SNES) dalam usaha meningkatkan bioavailabilitas minyak jintan hitam. Nanoemulsi yang mengandung 200 μL minyak jintan hitam per 1 g fase minyak dibuat dengan menggunakan VCO, Cremophor RH 40 dan polietilen glikol 400 dengan perbandingan 1:7:2. Hasil uji dispersibilitas menunjukkan sediaan teremulsi dengan cepat membentuk campuran transparan dalam waktu 9,17 ± 0,015 menit dalam medium HCl 0,1 N dengan kecepatan pengadukan 50 rpm, dengan ukuran diameter globul 57,6 ± 13.04 nm, indeks polidispersitas 0,288 ± 0,033, dan zeta potensial 0,243 ± 0,136 mV. Hasil uji stabilitas pada uji sentrifugasi menunjukkan SNES minyak jintan hitam stabil secara fisik, walaupun pada siklus ketiga freeze thaw menunjukkan perbedaan yang signifikan dengan siklus sebelumnya pada indeks polidispersi dan zeta potensial. Hasil uji aktivitas antiinflamasi akut minyak jintan hitam yang diinkorporasikan dalam SNES VCO tidak memberikan efek antiinflamasi.Kata kunci: minyak jintan hitam, nanoemulsi spontan, antiinϐlamasi akut

Pengembangan, Evaluasi, dan Uji Aktivitas Antiinflamasi Akut Sediaan Nanoemulsi Spontan Minyak Jintan Hitam Olii, Audia Triani; Pamudji, Jessie Sofia; Mudhakir, Diky; Iwo, Maria Immaculata
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 7, No 2 (2014)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar

Nigela sativa is the plant in which the seeds have a strong anti inflammatory effect. The compound that responsible to perform anti inflammation effect is thimoquinon, that contained in volatile constituents of Nigella sativa oil. Later it was found that even the fixed oil of Nigella sativa has greater anti inflammation ef-fect than thimoquinon. Limited usage of oil or powder have a problems in bioavailability. To overcome this problem, the self nanoemulsion formula development is done to enhance the bioavailability. Nanoemulsion contain 200μL Nigella sativa oil per 1 g mixture of oil phase made by VCO, Cremophor RH40, polietilen glikol 400 rasio 1:7:2. Disper sibility test showed that SNEDDS is rapidly form a transparent mixture within 9,17 ± 0,015 minutes in HCl 0,1 N, the rate of stirage at 50 rpm, mean globules diameter of 57,6 ± 13.04 nm, polydispersity index of 0,288 ± 0,033, and zeta potential of 0,243 ± 0,136 mV. Stability test of centrifuge test showed that SNEDDS is stable, although at the third cycle of freeze thaw cycle showing a significant difference in polydispersity index and zeta potensial with the previous cycle. Result of anti inflammation acute activity test of Nigella sativa oil which is incorporate in SNEDDS of VCO has no antiinflammation. Keywords: Nigella sativa oil, Self Nanoemulsion, anti inϔlammation Nigella sativa, tanaman dengan biji yang memiliki efek antiinflamsi yang cukup kuat. Senyawa yang bertanggung jawab memberikan efek antiinflamasi adalah thimokuinon, senyawa yang terkandung dalam minyak menguap dari minyak jintan hitam. Namun ditemukan bahwa bahkan minyak lemak jintan hitam memiliki aktivitas antiinflamasi yang lebih besar dari thimokuinon. Penggunaan yang terbatas pada minyak atau serbuk jintan hitam mempunyai masalah dalam bioavailabilitas. Untuk mengatasi masalah ini, maka dilakukan pengembangan formula sediaan nanoemulsi spontan (SNES) dalam usaha meningkatkan bioavailabilitas minyak jintan hitam. Nanoemulsi yang mengandung 200 μL minyak jintan hitam per 1 g fase minyak dibuat dengan menggunakan VCO, Cremophor RH 40 dan polietilen glikol 400 dengan perbandingan 1:7:2. Hasil uji dispersibilitas menunjukkan sediaan teremulsi dengan cepat membentuk campuran transparan dalam waktu 9,17 ± 0,015 menit dalam medium HCl 0,1 N dengan kecepatan pengadukan 50 rpm, dengan ukuran diameter globul 57,6 ± 13.04 nm, indeks polidispersitas 0,288 ± 0,033, dan zeta potensial 0,243 ± 0,136 mV. Hasil uji stabilitas pada uji sentrifugasi menunjukkan SNES minyak jintan hitam stabil secara fisik, walaupun pada siklus ketiga freeze thaw menunjukkan perbedaan yang signifikan dengan siklus sebelumnya pada indeks polidispersi dan zeta potensial. Hasil uji aktivitas antiinflamasi akut minyak jintan hitam yang diinkorporasikan dalam SNES VCO tidak memberikan efek antiinflamasi.Kata kunci: minyak jintan hitam, nanoemulsi spontan, antiinϐlamasi akut

Pengembangan, Evaluasi, dan Uji Aktivitas Antiinflamasi Akut Sediaan Nanoemulsi Spontan Minyak Jintan Hitam Olii, Audia Triani; Pamudji, Jessie Sofia; Mudhakir, Diky; Iwo, Maria Immaculata
Jurnal Farmasi Indonesia Vol 7, No 2 (2014)
Publisher : Jurnal Farmasi Indonesia

Nigela sativa is the plant in which the seeds have a strong anti inflammatory effect. The compound that responsible to perform anti inflammation effectÂ is thimoquinon, that contained in volatile constituents of Nigella sativa oil. Later itÂ was found that even the fixed oil of Nigella sativa has greater anti inflammation ef-fect than thimoquinon. Limited usage of oil or powder have a problems in bioavailability. To overcome this problem, the self nanoemulsion formula development isÂ done to enhance the bioavailability. Nanoemulsion contain 200Î¼L Nigella sativa oilÂ per 1 g mixture of oil phase made by VCO, Cremophor RH40, Â polietilen glikol 400Â rasio 1:7:2. Disper sibility test showed that SNEDDS is rapidly form a transparentÂ mixture within 9,17 Â± 0,015 minutes in HCl 0,1 N, the rate of stirage at 50 rpm, meanÂ globules diameter of 57,6 Â± 13.04 nm, polydispersity index of 0,288 Â± 0,033, and zetaÂ potential of 0,243 Â± 0,136 mV. Stability test of centrifuge test showed that SNEDDSÂ is stable, although at the third cycle of freeze thaw cycle showing a significant difference in polydispersity index and zeta potensial with the previous cycle. Result ofÂ anti inflammation acute activity test of Nigella sativa oil which is incorporate inÂ SNEDDS of VCO has no antiinflammation. Keywords: Â Nigella sativa oil, Self Nanoemulsion, anti inÏ?lammation Â Nigella sativa, tanaman dengan biji yang memiliki efek antiinflamsi yang cukup kuat. Senyawa yang bertanggung jawab memberikan Â efek antiinflamasi adalah thimokuinon, senyawa yang terkandung dalam minyak Â menguap dari minyak jintan hitam. Namun ditemukan bahwa Â bahkan minyak lemak jintan hitam memiliki aktivitas antiinflamasi yang lebih besar dari thimokuinon. Penggunaan yang terbatas pada minyak atau serbuk jintan hitam mempunyai Â masalah dalam bioavailabilitas. Untuk mengatasi masalah ini, maka dilakukan pengembangan formula sediaan nanoemulsi Â spontan (SNES) dalam usaha meningkatkan bioavailabilitas minyak jintan hitam. Nanoemulsi yang mengandung 200 Î¼L minyak jintan hitam per 1 g fase minyak dibuat dengan menggunakan VCO, Cremophor RH 40 dan polietilen glikol 400 dengan perbandingan 1:7:2. Hasil uji dispersibilitas menunjukkan sediaan teremulsi dengan cepat membentuk campuran transparan dalam waktu 9,17 Â± 0,015 menit dalam medium HCl 0,1 N dengan kecepatan pengadukan 50 rpm, dengan ukuran diameter globul 57,6 Â± 13.04 nm, indeks polidispersitas Â 0,288 Â± 0,033, Â dan zeta potensial 0,243 Â± 0,136 mV. Hasil uji stabilitas pada uji sentrifugasi menunjukkan SNES minyak jintan hitam stabil secara fisik, walaupun pada siklus ketiga freeze thaw menunjukkan perbedaan yang signifikan dengan siklus sebelumnya pada indeks polidispersi dan zeta potensial. Hasil uji aktivitas antiinflamasi akut minyak jintan hitam yang diinkorporasikan dalam SNES VCO tidak memberikan efek antiinflamasi.Kata kunci: minyak jintan hitam, nanoemulsi spontan, antiinÏlamasi akut Â

Pengembangan, Evaluasi, dan Uji Aktivitas Antiinflamasi Akut Sediaan Nanoemulsi Spontan Minyak Jintan Hitam Olii, Audia Triani; Pamudji, Jessie Sofia; Mudhakir, Diky; Iwo, Maria Immaculata
Jurnal Farmasi Indonesia Vol 7, No 2 (2014)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v7i2.166

Nigela sativa is the plant in which the seeds have a strong anti inflammatory effect. The compound that responsible to perform anti inflammation effectÂ is thimoquinon, that contained in volatile constituents of Nigella sativa oil. Later itÂ was found that even the fixed oil of Nigella sativa has greater anti inflammation effect than thimoquinon. Limited usage of oil or powder have a problems in bioavailability. To overcome this problem, the self nanoemulsion formula development isÂ done to enhance the bioavailability. Nanoemulsion contain 200Î¼L Nigella sativa oilÂ per 1 g mixture of oil phase made by VCO, Cremophor RH40, Â polietilen glikol 400Â rasio 1:7:2. Disper sibility test showed that SNEDDS is rapidly form a transparentÂ mixture within 9,17 Â± 0,015 minutes in HCl 0,1 N, the rate of stirage at 50 rpm, meanÂ globules diameter of 57,6 Â± 13.04 nm, polydispersity index of 0,288 Â± 0,033, and zetaÂ potential of 0,243 Â± 0,136 mV. Stability test of centrifuge test showed that SNEDDSÂ is stable, although at the third cycle of freeze thaw cycle showing a significant difference in polydispersity index and zeta potensial with the previous cycle. Result ofÂ anti inflammation acute activity test of Nigella sativa oil which is incorporate inÂ SNEDDS of VCO has no antiinflammation. Keywords: Nigella sativa oil, Self Nanoemulsion, anti inflammation Â Nigella sativa, tanaman dengan biji yang memiliki efek antiinflamasi yang cukup kuat. Senyawa yang bertanggung jawab memberikan Â efek antiinflamasi adalah thimokuinon, senyawa yang terkandung dalam minyak Â menguap dari minyak jintan hitam. Namun ditemukan bahwa Â bahkan minyak lemak jintan hitam memiliki aktivitas antiinflamasi yang lebih besar dari thimokuinon. Penggunaan yang terbatas pada minyak atau serbuk jintan hitam mempunyai Â masalah dalam bioavailabilitas. Untuk mengatasi masalah ini, maka dilakukan pengembangan formula sediaan nanoemulsi Â spontan (SNES) dalam usaha meningkatkan bioavailabilitas minyak jintan hitam. Nanoemulsi yang mengandung 200 Î¼L minyak jintan hitam per 1 g fase minyak dibuat dengan menggunakan VCO, Cremophor RH 40 dan polietilen glikol 400 dengan perbandingan 1:7:2. Hasil uji dispersibilitas menunjukkan sediaan teremulsi dengan cepat membentuk campuran transparan dalam waktu 9,17 Â± 0,015 menit dalam medium HCl 0,1 N dengan kecepatan pengadukan 50 rpm, dengan ukuran diameter globul 57,6 Â± 13.04 nm, indeks polidispersitas Â 0,288 Â± 0,033, Â dan zeta potensial 0,243 Â± 0,136 mV. Hasil uji stabilitas pada uji sentrifugasi menunjukkan SNES minyak jintan hitam stabil secara fisik, walaupun pada siklus ketigaÂ freeze thawmenunjukkan perbedaan yang signifikan dengan siklus sebelumnya pada indeks polidispersi dan zeta potensial. Hasil uji aktivitas antiinflamasi akut minyak jintan hitam yang diinkorporasikan dalam SNES VCO tidak memberikan efek antiinflamasi. Kata kunci: minyak jintan hitam, nanoemulsi spontan, antiinflamasi akut Â Â

Pengembangan, Evaluasi, dan Uji Aktivitas Antiinflamasi Akut Sediaan Nanoemulsi Spontan Minyak Jintan Hitam Olii, Audia Triani; Pamudji, Jessie Sofia; Mudhakir, Diky; Iwo, Maria Immaculata
Jurnal Farmasi Indonesia Vol 7, No 2 (2014)
Publisher : Jurnal Farmasi Indonesia

Nigela sativa is the plant in which the seeds have a strong anti inflammatory effect. The compound that responsible to perform anti inflammation effectÂ is thimoquinon, that contained in volatile constituents of Nigella sativa oil. Later itÂ was found that even the fixed oil of Nigella sativa has greater anti inflammation ef-fect than thimoquinon. Limited usage of oil or powder have a problems in bioavailability. To overcome this problem, the self nanoemulsion formula development isÂ done to enhance the bioavailability. Nanoemulsion contain 200Î¼L Nigella sativa oilÂ per 1 g mixture of oil phase made by VCO, Cremophor RH40, Â polietilen glikol 400Â rasio 1:7:2. Disper sibility test showed that SNEDDS is rapidly form a transparentÂ mixture within 9,17 Â± 0,015 minutes in HCl 0,1 N, the rate of stirage at 50 rpm, meanÂ globules diameter of 57,6 Â± 13.04 nm, polydispersity index of 0,288 Â± 0,033, and zetaÂ potential of 0,243 Â± 0,136 mV. Stability test of centrifuge test showed that SNEDDSÂ is stable, although at the third cycle of freeze thaw cycle showing a significant difference in polydispersity index and zeta potensial with the previous cycle. Result ofÂ anti inflammation acute activity test of Nigella sativa oil which is incorporate inÂ SNEDDS of VCO has no antiinflammation. Keywords: Â Nigella sativa oil, Self Nanoemulsion, anti inÏ?lammation Â Nigella sativa, tanaman dengan biji yang memiliki efek antiinflamsi yang cukup kuat. Senyawa yang bertanggung jawab memberikan Â efek antiinflamasi adalah thimokuinon, senyawa yang terkandung dalam minyak Â menguap dari minyak jintan hitam. Namun ditemukan bahwa Â bahkan minyak lemak jintan hitam memiliki aktivitas antiinflamasi yang lebih besar dari thimokuinon. Penggunaan yang terbatas pada minyak atau serbuk jintan hitam mempunyai Â masalah dalam bioavailabilitas. Untuk mengatasi masalah ini, maka dilakukan pengembangan formula sediaan nanoemulsi Â spontan (SNES) dalam usaha meningkatkan bioavailabilitas minyak jintan hitam. Nanoemulsi yang mengandung 200 Î¼L minyak jintan hitam per 1 g fase minyak dibuat dengan menggunakan VCO, Cremophor RH 40 dan polietilen glikol 400 dengan perbandingan 1:7:2. Hasil uji dispersibilitas menunjukkan sediaan teremulsi dengan cepat membentuk campuran transparan dalam waktu 9,17 Â± 0,015 menit dalam medium HCl 0,1 N dengan kecepatan pengadukan 50 rpm, dengan ukuran diameter globul 57,6 Â± 13.04 nm, indeks polidispersitas Â 0,288 Â± 0,033, Â dan zeta potensial 0,243 Â± 0,136 mV. Hasil uji stabilitas pada uji sentrifugasi menunjukkan SNES minyak jintan hitam stabil secara fisik, walaupun pada siklus ketiga freeze thaw menunjukkan perbedaan yang signifikan dengan siklus sebelumnya pada indeks polidispersi dan zeta potensial. Hasil uji aktivitas antiinflamasi akut minyak jintan hitam yang diinkorporasikan dalam SNES VCO tidak memberikan efek antiinflamasi.Kata kunci: minyak jintan hitam, nanoemulsi spontan, antiinÏlamasi akut Â

PHYTOCHEMICAL, USE IN ETHNOMEDICINE, AND THERAPEUTIC ACTIVITIES OF MARCHANTIA GENUS Purkon, Dicki Bakhtiar; Fadhlillah, Faizah Min; Maigoda, Tonny Cortis; Iwo, Maria Immaculata; Soemardji, Adreanus Andaja; Nadhifah, Ainun; Sudaryat, Yayat
Journal of Vocational Health Studies Vol. 5 No. 3 (2022): March 2022 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V5.I3.2022.174-185

Background: Liverworts are a group of plants from the Marchantia genus containing various biologically active compounds and comprised of 65 species worldwide. This plant species belongs to the Marchantiaceae family, which was used empirically in China, North America, Ancient Greece, and Indonesia to treat open wounds, burns, hepatotoxicity, and infection prevention. Now, liverworts have great potential as medicinal and nutraceutical products because of their phytochemical content and diversity of pharmacological activities. Purpose: This review focuses on secondary metabolites obtained from the thalloid and whole plant parts (herbs) and the compilation of their pharmacological activities, which are still poorly documented. Review: This review article obtained related information through extensive international journals (online) scientific databases and offline (books) related to all searches of the species of the genus Marchantia which included: phytochemical content (secondary metabolites) and various pharmacological activities which were collected and compared all results of literature studies from various aspects. Some of the secondary metabolites are Marchantin A, B, D dan E; Paleatin B; Perrottetin F, and Plagiochin E, which have various pharmacological activities. Various results regarding all the therapeutic properties of the genus Marchantia have been produced in the world such as: antibacterial, antifungal, antioxidant, cytotoxic activity, anti-inflammatory, cardiotonic, hepatoprotective, muscle relaxant, antiosteoporosis, and skin care. Conclusion: Therefore, the potential for the development of medicinal, supplementary and nutraceutical products from various Marchantia species is very large and broad for the future.

The Quality Of Life of Patients with Chronic Kidney Disease who Undergo Hemodialysis in RSUD Cilacap Pratiwi, Arik Dian Eka; Iwo, Maria Immaculata; Murwiningsih, Murwiningsih
Jurnal Kesehatan dr. Soebandi Vol 13 No 1 (2025): Jurnal Kesehatan dr. Soebandi
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jkds.v13i1.839

Chronic Kidney Disease (CKD) is one of the mayor health problems in the worldwide. The incidence of CKD continously increase every year, including in Indonesia. Hemodialysis (HD) is the most widely used process of kidney function replacement in Indonesia, including in RSUD Cilacap. This research aim is to study the quality of life (QoL) of CKD patients undergo hemodialysis in RSUD Cilacap using analytic observational design with cross sectional approach. The sampling was carried out using purposive sampling technique. The quality of life of patients was assessed based on WHOQoL-BREF questionnaire consisting of 4 domains i.e., physical, psychological, social relationships, and environment. Other aspects i.e., the CKD causes and therapy adherence were also analyzed. This research employs a descriptive design. The study conducted was univariate to ascertain the distribution of respondent characteristics, which was utilized to evaluate the variance in the quality of life domain among haemodialysis patients. Result showed that hypertension was the main causes of CKD at RSUD Cilacap (59%) beside diabetic mellitus (17%). Before HD, the blood pressure of all patients were in stage 1 hypertension condition with systole: 148.67±29.24 mmHg and dyastole: 80.10±5.47 mmHg. Result of WHOQoL-BREF questionnaire analyses, showed that none of the four domains meet the criteria of good QoL. Generally, based on this data, it can be said that the QoL of CKD patients at RSUD Cilacap were poor categories with score for all domains and a total score (48.96±7.95) below the normal rate. Therapy of hypertension condition of patients before HD with furosemide alone, furosemide+clonidine, and or furosemide+valsartan, could maintain the blood pressure of patients in target value i.e, <140/90 mmHg. Based on those data, it can be concluded that compliance of the patients are important in the improving of QoL of CKD patients who undergo HD in RSUD Cilacap..

Piper cubeba L. f. Nanoemulsion: Formulation and Effect on Antioxidant Activity in Rat's Hippocampus and Prefrontal Cortex Dwita, Lusi Putri; Iwo, Maria Immaculata; Mauludin, Rachmat; Elfahmi, Elfahmi
Borneo Journal of Pharmacy Vol. 8 No. 2 (2025): Borneo Journal of Pharmacy
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjop.v8i2.8720

The previous study showed that Piper cubeba extract increased brain antioxidant capacity, alleviating cognitive impairment in rats. However, brain-targeted drug delivery is often limited by the blood-brain barrier. One of the approaches to overcome this obstacle is nanocarrier. This study aimed to formulate a nanoemulsion (NE) of P. cubeba extract and determine the antioxidant activity in the brain. The research begins with the determination of excipients and the formula of NE. The formula was then tested for globule size, polydispersity index, thermodynamic stability, zeta potential, pH, and viscosity. The globule's shape was determined using transmission electron microscopy (TEM). The antioxidant activity was tested by catalase and lipid peroxidase activity in the brain after giving NE orally for seven days. Formulations of NE resulted in nano-size globules and were stable thermodynamically. The NE formula significantly increased catalase and inhibited lipid peroxidase activities in the Rat's hippocampus and prefrontal cortex. This study resulted in a stable formula and low-cost production of P. cubeba extract NE, which is active as the brain's antioxidant and is feasible to develop as a neuroprotective agent for various neurodegenerative diseases.

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