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Analisis Kadar Logam Besi dalam Susu Bubuk Formula Kehamilan Secara Spektrofotometri Serapan Atom Sudiarta, I Wayan; Ratnayani, Oka; Veliyana, Ayu Kadek
Jurnal Media Sains Vol 3, No 1 (2019): JURNAL MEDIA SAINS
Publisher : LPPM Universitas Dhyana Pura

ABSTRAKLogam besi merupakan jenis mineral yang sering ditambahkan ke dalam produk susu kehamilan untuk memenuhi angka kecukupan gizi dan nutrisi bagi ibu dan janin. Analisis kadar logam besi (Fe) pada susu bubuk formula ?A? dan ?B? menggunakan Spektrofotometri Serapan Atom (AAS) dengan teknik kurva kalibrasi. Preparasi sampel menggunakan metode destruksi kering melalui pengabuan sampel pada suhu 4200C dan destruksi basah menggunakan asam kuat HNO3 dan HCl pekat untuk melarutkan sampel. Hasil penelitian menunjukkan bahwa kadar besi pada susu ?A? dan ?B? dengan metode destruksi kering dan basah secara berturut-turut 247,97 ? 0,98 mg/kg dan 397,40 ? 13,31 mg/kg untuk sampel A, 284,96 ? 4,08 mg/kg dan 517,72 ? 12,00 mg/kg untuk sampel B. Hasil validasi metode menunjukkan bahwa metode destruksi kering relatif lebih baik digunakan untuk analisis logam Fe dibandingkan dengan metode basah dengan prosentase recovery (akurasi) dan simpangan baku (presisi) 100,95 ? 0,031% dan 1,00% untuk sampel susu A, 96,79 ? 0,021% dan 0,54% untuk sampel susu B. Sedangkan dengan metode destruksi basah diperoleh prosentase recovery (akurasi) dan simpangan baku (presisi) sebesar 98,41 ? 0,67% dan 2,43% untuk sampel susu A, 93,81 ? 0,110% dan 3,20% untuk sampel B.Kata kunci: Logam besi, Destruksi Basah, Destruksi kering, Susu bubuk kehamilanABSTRACTThe iron metal is a type of mineral that is often added to pregnancy dairy products to meet the number of nutritional adequacy and nutrition for the mother and fetus. Analysis of iron (Fe) contain in milk powder of formula "A" and "B" using by atomic absorption spectrophotometry method (AAS) with calibration curve technique. Sample preparation using dry destruction was carried out by ashing the sample at 420 oC and wet destruction using the concentrated nitric and chloride acid to dissolve the sample. The results showed that iron content in milk "A" and "B" with dry destruction methods was 246.48 ? 0.99 mg / kg and 283.93 ? 4.08 mg / kg and with wet destruction of 216.66 ? 6.44 mg / kg and 257.36 ? 2.28 mg / kg. The result of method validation showed that dry destruction method was better than wet method with recovery percentage (accuracy) and standard deviation (precision) 100,95 ? 3,10% and 0,41% for milk "A", 96,76 ? 1 , 76% and 1.43% for milk "B". While the wet destruction method obtained the percentage of recovery (accuracy) and standard deviation (precision) of 97.80% ? 2.00 and 3.39% for milk "A", and 93.31% ? 0.93 and 2.34 for milk "B".Key words: Iron metal, Wet Destruction, Dry destruction, Milk powder pregnancy

KANDUNGAN Pb DAN Cr TOTAL DALAM AIR SERTA BIOAVAILABILITASNYA DALAM SEDIMEN DI PERAIRAN SUNGAI BEDADUNG JEMBER Wahyu Irwansyah; Emmy Sahara; Oka Ratnayani
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 7 No 2 (2019): volume 7, Nomor 2, 2019
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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ABSTRAK: Sungai Bedadung yang membelah kota Jember memiliki resiko terhadap pencemaran karena sungai ini digunakan sebagai tempat pembuangan sampah. Penelitian ini bertujuan untuk menentukan tingkat pencemaran dan bioavailabilitas Pb dan Cr dalam sedimen di perairan sungai Bedadung Jember. Digesti sampel dilakukan menggunakan campuran HNO3 dan HCl (3:1) dalam ultrasonic bath pada suhu 60 oC selama 45 menit dilanjutkan dengan pemanasan pada suhu 140oC selama 45 menit. Spesiasi dilakukan dengan metode ekstraksi bertahap. Konsentrasi Pb dan Cr dianalisis menggunakan Atomic Absorption Spectrofotometer (AAS) dengan metode adisi standar tunggal untuk sampel air dan kurva kalibrasi untuk sampel sedimen. Hasil penelitian menunjukkan bahwa konsentrasi Pb dan Cr total dalam air berturut-turut sebesar 0,1103 - 0,6151 mg/L dan 0,0287 – 0,1731 mg/L, sedangkan konsentrasi Pb dan Cr total dalam sedimen berturut-turut sebesar 1,7075 – 10,6026 mg/kg dan 2,5256 – 8,0493 mg/kg. Hasil spesiasi logam Pb dan Cr berturut-turut adalah sebagai berikut: fraksi EFLE sebesar td (tidak terdeteksi) hingga 6,5456 mg/kg dan 0,8662 – 3,2820 mg/kg, fraksi Fe/Mn oksida sebesar td hingga 2,6743 mg/kg dan 0,3528 – 2,8290 mg/kg, fraksi organik sulfida sebesar 0,3541 – 3,2564 mg/kg dan 0,2197 – 1,2285 mg/kg serta fraksi Resistant sebesar td hingga 3,8375 mg/kg dan 0,1111 – 0,5943 mg/kg. Dengan demikian, dapat diketahui bahwa logam Pb dan Cr yang bioavailable sebesar td hingga 61,74% dan 34,30 – 59,71%, yang berpotensi bioavailable untuk logam Pb sebesar td hingga 49,69% dan 9,77 – 35,15% untuk logam Cr, sedangkan logam Pb dan Cr yang non-bioavailable sebesar td hingga 58,68% dan 3,08 – 22,34%. Kata kunci: bioavailabilitas, Cr, Pb, sungai Bedadung Jember ABSTRACT: Bedadung River dividing the city of Jember faces the risk of pollution because of the fact that this river has become a waste disposal area. This study aimed to determine the level of pollution and bioavailability of Pb and Cr in sediments of the Bedadung Jember river. The sample digestion was carried out using the mixture of HNO3 and HCl (3:1) in an ultrasonic bath at 60 oC for 45 minutes followed by heating at 140 oC for 45 minutes. The speciation was carried out by four stages sequential extraction method. Pb and Cr concentrations in the solution were analyzed by using Atomic Absorption Spectrophotometer (AAS) with a single standard addition method for water samples and calibration curves for sediment. The results showed that the total Pb and Cr concentrations in the water were 0.1103 – 0.6151 mg/L and 0.0287 – 0.1731 mg/L, respectively, while the total Pb and Cr concentrations in the sediments were 1.7075 – 10.6026 mg/kg and 2.5256 – 8.0493 mg/kg, respectively. The results of the speciation for Pb and Cr metals were as follows: EFLE fraction of nd (undetectable) – 6.5456 mg/kg and 0.8662 – 3.2820 mg/kg, Fe/Mn oxide fraction of nd – 2.6743 mg/kg and 0.3528 – 2.8290 mg/kg, organic sulfide fraction of 0.3541 – 3.2564 mg/kg and 0.2197 – 1.2285 mg/kg and resistant fraction of nd – 3.8375 mg/kg and 0.1111 – 0.5943 mg/kg, respectively. From these results, it could be found that the bioavailable, potentially bioavailable and non-bioavailable of Pb and Cr were nd – 61.74% and 34.30 – 59.71%, nd – 49.69% and 9.77 – 35.15%; and nd – 58.68% and 3.08 – 22.34%, respectively.

FRAKSINASI SELULASE MIKROBA SELULOLITIK DENGAN AMONIUM SULFAT DAN AMOBILISASI PADA AGAR-AGAR KOMERSIAL Oka Ratnayani; Putu Elvira Yulianthi; I Nengah Wirajana
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 9 No 1 (2021): Cakra Kimia (Indonesian E-Journal of Applied Chemistry)
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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ABSTRAK: Penggunaan selulase yang semakin meningkat telah mendorong berbagai studi tentang eksplorasi, pemurnian, dan amobilisasi selulase. Penelitian ini bertujuan untuk fraksinasi ekstrak kasar selulase yang diperoleh dari mikroba selulolitik serta amobilisasinya pada agar-agar komersial. Produksi selulase dari mikroba selulolitik U3.1 dilakukan pada media yang mengandung substrat spesifik yaitu Carboxymethyl cellulose (CMC). Ekstrak kasar selulase dimurnikan dengan metode fraksinasi amonium sulfat dan dialisis. Ekstrak kasar selulase dan selulase murni diamobilisasi pada matrik agar-agar. Aktivitas selulase ditentukan berdasarkan analisis gula pereduksi yang dihasilkan dari reaksi enzimatis dengan reagen DNS. Kadar protein total diukur dengan metode Biuret. Aktivitas spesifik ekstrak kasar selulase dan selulase murni diperoleh berdasarkan rasio antara aktivitas selulase dengan kadar protein total tiap fraksi selulase. Fraksi 2 (selulase yang dimurnikan dengan garam amonium sulfat dengan tingkat kejenuhan 20-40%) memiliki aktivitas spesifik selulase tertinggi sebesar 3,3977 x 10-3 U/µg, dengan tingkat kemurnian 21,0317 kali dibandingkan ekstrak kasar selulase. Konsentrasi agar-agar optimum untuk amobilisasi ekstrak kasar selulase dan selulase murni adalah masing- masing 2% (b/v) dan 3% (b/v) dengan efisiensi amobil sebesar 13,99% dan 51,26%. ABSTRACT: The increasing use of cellulase has encouraged various studies on exploration, purification and immobilization of the enzyme. This study aimed to fractionate the crude extract of cellulase obtained from cellulolytic microbe as well as immobilize it into commercial agar. The cellulase production from cellulolytic microbe U3.1 was carried out on the media containing a specific substrate of Carboxymethyl cellulose (CMC). The crude extract of cellulase was purified by fractionation of ammonium sulfate and dialysis. The crude extract and the pure cellulase were immobilized into the agar matrix. Cellulase activity was determined based on the analysis of reducing sugars produced by enzymatic reaction with DNS reagent. The total protein content was measured by using the Biuret method. The specific activity of the crude extract and the pure cellulase was obtained from the ratio of the cellulase activity to the total protein of each fraction. Fraction 2 (cellulase purified with 20-40% saturation of ammonium sulfate) had the highest specific activity of 3.3977 x 10-3 U/µg with the purity level increased by 21.0317 times compared to the crude extract of cellulase. The optimum agar concentration for immobilization of the crude extract and the pure cellulase was 2% (b/v) and 3% (b/v) with the immobilization efficiency of 13.99% and 51.26%, respectively.

SINTESIS DAN KARAKTERISASI ARANG DARI LIMBAH BAMBU DENGAN AKTIVATOR ZnCl2 Manuntun Manurung; Oka Ratnayani; Rizgyandhaka Artha Prawira
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 7 No 1 (2019): Volume 7, Nomor 1, 2019
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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ABSTRAK: Bambu merupakan salah satu bahan baku pembuatan arang yang dapat diaktivasi secara fisika atau kimia untuk menghasilkan arang aktif. Tujuan dari penelitian ini adalah membuat dan mengkarakterisasi arang aktif dari limbah batang bambu dengan aktivator ZnCl2. Penelitian diawali dengan membuat arang bambu melalui karbonisasi pada suhu 650oC selama 90 menit. Kemudian arang diaktivasi dengan penambahan larutan ZnCl2 dengan berbagai konsentrasi. Hasil penelitian menunjukkan bahwa konsentrasi optimum aktivator ZnCl2 adalah 0,02 M. Arang aktif yang diperoleh memiliki kadar air 5,00%, kadar zat mudah menguap 6,00%, kadar abu 7,33%, kadar karbon 81,67 %, daya serap terhadap Iod 1091,426 mg/g, dan daya serap methylene blue 198,724 mg/g. Karakteristik ini telah memenuhi baku mutu SNI 06-3730-1995 tentang arang aktif teknis. Luas permukaan arang aktif sebesar 737,74 m2/g dan keasaman permukaannya sebesar 0,5122 mmol/g. Analisis gugus fungsi terhadap arang aktif menunjukkan adanya gugus fungsi O-H, karbonil, alkuna, dan ester. Kata kunci: aktivasi, karakterisasi, arang aktif, limbah bambu. ABSTRACT: Bamboo can be used as a raw material for making carbon that can be activated physically or chemically to get activated carbon. The purpose of this research was to synthesize and characterize activated carbon from bamboo stem waste by using ZnCl2 solution as activator. The research was started by preparing the bamboo carbon through carbonization at a temperature of 650oC for 90 minutes. Activation was carried out by adding ZnCl2 with various concentration ratios. The results showed that the optimum concentration of ZnCl2 activator was 0.02 M. The activated carbon had a moisture content of 5.00%, volatile substance of 6.00%, ash content of 7.33%, carbon content of 81.67 %, iodine absorbtion capacity of 1091.426 mg/g, and methylene blue absorbtion capacity of 198.724 mg/g. These characteristics had met the SNI 06-3730-1995 standard about technical activated carbon. The surface area and surface acidity of this carbon was of 737.74m2/g and 0.5122 mmol/g respectively. The functional group analysis of the activated carbon showed the presence of O-H, carbonyls, alkynes, and esthers.

SINTESIS KOMPOSIT ZnO-BENTONIT dan PENGGUNAANNYA DALAM PROSES DEGRADASI METHYL ORANGE Olivia Carolyn Sitepu; Oka Ratnayani; Iryanti Eka Suprihatin
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 4 No 2 (2016)
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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ABSTRAK: Penelitian ini bertujuan untuk menentukan kondisi optimum proses degradasi methyl orange dengan menggunakan komposit ZnO-bentonit. Komposit ZnO-bentonit telah disintesis melalui proses sonikasi menggunakan ultrasonic batch selama 3 jam. Komposit tersebut dikarakterisasi menggunakan FTIR, XRD dan SEM, dan kemudian diaplikasikan sebagai fotokatalis dalam proses degradasi methyl orange menggunakan iradiasi sinar UV. Kondisi optimumnya didapat pada pH 5, konsentrasi komposit ZnO-bentonit sebesar 10 g/L dan waktu iradiasi selama 15 menit. Pada kondisi optimum tersebut, dihasilkan degradasi methyl orange menggunakan komposit ZnO-bentonit sebesar 56,71±0,65%. ABSTRACT: This research aims to determine the optimum conditions of degradation of methyl orange by using ZnO-Bentonite composite. ZnO-bentonite composite had been synthesized through sonicationby using ultrasonic batch for 3 hours. The particles were characterized by FTIR, XRD and SEM, and then applied as photocatalyst for degradation of methyl orange with UV irradiation. The optimum conditions were obtained at pH 5, composite concentration of 10 g/L, and irradiation time of 15 minutes. The result showed that at the optimum conditions, the percentage of degradation of methyl orange by using ZnO-bentonit composite was 56,71±0,65%.

FOTODEGRADASI ZAT WARNA LIMBAH CAIR INDUSTRI PENCELUPAN DENGAN KATALIS ZEOLIT ALAM/TiO2 DAN SINAR UV Dewik Setiyawati; I Nengah Simpen; Oka Ratnayani
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 8 No 1 (2020): Volume 8, Nomor 1, 2020
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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ABSTRAK: Penelitian degradasi limbah cair industri pencelupan telah dilakukan. Dalam penelitian ini, fotodegradasi zat warna limbah cair industri pencelupan dilakukan menggunakan katalis zeolit alam/TiO2. Penelitian ini bertujuan untuk mengetahui luas permukaan spesifik katalis zeolit alam/TiO2, asam basa permukaan serta jumlah situs aktifnya, dan untuk menentukan kemampuan katalis zeolit alam/TiO2 dalam fotodegradasi zat warna limbah cair industri pencelupan terhadap perubahan pH, COD, dan intensitas zat warnanya. Karakterisasi dilakukan secara metode sorpsi methylene blue untuk mengamati luas permukaan spesifik katalis, FTIR untuk menentukan gugus fungsi yang terkandung dalam katalis, dan metode titrasi asam-basa untuk mengetahui sifat asam-basa permukaan katalis serta jumlah situs aktif. Karakteristik dengan menggunakan methylene blue diperoleh luas permukaan spesifik zeolit alam/TiO2 sebesar 164,3179 m2/g, dan jumlah situs aktif total sebesar 13,1208 x 1020 atom/g. Kondisi optimum fotodegradasi katalis zeolit alam/TiO2 terjadi pada menit ke-90 dengan massa katalis 2,0 g serta volume limbah optimum pada 25 mL dan nilai COD sebesar 145,62 mg/L dan pH 6,73. ABSTRACT: Research on the degradation of dyeing industry wastewater has been carried out. In this study, fotodegradation of dyeing industry wastewater was done using natural zeolite/TiO2 catalyst. The study aimed to determine the characteristics of natural zeolite/TiO2 catalysts such as specific surface area, acidity-basicity and amount of active sites, as well as the ability of natural zeolite/TiO2 for photodegradation of dyeing industrial wastewater based on pH, COD and the concentration of the dyes. The characterization of natural zeolite/TiO2 was carried out using methylene blue sorption method to observe the catalysts specific surface area, FTIR to analyse the functional groups contained in the catalysts, and acid-base titration methods to determine the acid-base properties of the surface as well as the number of the active sites. Characterization by using methylene blue sorption method resulted in specific surface area of zeolite/TiO2 of 164.3179 m2/g and the number of active sites was 13.1208 x 1020 atom/g. The optimum conditions for photodegradation of natural zeolite/TiO2 catalysts occurred at 90 minutes, with catalyst weight of 2.0 g, the optimum waste volume of 25 mL and COD value of 145.62 mg/L and pH 6.73.

SINTESIS DAN KARAKTERISASI ARANG DARI LIMBAH BAMBU DENGAN AKTIVATOR ZnCl2 Manuntun Manurung; Oka Ratnayani; Rizgyandhaka Artha Prawira
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 7 No 2 (2019): volume 7, Nomor 2, 2019
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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ABSTRAK: Bambu merupakan salah satu bahan baku pembuatan arang yang dapat diaktivasi secara fisika atau kimia untuk menghasilkan arang aktif. Tujuan dari penelitian ini adalah membuat dan mengkarakterisasi arang aktif dari limbah batang bambu dengan aktivator ZnCl2. Penelitian diawali dengan membuat arang bambu melalui karbonisasi pada suhu 650oC selama 90 menit. Kemudian arang diaktivasi dengan penambahan larutan ZnCl2 dengan berbagai konsentrasi. Hasil penelitian menunjukkan bahwa konsentrasi optimum aktivator ZnCl2 adalah 0,02 M. Arang aktif yang diperoleh memiliki kadar air 5,00%, kadar zat mudah menguap 6,00%, kadar abu 7,33%, kadar karbon 81,67 %, daya serap terhadap Iod 1091,426 mg/g, dan daya serap methylene blue 198,724 mg/g. Karakteristik ini telah memenuhi baku mutu SNI 06-3730-1995 tentang arang aktif teknis. Luas permukaan arang aktif sebesar 737,74 m2/g dan keasaman permukaannya sebesar 0,5122 mmol/g. Analisis gugus fungsi terhadap arang aktif menunjukkan adanya gugus fungsi O-H, karbonil, alkuna, dan ester. Kata kunci: aktivasi, karakterisasi, arang aktif, limbah bambu. ABSTRACT: Bamboo can be used as a raw material for making carbon that can be activated physically or chemically to get activated carbon. The purpose of this research was to synthesize and characterize activated carbon from bamboo stem waste by using ZnCl2 solution as activator. The research was started by preparing the bamboo carbon through carbonization at a temperature of 650oC for 90 minutes. Activation was carried out by adding ZnCl2 with various concentration ratios. The results showed that the optimum concentration of ZnCl2 activator was 0.02 M. The activated carbon had a moisture content of 5.00%, volatile substance of 6.00%, ash content of 7.33%, carbon content of 81.67 %, iodine absorbtion capacity of 1091.426 mg/g, and methylene blue absorbtion capacity of 198.724 mg/g. These characteristics had met the SNI 06-3730-1995 standard about technical activated carbon. The surface area and surface acidity of this carbon was of 737.74m2/g and 0.5122 mmol/g respectively. The functional group analysis of the activated carbon showed the presence of O-H, carbonyls, alkynes, and esthers.

KARAKTERISASI KATALIS BATU PADAS LEDGESTONE TERAKTIVASI ASAM DAN APLIKASINYA PADA PEMBUATAN BIODIESEL DARI MINYAK JELANTAH Ika Juliana; Ida Ayu Gede Widihati; Oka Ratnayani
Jurnal Kimia (Journal of Chemistry) Vol. 10, No. 1 Januari 2016
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

This research aims to improve characteristics of ledgestone catalyst by using acid activation with various concentrations. The acid used for activation was H2SO4 with concentrations of 1, 2 and 3M. The characterization of catalysts was carried out by acid-base titration method to determine the surface acidity, methylene blue adsorption to measure the spesific surface area of ??the catalyst, and the NH4OAc extraction method to measure the value of cation exchange capacity of the catalyst. Ledgestone catalyst with the best character was applied to convert waste cooking oil into biodiesel. The characterization of biodiesel was carried out by densitometry to determine the density of biodiesel, Oswald viskosimetry to measure the viscosity, and Gas Chromatography-Mass Spectrometry (GCMS) to determine the composition of chemical compounds of the biodiesel. The results showed that the concentration of acid used to activate ledgestone catalyst with the best character was 1M. This acid-activated ledgestone (catalyst A1) had a value of surface acidity, surface area, active sites number, and high cation exchange capacity were 0.3530 ± 0.0011 mmol / gram and35.7581 m2/gram, 2.1258 x 1020 atoms/gram, and 5.88 me/100 g, respectively. The concentration of catalyst A1 producing the highest biodiesel yield was 1% w/v. The produced biodiesel was 74,71% with 0.02% FFA, acid value of 0.0438 mg KOH/gram biodiesel, density of 0.7850 g/mL, kinematic viscosity of 0.4650 cSt. Two major compounds of the biodiesel were methyl hexadecanoate with area of 71.84% and cis methyl-9-octadecenoate with area of 28.16%.

IDENTIFIKASI SENYAWA AKTIF ANTIMAKAN BIJI SIRSAK (Annona muricata L.) TERHADAP ULAT KUBIS (Plutella xylostella) Lusiana Tabuni; Sri Wahjuni; Oka Ratnayani
Jurnal Kimia (Journal of Chemistry) Vol. 8, No. 2 Juli 2014
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Identification of antifeedant active compounds from Soursop seeds (Annona muricata Linn) as natural pesticides against Plutella xylostella has been carried out. 1800 g Soursop seed powder maceration was applied using methanol and n-hexane resulted in 46.42 g extract of methanol and 94.19 g extract of n-hexane. Viscous extract of methanol was diluted with the mixture of methanol:water (7:3), then evaporated until the entire methanol was removed, and after that it was partitioned with chloroform. The antifeedant activity of viscous extract of n-hexane, chloroform and water was tested which showed that the extract of n-hexane had highest activity compared to the others. Separation of 2 g viscous n-hexane extract with Coloumn Chromatography using Gel Silica 60 and n-hexane:aceton (4:1) as the mobile phase resulted in 5 fractions. The fractions were tested against Plutella xyllostella. The results showed that fraction C had the highest antifeedant activity. The isolate of fraction C was then identified using Gas Chromatography-Mass Spectrometry (GC-MS) based on the data base (Database\NISTO2.L and Database\wiley7n.1) suggested the active compounds were ethyl hexadecanoate ester and 11-octadecanoic acid.

ISOLASI SENYAWA GOLONGAN TRITERPENOID DAN UJI TOKSISITAS EKSTRAK ETANOL BATANG PRANAJIWA (Euchresta horsfieldii (Lesch) Benn) TERHADAP LARVA UDANG (Artemia salina Leach) I Wayan Gede Gunawan; Oka Ratnayani; I Putu Gede Suwindra Putra
Jurnal Kimia (Journal of Chemistry) Vol. 10, No. 2 Juli 2016
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Isolasi dan identifikasi senyawa toksik berpotensi sebagai antikanker dari batang pranajiwa (Euchresta horsfieldii (Lesch) Benn) telah dilakukan pada penelitian ini. Sebanyak 800 g sampel batang pranajiwa diekstraksi dengan 5000 mL etanol 96% menghasilkan 40,19 g ekstrak pekat etanol, dan hasil uji toksisitasnya terhadap larva udang Artemia salina Leach menunjukan LC50 79,43 ppm. Partisi ekstrak etanol dengan n-heksana, kloroform, dan air menghasilkan 1,50 g ekstrak n-heksana (LC50 50,11 ppm), 1,20 g ekstrak kloroform (LC50 630,95 ppm), dan 30,06 g ekstrak air (LC50 158,48 ppm). Ekstrak n-heksana bersifat paling toksik selanjutnya dipisahkan, dimurnikan, dan diidentifikasi. Pemisahan ekstrak n-heksana secara kromatografi kolom dengan fase diam dan fase gerak n-heksana-kloroform (5:5) menghasilkan empat fraksi (F1-F4). Fraksi F1 dengan noda tunggal pada kromatografi lapis tipis bersifat paling toksik (LC50=79,43 ppm). Hasil identifikasi fraksi F1 dengan uji fitokimia, spektrofotometer UV-Vis, dan FTIR, diduga F1 positif mengandung senyawa golongan triterpenoid.

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