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All Journal Jurnal Ilmu dan Kesehatan Hewan Buletin Veteriner Udayana Jurnal Veteriner Indonesia Medicus Veterinus Jurnal Kedokteran Hewan

Tjok Gde Oka Pemayun

Laboratorium Reproduksi Veteriner Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman Denpasar, Bali.

Author-ID : 2925606

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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INDUKSI ESTRUS DENGAN PMSG DAN GN-RH PADA SAPI PERAH ANESTRUS POSTPARTUM Tjok Gde Oka Pemayun
Buletin Veteriner Udayana Vol. 1 No. 2 Agustus 2009
Publisher : The Faculty of Veterinary Medicine, Udayana University

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This study was conducted to observe the effect of Gn-RH and PMSG on onset of oestrusin the postpartum anoestrus dairy cattle. The total of twenty one postpartum anoestrus dairycattle for used for this study. They were divided into three groups i.e. (I) treated with singledose injection of 500 ug Gn-RH /im/head (Gn-RH 1x), (II) treated with twice injection 250ug Gn-RH/im/head (at 24 hours interval) (Gn-RH 2x), and (III) treated with single doseinjection of 1000 IU PMSG/im/head. The result showed that the onset of oestrus wichtreated with PMSG was earlier (3,43 ± 0,79 days) than Gn-RH 1x (7,17 ± 3,24 days). Incoclusion, The PMSG was as effective as Gn-RH to the onset of oestrus

Penurunan Kadar Progesteron Setelah Pemberian PGF 2? Yang Diekstrak Dari Sel Monolayer Vesikula Seminalis Sapi Bali Disimpan Pada Suhu Dan Waktu Berbeda (DECREASE LEVEL OF PROGESTERONE AFTER TREATED WITH PGF 2? EXTRACTED FROM BALI CATTLE VESICULA SEMINAL Tjok Gde Oka Pemayun; I Gusti Ngurah Bagus Trilaksana; Laba Mahaputra
Buletin Veteriner Udayana Vol. 7 No. 1 Pebruari 2015
Publisher : The Faculty of Veterinary Medicine, Udayana University

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The aim of this study was to evaluate the efectiveness of PGF2? on the decrease level of progesterone after treated with PGF2? that extracted from bali cattle vesicula seminalis monolayercell that stored for 0 month, two months and four months at temperature 40C and -4oC. Sample that used in this study was PGF2? producted from bali cattle vesicula seminalis monolayer cell. Acomplete random sample with two factorial pattern was used i.e; factor I was the period stored (0month, two months, and four months) and factor II was the temperature storage (4oC).Each treatment was completely with repetitions. Result showed that PGF2? that resulted from balicattle monolayer cell vesikula seminalis that stored for two months was able to decrease level ofprogesterone to 49.06% at -4oC and 48.82% at temperature 40C. Similarly. The PGF2? that storedfor four months affected on the decrease of progesterone level to 36.33% at temperature -4C and35.81% at temperature 40C. It can be concluded that PGF2? that resulted in the study that stored forfour months at temperature of -40C and 40C may have capability to regress luteal cell.

Daya Hidup Spermatozoa Babi Large White Dengan Penambahan Ekstrak Cairan Vesikula Seminalis Sapi Bali (VITALITY OF SPERMATOZOA IN WHITE LARGE BREED SWINES ADDED BY EXTRACTED LIQUID OF BALI CATTLE SEMINAL VESICLE) Khaza Aini; Tjok Gde Oka Pemayun
Buletin Veteriner Udayana Vol. 7 No. 1 Pebruari 2015
Publisher : The Faculty of Veterinary Medicine, Udayana University

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The purposes of this study was to know the vitality of spermatozoa from large white breedswines in diluents phosphat egg yolk added by extracted liquid of bali cattle seminal vesicle storedin a temperature of four to five degree celcius. this study used completely randomized design with 4 treated group, each group repeated five times. first group were semen dilueted with phosphat eggyolk without added by extracted liquid of seminal vesicle as control; second group were semendilueted with phosphat egg yolk added by zero point five percent concentration of extracted liquidof seminal vesicle; third group were semen diluted with phosphat egg yolk added by one percentconcentration of extracted liquid of seminal vesicle, and fourth group were semen dilueted withphosphat egg yolk added by one point five percent concentration of extracted liquid of seminalvesicle. the observation was conducted to know the percentage of alive spermatozoa every 12 hour.the results of this study showed that the addition of extracted liquid of seminal vesicle wassignificant effected the vitality of spermatozoa in which the best concentration of extracted liqud od seminal vesicle was one percent.

PROFIL HEMATOLOGI SAPI BALI PADA PERIODA KEBUNTINGAN DI SENTRA PEMBIBITAN SOBANGAN BADUNG Anak Agung Sagung Kendran; Tjok Gde Oka Pemayun
Buletin Veteriner Udayana Vol. 12 No. 2 August 2020
Publisher : The Faculty of Veterinary Medicine, Udayana University

Bali cattle is germplasm that must be maintained. Various efforts have been made, such as breeding, so that the population can be improved. In the breeding, management of pregnancy will be the main thing to get attention. The gestation period in cattle is an important situation in health management. In this period, physiological status must be in good condition to ensure birth and get calf or calf, which is strong enough and able to survive. Cattle who are pregnant usually tend to weak and vulnerable diseases. This study was conducted to determine the disruption of organs or body functions earlier before showing signs of pain. Blood parameters observed from 28 cows were: The total erythrocytes, the total leukocytes, Packed Cell Volume (PCV), Hb, differential leucocytes (lymphocytes, neutrophils, basophils, eosinophils, and monocytes), Mean Corpuscular Haemoglobine (MCH), Mean Corpuscular Haemoglobine Concentration (MCHC), Mean Corpuscular Volume (MCV). Blood was taken from the jugular vein. The data obtained were analyzed by analysis of variance, with an analytical procedure using the SPSS program. The study results that the hematology of Balinese cattle at each pregnancy phase in Sobangan Badung Breeding Center is not different from non-pregnant cows.

KADAR PROGESTERON AKIBAT PEMBERIAN PMSG DAN GN-RH PADA SAPI PERAH YANG MENGALAMI ANESTRUS POSTPARTUM Tjok Gde Oka Pemayun
Buletin Veteriner Udayana Vol. 2 No. 2 Agustus 2010
Publisher : The Faculty of Veterinary Medicine, Udayana University

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This study was conducted to observe the level of progesterone associated with injectionofGn-RH and PMSG in the postpartum anoestrus dairy cattle. The total of twenty onepostpartum anoestrus dairy cattle used for this study. They were divided into three groupsi.e. (I) treated with single dose injection of 500 ug Gn-RH /im/head (Gn-RH 1x), (II)treated with twice injection of 250 ug Gn-RH/im/head (at 24 hours interval) (Gn-RH 2x),and (III) treated with single dose injection of 1000 IU PMSG/im/head. The result showedthat the concentration of progesterone was no significantly different (P > 0.05) amongPMSG and Gn-RH. However the concentration of progesterone significantly (P < 0.05)increased at 4 days on PMSG and Gn-RH treatmen. In coclusion, PMSG and Gn-RH havethe same respone to elevated of the progesterone.

Kadar dan Daya Luteolitik PGF2? Produksi Sel Monolayer Vesikula Seminalis dan Endometrium Sapi Bali (PROSTAGLANDIN F2? CONCENTRATIONS OF BALI CATTLE ENDOMETRIAL AND SEMINAL VESICLE MONOLAYER CELLS CULTURE PRODUCTS AND ITS IN VITRO TEST ON LUTEAL MONOLAYER Tjok Gde Oka Pemayun; I Gusti Ngurah Bagus Trilaksana; Laba Mahaputra
Jurnal Veteriner Vol 12 No 1 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The aims of this research were to determine PGF2? concentration the produced by bali cattlesendometrial and seminal vesicle monolayer cell culture and in vitro luteolytic ability on luteal monolayercell culture. The endometrial and seminal vesicle epithelial cell of bali cattle were cultured in tissueculture medium (TCM) 199 growth medium supplemented with 10% fetal calf serum and 10% EstrusMare Serum. The cells were cultured at 1.9 x 106 density per ml medium. Then Followed by incubation at38.50 C in 5% CO2 atmosphere for 12 days. The level of PGF2? in the cell culture medium were assayed byRadioimmnuassay (RIA) technique. The luteal cells were cultured in 9 days incubation and divided into 2groups. Group I were added with 10% of cell culture product and group II were added with 1,25 mgdinoprost/ml. The level of progesterone produced by luteal cell culture was measured at day 9th and 11thincubation. The result showed concentration of PGF2? cell product of seminal vesicle cell culture wassignificantly higher (P < 0.05) compared to endometrial cell culture. There was no significant difference(P>0.05) in luteolytic ability between PGF2? cell culture product and dinoprost. In conclusion, the PGF2?could be produced by monolayer cell culture of bali cattle is endometrial and seminal vesicle epithelialcells more over they have similar ability with dinoprost in luteolytic ability.

Waktu Inseminasi Buatan yang Tepat pada Sapi Bali dan Kadar Progesteron pada Sapi Bunting (THE OPTIMUM TIME FOR ARTIFICIAL INSEMINATION IN BALI CATTLE AND THE PROGESTERONE LEVEL IN PREGNANT COW) Tjok Gde Oka Pemayun; I Gusti Ngurah Bagus Trilaksana; Made Kota Budiasa
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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This study aims were to determine the proper time for insemination and the progesterone level ofpregnant Bali cattle. Complete randomized design method was used in this study. The study consisted ofthree treatment groups i.e. Group I, animals were inseminated at 0 hour (on estrus), group II, inseminatedat 12 h post-estrus and group III inseminated at 24 h post-estrus. Estrus was observed two times a day ie.in the morning (6:00 to 8:00 am) and afternoon (16:00 to 18:00 pm) which was characterized by transparentvaginal discharge. The results showed that the highest percentage of pregnancy occurred when the cattlewere inseminated at 24 h post-estrus (100%), however, statistically this was not significant different (P>0.05) to animals that were inseminated at 12 h post-estrus (75%). Moreover, no pregnancy (0%) wasobserved in cattle that were inseminated at estrus. The progesterone level of pregnant bali cattle increasedas the period of gestation increased, being 15.43 ± 0.50 ng/mL at 30 days of gestation, 17.16 ± 0.34 ng/mLat 60 days of gestation and 20.78 ± 0.59 ng/mL at 90 days of gestation. In conclusion, the best time forinsemination in Bali cattle is at 24 h post-estrus and progesterone level seems to increase as the older thegestation period.

Kadar Prostaglandin F2? pada Cairan Vesikula Seminalis dan Produk Sel Monolayer Vesikula Seminalis Sapi Bali (CONCENTRATIONS OF PROSTAGLANDIN F2? IN SEMINAL VESICLE FLUID AND PRODUCT OF SEMINAL VESICLE MONOLAYER CELLS OF BALI CATTLE) Tjok Gde Oka Pemayun
Jurnal Veteriner Vol 8 No 4 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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In this study, the concentration of prostaglandin F2 ? (PGF2?) in seminal vesicle fluid and seminal vesicle monolayer cell cultures of Bali cattle was determined. The seminal vesicle fluid was aspirated and the epithelial cells of the seminal vesicles were cultured in tissue culture medium (TCM) 199 growth medium containing 10% fetal calf serum (FCS) and 10% oestrus mares serum (EMS) with a density of 1.9 x 106 cells / ml medium. Following an incubation at 38.50 C in 5% CO2 atmosphere for 6 days and the level of PGF2 ? in the original seminal vesicle fluid and in the cell culture medium were determined by radioimmunoassay techniques (RIA). The results showed that the level of PGF2 ? in the non-extracted monolayer culture of seminal vesicle (1287,50 ± 3,39 pg/ml ) was significantly higher than that of detected in non-extracted seminal vesicle fluid (1,23 ± 0,79 pg/ml). In contrast, after extraction the level of PGF2 ? in seminal vesicle monolayer cell cultures (218,33 ± 2,87 pg/ml) significantly decreased as compared to seminal vesicle fluid (1750,83 ± 2,71 pg/ml). In conclusion the highest level of PGF2 ? was found in the extract of seminal vesicle fluid.

THE RESPONSE OF PGF2 ? EXTRACTION RESULT OF BALI CATTLE SEMINAL VESICLE FLUID ON PROGESTERONE LEVEL OF LUTEAL PHASE IN THE MARE Tjok Gde Oka Pemayun; Laba Mahaputra; Ismudiono -; Soetjipto -
Jurnal Veteriner Vol 9 No 4 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The study was carried out to determine the response of PGF2 ? result extraction of Bali cattle seminal vesicle fluid on the level of progesterone. The seminal vesicle fluid was aspirated and then were. extracted with methanol. This reseach was conducted by devided the mare in the luteal phase into two groups. The first group were treated with PGF2 ? result extraction of Bali cattle seminal vesicle fluid and the second group were treated with dinoprost as a patent product of PGF2 ? were administered intra uterine. The level of progesterone was measured before (0 hours) treatment and at 24, 48, 72 hours after treatment. The level of Progesterone were determined by radioimmunoassay (RIA) technique. The result showed that PGF2 ? result extraction of Bali cattle seminal vesicle liquid decreased the progesterone level 73.03% at 24 hours and 92.79% at 48 Hours. However, there was no significant different between PGF2 ? result extraction of Bali cattle seminal vesicle liquid with PGF2 ? of paten product on the progesterone level decreased. In conclusion, PGF2 ? extraction of Bali cattle seminal vesicle liquid can decreas the level of progesterone in the mare with luteal phase.

PENAMPILAN REPRODUKSI SAPI BALI PADA SISTEM TIGA STRATA Tjok Gde Oka PemayunSentana Putra2; Sentana Putra; W. Puger
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui pengaruh sistem tiga strata terhadap penampilan reproduksi sapi bali. Penelitian dalam bentuk demo plot dengan rancangan acak lengkap terdiri atas 2 sistem peternakan yakni Sistem Tiga Strata (STS) dan Sistem Tradisional (NTS) dengan 5 ulangan. Sistem Tiga Strata adalah tata cara penanaman dan pemangkasan rumput dan legum (sebagai stratum 1), semak (sebagai stratum 2), dan pohon (sebagai stratum 3), sehingga pakan hijauan tersedia sepanjang tahun untuk sapi yang selalu dikandangkan. Sistem tradisional (NTS) adalah pengembalaan sapi waktu siang hari dan pengandangan waktu malam hari dengan pemberian pakan hijauan yang dipotong dari tegalan. Estrus pascapartus dan interval beranak pada sapi STS lebih pendek dibandingkan NTS. Bobot lahir dan bobot sapih pada STS lebih berat daripada NTS, sedangkan lama kebuntingan tidak menunjukkan perbedaan nyata. Penampilan reproduksi sapi bali yang dipelihara dengan sistem pemeliharaan STS lebih baik dibandingkan dengan sistem pemeliharaan NTS.

Co-Authors Aerawata, I Wayan Gede Anak Agung Sagung Kendran Gunawan, I Wayan Nico Fajar I Gusti Ngurah Bagus Trilaksana I Ketut Puja I Ketut Suardita I Nyoman Oka Widiarta I Putu Agus Kertawirawan I Wayan Bebas I WAYAN PUTRA ARIMBAWA Ismudiono - Khaza Aini Laba Mahaputra MADE KOTA BUDIASA Made Kota Busiasa Ni Ketut Suartini Pandia, Yoga Mahendra Sentana Putra Soetjipto - W. Puger

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