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All Journal CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES Jurnal Kimia (Journal of Chemistry) Buletin Udayana Mengabdi Jurnal Media Sains LIMNOTEK
I Wayan Suirta
Program Studi Kimia FMIPA Universitas Udayana, Jimbaran, Bali, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Earth & Planetary Sciences Education Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology

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PEMBUATAN VIRGIN COCONUT OIL DENGAN EKSTRAK JAMUR Aspergillus niger SERTA UJI ANTIBAKTERI VCO DENGAN BAKTERI Staphylococcus aureus. I W. Suirta; I. A. R. A. Asih
Jurnal Kimia (Journal of Chemistry) Vol. 17, No.1, Januari 2023
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JCHEM.2023.v17.i01.p05

Abstract

Telah dilakukan pembuatan virgin coconut oil (VCO) dengan ekstrak jamur Aspergillus niger serta uji antibakteri VCO dengan bakteri Staphylococcus aureus. Kualitas VCO ditentukan dengan uji kadar air, angka asam, angka penyabunan, angka iod, uji organoleptik, dan analisis GC-MS. Ekstraksi VCO tanpa menggunakan ekstrak jamur hanya mendapatkan VCO sebanyak 5,859 g. Penambahan ekstrak jamur A. niger 0,5% b/v menghasilkan VCO sebanyak 8,832 g, menunjukkan terjadi kenaikan yang sangat signifikan (p<0,05). Hasil uji kadar air, angka asam, angka penyabunan, dan angka iod masing-masing diperoleh: 0,1958; 0,2929; 5,0487; dan 0,2781, hasil ini sesuai dengan baku mutu VCO yang ditetapkan. Hasil uji organoleptik memberikan VCO yang tidak berwarna dan tidak berbau, dan hasil analisis GC-MS diperoleh kandungan asam lemak rantai medium dengan kandungan asam laurat sebagai komponen terbanyak. Hasil uji antibakteri terhadap bakteri Staphylococcus aureus menunjukkan kemampuan VCO dalam menghambat pertumbuhan bakteri dengan zona hambat 13,5 mm. Kata kunci: antibakteri, Aspergillus niger, Staphylococcus aureus, virgin coconut oil. ABSTRACT Preparation of virgin coconut oil (VCO) with Aspergillus niger fungi extract and the antibacterial test of the VCO with Staphylococcus aureus has been carried out. The quality test of the VCO included water content, acid number, saponification number, iodine value, organoleptic test, and GC-MS analysis. VCO extraction without using fungi extract only got 5.859 g of VCO. The addition of 0.5% w/v of A. niger fungi extracts produced 8.832 g of VCO, indicating a very significant increase (p <0.05). The water content, acid number, saponification number, and iodine value obtained were 0.158; 0.2929; 5.0487; and 0.2781 respectively, which met the VCO quality standard. The organoleptic test proved that the VCO was colourless and odourless. Meanwhile, the GC-MS analysis showed the content of medium-chain fatty acids with lauric acid as the largest component. The antibacterial test against Staphylococcus aureus indicated the ability of VCO to inhibit the growth of bacteria with an inhibition zone of 13.5 mm. Keywords: antibacterial, Aspergillus niger, Staphylococcus aureus, virgin coconut oil.
PEMANFAATAN KULIT PISANG HIJAU LUMUT (Musa x Paradisiaca L.) SEBAGAI BAHAN AKTIF MASKER PEEL OFF DAN SABUN MANDI ANTIBAKTERI W.S. Rita; I.W. Suirta; I.A.R.A. Asih; E. Sahara; S.R. Santi
Buletin Udayana Mengabdi Vol 22 No 2 (2023): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/BUM.2023.v22.i02.04

Abstract

Hijau Lumut banana peel (HLBP) is useful as an active ingredient in masks and antibacterial bath soap. The purpose of the service is to provide training on making peel-off masks and antibacterial bath soap to women in Wongaya Gede Village, Penebel, Tabanan. The mask was made by dissolving carboxymethyl cellulose (CMC) and gelatin with hot water to form a gel, then other ingredients were added such as propylene glycol as a moisturizer, vitamin E as a skin protectant, and HLBP extract. All ingredients were homogenized until a gel-like mask was formed. Soap was produced by mixing coconut oil (VCO), olive, castor, and milk with a base (NaOH). Glycerin and honey were then added to keep the skin soft. The training was held on Thursday, 11 August 2022. This training can help women in using banana peels as masks and antibacterial soaps, so they can be used for entrepreneurship. Keywords: Banana, Antibacterial, Mask, Soap, Wongaya Gede
IDENTIFIKASI DAN UJI AKTIVITAS ANTIBAKTERI EKTRAK ETANOL KULIT DAUN LIDAH BUAYA TERHADAP BAKTERI Eschericia coli DAN Staphylococcus aureus Wasudewa, K. M.; Suirta, I W.; Wahjuni, S.
Jurnal Kimia (Journal of Chemistry) Vol. 18, No.1, Januari 2024
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JCHEM.2024.v18.i01.p09

Abstract

Telah dilakukan penelitian untuk mengidentifikasi senyawa aktif Antibakteri dari kulit daun Aloe vera. Penelitian dilakukan dengan mengekstrak 200g sampel kering kulit daun lidah buaya dengan cara maserasi menggunakan pelarut etanol selama 24 jam, kemudian pelarutnya diuapkan dengan rotary vacuum evaporator. Ekstrak kental yang diperoleh selanjutnya dipisahkan menggunakan kromatografi kolom dengan fasa diam silika gel dan fasa gerak n-heksana, etil asetat, dan etanol. Fraksi yang diperoleh kemudian diuji aktivitas antibakteri nya terhadap bakteri Escherichia coli dan staphylococcus aureus. Fraksi yang paling aktif kemudian dimurnikan dengan kromatografi kolom dan dilakukan identifikasi senyawa aktifnya dengan GC-MS. Hasil uji diperoleh bahwa fraksi n-heksan memiliki aktivitas antibakteri terbaik yang ditunjukkan dengan nilai zona hambat sebesar 12 mm terhadap E.coli dan 5 mm terhadap S. aureus. Hasil analisis senyawa dengan menggunakan GC MS diperoleh senyawa seperti: 1-metildodesilamin; 4-[2-(fenilsulfanil)etil] piridin; pentanal ; difenilefrin ; p-hidroksinorefedrin dan 5-(2-aminopropil)-2-metilfenol Kata kunci: Aktivitas antibakteri, Escherichia coli, lidah buaya, maserasi, Staphylococcus aureus. ABSTRACT This research was carried out to identify the active anti-bacterial compounds from aloe vera leaf skin. The study was conducted by extracting 200 grams of dried Aloe vera leaves skin using the maceration method with ethanol for 24 hours, then the solvent was evaporated using the rotary vacuum evaporator. The viscous extract obtained was then separated by column chromatography with a stationary phase of silica gel and a mobile phase of n-hexane, ethyl acetate, and ethanol. The obtained fractions were tested for their anti-bacterial activities against Escherichia coli and Staphylococcus aureus bacteria. The most active fraction was purified by column chromatography, and then the active compound was identified by GC-MS. The results showed that the n-hexane fraction had the best anti-bacterial activity, with an inhibition zone of 12 mm against E.coli and 5 mm against S. aureus. The results of compound analysis using GC-MS obtained compounds are 1-methyl dodecyl amine, 4-[2-(phenyl sulfonyl)ethyl] pyridine, pentanal, p-hydroxynorephedrine, 5-(2-aminopropyl)-2-methylphenol, di-phenylephrine. Keywords: Aloe vera leaf, antibacterial activity, Escherichia coli, maceration, Staphylococcus aureus
EFEKTIVITAS EKSTRAK METANOL DAUN NANGKA (Artocarpus heterophyllus Lam.) SEBAGAI INHIBITOR ALAMI TERHADAP KOROSI PADA BAJA ST 37 DALAM MEDIA KOROSIF HCl Dwiyanti, D. A. B.; Suarya, P.; Suirta, I W.
Jurnal Kimia (Journal of Chemistry) Vol. 17, No.2, Juli 2023
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JCHEM.2023.v17.i02.p11

Abstract

Korosi merupakan salah satu masalah lingkungan yang sering ditemukan dan juga menjadi pusat perhatian masyarakat khususnya di bidang industri besi dan baja. Untuk melindungi baja dari serangan korosi, maka perlu adanya perlindungan dari inhibitor. Inhibitor korosi adalah bahan kimia yang ditambahkan ke dalam lingkungan korosif untuk mengurangi laju korosi. Sebagian besar inhibitor yang digunakan dalam aplikasi industri adalah inhibitor anorganik. Namun inhibitor ini memiliki dampak buruk bagi ekologis dan kesehatan. Oleh karena itu diperlukan inhibitor ramah lingkungan yang berasal dari bahan alam. Dalam penelitian ini digunakan inhibitor organik yaitu daun nangka. Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak metanol daun nangka dalam menghambat laju korosi pada baja ST 37. Metode yang digunakan dalam menetukan laju korosi adalah metode kehilangan berat. Media uji korosi yaitu larutan HCl 1 M dengan variasi konsentrasi ekstrak daun nangka 0,2 ; 0,4 ; 0,6 ; 0,8, 1% dan variasi suhu 30, 40, 50, 60 ?C. Hasil uji fitokimia menunjukan bahwa ekstrak daun nangka positif mengandung senyawa alkaloid, flavonoid, fenolik, steroid, triterpenoid. Analisis FTIR menunjukkan ekstrak daun nangka memiliki gugus fungsi O-H, C=O, C=C aromatik, C-H aromatik serta mengandung senyawa alifatik golongan alkana yaitu C-H dan –CH3. Efisiensi inhibisi tertinggi yaitu 93,79% pada konsentrasi ekstrak daun nangka 1% dengan laju korosi baja 0,5682 ± 0,0067 mmpy. Sedangkan suhu optimal diperoleh pada suhu 30 ?C dengan efisiensi inhibisi tertinggi yaitu 91,63% dan laju korosi baja 0,7374 ± 0,0049 mmpy. Kata Kunci: baja ST 37, inhibitor, daun nangka, metode kehilangan berat ABSTRACT Corrosion is one of the environmental problems often found and also the center of public attention, especially in the iron and steel industries. To prevent the steel from corrosion, it is necessary to have protection from inhibitors. Corrosion inhibitors are chemicals added to a corrosive environment to reduce the rate of corrosion. Most of the inhibitors used in industrial applications are inorganic inhibitors, but these inhibitors have negative impacts on ecology and health. Therefore, we need environmentally friendly inhibitors derived from natural materials. In this study, organic inhibitors were used, namely jackfruit leaves. This study aimed to determine the effectiveness of methanol extract from jackfruit leaf in inhibiting the corrosion rate of ST 37 steel. The method used to determine the corrosion rate was the weight loss method. The corrosion test medium was 1 M HCl solution with various concentrations of jackfruit leaf extract of 0.2; 0.4; 0.6; 0.8: 1.0 %, and the temperature variations of 30, 40, 50, and 60 ?C. The results of the phytochemical test showed that jackfruit leaf extract positively contained alkaloids, flavonoids, phenolics, steroids, and triterpenoids. FTIR analysis showed that jackfruit leaf extract has the functional groups O-H, C=O, C=C aromatic, and C-H aromatic and contains aliphatic compounds of the alkane group, namely C-H and –CH3. The highest inhibition efficiency was 93.79% at a jackfruit leaf extract concentration of 1% with a steel corrosion rate of 0.5682 ± 0.0067 mmpy. Meanwhile, the optimum temperature obtained was at 30 ?C with the highest inhibition efficiency of 91.63% and the highest corrosion rate steel of 0.7374 ± 0.0049 mmpy. Keywords: inhibitor, jackfruit leaf, ST 37 steel, weight loss method.
The Dual Role of Vetiver Root Extract on Heterotrophic Bacterial Growth in Lake Riparian Zones: Implications for Lake Riparian Zone Water Quality Hermayani Sadi, Nina; Dewi, Ni Made Diah Puspita; Suirta, I Wayan
LIMNOTEK Perairan Darat Tropis di Indonesia Vol. 31 No. 1 (2025)
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/limnotek.2025.8984

Abstract

Plant–microbe interactions are fundamental to maintaining ecological balance in aquatic systems. In riparian zones, fluctuating lake water levels create standing water conditions that facilitate exchanges between riparian vegetation and aquatic microbes, particularly heterotrophic bacteria. This study examined the influence of Vetiveria zizanioides (Vetiver) root extract on heterotrophic bacterial growth in riparian ecosystems. Vetiver, widely utilized for erosion control and sediment stabilization, was selected as the experimental species. Soil and water samples from two small lakes in Cibinong, Bogor, West Java (Situ Cibuntu and Situ Cibinong), served as sources of bacterial isolates. Vetiver root extract was prepared via aqueous extraction and applied at varying concentrations in 50% tryptone glucose yeast (TGY) liquid medium. Bacterial growth responses were assessed through optical density measurements, alongside environmental parameter evaluations. Results demonstrated that Vetiver root extract modulated bacterial growth in a concentration-dependent manner: a 25% extract concentration significantly promoted heterotrophic bacterial growth, suggesting a role in enhancing nutrient cycling, whereas higher concentrations exhibited inhibitory effects, implying potential applications for microbial population management. These findings highlight the dual role of vetiver root extract in either stimulating or suppressing microbial activity within riparian ecosystems. This research provides a scientific foundation for developing nature-based solution (NBS) to promote lake ecosystem stability and environmental sustainability. Further research is needed regarding the assessment of the long-term effects of Vetiver root extract application on soil health, water quality, plant-microbe interactions, and overall ecosystem sustainability
Co-Authors Ali Sabikin Anak Agung Sagung Krisna Darmawati Dewa Ken Budiputra Dewi Novita Hardaningsih Dewi, Ni Made Diah Puspita Dwiyanti, D. A. B. E. P. Siregar E. Sahara Hermayani Sadi, Nina I Gede Tangkas Mei Yasa I Gusti Agung Gede Bawa I Gusti Ayu Kunti Sri Panca Dewi I K. G. H. Subawa I Made Dira Swantara I Made Oka Adi Parwata I Putu Eka Suwita Hegara I Wayan Sumarjaya I. A. Gede Widihati I. A. R. A. Asih I. A. R. Astiti Asih I.A.R.A. Asih Ida Bagus Putra Manuaba Kadek Agus Andika Pratama Ketut Gede Dharma Putra Ketut Ratnayani Komang Ardipa Saputra Lestari Lestari Made Arsa Minanti Arna Ekawati N. K. Gumiati N. W. Bogoriani Nasution, Dina Munawaroh Ni Komang Ariati Ni Luh Putu Kartika Mardiani Ni Luh Putu Mega Wahyuni Ni Luh Putu Putri Setianingsih Ni Luh Rustini Ni Made Puspawati P. A. Damayanti P. Suarya Putu Prisanti Putri Utami Putu Suarya S.R. Santi Saeful Bahri Sri Rahayu Santi Sri Wahjuni Taka Iqbal Prakasa W.S. Rita WAHYU DWIDJANI S Wasudewa, K. M. Wasudewa, Kresna Murti WIWIK SUSANAH RITA